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pCDNA-FLAG-mSIK1(S577A)

Expression vector of mouse SIK1, S577A mutant.

Catalog number RDB19161
Resource name pCDNA-FLAG-mSIK1(S577A)
Clone info. Expression vector of mouse salt inducible kinase 1 (SIK1) having a mutation S577A tagged with 3xFLAG at N-terminus. This mutation prevents binding of 14‐3‐3 protein.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), G418 (mammalian cell)
Gene/insert name mouse Sik1 cDNA
Depositor|Developer Funato, Hiromasa |
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External Database
mouse Sik1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Park, M. et al., Sci. Rep., 10 (1): 8676, 2020).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Park, M. et al., Sci. Rep., 10 (1): 8676, 2020)。
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          RDB19161 pCDNA-FLAG-mSIK1(S577A) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Park, M., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Park, M., Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need. Sci. Rep. 10 (1): 8676 (2020). PMID 32457359.

          2022.05.16

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