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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(D503A) (#RDB19021)

Retroviral expression vector of human KLF4, mutant D503A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (D503A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19021 pMXs-3xFLAG-KLF4(D503A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(D503A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19021).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19021_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(D503A)5')
Sequence file: RDB19021_B1Eca.seq check
>D07716B9_B1Ec_3_MMLV-pol_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTCGNNN NCTTGGNNAC NCGCCGCCCN CGTGAAGGCT
   61 GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCGGATC TAGCTAGTTA ATTAAGGATC
  121 CCAGTGTGGT GGTACGGGAA TTCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
  181 ATGACATCGA CTACAAGGAT GACGATGACA AGGCTGTCAG CGACGCGCTG CTCCCATCTT
  241 TCTCCACGTT CGCGTCTGGC CCGGCGGGAA GGGAGAAGAC ACTGCGTCAA GCAGGTGCCC
  301 CGAATAACCG CTGGCGGGAG GAGCTCTCCC ACATGAAGCG ACTTCCCCCA GTGCTTCCCG
  361 GCCGCCCCTA TGACCTGGCG GCGGCGACCG TGGCCACAGA CCTGGAGAGC GGCGGAGCCG
  421 GTGCGGCTTG CGGCGGTAGC AACCTGGCGC CCCTACCTCG GAGAGAGACC GAGGAGTTCA
  481 ACGATCTCCT GGACCTGGAC TTTATTCTCT CCAATTCGCT GACCCATCCT CCGGAGTCAG
  541 TGGCCGCCAC CGTGTCCTCG TCAGCGTCAG CCTCCTCTTC GTCGTCGCCG TCGAGCAGCG
  601 GCCCTGCCAG CGCGCCCTCC ACCTGCAGCT TCACCTATCC GATCCGGGCC GGGAACGACC
  661 CGGGCGTGGC GCCGGGCGGC ACGGGCGGAG GCCTCCTCTA TGGCAGGGAG TCCGCTCCCC
  721 CTCCGACGGC TCCCTTCAAC CTGGCGGACA TCAACGACGT GAGCCCCTCG GGCGGCTTCG
  781 TGGNCGAGCT CCTGCGGNCA GANNTGNANC CGGTGTACAT TCCGCCGCAG CAGCCGCAGC
  841 CGCCAGGTGG CGGGCTGATG GGCAAGTTCG TGCTGAAGGN GTCGCTGANC GCCCCTGGCA
  901 NCGAGTACNG GCAGCCNNTC NGNCATCAGC GTCANNNAAG GNANNCNTGA NGGCANCCAC
  961 CCGGNGGTGG NGGNGCCCTA NNACGGNGGN NNNNNGCGCN NGTNCCCCNN NANNANNCNN
 1021 NANNNNGNNN CNTNNNGNNN CCNNNTTGGN NNNNTNNNNC CCNTCNCNNN NANNNGNNNN
1081 NNNNNN
//
Primer: hKLF4-mut check_R2 (Pr1009)
Region: insert 3'(hKLF4(D503A) 3'),3' LTR
Sequence file: RDB19021_B1Ecb.seq check
>D07716B9_B1Ec_3_hKLF4-mut check_R2_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCNGGGGNCC NATTACCCAT CCTTCCTGCC
   61 CGATCAGATG CAGCCGCAAG TCCCGCCGCT CCATTACCAA GAGCTCATGC CACCCGGTTC
  121 CTGCATGCCA GAGGAGCCCA AGCCAAAGAG GGGAAGACGA TCGTGGCCCC GGAAAAGGAC
  181 CGCCACCCAC ACTTGTGATT ACGCGGGCTG CGGCAAAACC TACACAAAGA GTTCCCATCT
  241 CAAGGCACAC CTGCGAACCC ACACAGGTGA GAAACCTTAC CACTGTGACT GGGACGGCTG
  301 TGGATGGAAA TTCGCCCGCT CAGATGAACT GACCAGGCAC TACCGTAAAC ACACGGGGCA
  361 CCGCCCGTTC CAGTGCCAAA AATGCGACCG AGCATTTTCC AGGTCGGCCC ACCTCGCCTT
  421 ACACATGAAG AGGCATTTTT AAGCGGCCGC CAGCACAGTG GTCGACGATA AAATAAAAGA
  481 TTTTATTTAG TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC
  541 TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT
  601 TCAGATCAAG GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG
  661 TAAGCAGTTC CTGCCCCGGC TCANGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA
  721 ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCANN GCCAAGAACA GATGGTCCCC
  781 ANATGCGGNC CAGCCCTCAN CAGTTTCTAG AGAACCATCA NATGTTTCCA GNGTGCCCCA
  841 AGGANCTGAA ATGANCCTGT GCCTTATTTG AACTAACNNA TCAGTTCGCT TCTCGCTTCN
  901 GTNNNCGCGC TNNTGCTCCC CNANCTNNAN NAAANANCCC ACNNNCCCTC ACTNNGGGGC
  961 GNCNNNNCNN NNNNNGANTG ANNNNNNNNG NNNNCCNNNN NNNCNNNNAA NNCNNNNNNN
1021 NNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert mid(hKLF4(D503A) mid)
Sequence file: RDB19021_B1Ecc.seq check
>D07716B9_B1Ec_3_pMXs-3LTRup_R2_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCGNNNNNN GTGCTNNGCG GCCGCTTAAA
   61 AATGCCTCTT CATGTGTAAG GCGAGGTGGG CCGACCTGGA AAANGNTCGG TCGCATTTTT
  121 GGCACTGGAA CGGGCGGTGC CCCGTGTGTT TACGGTAGTG CCTGGTCAGT TCATCTGAGC
  181 GGGCGAATTT CCATCCACAG CCGTCCCAGT CACAGTGGTA AGGTTTCTCA CCTGTGTGGG
  241 TTCGCAGGTG TGCCTTGAGA TGGGAACTCT TTGTGTAGGT TTTGCCGCAG CCCGCGTAAT
  301 CACAAGTGTG GGTGGCGGTC CTTTTCCGGG GCCACGATCG TCTTCCCCTC TTTGGCTTGG
  361 GCTCCTCTGG CATGCAGGAA CCGGGTGGCA TGAGCTCTTG GTAATGGAGC GGCGGGACTT
  421 GCGGCTGCAT CTGATCGGGC AGGAAGGATG GGTAATTGGG CCCCGGGTGG GGATGGAAGC
  481 CGGGAGGAAG CGGCAGGGCA GGGTGACAGT CCCTGCTGCT CAGCACTTCC TCAAGACCCA
  541 GGGTCGGGGT AGTCCTGCTG GGGAGCTGCC GCCCCAGGGG GAAGTCGTGT GCAGCCGGCC
  601 GGTGGCCATT GCTGAGAGGG GGTCCAGCGC CCAAGTGGGT GCACGAAGAG ACCGCCTCCT
  661 GCTTGATCTT GGGGCACGTG CGCGGCGGCC CGCCGTTGTA GGGCGCCACC ACCACCGGGT
  721 GGCTGCCGTC AGGGCTGCCT TTGCTGACGC TGATGACCGA CGGGCTGCCG TACTCGCTGC
  781 CNNNGGCGCT CAGCGACGCC TTCAGCACGA ACTTGCCCAT CAGCCCGCCA CCTGGCGGCT
  841 GCGGCTGCTG CGGNGNAATG TNNACCNGGT CNNATTCTGG NCGNNNNGAN CTCGGNCNNC
901 NNNNNNNNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19021_B1Ecd.seq check
>D07716B9_B1Ec_3_gag-N_R_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNNNNAATT TTCNNANNAA TACNGAAACA CAGTCAGACA
   61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC
  121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC
  181 CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA
  241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC
  301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA
  361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA
  421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT
  481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG
  541 AGGAGACCCT CCCAAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT
  601 TGGATACACG GGTACCCGGG CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG
  661 TTGTGGGCTC TTTTATTGAG CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA
  721 CTGATTGGTT AGTTCAAATA AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGAAA
  781 CATCTGATGG TTCTCTAGAA ACTGCTGANG GCTGGACCGC ATCTGGGGAN CATCTGTTCT
  841 TGGCCCTGAG CCGGGGCANG AACTGCTTAN CACAGATATC CTGTTTGGNC CATATTCNGC
  901 TGTTCCNTCT GNNCTTGGNC CNGANCCGGG GCNNNNANNG CTNACCACAG ATATCCNGNT
  961 NGGNCCATAT TNNGCNGTTN CNTCTGNTNN NGANNNNGNN NNGNNNTTTT CTATTNNCAN
1021 NNNTGNNNTT TNNNNNNNNN N
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19021_B1Ece.seq check
>D07716B9_B1Ec_3_lacIpro-B_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGC ANCGNGTCNG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC
   61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT
  121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG
  181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG
  241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA
  301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG
  361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC
  421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC
  481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT
  541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA
  601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT
  661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT
  721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA
  781 AATAAAATCT TTTATTTTAT CGTCGACCAC TGTGCTGGCG GCCGCTTAAA AATGCCTCTT
  841 CATGTGTAAG GNGAGGTGGG CCGACCTGNA AAATGCTCGG TCGCATTTTT GGNACTGGAA
  901 CGGGCGGNGC CCCGTGTGTT TACGGNAGTG NCNNNNCAGT TCATCTGANC GGGCGAATTT
  961 CCATCCNCAN NNNTCCCANN CACAGNGNNN NNNNTTCNCN NNNGNNTGGG NTCGCNNGNG
 1021 NGNCNTNNAN ANGGGNNCNN NTNNNNNNNN NTTNNNNNNN NNCNNNNNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]