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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

AAV:CT-probe-cyto-mCherry (#RDB19167)

Adeno-associated viral vector encoding the dGRAPHIC CT probe.

Clone info. Adeno-associated viral (AAV-Dj/8) vector encoding the dGRAPHIC (diffusible GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections) CT probe (split sites with the 8–11 C terminal) fragment. The cytosolic mCherry is fused to the NT probe through a self-cleavable T2A peptide. This clone is used in conjunction with AAV:NT-probe-nuc-mCherry (RDB19166) as a tool to visualize the shape of cells after cell-cell contact. For more information, see Kinoshita, N. et al. Sci. Rep., 10 (1): 14437, 2020.
Comment Expression was confirmed by the depositor with confocal imaging.
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 7.3 kb
Selectable markers Ampicillin (E. coli)
Gene/insert name mouse Acr cDNA mouse Thy1 cDNA
Depositor|Developer Shimogori, Tomomi | Kinoshita, Nagatoki |
 
Sequence (partial) RDB19167pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kinoshita, N. et al., Sci. Rep., 10 (1): 14437, 2020).
Remarks Remember that you will be working with samples containing infectious virus.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する (Kinoshita, N. et al., Sci. Rep., 10 (1): 14437, 2020)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19167 AAV:CT-probe-cyto-mCherry DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The AAV:CT-probe-cyto-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19167).

Reference section:

Kinoshita, N., Huang, A.J.Y., McHugh, T.J., Miyawaki, A., Shimogori, T., Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact. Sci. Rep. 10 (1): 14437 (2020). PMID 32879377. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19167_B1F2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: insert mid(EF-1 alpha pro,mAcr signal peptide,sfGFP-CT,T2A,mCherry 5')
Sequence file: RDB19167_B1F2a.seq check
>D07910A2_B1F2_1_EF1a-C_F_D11_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NGANTTTGGN NCTTGGTTCN TTCTCNAGCC TCAGACAGTG
   61 GTTCAAAGTT TTTTTCTTCC NTTTCAGGTG TCGTGAGGTA CCGGATCCTC TAGACACCAT
  121 GGTAGAGATG CTGCCAACTG TCGCTGTGCT GGTCTTGGCA GTGTCCGTGG TTGCCAAGGA
  181 TAACACCACG CTGCAGGCTA GAAAGAACGG CATCAAGGCG AACTTCACGG TCCGCCACAA
  241 CGTCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA
  301 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGACCGTCC TGAGCAAAGA
  361 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGGCTAG
  421 CTACCAACTC GAGAATGGCG GCATAAGCCT GCTGGTTCAG AACACATCCT GGATGCTGCT
  481 GCTGCTGCTT TCCCTCTCCC TCCTCCAAGC CCTGGACTTC ATTTCTCTGG CATGCGAGGG
  541 CAGAGGAAGT CTTCTAACAT GCGGTGACGT CGAGGAGAAT CCTGGCCCAA TGGTGAGCAA
  601 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA
  661 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
  721 GGGCACCCAG ACCGCCAAGC TGAANGTGAN CAAGGGTGGC CCCCNGCCCT TCGCCNNNNA
  781 CATCCTGTCC CCTCAGTTCA TGTACNGCTC CAANGCCTAC GTGAAGCACC NCGCCGANNT
  841 CCCCGANTAC TTGAANCTGT CCTTNCCCGA NGGCTTCNAG NGGGANCGCG NGATGANNTN
  901 NNNNGANNGN GGCGNNNNNN CNGNGNNCCN NNANTNNNCC NNNCNNNNNG NNNNNTNNNN
961 NNNNNANNNN NNNNNN
//
Primer: hGHpolyA-R (Pr0350)
Region: insert mid(hGH pA,WPRE,mCherry 3')
Sequence file: RDB19167_B1F2b.seq check
>D07910A2_B1F2_1_hGHpolyA-R_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNAN AANANTATAN NNNGNNACCT AGTCAGACAA AATGATGCAA
   61 CTTAATTTTA TTAGGNCAAG GCTGGTGGGC ACTGGAGTGG CAACTTCCAG GGCCAGGAGA
  121 GGCACTGGGG AGGGGTCACA GGGATGCCAC CCGTAGATCT CTCGAGCAGC GCTCGGTATC
  181 GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG CGAAGACGCG
  241 GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA TTGAGGGCCG
  301 AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACATAG GCGAGCAGCC
  361 AAGGAAAGGA CGATGATTTC CCCGACAACA CCACGGAATT GTCAGTGCCC AACAGCCGAG
  421 CCCCTGTCCA GCAGCGGGCA AGGCAGGCGG CGATGAGTTC CGCCGTGGCA ATAGGGAGGG
  481 GGAAAGCGAA AGTCCCGGAA AGGAGCTGAC AGGTGGTGGC AATGCCCCAA CCAGTGGGGG
  541 TTGCGTCAGC AAACACAGTG CACACCACGC CACGTTGCCT GACAACGGGC CACAACTCCT
  601 CATAAAGAGA CAGCAACCAG GATTTATACA AGGAGGAGAA AATGAAAGCC ATACGGGAAG
  661 CAATAGCATG ATACAAAGGC ATTAAAGCAG CGTATCCACA TAGCGTAAAA GGAGCAACAT
  721 AGTTAAGAAT ACCAGTCAAT CTTTCACAAA TTTTGTAATC CAGAGGTTGA TTATCGATAA
  781 GCTTGATATC CTATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG CGGCCCTCNG
  841 CGCGTTCGTA CTGNTNCCAC GATGGTGTAG NCNNCGNTNN TGGGNNGNGA TGTNNNANTT
  901 GATGTTGACG TTGTANGCGC NNGGNAGCNN CACGGNNTTC TTNGNCNNNN TANGNGGNCN
961 NGNNNNNNNC GNCNNANNNN NCNNNNNNNN NN
//
Primer: EGFP-C (Pr0025)
Region: insert mid(T2A,mCherry 5')
Sequence file: RDB19167_B1F2c.seq check
>D07910A2_B1F2_1_EGFP_C_F11_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NNNGGCGGCN TAAGCCTGCT GGTTCNGAAC ACATCCTGGN
   61 TGCTGCTGCT GCTGCTTTCC CTCTCCCTCC TCCAAGCCCT GGACTTCATT TCTCTGGCAT
  121 GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTCGA GGAGAATCCT GGCCCAATGG
  181 TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC
  241 ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC
  301 CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG
  361 CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG
  421 CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA
  481 TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG
  541 AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC
  601 AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC
  661 TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGANG
  721 TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA
  781 TCAAGTTGGA CNTCACCTCC CACAACGAGG NNTACACCNT CGTGNAACAG TACGAACGCG
  841 CCNANGGCCG CCACTCNNCC GGCGGCATGG NNGANCTGNA CAAGTAATAN GANATCAAGC
  901 TTATCGATAN TCNACCTCNN GNNNNANAAA ATTTGNGNAA GANTGNNTGG NATTCNTNAN
961 CTNNNNTNNN NNCNTTTNNN NNNNNNN
//
Primer: EF1a-M_R (Pr1023)
Region: insert 5'(EF-1 alpha pro),AAV2 ITR
Sequence file: RDB19167_B1F2d.seq check
>D07910A2_B1F2_1_EF1a-M_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTCNNGGCA CGCAAGGGCC ATAACCCGTA
   61 AAGAGGCCAG GCCCGCGGGA ACCACACACG GCACTTACCT GTGTTCTGGC GGCAAACCCG
  121 TTGCGAAAAA GAACGTTCAC GGCGACTACT GCACTTATAT ACGGTTCTCC CCCACCCTCG
  181 GGAAAAAGGC GGAGCCAGTA CACGACATCA CTTTCCCAGT TTACCCCGCG CCACCTTCTC
  241 TAGGCACCGG TTCAATTGCC GACCCCTCCC NCCNACTTCT CGGGGACTGT GGGCGATGTG
  301 CGCTCTGCCC ACTGACGGGC ACCGGAGCCA ATTCCCACTC CTTTCAAGAC CTAGAAGGTC
  361 CATTAGCTGC AAAGATTCCT CTCTGTTTAA AACTTTATCC ATCTTTGCAA AGCTTACGCG
421 TGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTN
//
Primer: mCherry-check_F (Pr0520)
Region: insert mid(mCherry 3',WPRE)
Sequence file: RDB19167_B1F2e.seq check
>D07910A2_B1F2_1_mCharry-check_F_G11_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTACNNN GGCNNCCNNN NCGCCNAGCT
   61 GAAGGTGANC AAGGGTGGCC CCNTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT
  121 GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC
  181 CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC
  241 CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG
  301 CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC
  361 CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA
  421 GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC
  481 CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA
  541 GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT
  601 GGACGAGCTG TACAAGTAAT AGGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA
  661 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA
  721 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC
  781 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGANGAGTTG TGGCCCGTTG TCANGCAACG
  841 TGGCGTGGTG TGCACTGNGT TTGCTGACGC NNCCCCCACT GGTTGGGGNC ANTGNCACCA
  901 CCNGTCANCT CCNTTNCNGG NNTTTCGCTT TCCNNNNNCN NATTGNNNCN GCNGNNNNNA
961 TNNNNNNNNG NCNNNNNNNN NN
//
Primer: f1ori_F (Pr0280)
Region: f1 ori,AAV2 ITR
Sequence file: RDB19167_B1F2f.seq check
>D07910A2_B1F2_1_f1ori_F_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANT GTAGCGGTNN NGCTGCGCGT AACCNCCACA CCCGCCGCGC
   61 TTAATGCGCC GCTACAGGGC GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC
121 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCCC TGCAGGCAGC TGCGCGCTN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kinoshita, N., Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact. Sci. Rep. 10 (1): 14437 (2020). PMID 32879377. [link to RRC of NBRP]