Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(D475A) (#RDB19013)

Retroviral expression vector of human KLF4, mutant D475A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (D475A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19013 pMXs-3xFLAG-KLF4(D475A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(D475A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19013).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19013_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(D475A) 5')
Sequence file: RDB19013_B1Eca.seq check
>D07716B1_B1Ec_2_MMLV-pol_F_H04_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNTCGCAN CTTGGNNACN CGCCGCCCAC GTGNAGGCTG
   61 CCGACCCCGG GGGTGGACCA TCCTCTAGAC TGCCGGATCT AGCTAGTTAA TTAAGGATCC
  121 CAGTGTGGTG GTACGGGAAT TCATGGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA
  181 TGACATCGAC TACAAGGATG ACGATGACAA GGCTGTCAGC GACGCGCTGC TCCCATCTTT
  241 CTCCACGTTC GCGTCTGGCC CGGCGGGAAG GGAGAAGACA CTGCGTCAAG CAGGTGCCCC
  301 GAATAACCGC TGGCGGGAGG AGCTCTCCCA CATGAAGCGA CTTCCCCCAG TGCTTCCCGG
  361 CCGCCCCTAT GACCTGGCGG CGGCGACCGT GGCCACAGAC CTGGAGAGCG GCGGAGCCGG
  421 TGCGGCTTGC GGCGGTAGCA ACCTGGCGCC CCTACCTCGG AGAGAGACCG AGGAGTTCAA
  481 CGATCTCCTG GACCTGGACT TTATTCTCTC CAATTCGCTG ACCCATCCTC CGGAGTCAGT
  541 GGCCGCCACC GTGTCCTCGT CAGCGTCAGC CTCCTCTTCG TCGTCGCCGT CGAGCAGCGG
  601 CCCTGCCAGC GCGCCCTCCA CCTGCAGCTT CACCTATCCG ATCCGGGCCG GGAACGACCC
  661 GGGCGTGGCG CCGGGCGGCA CGGGCGGANG CCTCCTCTAT GGCAGGGAGT CCGCTCCCCC
  721 TCCGACGGCT CCCTTCAACC TGGCGGACAT CAACGACGTG AGCCCCTCGG GCGGCTTCGT
  781 GGCCGANCTC CTGCGGCCAG AATTGGACCC NGTGTACATT CCGCCGNANC ANCCGCANCC
  841 GCCAGGTGGC GGGCTGATGG GCAAGTTCGT GCTGNANGNG TCGCTNANCG CCCCTGGCAN
  901 CGANTANGGN ANCCCGTCNG TCATCNNCGN NNNCNNANGN NANNCNNGNN NGGNAGCCAN
961 CCNNNNGGNN NNGGNGCCNN ANNNCNGNNG GNNNNNNNNN N
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4(D475A) 3')
Sequence file: RDB19013_B1Ecb.seq check
>D07716B1_B1Ec_2_pMXs-3LTRup_R2_G04_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNCGTCGNN NNCTGTGCTG GCGGCCGCTT AAAAATGCCT
   61 CTTCATGTGT AAGGCGAGGT GGTCCGACCT GGAAAATGCT CGGTCGCATT TTTGGCACTG
  121 GAACGGGCGG TGCCCCGTGT GTTTACGGTA GTGCCTGGTC AGTTCAGCTG AGCGGGCGAA
  181 TTTCCATCCA CAGCCGTCCC AGTCACAGTG GTAAGGTTTC TCACCTGTGT GGGTTCGCAG
  241 GTGTGCCTTG AGATGGGAAC TCTTTGTGTA GGTTTTGCCG CAGCCCGCGT AATCACAAGT
  301 GTGGGTGGCG GTCCTTTTCC GGGGCCACGA TCGTCTTCCC CTCTTTGGCT TGGGCTCCTC
  361 TGGCATGCAG GAACCGGGTG GCATGAGCTC TTGGTAATGG AGCGGCGGGA CTTGCGGCTG
  421 CATCTGATCG GGCAGGAAGG ATGGGTAATT GGGCCCCGGG TGGGGATGGA AGCCGGGAGG
  481 AAGCGGCAGG GCAGGGTGAC AGTCCCTGCT GCTCAGCACT TCCTCAAGAC CCAGGGTCGG
  541 GGTAGTCCTG CTGGGGAGCT GCCGCCCCAG GGGGAAGTCG TGTGCAGCCG GCCGGTGGCC
  601 ATTGCTGAGA GGGGGTCCAG CGCCCAAGTG GGTGCACGAA GAGACCGCCT CCTGCTTGAT
  661 CTTGGGGCAC GTGCGCGGCG GCCCGCCGTT GTAGGGCGCC ACCACCACCG GGTGGCTGCC
  721 GTCAGGGCTG CCTTTGCTGA CGCTGATGAN CGACGGGCTG CCGTACTCGC TGCCANGGGC
  781 GCTCAGCGAC GCCTTCAGCA CGAACTTGCC CATCAGCCCG CCACCTGGCG GCTGCGGCTG
  841 CTGCGGCGGA NTGTACACCG GGGNCCNATT CTGGCCGCNN GANCTCNGNC NNNAAGCCNC
  901 CCNANGGGCT CACGTCNNTN ATGNCCNCCA GGTTGAANGN NNCCNNNNNA NGGGGANCGN
961 NNTCCCTGNC NTANANNANN NNNNNNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19013_B1Ecc.seq check
>D07716B1_B1Ec_2_gag-N_R_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TTTTNNGANN NNNNACNGAA ACACAGTCAG
   61 ACAGAGACAA CACAGAACGA TGCTGCAGCA GACAAGACGC GCGGCGCGGC TTCGGTCCCA
  121 AACCGAAAGC AAAAATTCAG ACGGAGGCGG GAACTGTTTT AGGTTCTCGT CTCCTACCAG
  181 AACCACATAT CCCTCCTATT AGGGGGGTGC ACCAAAGAGT CCAAAACGAT CGGGATTTTT
  241 GGACTCAGGT CGGGCCACAA AAACGGCCCC CGAAGTCCCT GGGACGTCTC CCAGGGTTGC
  301 GGCCGGGTGT TCCGAACTCG TCAGTTCCAC CACGGGTCCG CCAGATACAG AGCTAGTTAG
  361 CTAACTAGTA CCGACGCAGG CGCATAAAAT CAGTCATAGA CACTAGACAA TCGGACAGAC
  421 ACAGATAAGT TGCTGGCCAG CTTACCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG
  481 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCG CTGACGGGTA GTCAATCACT
  541 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
  601 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGANTGGCGC GCCGAGTGAG
  661 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
  721 GAACTGATTG GTTAGTTCAA ATAANGCACA GGGTCATTTC ANGTCCTTGG GGCACCCTGN
  781 AAACATCTGA TGGTTCTCTA GAAACTGCTG ANGGCTGGNN CGCATCNGGG GACCATCTGT
  841 TCTTGNNCCT GANNNGGGGC ANGAACTGCT NACCNCANAT ATCCNGTTTG GCCCATATTN
  901 NGCTGTTCCA TCTGNNCTTG GCCCTGANCC NGGGNNNNNA NNNCNTNNCN NANATATNCN
961 GNTTNGNNCN NNNNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19013_B1Ecd.seq check
>D07716B1_B1Ec_2_lacIpro-B_F_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNNTCNG TGAGCGAGGA ANCGGAANAN CGCCCAATAC
   61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT
  121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG
  181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG
  241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA
  301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG
  361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC
  421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC
  481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT
  541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA
  601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT
  661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT
  721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA
  781 AATAAAATCT TTTATTTTAT CGTCGANCAC TGTGCTGGCG GCCGCTTAAA AAATGCCTCT
  841 TCATGTGTAA NGCGANGTGG TCCGANNNGN AAAATGNTCG GTCGCATTTT TTGGCACTGG
  901 ANCGGGCGGN GCCCCNNGNG NTTACGGNAN NGCCNGGNCA GTTCANCNGA NCGGGNNNNT
961 TTCCNTNNNN NGCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]