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pMXs-3xFLAG-KLF4(H450A) (#RDB19010)

Retroviral expression vector of human KLF4, mutant H450A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (H450A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19010 pMXs-3xFLAG-KLF4(H450A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(H450A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19010).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19010_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(H450A) 5')
Sequence file: RDB19010_B1Eca.seq check
>D07716A7_B1Ec_8_MMLV-pol_F_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNTCGCANCT TGGNNACNCG CCGCCCACGN GAAGGCTGCC
   61 GACCCCGGGG GTGGANCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
  121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG
  181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT
  241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA
  301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC
  361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG
  421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG
  481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG
  541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC
  601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG
  661 GCGTGGCGCC GGGCGGCACG GGCGGANGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC
  721 CGACGGCTCC CTTCAACCTG GCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG
  781 CCGANCTCCT GCGGCCAGAA TTGGACCCGG TGTACNTTCC GCCGCANCAG CCGCAGCCGC
  841 CAGGTGGCGG GCTGATGGGN AAGTTCGTGC TGAANGNGTC GCTNANCGCC CCTNGNANCG
901 ANNNNNNNNN N
//
Primer: hKLF4-mut check_R2 (Pr1009)
Region: insert 3'(hKLF4(H450A) 3)',3'LTR
Sequence file: RDB19010_B1Ecb.seq check
>D07716A7_B1Ec_8_hKLF4-mut check_R2_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNN NGGGGCCCNN TTACCCATCC TTCCTGCCCG
   61 ATCAGATGCA GCCGCAAGTC CCGCCGCTCC ATTACCAAGA GCTCATGCCA CCCGGTTCCT
  121 GCATGCCAGA GGAGCCCAAG CCAAAGAGGG GAAGACGATC GTGGCCCCGG AAAAGGACCG
  181 CCACCCACAC TTGTGATTAC GCGGGCTGCG GCAAAACCTA CACAAAGAGT TCCCATCTCA
  241 AGGCAGCCCT GCGAACCCAC ACAGGTGAGA AACCTTACCA CTGTGACTGG GACGGCTGTG
  301 GATGGAAATT CGCCCGCTCA GATGAACTGA CCAGGCACTA CCGTAAACAC ACGGGGCACC
  361 GCCCGTTCCA GTGCCAAAAA TGCGACCGAG CATTTTCCAG GTCGGACCAC CTCGCCTTAC
  421 ACATGAAGAG GCATTTTTAA GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT
  481 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
  541 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC
  601 AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
  661 AGCAGTTCCT GCCCCGGCTC ANGGCCAAGA ACAGATGGAA CAGCTGANTA TGGGCCAAAC
  721 AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCANGNC CAAGAACAGA TGGTCCCCAG
  781 ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GNGCCCCAAG
  841 GNNCTGAANT GANCCTGNGC CNTATTTGAA CTNACNNATC AGTTCGCTTN NCGCTTCNGN
901 NCGCGCGCNN CTGCTCCCNN NNNNNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert mid(hKLF4(H450A) mid)
Sequence file: RDB19010_B1Ecc.seq check
>D07716A7_B1Ec_8_pMXs-3LTRup_R2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTNNNCGTC GNNNNCTGTG CTGGCGGCCG CTTAAAAATG
   61 CCTCTTCNTG TGTAAGGCGA GGTGGTCCGA CCTGGAAAAT GCTCGGTCGC ATTTTTGGCA
  121 CTGGAACGGG CGGTGCCCCG TGTGTTTACG GTAGTGCCTG GTCAGTTCAT CTGAGCGGGC
  181 GAATTTCCAT CCACAGCCGT CCCAGTCACA GTGGTAAGGT TTCTCACCTG TGTGGGTTCG
  241 CAGGGCTGCC TTGAGATGGG AACTCTTTGT GTAGGTTTTG CCGCAGCCCG CGTAATCACA
  301 AGTGTGGGTG GCGGTCCTTT TCCGGGGCCA CGATCGTCTT CCCCTCTTTG GCTTGGGCTC
  361 CTCTGGCATG CAGGAACCGG GTGGCATGAG CTCTTGGTAA TGGAGCGGCG GGACTTGCGG
  421 CTGCATCTGA TCGGGCAGGA AGGATGGGTA ATTGGGCCCC GGGTGGGGAT GGAAGCCGGG
  481 AGGAAGCGGC AGGGCAGGGT GACAGTCCCT GCTGCTCAGC ACTTCCTCAA GANCCAGGGT
  541 CGGGGTAGTC CTGCTGGGGA GCTGCCGCNN CANGGGGAAN TCGTGTGCAG CCGGCCGGNG
  601 GCCATTGCTG ANANGGGGTC CAGCGCCCAA GTGGGTGCAC NAAGANANNG CCTCCTGCTT
  661 GATCTTGGGG CACGTGCGCN GCGNCCCNNC NNNGNNNNNN GNNNCCACCN CNNGNNNNNN
  721 GNCNNCNNGN NTNNNNTNNC TNANNCTNNN NGANNGANNG NNTNNNNNNN TNNNNNNNNN
781 GGNNNNNNNN NN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19010_B1Ecd.seq check
>D07716A7_B1Ec_8_gag-N_R_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGN NNNNNTTTTN NGANNAATAC AGAAACNCAG TCAGACAGAG
   61 ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG CGGCTTCGGT CCCAAACCGA
  121 AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC TCGTCTCCTA CCAGAACCAC
  181 ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA CGATCGGGAT TTTTGGACTC
  241 AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG TCTCCCAGGG TTGCGGCCGG
  301 GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT ACAGAGCTAG TTAGCTAACT
  361 AGTACCGACG CAGGCGCATA AAATCAGTCA TAGACACTAG ACAATCGGAC AGACACAGAT
  421 AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG TCCCTGGGCA GGGGTCTCCC
  481 GATCCCGGAC GAGCCCCCAA ATGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG
  541 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG
  601 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCGCCGAG TGAGGGGTTG
  661 TGGGCTCTTT TATTGAGCTC GGGGAGCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG
  721 ATTGNTTAGT TCAAATAANG CACAGGGTCA TTTCAGGTCC TTGGGGCACC CTGGAAACAT
  781 CTGATGGNTC TCTAGAAACT GCTGANGGCT GGANCGCATC TGGGGANCAT CTGTTCNTGG
  841 NCNTGANCCG GGGCANGAAC TGCTTANCNC AGATATNCNG TTNGGCCNNT ANNCAGCNGT
901 NNCATCTGNN NNNGGNCNNN NNCNNGGGNN NNANNNGNNT ANNNNNNNNN N
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19010_B1Ece.seq check
>D07716A7_B1Ec_8_lacIpro-B_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NCGNNNCNNT GNNCGNNGAA NCGGAANANC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA
  121 AATACTTAAG AAAAAAAACC AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC
  181 TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG
  241 ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG
  301 GACTGGCGCC CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC
  361 GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA
  421 GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC
  481 GCATCTGGGG ACCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA
  541 TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC
  601 TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG
  661 ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCNATGC CTTGCAAAAT GGCGTTACTT
  721 AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC NCCCTTTTTC TGGAGACTAA
  781 ATAAAATCTT TTATTTTATC GTCGACCACT GTGCTGGCGG CCGCTTAAAA ATGCCTCTTC
  841 ATGTGTAANN NGAGGTGGTC NNANCTNNAA AATGNTCNGN CGCNTTTTTT NGCACTNGAA
  901 CGGGCGGNGC CCNNNGNGNT NANNNNANNG NCNNNNNNAN NNNNTCNNAN CGGNNGAANN
961 TTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]