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Expression vector of mouse SIK1, S577A mutant.

Catalog number RDB19161
Resource name pCDNA-FLAG-mSIK1(S577A)
Clone info. Expression vector of mouse salt inducible kinase 1 (SIK1) having a mutation S577A tagged with 3xFLAG at N-terminus. This mutation prevents binding of 14‐3‐3 protein.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), G418 (mammalian cell)
Gene/insert name mouse Sik1 cDNA
Depositor|Developer Funato, Hiromasa |
Sequence (full) RDB19161hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19161_B1Erp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Park, M. et al., Sci. Rep., 10 (1): 8676, 2020).
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MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Park, M. et al., Sci. Rep., 10 (1): 8676, 2020)。
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提供同意書 (MTA, 非営利学術目的用)[Word]

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19161 pCDNA-FLAG-mSIK1(S577A) DNA solution

How to cite this biological resource

Materials & Methods section:

The pCDNA-FLAG-mSIK1(S577A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19161).

Reference section:

Park, M., Miyoshi, C., Fujiyama, T., Kakizaki, M., Ikkyu, A., Honda, T., Choi, J., Asano, F., Mizuno, S., Takahashi, S., Yanagisawa, M., Funato, H., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


Original, user report and related articles

original Park, M., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359. [link to RRC of NBRP]