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pCDNA-FLAG-mSIK3(S551A)

Expression vector of mouse SIK3 isoform ending at exon 14, S551A mutant.

Catalog number RDB19165
Resource name pCDNA-FLAG-mSIK3(S551A)
Clone info. Expression vector of mouse salt inducible kinase 3 (SIK3) isoform ending at exon 14 having a mutation S551A tagged with 3xFLAG at N-terminus. This mutation prevents binding of 14‐3‐3 protein.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), G418 (mammalian cell)
Gene/insert name mouse Sik3 cDNA
Depositor|Developer Funato, Hiromasa |
 
Other clones in our bank mouse Sik3 (NCBI Gene 70661) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Park, M. et al., Sci. Rep., 10 (1): 8676, 2020).
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19165 pCDNA-FLAG-mSIK3(S551A) Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Park, M., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Park, M., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359.

2021.08.31

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