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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(H504A) (#RDB19022)

Retroviral expression vector of human KLF4, mutant H504A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (H504A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19022 pMXs-3xFLAG-KLF4(H504A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(H504A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19022).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19022_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(H504A) 5')
Sequence file: RDB19022_B1Eca.seq check
>D07716C1_B1Ec_3_MMLV-pol_F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNNTCGNA NCTTGGNNAC NCGCCGCCCN CGTGAAGGCT
   61 GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCGGATC TAGCTAGTTA ATTAAGGATC
  121 CCAGTGTGGT GGTACGGGAA TTCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
  181 ATGACATCGA CTACAAGGAT GACGATGACA AGGCTGTCAG CGACGCGCTG CTCCCATCTT
  241 TCTCCACGTT CGCGTCTGGC CCGGCGGGAA GGGAGAAGAC ACTGCGTCAA GCAGGTGCCC
  301 CGAATAACCG CTGGCGGGAG GAGCTCTCCC ACATGAAGCG ACTTCCCCCA GTGCTTCCCG
  361 GCCGCCCCTA TGACCTGGCG GCGGCGACCG TGGCCACAGA CCTGGAGAGC GGCGGAGCCG
  421 GTGCGGCTTG CGGCGGTAGC AACCTGGCGC CCCTACCTCG GAGAGAGACC GAGGAGTTCA
  481 ACGATCTCCT GGACCTGGAC TTTATTCTCT CCAATTCGCT GACCCATCCT CCGGAGTCAG
  541 TGGCCGCCAC CGTGTCCTCG TCAGCGTCAG CCTCCTCTTC GTCGTCGCCG TCGAGCAGCG
  601 GCCCTGCCAG CGCGCCCTCC ACCTGCAGCT TCACCTATCC GATCCGGGCC GGGAACGACC
  661 CGGGCGTGGC GCCGGGCGGC ACGGGCGGAN GCCTCCTCTA TGGCAGGGAG TCCGCTCCCC
  721 CTCCGACGGC TCCCTTCNAC CTGGCGGANA TCAACGACGT GAGCCCCTCG GGCGGNTTCN
  781 TGGCCNANCT CCTGCGGCCN GAATTGGANC CGGNGTACNT TCCGCCGCAN CAGCCGCAGC
  841 CNNCAGGTGG CGGGCTGATG GGCAAGTTCG TGCTGAANGN GTCNCTGANC GCCCCTGNNN
  901 NNNAGNANGG NAGCCCGNCN GNCATCAGCG NCNNCAAAGG NAGNCCTGAN NNNNNNNNCC
  961 CNGNNNNGGN GGCGCCCNAN ANCNGCGGGN CNNCNCGNNC GTGCCNCNNG NTCANNCNNN
 1021 NNNNNGNNNN TNNNNGNNCC NNCNTNGNNN NNNGNNNCCC NNNNCNNNNN NGNNNNNGNN
1081 NNGNNNNNNN NNN
//
Primer: hKLF4-mut check_R2 (Pr1009)
Region: insert 3'(hKLF4(H504A) 3'),3' LTR
Sequence file: RDB19022_B1Ecb.seq check
>D07716C1_B1Ec_3_hKLF4-mut check_R2_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNT NNNNNNNNCC GGGGCCCNNT TACCCATCCT
   61 TCCTGCCCGA TCAGATGCAG CCGCAAGTCC CGCCGCTCCA TTACCAAGAG CTCATGCCAC
  121 CCGGTTCCTG CATGCCAGAG GAGCCCAAGC CAAAGAGGGG AAGACGATCG TGGCCCCGGA
  181 AAAGGACCGC CACCCACACT TGTGATTACG CGGGCTGCGG CAAAACCTAC ACAAAGAGTT
  241 CCCATCTCAA GGCACACCTG CGAACCCACA CAGGTGAGAA ACCTTACCAC TGTGACTGGG
  301 ACGGCTGTGG ATGGAAATTC GCCCGCTCAG ATGAACTGAC CAGGCACTAC CGTAAACACA
  361 CGGGGCACCG CCCGTTCCAG TGCCAAAAAT GCGACCGAGC ATTTTCCAGG TCGGACGCCC
  421 TCGCCTTACA CATGAAGAGG CATTTTTAAG CGGCCGCCAG CACAGTGGTC GACGATAAAA
  481 TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT
  541 GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA
  601 GAGAAGTTCA GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA
  661 TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA NGGCCAAGAA CAGATGGAAC AGCTGAATAT
  721 GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCANGGCC AAGAACAGAT
  781 GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG TTTCTAGAGA ACCATCANAN GTTTNCNGGG
  841 NGCCCCNAGG ACCTGAAATG ANCCTGTGCC NTATTTGAAC TANCCNANCA NTTCGCTTCN
  901 CGCTTCTGTT CNCGCGCTNC NGNNCCCCNA NNTNNNTAAA AGANCCNNNN ACCCNNCACT
961 CNGGGNNNNC NNNNNNNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert mid(hKLF4(H504A) mid)
Sequence file: RDB19022_B1Ecc.seq check
>D07716C1_B1Ec_3_pMXs-3LTRup_R2_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCGTCN NNNNCTGNGC TGGCGGCCGC TTAAAANTGC
   61 CTCTTCNTGT GTAANGCGAG GGCGTCCGAC CTGGAAAATG CTCGGTCGCA TTTTTGGCAC
  121 TGGAACGGGC GGTGCCCCGT GTGTTTACGG TAGTGCCTGG TCAGTTCATC TGAGCGGGCG
  181 AATTTCCATC CACAGCCGTC CCAGTCACAG TGGTAAGGTT TCTCACCTGT GTGGGTTCGC
  241 AGGTGTGCCT TGAGATGGGA ACTCTTTGTG TAGGTTTTGC CGCAGCCCGC GTAATCACAA
  301 GTGTGGGTGG CGGTCCTTTT CCGGGGCCAC GATCGTCTTC CCCTCTTTGG CTTGGGCTCC
  361 TCTGGCATGC AGGAACCGGG TGGCATGAGC TCTTGGTAAT GGAGCGGCGG GACTTGCGGC
  421 TGCATCTGAT CGGGCAGGAA GGATGGGTAA TTGGGCCCCG GGTGGGGATG GAAGCCGGGA
  481 GGAAGCGGCA GGGCAGGGTG ACAGTCCCTG CTGCTCAGCA CTTCCTCAAG ACCCAGGGTC
  541 NNNNNNGTCC TGCTGGGGAG CTGCCGCCCC AGGGGGAAGT CGTGTGCAGC CGGCCGGTGG
  601 CCATTGCTGA GAGGGGGTCC AGCGCCCAAG TGGGTGCACG AAGAGACCGC CTCCTGCTTG
  661 ATCTTGGGGC ACGTGCGCGG CGGCCCGCCG NTGTANGGCG CCACCACCAC CGGGTGGCTG
  721 CCGTCAGGGC TGCCTTTGCT GANGCTGATG ACCGACGGGC TGCCGTACTC GCTGCCAGGG
  781 GCGCTCAGCG ACGCCTTCAG CACGAACTTG CCCNATCAGC CCGCCACCTG NCGGCTGCGG
  841 CTGCTGCNGC GNAATGTACA CCNGGGTCCA ATTNTGGCCG CANGANCNCG GCCNCGANNC
  901 CGCCNNANGG GGCNCACNNC NNTNNANGTC CGCCANGNNN GAANNNNANC NNNNNGNNNG
961 GGGNNCNNNN NNCCNNNNNN NNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19022_B1Ecd.seq check
>D07716C1_B1Ec_3_gag-N_R_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TTNNGANNAA TANCNGAAAN CACAGTCAGA
   61 CAGANACNNA CACAGAACGN NNNNNTGCAG CAGACAAGAC GCGCGGCGCG GCTTCGGTCC
  121 CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT TTAGGTTCTC GTCTCCTACC
  181 AGAACCACAT ATCCCTCCTA TTAGGGGGGT GCACCAAAGA GTCCAAAACG ATCGGGATTT
  241 TTGGACTCAG GTCGGGCCAC AAAAACGGCC CCCGAAGTCC CTGGGACGTC TCCCAGGGTT
  301 GCGGCCGGGT GTTCCGAACT CGTCAGTTCC ACCACGGGTC CGCCAGATAC AGAGCTAGTT
  361 AGCTAACTAG TACCGACGCA GGCGCATAAA ATCAGTCATA GACACTAGAC AATCGGACAG
  421 ACACAGATAA GTTGCTGGCC AGCTTACCTC CCGGTGGTGG GTCGGTGGTC CCTGGGCAGG
  481 GGTCTCCCGA TCCCGGACGA GCCCCCAAAT GAAAGACCCC CGCTGACGGG TAGTCAATCA
  541 CTCAGAGGAG ACCCTCCCAA GGAACAGCGA GACCACAAGT CGGATGCAAC TGCAAGAGGG
  601 TTTATTGGAT ACACGGGTAC CCGGGCGACT CAGTCAATCG GAGGACTGGC GCGCCGAGTG
  661 AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG GGAGCAGAAG CGCGCGAACA GAAGCGAGAA
  721 GCGAACTGAT TGGTTAGTTC AAATAAGGCA CANGGTCATT TCANGTCCTT GGGGCANCCT
  781 GGAANCATCT GATGGTTCTC TANAAACTGC TGANGGCTGG ACCGCATCNG GGGGANCATC
  841 TGTTCTTGGC CNNGANNCNG GGGCANGAAC TGCNTNACCA NNGATATCCN GNTTNGNNCN
  901 NTATTCNNCT NNNCCATCTG TTCNTNGNNC NNNANNNGGG NNNNNNNANN GCTNANNNCN
961 NNNNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19022_B1Ece.seq check
>D07716C1_B1Ec_3_lacIpro-B_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN ANNNNNNNNN NNCNNNGAGC GAGGAAGCGG AAGANCGCCC
   61 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAACT ATGGCCATTT
  121 AATGTAAATA CTTAAGAAAA AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC
  181 CCCCGCTGAC GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA
  241 AGTCGGATGC AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA
  301 TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG
  361 AAGCGCGCGA ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC
  421 ATTTCAGGTC CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC
  481 TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA
  541 CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGC
  601 AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCCTG
  661 ACCTTGATCT GAACTTCTCT ATTCTCAGTT ATGTATTTTT CCNTGCCTTG CAAAATGGCG
  721 TTACTTAAGC TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTCCCCCC TTTTTCTGGA
  781 GACTAAATNA AANTCTTTTA TTTTATCGTC NACCACTGNG CTGGCGGNNN CTTAAAAATG
  841 CCTCTTCATG TGTANGCGAG GGCNNNCGAC CTNNNAATGN TCGGTCGNNT TTTTGNNACT
  901 GNAACGGGCG GNGCCCCNNG NGTTTACGGN AGNGNCTGGN CAGTTCNTCN NANCGGGNCN
  961 AATTTNCNTC CNNANNCGTC CCANNCNCAG NGGNAANGGN TNNTCNNCNG TGNGGGNTCN
 1021 NNNNNGNNNC NTGNNNANNG GANNNNNNNT NNGNNNNTTT TGNNNNNNNC CNNNNNNANN
 1081 NNNNANNGNG GNNNGNNGGT NNNTTTNCNN GGNNNNNNNN NCNNNNNNNN CCNNNNNNTT
1141 NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]