Please review the QC test results indicated by icon below as well as clone information before placing your order.

AAV:CT-probe-cyto-mCherry

Adeno-associated viral vector encoding the dGRAPHIC CT probe.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kinoshita, N. et al., Sci. Rep., 10 (1): 14437, 2020).
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	利用者は、研究成果の公表にあたって譲渡者の指定する文献を引用する (Kinoshita, N. et al., Sci. Rep., 10 (1): 14437, 2020)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19167_B1F2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: insert mid(EF-1 alpha pro,mAcr signal peptide,sfGFP-CT,T2A,mCherry 5') Sequence file: RDB19167_B1F2a.seq
>D07910A2_B1F2_1_EF1a-C_F_D11_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NGANTTTGGN NCTTGGTTCN TTCTCNAGCC TCAGACAGTG    61 GTTCAAAGTT TTTTTCTTCC NTTTCAGGTG TCGTGAGGTA CCGGATCCTC TAGACACCAT   121 GGTAGAGATG CTGCCAACTG TCGCTGTGCT GGTCTTGGCA GTGTCCGTGG TTGCCAAGGA   181 TAACACCACG CTGCAGGCTA GAAAGAACGG CATCAAGGCG AACTTCACGG TCCGCCACAA   241 CGTCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA   301 CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGACCGTCC TGAGCAAAGA   361 CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGGCTAG   421 CTACCAACTC GAGAATGGCG GCATAAGCCT GCTGGTTCAG AACACATCCT GGATGCTGCT   481 GCTGCTGCTT TCCCTCTCCC TCCTCCAAGC CCTGGACTTC ATTTCTCTGG CATGCGAGGG   541 CAGAGGAAGT CTTCTAACAT GCGGTGACGT CGAGGAGAAT CCTGGCCCAA TGGTGAGCAA   601 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA   661 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA   721 GGGCACCCAG ACCGCCAAGC TGAANGTGAN CAAGGGTGGC CCCCNGCCCT TCGCCNNNNA   781 CATCCTGTCC CCTCAGTTCA TGTACNGCTC CAANGCCTAC GTGAAGCACC NCGCCGANNT   841 CCCCGANTAC TTGAANCTGT CCTTNCCCGA NGGCTTCNAG NGGGANCGCG NGATGANNTN   901 NNNNGANNGN GGCGNNNNNN CNGNGNNCCN NNANTNNNCC NNNCNNNNNG NNNNNTNNNN 961 NNNNNANNNN NNNNNN //
Primer: hGHpolyA-R (Pr0350) Region: insert mid(hGH pA,WPRE,mCherry 3') Sequence file: RDB19167_B1F2b.seq
>D07910A2_B1F2_1_hGHpolyA-R_B11_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAN AANANTATAN NNNGNNACCT AGTCAGACAA AATGATGCAA    61 CTTAATTTTA TTAGGNCAAG GCTGGTGGGC ACTGGAGTGG CAACTTCCAG GGCCAGGAGA   121 GGCACTGGGG AGGGGTCACA GGGATGCCAC CCGTAGATCT CTCGAGCAGC GCTCGGTATC   181 GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG CGAAGACGCG   241 GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA TTGAGGGCCG   301 AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACATAG GCGAGCAGCC   361 AAGGAAAGGA CGATGATTTC CCCGACAACA CCACGGAATT GTCAGTGCCC AACAGCCGAG   421 CCCCTGTCCA GCAGCGGGCA AGGCAGGCGG CGATGAGTTC CGCCGTGGCA ATAGGGAGGG   481 GGAAAGCGAA AGTCCCGGAA AGGAGCTGAC AGGTGGTGGC AATGCCCCAA CCAGTGGGGG   541 TTGCGTCAGC AAACACAGTG CACACCACGC CACGTTGCCT GACAACGGGC CACAACTCCT   601 CATAAAGAGA CAGCAACCAG GATTTATACA AGGAGGAGAA AATGAAAGCC ATACGGGAAG   661 CAATAGCATG ATACAAAGGC ATTAAAGCAG CGTATCCACA TAGCGTAAAA GGAGCAACAT   721 AGTTAAGAAT ACCAGTCAAT CTTTCACAAA TTTTGTAATC CAGAGGTTGA TTATCGATAA   781 GCTTGATATC CTATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG CGGCCCTCNG   841 CGCGTTCGTA CTGNTNCCAC GATGGTGTAG NCNNCGNTNN TGGGNNGNGA TGTNNNANTT   901 GATGTTGACG TTGTANGCGC NNGGNAGCNN CACGGNNTTC TTNGNCNNNN TANGNGGNCN 961 NGNNNNNNNC GNCNNANNNN NCNNNNNNNN NN //
Primer: EGFP-C (Pr0025) Region: insert mid(T2A,mCherry 5') Sequence file: RDB19167_B1F2c.seq
>D07910A2_B1F2_1_EGFP_C_F11_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNN NNNGGCGGCN TAAGCCTGCT GGTTCNGAAC ACATCCTGGN    61 TGCTGCTGCT GCTGCTTTCC CTCTCCCTCC TCCAAGCCCT GGACTTCATT TCTCTGGCAT   121 GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTCGA GGAGAATCCT GGCCCAATGG   181 TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC   241 ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC   301 CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG   361 CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG   421 CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA   481 TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG   541 AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC   601 AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC   661 TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGANG   721 TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA   781 TCAAGTTGGA CNTCACCTCC CACAACGAGG NNTACACCNT CGTGNAACAG TACGAACGCG   841 CCNANGGCCG CCACTCNNCC GGCGGCATGG NNGANCTGNA CAAGTAATAN GANATCAAGC   901 TTATCGATAN TCNACCTCNN GNNNNANAAA ATTTGNGNAA GANTGNNTGG NATTCNTNAN 961 CTNNNNTNNN NNCNTTTNNN NNNNNNN //
Primer: EF1a-M_R (Pr1023) Region: insert 5'(EF-1 alpha pro),AAV2 ITR Sequence file: RDB19167_B1F2d.seq
>D07910A2_B1F2_1_EF1a-M_R_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTCNNGGCA CGCAAGGGCC ATAACCCGTA    61 AAGAGGCCAG GCCCGCGGGA ACCACACACG GCACTTACCT GTGTTCTGGC GGCAAACCCG   121 TTGCGAAAAA GAACGTTCAC GGCGACTACT GCACTTATAT ACGGTTCTCC CCCACCCTCG   181 GGAAAAAGGC GGAGCCAGTA CACGACATCA CTTTCCCAGT TTACCCCGCG CCACCTTCTC   241 TAGGCACCGG TTCAATTGCC GACCCCTCCC NCCNACTTCT CGGGGACTGT GGGCGATGTG   301 CGCTCTGCCC ACTGACGGGC ACCGGAGCCA ATTCCCACTC CTTTCAAGAC CTAGAAGGTC   361 CATTAGCTGC AAAGATTCCT CTCTGTTTAA AACTTTATCC ATCTTTGCAA AGCTTACGCG 421 TGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTN //
Primer: mCherry-check_F (Pr0520) Region: insert mid(mCherry 3',WPRE) Sequence file: RDB19167_B1F2e.seq
>D07910A2_B1F2_1_mCharry-check_F_G11_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTACNNN GGCNNCCNNN NCGCCNAGCT    61 GAAGGTGANC AAGGGTGGCC CCNTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT   121 GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC   181 CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC   241 CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG   301 CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC   361 CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA   421 GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC   481 CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA   541 GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT   601 GGACGAGCTG TACAAGTAAT AGGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA   661 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA   721 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC   781 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGANGAGTTG TGGCCCGTTG TCANGCAACG   841 TGGCGTGGTG TGCACTGNGT TTGCTGACGC NNCCCCCACT GGTTGGGGNC ANTGNCACCA   901 CCNGTCANCT CCNTTNCNGG NNTTTCGCTT TCCNNNNNCN NATTGNNNCN GCNGNNNNNA 961 TNNNNNNNNG NCNNNNNNNN NN //
Primer: f1ori_F (Pr0280) Region: f1 ori,AAV2 ITR Sequence file: RDB19167_B1F2f.seq
>D07910A2_B1F2_1_f1ori_F_C11_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNANT GTAGCGGTNN NGCTGCGCGT AACCNCCACA CCCGCCGCGC    61 TTAATGCGCC GCTACAGGGC GCGTACTATG GTTGCTTTGA CGTATGCGGT GTGAAATACC 121 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCCC TGCAGGCAGC TGCGCGCTN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl