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pcDNA3.1 3xFLAG::GABARAPL1

Expression vectors of human ATG8 homolog GABARAPL1 tagged with 3xFLAG at N-terminus.

Catalog number RDB19200
Resource name pcDNA3.1 3xFLAG::GABARAPL1
Clone info. Expression vectors of human ATG8 homolog GABARAPL1 tagged with 3xFLAG at N-terminus.
Comment Expression was confirmed by the depositor with co-immunoprecipitation.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human GABARAPL1 cDNA
Depositor|Developer Yoshimori, Tamotsu |
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External Database
human GABARAPL1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a separate material transfer agreement with Osaka University.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakamura, S. et al., Nat. Cell Biol., 22 (10): 1252-1263, 2020)。利用者が非営利目的の研究・教育以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19200 pcDNA3.1 3xFLAG::GABARAPL1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Nakamura, S., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol.
          22 (10): 1252-1263 (2020). PMID 32989250.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Nakamura, S., LC3 lipidation is essential for TFEB activation during the lysosomal damage response to kidney injury. Nat. Cell Biol.
          22 (10): 1252-1263 (2020). PMID 32989250.

          2022.05.16

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