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pMXs-3xFLAG-KLF4(F471A) (#RDB19011)

Retroviral expression vector of human KLF4, mutant F471A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (F471A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19011 pMXs-3xFLAG-KLF4(F471A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(F471A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19011).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19011_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(F471A) 5')
Sequence file: RDB19011_B1Eca.seq check
>D07716A8_B1Ec_2_MMLV-pol_F_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCGCANC TTGGNNACNC GCCGCCCACG TGAAGGCTGC
   61 CGACCCCGGG GGTGGACCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCC
  121 AGTGTGGTGG TACGGGAATT CATGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT
  181 GACATCGACT ACAAGGATGA CGATGACAAG GCTGTCAGCG ACGCGCTGCT CCCATCTTTC
  241 TCCACGTTCG CGTCTGGCCC GGCGGGAAGG GAGAAGACAC TGCGTCAAGC AGGTGCCCCG
  301 AATAACCGCT GGCGGGAGGA GCTCTCCCAC ATGAAGCGAC TTCCCCCAGT GCTTCCCGGC
  361 CGCCCCTATG ACCTGGCGGC GGCGACCGTG GCCACAGACC TGGAGAGCGG CGGAGCCGGT
  421 GCGGCTTGCG GCGGTAGCAA CCTGGCGCCC CTACCTCGGA GAGAGACCGA GGAGTTCAAC
  481 GATCTCCTGG ACCTGGACTT TATTCTCTCC AATTCGCTGA CCCATCCTCC GGAGTCAGTG
  541 GCCGCCACCG TGTCCTCGTC AGCGTCAGCC TCCTCTTCGT CGTCGCCGTC GAGCAGCGGC
  601 CCTGCCAGCG CGCCCTCCAC CTGCAGCTTC ACCTATCCGA TCCGGGCCGG GAACGACCCG
  661 GGCGTGGCGC CGGGCGGCAC GGGCGGAGGC CTCCTCTATG GCAGGGAGTC CGCTCCCCCT
  721 CCGACGGCTC CCTTCAACCT GGCGGANATC AACGACGTGA GCCCCTCGGG CGGCTTCGTG
  781 GCCGANCTCC TGCGGCCAGA ATTGGACCCG GNGTACATTC CGCCGCANCA NCCGCAGCCG
  841 CCAGGTGGCG GGCTGATGNG CAAGTTCGTG CTGAANGNGT CNCTGANCGC CCCTNNNANC
  901 GANNACGGNN NCCCGNCNGN CATCANCGTC AGCAANNGNA NNCNNNANNN NNNCCNCCCN
961 GNNNNNNNNN N
//
Primer: hKLF4-mut check_R2 (Pr1009)
Region: insert 3'(hKLF4(F471A) 3'),3' LTR
Sequence file: RDB19011_B1Ecb.seq check
>D07716A8_B1Ec_2_hKLF4-mut check_R2_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NNNNNNNNNG GGGCCCNNTT ACCCATCCTT CCTGCCCGAT
   61 CAGATGCAGC CGCAAGTCCC GCCGCTCCAT TACCAAGAGC TCATGCCACC CGGTTCCTGC
  121 ATGCCAGAGG AGCCCAAGCC AAAGAGGGGA AGACGATCGT GGCCCCGGAA AAGGACCGCC
  181 ACCCACACTT GTGATTACGC GGGCTGCGGC AAAACCTACA CAAAGAGTTC CCATCTCAAG
  241 GCACACCTGC GAACCCACAC AGGTGAGAAA CCTTACCACT GTGACTGGGA CGGCTGTGGA
  301 TGGAAAGCCG CCCGCTCAGA TGAACTGACC AGGCACTACC GTAAACACAC GGGGCACCGC
  361 CCGTTCCAGT GCCAAAAATG CGACCGAGCA TTTTCCAGGT CGGACCACCT CGCCTTACAC
  421 ATGAAGAGGC ATTTTTAAGC GGCCGCCAGC ACAGTGGTCG ACGATAAAAT AAAAGATTTT
  481 ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC
  541 TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG AGAAGTTCAG
  601 ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
  661 CAGTTCCTGC CCCGGCTCAN GGCCAAGAAC AGATGGAACA GCTGAATATG GGCCAAACAG
  721 GATATCTGTG GTAAGCAGTT CCTGNCCCGG CTCNNGGCCA AGAACAGATG GTCCCCAGAT
  781 GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA CCATCAGANG TTTCCAGGGT GCCCCANNGA
  841 CCTGAAATGA CCCTGTGNNT TATTTGAACT ANCCAATCAG TTCGCTTCTC GCTTCTGNTC
  901 GCGCGCTTCT GNTCCCCGAN CNNNNTAAAA GANCCCACNN NCNCTCANNC NGGGNNNNNN
961 NNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert mid(hKLF4(F471A) mid)
Sequence file: RDB19011_B1Ecc.seq check
>D07716A8_B1Ec_2_pMXs-3LTRup_R2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTNN NCGTCGNNNN CTGTGCTGGC GGCCGCTTAA AAATGCCTCT
   61 TCNTGTGTAA GGCGAGGTGG TCCGACCTGG AAAATGCTCG GTCGCATTTT TGGCACTGGA
  121 ACGGGCGGTG CCCCGTGTGT TTACGGTAGT GCCTGGTCAG TTCATCTGAG CGGGCGGCTT
  181 TCCATCCACA GCCGTCCCAG TCACAGTGGT AAGGTTTCTC ACCTGTGTGG GTTCGCAGGT
  241 GTGCCTTGAG ATGGGAACTC TTTGTGTAGG TTTTGCCGCA GCCCGCGTAA TCACAAGTGT
  301 GGGTGGCGGT CCTTTTCCGG GGCCACGATC GTCTTCCCCT CTTTGGCTTG GGCTCCTCTG
  361 GCATGCAGGA ACCGGGTGGC ATGAGCTCTT GGTAATGGAG CGGCGGGACT TGCGGCTGCA
  421 TCTGATCGGG CAGGAAGGAT GGGTAATTGG GCCCCGGGTG GGGATGGAAG CCGGGAGGAA
  481 GCGGCAGGGC AGGGTGACAG TCCCTGCTGC TCAGCACTTC CTCAAGACCC AGGGTCGGGG
  541 TAGTCCTGCT GGGGAGCTGC CGCCCCAGGG GGAAGTCGTG TGCAGCCGGC CGGTGGCCAT
  601 TGCTGAGAGG GGGTCCAGCG CCCAAGTGGG TGCACGAAGA GACCGCCTCC TGCTTGATCT
  661 TGGGGCACGT GCGCGGCGGC CCGCCGTTGT AGGGCGCCAC CACCACCGGG TGGCTGCCGT
  721 CAGGGCTGCC TTTGCTGACG CTGATGACCG ACGGGCTGCC GTACTCGCTG CCAGGGGCGC
  781 TCAGCGACGC CTTCNGCACG AACTTGCCCA TCAGCCCGCC ACCTGGCGGC TGCNNNTGCT
  841 GCGGCNNAAN GTACACCNGG NCCNATTCTG GNCGCANGAN CTCGGCCNCN AAGCCGCCCG
901 ANGGGNTCAC GTNNTTGATG NNCGCNNNGT TGNANNNNNN NNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19011_B1Ecd.seq check
>D07716A8_B1Ec_2_gag-N_R_H03_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNG NNNNNAATTT TNNGANNNNN ANNGAAACNC AGTCAGACAG
   61 AGACAACACA GAACGATGCT GCAGCAGACA AGACGCGCGG CGCGGCTTCG GTCCCAAACC
  121 GAAAGCAAAA ATTCAGACGG AGGCGGGAAC TGTTTTAGGT TCTCGTCTCC TACCAGAACC
  181 ACATATCCCT CCTATTAGGG GGGTGCACCA AAGAGTCCAA AACGATCGGG ATTTTTGGAC
  241 TCAGGTCGGG CCACAAAAAC GGCCCCCGAA GTCCCTGGGA CGTCTCCCAG GGTTGCGGCC
  301 GGGTGTTCCG AACTCGTCAG TTCCACCACG GGTCCGCCAG ATACAGAGCT AGTTAGCTAA
  361 CTAGTACCGA CGCAGGCGCA TAAAATCAGT CATAGACACT AGACAATCGG ACAGACACAG
  421 ATAAGTTGCT GGCCAGCTTA CCTCCCGGTG GTGGGTCGGT GGTCCCTGGG CAGGGGTCTC
  481 CCGATCCCGG ACGAGCCCCC AAATGAAAGA CCCCCGCTGA CGGGTAGTCA ATCACTCAGA
  541 GGAGACCCTC CCAAGGAACA GCGAGACCAC AAGTCGGATG CAACTGCAAG AGGGTTTATT
  601 GGATACACGG GTACCCGGGC GACTCAGTCA ATCGGAGGAC TGGCGCGCCG AGTGAGGGGT
  661 TGTGGGCTCT TTTATTGAGC TCGGGGAGCA GAAGCGCGCG AACAGAAGCG AGAAGCGAAC
  721 TGATTGGTTA GTTCAAATAA GGCACAGGGT CATTTCAGGT CCTTGGGGCA CCCTGGAAAC
  781 ATCTGATGGN TCTCTAGAAA CTGCTGANGG CTGGACCGCA TCTGGGGANC ATCTGTTCTT
  841 GGCCCTGANN NGGGGNANGA ACTGCTNANC ACAGATATCC TGTTTNGCCN ATATTNNGCT
  901 GNTCCATCNG NTCTTGGCCC NNANNNNGGG GCNNNNNCTG NNNANCNNNG ATATNCNNNT
961 TNGNNNNNNN NNCNNNNGNT NNNNNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19011_B1Ece.seq check
>D07716A8_B1Ec_2_lacIpro-B_F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NCAGTGANCG AGGNAGCGGN ANNAGCGCCC
   61 NATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGANNNNN TAATGCAACT ATGGCCATTT
  121 NNNGTAAATA CTTAAGAAAA AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC
  181 CCCCGCTGAC GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA
  241 AGTCGGATGC AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA
  301 TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG
  361 AAGCGCGCGA ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC
  421 ATTTCAGGTC CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC
  481 TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA
  541 CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGC
  601 AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCCTG
  661 ACCTTGATCT GAACTTCTCT ATTCTCAGTT ATGTATTTTT CCATGCCTTG CAAAATGGNG
  721 TTACTTAAGC TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTCCCCCC TTTTTCTGGA
  781 GACTAAATAA AATCTTTTAT TTTATCGTCN ACCACTGTGC TGGCGGCCGC TNNANNAATG
  841 CCTCTTCATG TGTAANGCGA GGTGGNNCGA NCNTGNAAAA TGCTCNGGTC GCATTTTTGN
  901 NANTGGNAAN NGGGCGGTNN CCNNNTGTGT TTANNGGTAN TNNCNGGNNC NNNTNNNTNN
  961 NGANNNGGCN GNNTTTNCNA TCCNNAGCNN NTCCCANTCN NANNGGNAAN GGNTTNNNNN
1021 NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]