Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(L507R) (#RDB19024)

Retroviral expression vector of human KLF4, mutant L507R.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (L507R) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19024 pMXs-3xFLAG-KLF4(L507R) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(L507R) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19024).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19024_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(L507R) 5')
Sequence file: RDB19024_B1Eca.seq check
>D07716C3_B1Ec_1_MMLV-pol_F_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNNGG NNTCGNAGCT TGGNNACNCG CCGCCCNCGT GAAGGCTGCC
   61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
  121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG
  181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT
  241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA
  301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC
  361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG
  421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG
  481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG
  541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC
  601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG
  661 GCGTGGCGCC GGGCGGCACG GGCGGAGGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC
  721 CGACGGCTCC CTTCAACCTG GCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG
  781 NCGANCTCCT GCGGCCAGAA TTGGACCCGG NGTACATTCC GCCGCANCAG CCGCAGCCGC
  841 CNGGTGGCGG GCTGATGGGC AANTTCGTGC TGAANGNGTC GCTGANCGCC CCTGGCAGCG
  901 AGTACNGNAG CCCGTCNGNC NTCAGCGNCA GNNAAGGNAG NCCTGANGGC AGCCNCNNGN
  961 NGGNGGNGGC NCCNNACANC NGNGGGNCNN NNNGCNNGNG CCCNNNGATN NANNNNNNNN
 1021 GNNNNNNNCT TNNNGNNNCC NNNNGGNNNN NNGNNCCCCN NNNCNNCNNN NGNNNNNNNN
1081 NN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(attB2,hKLF4(L507R)3')
Sequence file: RDB19024_B1Ecb.seq check
>D07716C3_B1Ec_1_pMXs-3LTRup_R2_B09_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNCGTCNNN TNNNNNAGTA CATAATGGNN TTCCTTACGC
   61 GAAATACGGG CAGACATGGC CTGCCCGGTT ATTATTATTT TTGACACCAG ACCAACTGGT
  121 AATGGTAGCG ACCGGCGCTC AGCTGGAATA TCACCACTTT GTACAAGAAA GCTGGGTCGG
  181 CGCGCCCACC CTTTTAAAAA TGTCTCTTCA TGTGTCGGGC GAGGTGGTCC GACCTGGAAA
  241 ATGCTCGGTC GCATTTTTGG CACTGGAACG GGCGGTGCCC CGTGTGTTTA CGGTAGTGCC
  301 TGGTCAGTTC ATCTGAGCGG GCGAATTTCC ATCCACAGCC GTCCCAGTCA CAGTGGTAAG
  361 GTTTCTCACC TGTGTGGGTT CGCAGGTGTG CCTTGAGATG GGAACTCTTT GTGTAGGTTT
  421 TGCCGCAGCC CGCGTAATCA CAAGTGTGGG TGGCGGTCCT TTTCCGGGGC CACGATCGTC
  481 TTCCCCTCTT TGGCTTGGGC TCCTCTGGCA TGCAGGAACC GGGTGGCATG AGCTCTTGGT
  541 AATGGAGCGG CGGGACTTGC GGCTGCATCT GATCGGGCAG GAAGGATGGG TAATTGGGCC
  601 CCGGGTGGGG ATGGAAGCCG GGAGGAAGCG GCAGGGCAGG GTGACAGTCC CTGCTGCTCA
  661 GCACTTCCTC AAGANCCANG GTCGGGGTAG TCCTGCTGGG GAGCTGCCGC CCCANGGGGA
  721 AGTCGTGTGC AGCCGGCCGG TGGCCATTGC TGANAGGGGG TCCAGCGCNC AAGNGGGTGC
  781 ACGAAGANAN CGCCTCCTGC TNGATCTTGG GGCACGTGCG CGGCGGCCCG CCNTTGTNNG
  841 GCGCCACCAC CACNNGGNGG NTGNCGTCNN GNCTGCCNTN GCTGANGCNN ANGANNNANN
901 GGNNNNNNNA NNNNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR,MMLV psi
Sequence file: RDB19024_B1Ecc.seq check
>D07716C3_B1Ec_1_Amp_R_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNTN ATNNGGGTTA TTGTCTCNTG AGCGGANACA
   61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
  121 TGCCACCTGC ATAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC
  181 ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAA AAGTTCAGAT CAAGGTCAGG
  241 AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC
  301 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT
  361 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC
  421 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC
  481 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC
  541 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATTGA
  601 CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG
  661 GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT
  721 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC
  781 CGGGAGGTAA NCTGGNCAGC AACTTATCTG TGTCTGTCCG ATNGTCTAGT GTCTATGACT
  841 GANTTTATGC GCCTGCGTCG GTNCTAGTTA GCTAACTANC TCTGTATCTG GNGNANNNGT
  901 GGTGGANCTG ACGANTNNGN AACACCCGNN NGCNNNCCNN NGNGANNTCC CNNGNNNTTC
  961 NGGGGNNGTT TTTGNGGCCC GNNNNGANNC NAAAAATCCC GATCNNTTTG NNNNNTTNNN
1021 NGNNNNNNCC NTNNNNNNNN NN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19024_B1Ecd.seq check
>D07716C3_B1Ec_1_gag-N_R_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNTTNNNAN NAATACNGAA ACACAGTCAG
   61 ACAGAGACAA CACAGAACGA TGCTGCAGCA GACAAGACGC GCGGCGCGGC TTCGGTCCCA
  121 AACCGAAAGC AAAAATTCAG ACGGAGGCGG GAACTGTTTT AGGTTCTCGT CTCCTACCAG
  181 AACCACATAT CCCTCCTATT AGGGGGGTGC ACCAAAGAGT CCAAAACGAT CGGGATTTTT
  241 GGACTCAGGT CGGGCCACAA AAACGGCCCC CGAAGTCCCT GGGACGTCTC CCAGGGTTGC
  301 GGCCGGGTGT TCCGAACTCG TCAGTTCCAC CACGGGTCCG CCAGATACAG AGCTAGTTAG
  361 CTAACTAGTA CCGACGCAGG CGCATAAAAT CAGTCATAGA CACTAGACAA TCGGACAGAC
  421 ACAGATAAGT TGCTGGCCAG CTTACCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG
  481 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCG CTGACGGGTA GTCAATCACT
  541 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
  601 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC GCCGAGTGAG
  661 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
  721 GAACTGATTG GTTAGTTCAA ATAANGCACA GGGTCATTTC NGGTCCTTGG GGCACCCTGN
  781 AAACATCTGA TGGTTCTCTA GAAACTGCTG ANGGCTGGAC CGCATCTGGG GACCATCTGT
  841 TCTTGGCCCT GAGCCGGGGC ANGAACTGCT NANNNCAGAT ATCCTGNTNG GNCCNTATNC
  901 NNCTGTTCCN TCTGTTCTTG NNCNTGANCC NGGGNNNNNA ACTGCTTANC ACANATNTCC
  961 NGNTNNNNCC ATATNCNGCN NNNCNNTCTG NNNNNGANNN NGATCNGAAC TTTNCNANNN
1021 TCNNTNANGN NTTTTTNNNN NNNTNNNNAA NNNNNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19024_B1Ece.seq check
>D07716C3_B1Ec_1_lacIpro-B_F_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNCTA NNNNNNNNNT
   61 NNNNTAAATA CNNNNNANNA AAAACCNNNT TAATTTTGAN ACNNGCTGCA TGTGAAAGAC
  121 CCCCGCTGAC GGGTANTCAA TCACTCNNAG GAGACCCTCC CAAGGAACAG CGAGACCACA
  181 NGTCGGATGC AACTGCNAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCANTCAA
  241 TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAN
  301 AANCGCGCGA ACNNNNNNGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC
  361 ATTTCAGGTN NNNNNGNNNN NNTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC
  421 TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA
  481 CAGATATCCT GTTTGGCCCA TATTCNNCTG NNNNNNCTGT TCTTGGCCCT GAGCCGGGGC
  541 AGGAACTGCN NNNNNCANAT ATCCTGTTTG GNCCNNATTC NNCTGTTCCA TCTGTTCCTG
  601 ANNNTGNNNN NNNNTTCTCT ATTCTCAGNN NNNNATTTTT CCATGCCTTG NAAAATGGCG
  661 TTACNTTNNN CTAGCTTGCN NNNNNNACAG GTGGGGGTCT TTCNTTCCCC CCTTTTTCTG
  721 GANACTAANT AAANNCNTNT ATTTTATCGN CNNNNNNNAA NTANTACATA ATGNANTTNC
781 CTNANNNCNA ANNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]