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pCDNA-FLAG-mSIK1(S577A)

Expression vector of mouse SIK1, S577A mutant.

Catalog number RDB19161
Resource name pCDNA-FLAG-mSIK1(S577A)
Clone info. Expression vector of mouse salt inducible kinase 1 (SIK1) having a mutation S577A tagged with 3xFLAG at N-terminus. This mutation prevents binding of 14‐3‐3 protein.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), G418 (mammalian cell)
Gene/insert name mouse Sik1 cDNA
Depositor|Developer Funato, Hiromasa |
 
Other clones in our bank mouse Sik1 (NCBI Gene 17691) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Park, M. et al., Sci. Rep., 10 (1): 8676, 2020).
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19161 pCDNA-FLAG-mSIK1(S577A) Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Park, M., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Park, M., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359.

2021.08.31

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