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AAV:NT-probe-nuc-mCherry

Adeno-associated viral vector encoding the dGRAPHIC NT probe.

Catalog number RDB19166
Resource name AAV:NT-probe-nuc-mCherry
Clone info. Adeno-associated viral (AAV-Dj/8) vector encoding the dGRAPHIC (diffusible GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections) NT probe (split sites with the 1–7 N terminal) fragment. The nucleic-localized mCherry is fused to the NT probe through a self-cleavable T2A peptide. This clone is used in conjunction with AAV:CT-probe-cyto-mCherry (RDB19167) as a tool to visualize the shape of cells after cell-cell contact. For more information, see Kinoshita, N. et al. Sci. Rep., 10 (1): 14437, 2020.
Expression was confirmed by the depositor with confocal imaging.
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 7.3 kb
Selectable markers Ampicillin (E. coli)
Gene/insert name mouse Acr cDNA mouse Thy1 cDNA human histone H2B cDNA
Depositor|Developer Shimogori, Tomomi | Kinoshita, Nagatoki |
 
Other clones in our bank mouse Acr (NCBI Gene 11434) | mouse Thy1 (NCBI Gene 21838) | human H2BC11 (NCBI Gene 8970) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kinoshita, N. et al., Sci. Rep., 10 (1): 14437, 2020).
Ordering Please visit Information of Request for Distribution.[link] UCSD's MTA is also required. Please visit Additional Forms section.
Order form [Credit Card Payment]  [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infectious virus.
UCSD's MTA is also required. Please visit Additional Forms section.
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19166 AAV:NT-probe-nuc-mCherry Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Sequence (partial) RDB19166pka01.seq provided by the depositor

Original reference

original Kinoshita, N., Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact. Sci. Rep. 10 (1): 14437 (2020). PMID 32879377.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Kinoshita, N., Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact. Sci. Rep. 10 (1): 14437 (2020). PMID 32879377.

2021.08.31

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