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pMXs-3xFLAG-KLF4(R479A) (#RDB19015)

Retroviral expression vector of human KLF4, mutant R479A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (R479A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19015 pMXs-3xFLAG-KLF4(R479A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(R479A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19015).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19015_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region, insert 5'(3xFLAG,hKLF4(R479A) 5')
Sequence file: RDB19015_B1Eca.seq check
>D07716B3_B1Ec_2_MMLV-pol_F_B06_06_ABI24.ab1
    1 NNNNNNNNNN NANNNNNNGG NATCGNANCT TGGNNNCNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGANCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
  121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG
  181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT
  241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA
  301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC
  361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG
  421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG
  481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG
  541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC
  601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG
  661 GCGTGGCGCC GGGCGGCACG GGCGGANGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC
  721 CGACGGCTCC CTTCAACCTG NCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG
  781 CCGANCTCCT GCGGCCAGAA TTGGANCCGG TGTACATTCC GCCGCANCAG CCGCANCCGC
  841 CAGGTGGCGG GCTGATGGGN CAAGTTCGTG CTGAAGGCGN CGCTGANCGC CCCNGGCANC
  901 GNNTACNGNN GCCNGNCNNN CATCAGCNNC AGCNAAGGNA GNCNNNNNNG NNNNCNACCN
961 NGNGGNNGNN GNNNNNNNNN N
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4(R479A) 3')
Sequence file: RDB19015_B1Ecb.seq check
>D07716B3_B1Ec_2_pMXs-3LTRup_R2_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCGTCGN NNNCTNNGCT GGCGGCCGCT TAAAAATGCC
   61 TCTTCATGTG TAAGGCGAGG TGGTCCGACC TGGAAAATGC TCGGTCGCAT TTTTGGCACT
  121 GGAACGGGCG GTGCCCCGTG TGTTTACGGT AGTGCGCGGT CAGTTCATCT GAGCGGGCGA
  181 ATTTCCATCC ACAGCCGTCC CAGTCACAGT GGTAAGGTTT CTCACCTGTG TGGGTTCGCA
  241 GGTGTGCCTT GAGATGGGAA CTCTTTGTGT AGGTTTTGCC GCAGCCCGCG TAATCACAAG
  301 TGTGGGTGGC GGTCCTTTTC CGGGGCCACG ATCGTCTTCC CCTCTTTGGC TTGGGCTCCT
  361 CTGGCATGCA GGAACCGGGT GGCATGAGCT CTTGGTAATG GAGCGGCGGG ACTTGCGGCT
  421 GCATCTGATC GGGCAGGAAG GATGGGTAAT TGGGCCCCGG GTGGGGATGG AAGCCGGGAG
  481 GAAGCGGCAG GGCAGGGTGA CAGTCCCTGC TGCTCAGCAC TTCCTCAAGA CCCAGGGTCG
  541 GGGTAGTCCT GCTGGGGAGC TGCCGCCCCA GGGGGAAGTC GTGTGCAGCC GGCCGGTGGC
  601 CATTGCTGAN AGGGGGTCCA GCGCCCAAGT GGGTGCACGA AGAGACCGCC TCCTGCTTGA
  661 TCTTGGGGCA CGTGCGCGGC GGCCCGCCGT TGTANGGCGC CACCACNACC GGGTGGCTGC
  721 CGTCAGGGCT GCCTTTGCTG ACGCTGATGA CCGACGGGCT GCCGTANTCG CTGCCAGGGG
  781 CGCTCAGCGA CGCCTTCAGC ACGAACTTGC CCATCAGCCC GCCACCTGGC GGCTGCNGCT
  841 GCTGCGGCNG AATGTACACC GNGTCCNNTT CTGGCCGCNN NNNNNNGCCN CNAANCCGCC
  901 CGANGGGCTN ACGNNNTTNA TGTNCNNNNN NTTGNANGGN NNCCNNNNGA NGGGGANNNN
961 NNTNCNNGNC NNANNNGNNN NNNNCGNNCN NNNNNNCNNN NNNNNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19015_B1Ecc.seq check
>D07716B3_B1Ec_2_gag-N_R_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNNTTTT NNGACNAATA CNGAAACNCA GTCAGACAGA
   61 GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC GCGGCTTCGG TCCCAAACCG
  121 AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT CTCGTCTCCT ACCAGAACCA
  181 CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA ACGATCGGGA TTTTTGGACT
  241 CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC GTCTCCCAGG GTTGCGGCCG
  301 GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA TACAGAGCTA GTTAGCTAAC
  361 TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA GACAATCGGA CAGACACAGA
  421 TAAGTTGCTG GCCAGCTTAC CTCCCGGTGG TGGGTCGGTG GTCCCTGGGC AGGGGTCTCC
  481 CGATCCCGGA CGAGCCCCCA AATGAAAGAC CCCCGCTGAC GGGTAGTCAA TCACTCAGAG
  541 GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC AACTGCAAGA GGGTTTATTG
  601 GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT GGCGCGCCGA GTGAGGGGTT
  661 GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA GAAGCGAACT
  721 GATTGGTTAG TTCAAATAAG GCACAGGGTC ATTTCANGTC CTTNGGGCAC CCTGGAAACA
  781 TCTGATGGTT CTCTAGAAAC TGCTGANGGC TGGACCGCAT CTGGGGACNA TCTGTTCTTG
  841 GCCCTGANCC GGGGCANGAA NTGCTTANCA CAGATATCCN GTTTGGCCCN TATTCAGCTG
  901 TNNCATCTGN NCNTGNNCNN NANCCNGGGN NNNANNNGNN TANNNNANNN NTCCNNNTNN
961 GNCNNTATTC NGCTNNTNNN NNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19015_B1Ecd.seq check
>D07716B3_B1Ec_2_lacIpro-B_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CANCGNNNCN GTGNNCGANG AAGCGGAAGA NCGCCCAATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG
  121 TAAATACTTA AGAAAAAAAA CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC
  181 GCTGACGGGT AGTCAATCAC TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC
  241 GGATGCAACT GCAAGAGGGT TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG
  301 AGGACTGGCG CCCCGAGTGA GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC
  361 GCGCGAACAG AAGCGAGAAG CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT
  421 CAGGTCCTTG GGGCACCCTG GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA
  481 CCGCATCTGG GGACCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA
  541 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA
  601 ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT
  661 TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTTCCAT GCCTTGCAAA ATGGCGTTAC
  721 TTAAGCTAGC TTGCCAAACC TACAGGTGGG GTCTTTCATT CNCCCCTTTT TCTGGAGACT
  781 AAATAAAATC TTTTATTTTA TCGTCNACCA CTGTGCTGGC GGCCGCTTAA AAATGCCTCT
  841 TCATGTGTAA GGNGAGGTGG TCCNANCTGN AAANNGCTCG GTCGCNTTTT TGGNACTGGA
  901 ACGGGCGGNG CCCCNNGTGT TTANGGTAGT GCGCGGNCAG TTCATCTGAN CNGGCGNANT
961 TTCCNTNCNN NNNCNNNNCC NNNCNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]