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pMXs-3xFLAG-KLF4(H446A) (#RDB19009)

Retroviral expression vector of human KLF4, mutant H446A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (H446A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19009 pMXs-3xFLAG-KLF4(H446A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(H446A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19009).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19009_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(H446A) 5')
Sequence file: RDB19009_B1Eca.seq check
>D07716A6_B1Ec_1_MMLV-pol_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGGC ATCGCANCTT GGNNNCACGC CGCCCACGTG AAGGCTGCCG
   61 ACCCCGGGGG TGGANCATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCCAG
  121 TGTGGTGGTA CGGGAATTCA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA
  181 CATCGACTAC AAGGATGACG ATGACAAGGC TGTCAGCGAC GCGCTGCTCC CATCTTTCTC
  241 CACGTTCGCG TCTGGCCCGG CGGGAAGGGA GAAGACACTG CGTCAAGCAG GTGCCCCGAA
  301 TAACCGCTGG CGGGAGGAGC TCTCCCACAT GAAGCGACTT CCCCCAGTGC TTCCCGGCCG
  361 CCCCTATGAC CTGGCGGCGG CGACCGTGGC CACAGACCTG GAGAGCGGCG GAGCCGGTGC
  421 GGCTTGCGGC GGTAGCAACC TGGCGCCCCT ACCTCGGAGA GAGACCGAGG AGTTCAACGA
  481 TCTCCTGGAC CTGGACTTTA TTCTCTCCAA TTCGCTGACC CATCCTCCGG AGTCAGTGGC
  541 CGCCACCGTG TCCTCGTCAG CGTCAGCCTC CTCTTCGTCG TCGCCGTCGA GCAGCGGCCC
  601 TGCCAGCGCG CCCTCCACCT GCAGCTTCAC CTATCCGATC CGGGCCGGGA ACGACCCGGG
  661 CGTGGCGCCG GGCGGCACGG GCGGANGCCT CCTCTATGGC AGGGAGTCCG CTCCCCCTCC
  721 GACGGCTCCC TTCAACCTGG CGGACATCAA CGACGTGAGC CCCTCGGGCG GCTTCGTGGC
  781 CGANCTCCTG CGGCCAGAAT TGGACCCGGT GTACATTCCG CCGCANCAGC CGCAGCCGCC
  841 AGGTGGCGGG CTGATGGGNA AGTTCGNGCT GANNGCGTCG CTGANCGCCC NNNGNANCGA
  901 GTACNGNAGN CNNNCNGNCA TCANCGNCAN NNAANGNAGC CNNNANNNNA GCCACCNGGN
961 GNNNGNNNNN NNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4(H446A) 3')
Sequence file: RDB19009_B1Ecb.seq check
>D07716A6_B1Ec_1_pMXs-3LTRup_R2_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTNN NCGTCGNNNN CTGTGCTGGC GGCCGCTTAA AAATGCCTCT
   61 TCATGTGTAA GGCGAGGTGG TCCGACCTGG AAAATGCTCG GTCGCATTTT TGGCACTGGA
  121 ACGGGCGGTG CCCCGTGTGT TTACGGTAGT GCCTGGTCAG TTCATCTGAG CGGGCGAATT
  181 TCCATCCACA GCCGTCCCAG TCACAGTGGT AAGGTTTCTC ACCTGTGTGG GTTCGCAGGT
  241 GTGCCTTGAG AGCGGAACTC TTTGTGTAGG TTTTGCCGCA GCCCGCGTAA TCACAAGTGT
  301 GGGTGGCGGT CCTTTTCCGG GGCCACGATC GTCTTCCCCT CTTTGGCTTG GGCTCCTCTG
  361 GCATGCAGGA ACCGGGTGGC ATGAGCTCTT GGTAATGGAG CGGCGGGACT TGCGGCTGCA
  421 TCTGATCGGG CAGGAAGGAT GGGTAATTGG GCCCCGGGTG GGGATGGAAG CCGGGAGGAA
  481 GCGGCAGGGC AGGGTGACAG TCCCTGCTGC TCAGCACTTC CTCAAGACCC AGGGTCGGGG
  541 TAGTCCTGCT GGGGAGCTGC CGCCCCAGGG GGAAGTCGTG TGCAGCCGGC CGGTGGCCAT
  601 TGCTGAGAGG GGGTCCAGCG CCCAAGTGGG TGCACGAAGA GACCGCCTCC TGCTTGATCT
  661 TGGGGCACGT GCGCGGCGGC CCGCCGTTGT ANGGCGCCAC CACCACCGGG TGGCTGCCGT
  721 CAGGGCTGCC TTTGCTGACG CTGATGANCG ACGGGCTGCC GTACTCGCTG CCAGGGGCGC
  781 TCAGCGACGC CTTCAGCACG AACTTGCCCA TCAGCCCGCC ACCTGGCGGC TGCGGCTGCT
  841 GCGGCGGANN GNACACCGNN NCCAATTCTG GNCGCNNNAN CTCNGNCACN AANCCGCCCN
  901 ANGGGNNCAC NNNNNNNATG NCCGNCAGGN NNNANNGNNN CCNNNNNANG GGNNNCNGNN
961 NTNCNNNNNN NNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19009_B1Ecc.seq check
>D07716A6_B1Ec_1_gag-N_R_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NTAATTTTNN GANNAATACA GAAACACAGT CAGACAGAGA
   61 CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC GGCTTCGGTC CCAAACCGAA
  121 AGCAAAAATT CAGACGGAGG CGGGAACTGT TTTAGGTTCT CGTCTCCTAC CAGAACCACA
  181 TATCCCTCCT ATTAGGGGGG TGCACCAAAG AGTCCAAAAC GATCGGGATT TTTGGACTCA
  241 GGTCGGGCCA CAAAAACGGC CCCCGAAGTC CCTGGGACGT CTCCCAGGGT TGCGGCCGGG
  301 TGTTCCGAAC TCGTCAGTTC CACCACGGGT CCGCCAGATA CAGAGCTAGT TAGCTAACTA
  361 GTACCGACGC AGGCGCATAA AATCAGTCAT AGACACTAGA CAATCGGACA GACACAGATA
  421 AGTTGCTGGC CAGCTTACCT CCCGGTGGTG GGTCGGTGGT CCCTGGGCAG GGGTCTCCCG
  481 ATCCCGGACG AGCCCCCAAA TGAAAGACCC CCGCTGACGG GTAGTCAATC ACTCAGAGGA
  541 GACCCTCCCA AGGAACAGCG AGACCACAAG TCGGATGCAA CTGCAAGAGG GTTTATTGGA
  601 TACACGGGTA CCCGGGCGAC TCAGTCAATC GGAGGACTGG CGCGCCGAGT GAGGGGTTGT
  661 GGGCTCTTTT ATTGAGCTCG GGGAGCAGAA GCGCGCGAAC AGAAGCGAGA AGCGAACTGA
  721 TTGGTTAGTT CAAATAAGGC ACAGGGTCAT TTCAGGTCCT TGGNGCACCC TGGAAACATC
  781 TGATGGNTCT CTAGAAACTG CTGANGGCTG GANCGCATCT GGGGACCATC TGTTCTTGGC
  841 CCTGNNCCNG GGCANGAACT GCTTANCACA NATATNCTGT TNGGCCCATA TTCAGCTGNN
  901 NCNTCTGNNC TTGGNCCNGA NNCGGGGNNN GNANNNGNTN ANNNNAGATN TCCNGNTTNG
961 NCNNNNNNNN NN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR,insert 3'(hKLF4(H446A) 3')
Sequence file: RDB19009_B1Ecd.seq check
>D07716A6_B1Ec_1_lacIpro-B_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCGC NNNNNNNCNG TGNGCGAGGA AGCGGAAGAN CGCCCAATAC
   61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT
  121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG
  181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG
  241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA
  301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG
  361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC
  421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC
  481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT
  541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA
  601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT
  661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT
  721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA
  781 AATAAAATCT TTTATTTTAT CGTCGACCAC TGTGCTGGCG GCCGCTTAAA AATGCCTCTT
  841 CATGTGTAAN GCGAGGTGGT CCGANCTGNA AAATGCTCGG TCGCATTTTT TGGCNNNNNA
  901 ACGGGCGGNG CCCCGNGNGT TTACNNNANT GCCNNNNNAN NTCNTNTGAN CGGGNGANNT
961 TCCATCCACA NNCNNTNCNN NNCNNNNNNN NNANNNNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]