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AAV:NT-probe-nuc-mCherry

Adeno-associated viral vector encoding the dGRAPHIC NT probe.

Catalog number RDB19166
Resource name AAV:NT-probe-nuc-mCherry
Clone info. Adeno-associated viral (AAV-Dj/8) vector encoding the dGRAPHIC (diffusible GPI anchored Reconstitution-Activated Proteins Highlight Intercellular Connections) NT probe (split sites with the 1–7 N terminal) fragment. The nucleic-localized mCherry is fused to the NT probe through a self-cleavable T2A peptide. This clone is used in conjunction with AAV:CT-probe-cyto-mCherry (RDB19167) as a tool to visualize the shape of cells after cell-cell contact. For more information, see Kinoshita, N. et al. Sci. Rep., 10 (1): 14437, 2020.
Expression was confirmed by the depositor with confocal imaging.
Vector backbone pAAV-MCS (plasmid)
Size of vector backbone 7.3 kb
Selectable markers Ampicillin (E. coli)
Gene/insert name mouse Acr cDNA mouse Thy1 cDNA human histone H2B cDNA
Depositor|Developer Shimogori, Tomomi | Kinoshita, Nagatoki |
Other clones in our bank

External Database
mouse Acr mouse Thy1 human histone H2B

      Reference sequence
        

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kinoshita, N. et al., Sci. Rep., 10 (1): 14437, 2020).
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      Schedule A (GFP-A) [Link]
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      RDB19166 AAV:NT-probe-nuc-mCherry DNA solution

      Ordering Information [in Japanese] [in English]
      Please review the QC test results indicated by check icon below as well as clone information before placing your order.

      References and tips

      Electronic file

      Sequence (partial) RDB19166pka01.seq provided by the depositor

      Original reference

      original Kinoshita, N., Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact. Sci. Rep. 10 (1): 14437 (2020). PMID 32879377.

      Further references such as user reports and related articles (go to bottom)

      Featured content


      Sequence information

      RIKEN BRC has sequenced portions of this material for quality test.
      Please review the QC test results indicated by check icon as well as clone information before placing your order.

      Test sheet RDB19166_B1F2p1-1.pdf check

      Nucleotide sequence of a portion of this resource (if available).

      Primer: EF1a-C_F
      Region: EF-1a pro, insert 5'
      Sequence file: RDB19166_B1F2a.seq check
      >D07910A1_B1F2_1_EF1a-C_F_D10_10_ABI24.ab1
          1 NNNNNNNNNN NNNTTTTGAN TTNGGNNCTT GGTTCNTTCT CAAGCCTCAG ACAGTGGTTC
         61 NAAGTTTTTT TCTTCCNTTT CAGGTGTCGT GAGGTACCGG ATCCTCTAGA CACCATGGTA
        121 GAGATGCTGC CAACTGTCGC TGTGCTGGTC TTGGCAGTGT CCGTGGTTGC CAAGGATAAC
        181 ACCACGCTGC AGGCTAGAGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC
        241 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGCGCGG CGAGGGCGAG
        301 GGCGATGCCA CCATCGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC
        361 GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC
        421 CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG
        481 GAGCGCACCA TCTCCTTCAA GGACGACGGC AAATACAAGA CCCGCGCCGT TGTGAAGTTC
        541 GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA CCGACTTCAA GGAGGACGGC
        601 AACATCCTGG GGCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTCTA TATCACGGCC
        661 GACAAGCAGG CTAGCTACCA ACTCGAGAAT GGCGGCATAA GCCTGCTGGT TCAGAACACA
        721 TCCTGGATGC TGCTGCTGCT GCTTTCCCTC TCCCTCCTCC AAGCCCTGGA CTTCATTTCT
        781 CTGGCATGCG AGGGCAGANG AANTCTTCTA ACATGCGGTG ACGTCNANGA NAATCCTGNN
        841 CNANTGCCAG ANCCAGCGAA NTCTGCTCCC GCCCCGAAAA ANGGCTCCNA GAANGCGGNN
      901 NNNNNNNN
      //
      Primer: mCherry-check_R5
      Region: insert 3'
      Sequence file: RDB19166_B1F2b.seq check
      >D07910A1_B1F2_1_mCherry-check_R5_C01_03_ABI08.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNGNNNCCNC NTAGCGTNNA GGAGCAACAT AGTTAAGAAT
         61 ACCAGTCAAT CTTTCACAAA TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATATC
        121 CTATTACTTG TACAGCTCGT CCATGCCGCC GGTGGAGTGG CGGCCCTCGG CGCGTTCGTA
        181 CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAGGTGATG TCCAACTTGA TGTTGACGTT
        241 GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCTTGTAG GTGGTCTTGA CCTCAGCGTC
        301 GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CTGCTTGATC TCGCCCTTCA GGGCGCCGTC
        361 CTCGGGGTAC ATCCGCTCGG AGGAGGCCTC CCAGCCCATG GTCTTCTTCT GCATTACGGG
        421 GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG TAGATGAACT CGCCGTCCTG
        481 CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC TCGAAGTTCA TCACGCGCTC
        541 CCACTTGAAG CCCTCGGGGA AGGACAGCTT CAAGTAGTCG GGGATGTCGG CGGGGTGCTT
        601 CACGTAGGCC TTGGAGCCGT ACATGAACTG AGGGGACAGG ATGTCCCAGG CGAAGGGCAG
        661 GGGGCCACCC TTGGTCACCT TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC
        721 GCCCTCGCCC TCGATCTCGA ACTCGTGGCC GTTCACGGAG CCCTCCATGT GCACCTTGAA
        781 NCGCATGAAC TCCTTGATGA TGGCCATGTT ATCCTCCTCG CCCTTGCTCA CCATGGTGGC
        841 TGGATNCTTA NCGCTGGTGN ACTTGGTGAT GGCCTTAGNA NCCTCNGANA CNGCGNGCTN
        901 GGCCNACTCC CCNNNNANCA NCANGNGCAC NGNNNNNNNG GNTCTNCCNN NNNGTGANGG
      961 NCGANCGCTN NNNGNNNNNN NNNN
      //
      Primer: VN_F1
      Region: insert mid
      Sequence file: RDB19166_B1F2c.seq check
      >D07910A1_B1F2_1_VN_F1_F10_16_ABI24.ab1
          1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC GTCTATNTNN NNGCCGNNNA GCAGGCTAGC
         61 TACCAACTCG AGAATGGCGG CATAAGCCTG CTGGTTCAGA ACACATCCTG GATGCTGCTG
        121 CTGCTGCTTT CCCTCTCCCT CCTCCAAGCC CTGGACTTCA TTTCTCTGGC ATGCGAGGGC
        181 AGAGGAAGTC TTCTAACATG CGGTGACGTC GAGGAGAATC CTGGCCCAAT GCCAGAGCCA
        241 GCGAAGTCTG CTCCCGCCCC GAAAAAGGGC TCCAAGAAGG CGGTGACTAA GGCGCAGAAG
        301 AAAGGCGGCA AGAAGCGCAA GCGCAGCCGC AAGGAGAGCT ATTCCATCTA TGTGTACAAG
        361 GTTCTGAAGC AGGTCCACCC TGACACCGGC ATTTCGTCCA AGGCCATGGG CATCATGAAT
        421 TCGTTTGTGA ACGACATTTT CGAGCGCATC GCAGGTGAGG CTTCCCGCCT GGCGCATTAC
        481 AACAAGCGCT CGACCATCAC CTCCAGGGAG ATCCAGACGG CCGTGCGCCT GCTGCTGCCT
        541 GGGGAGTTGG CCAAGCACGC CGTGTCCGAG GGTACTAAGG CCATCACCAA GTACACCAGC
        601 GCTAAGGATC CAGCCACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG
        661 GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC
        721 GAGGGCGAGG GCGAGGGCCG CCCCTACGAN GGCANCCAGA CCGCCAAGCT GAANGTGACC
        781 AAGGGTGGCC CCCTGNCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC
        841 NAGGCCTACG TNAAGCNNCC CGCCNACATC CCCNANTACT TGAANCTGTC CTTCCCNNNN
        901 GNNTTCNAGN GGGGANCGCG NGATGAACTT CNANGANNGN GGNNTNNNGA NNNNGNNCNN
      961 NNNNTNCNTC CNNNNNNNNN GNNNNNNTNN NNCTNNNNNN NNNN
      //

      Please visit Sequencing and PCR primers for primer information.


      References

      Original, user report and related articles

      original Kinoshita, N., Diffusible GRAPHIC to visualize morphology of cells after specific cell-cell contact. Sci. Rep. 10 (1): 14437 (2020). PMID 32879377.

      2021.12.27

      GNP_filter3_RDBDEP_html_211111.pl