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pMXs-3xFLAG-KLF4(K443A) (#RDB19008)

Retroviral expression vector of human KLF4, mutant K443A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (K443A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19008 pMXs-3xFLAG-KLF4(K443A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(K443A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19008).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19008_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(K443A) 5')
Sequence file: RDB19008_B1Eca.seq check
>D07716A5_B1Ec_1_MMLV-pol_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ATCGCANCTT GGNNACACGC CGCCCACGTG AAGGCTGCCG
   61 ACCCCGGGGG TGGANCATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCCAG
  121 TGTGGTGGTA CGGGAATTCA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA
  181 CATCGACTAC AAGGATGACG ATGACAAGGC TGTCAGCGAC GCGCTGCTCC CATCTTTCTC
  241 CACGTTCGCG TCTGGCCCGG CGGGAAGGGA GAAGACACTG CGTCAAGCAG GTGCCCCGAA
  301 TAACCGCTGG CGGGAGGAGC TCTCCCACAT GAAGCGACTT CCCCCAGTGC TTCCCGGCCG
  361 CCCCTATGAC CTGGCGGCGG CGACCGTGGC CACAGACCTG GAGAGCGGCG GAGCCGGTGC
  421 GGCTTGCGGC GGTAGCAACC TGGCGCCCCT ACCTCGGAGA GAGACCGAGG AGTTCAACGA
  481 TCTCCTGGAC CTGGACTTTA TTCTCTCCAA TTCGCTGACC CATCCTCCGG AGTCAGTGGC
  541 CGCCACCGTG TCCTCGTCAG CGTCAGCCTC CTCTTCGTCG TCGCCGTCGA GCAGCGGCCC
  601 TGCCAGCGCG CCCTCCACCT GCAGCTTCAC CTATCCGATC CGGGCCGGGA ACGACCCGGG
  661 CGTGGCGCCG GGCGGCACGG GCGGANGCCT CCTCTATGGC ANGGAGTCCG CTCCCCCTCC
  721 GACGGCTCCC TTCAACCTGG CGGANATCAA CGACGTGAGC CCCTCGGGCG GCTTCGTGGC
  781 CGANCTCCTG CGGCCAGAAT TGGACCCGGT GTACATTCCG CCGCAGCANC CGCANNCGCC
  841 AGGTGGCGGG CTGATGGGCA AGTTCGTGCT GAANGCGTCG CTGANCGCCC CNNNNANCGA
  901 GTACGGNAGC CCGNNNNNCA TCANCGNCAG CAAANGNANC CNNNANNNCA NNNACCCNGN
961 NGGNGGNNNN NNCNNNNNNN NGNNNGNNNN NNNNNN
//
Primer: hKLF4-mut check_R2 (Pr1009)
Region: insert 3'(hKLF4(K443A) 3'),3' LTR
Sequence file: RDB19008_B1Ecb.seq check
>D07716A5_B1Ec_1_hKLF4-mut check_R2_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNN NGGGGCCNNA TTACCCNTCC TTCCTGCCCG
   61 ATCAGATGCA GCCGCAAGTC CCGCCGCTCC ATTACCAAGA GCTCATGCCA CCCGGTTCCT
  121 GCATGCCAGA GGAGCCCAAG CCAAAGAGGG GAAGACGATC GTGGCCCCGG AAAAGGACCG
  181 CCACCCACAC TTGTGATTAC GCGGGCTGCG GCAAAACCTA CACAGCGAGT TCCCATCTCA
  241 AGGCACACCT GCGAACCCAC ACAGGTGAGA AACCTTACCA CTGTGACTGG GACGGCTGTG
  301 GATGGAAATT CGCCCGCTCA GATGAACTGA CCAGGCACTA CCGTAAACAC ACGGGGCACC
  361 GCCCGTTCCA GTGCCAAAAA TGCGACCGAG CATTTTCCAG GTCGGACCAC CTCGCCTTAC
  421 ACATGAAGAG GCATTTTTAA GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT
  481 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
  541 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC
  601 AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
  661 AGCAGTTCCT GCCCCGGCTC ANGGCCAAGA ACAGATGGAA CAGCTGANTA TGGGCCAAAC
  721 AGGATATCTG TGGTAAGCAG TTCCTGNCCC GGCTCANGGC CNAGAACAGA TGGTCCCCAG
  781 ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA NGTTTCCNGG GNGCCCCNAG
  841 GANCTGAAAT GANCCTGNGC CNTATTTGAA CNNACCNATC AGTNCGCTNN NCGCTTNNGN
901 NCNCGCGCTT CNGCTCCCNN NNNNCNNNAN ANNANNNNNN NNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert mid(hKLF4(K443A) mid)
Sequence file: RDB19008_B1Ecc.seq check
>D07716A5_B1Ec_1_pMXs-3LTRup_R2_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCGTCNN NCNCTNNNNN GGCGGCCGCT TANNNNNGCC
   61 TCTTCNTGTG TAAGGCGAGG TGGTCCGACC TGGAAAATGC TCGGTCGCAT TTTTGGCACT
  121 GGAACGGGCG GTGCCCCGTG TGTTTACGGT AGTGCCTGGT CAGTTCATCT GAGCGGGCGA
  181 ATTTCCATCC ACAGCCGTCC CAGTCACAGT GGTAAGGTTT CTCACCTGTG TGGGTTCGCA
  241 GGTGTGCCTT GAGATGGGAA CTCGCTGTGT AGGTTTTGCC GCAGCCCGCG TAATCACAAG
  301 TGTGGGTGGC GGTCCTTTTC CGGGGCCACG ATCGTCTTCC CCTCTTTGGC TTGGGCTCCT
  361 CTGGCATGCA GGAACCGGGT GGCATGAGCT CTTGGTAATG GAGCGGCGGG ACTTGCGGCT
  421 GCATCTGATC GGGCAGGAAG GATGGGTAAT TGGGCCCCGG GTGGGGATGG AAGCCGGGAG
  481 GAAGCGGCAG GGCAGGGTGA CAGTCCCTGC TGCTCAGCAC TTCCTCNNNA CCCAGGGTCG
  541 GGGTAGTCCT GCTGGGGAGC TGCCGCCCCA GGGGGAAGTC GTGTGCAGCC GGCCGGTGGC
  601 CATTGCTGAG AGGGGGTCCA GCGCCCAAGT GGGTGCACGA AGAGACCGCC TCCTGCTTGA
  661 TCTTGGGGCA CGTGCGCGGC GGCCCGCCGT TGTAGGGCGC CACCACCACC GGGTGGCTGC
  721 CGTCAGGGCT GCCTTTGCTG ACNCTGATGA CCGACGGGCT GCCGTACTCG CTGCCNGGGG
  781 CGCTCAGCGA CGCCTTCAGC ACGAACTTGC CCATCANNCN GCCACCTGGC GGCTGNGGCT
  841 GCTGCGGCGG ANNGTACACC NGGNNCNNTN NNGGCNNCNN NANCTCNNNN NNNAANNCNC
  901 NCNNANGGNN TCANGTNNNT NANGTCNNCN NGNNNNAANG NNNCCGNCGG NANGGGGANC
961 NGNANNNCNN NNNNTNNNNN NNNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19008_B1Ecd.seq check
>D07716A5_B1Ec_1_gag-N_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNTT TTNNGANNAA TACNGAAACN CAGTCAGACA
   61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC
  121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC
  181 CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA
  241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC
  301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA
  361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA
  421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT
  481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG
  541 AGGAGACCCT CCCAAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT
  601 TGGATACACG GGTACCCGGG CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG
  661 TTGTGGGCTC TTTTATTGAG CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA
  721 CTGATTGGTT AGTTCAAATA AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGANA
  781 CATCTGATGG TTCTCTAGAA ACTGCTGANG GCTGGACCGC ATCTGGGGAC CATCTGTTCT
  841 TGGCCCTGAN CCGGGGCANG AACTGCTTAC CNCAGATATC CTGTTNGGNC CATATTCAGC
  901 NGTNCCATCT NNNCNNGGCC NNNANCCGGG NNANGNNCNG NNTANNNCAG ATATCCNGNT
961 NNNNCNNTNT TCNGNNNNNN NNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19008_B1Ece.seq check
>D07716A5_B1Ec_1_lacIpro-B_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGC ANCNNNNCNG TGANCGANGA NCGGAAGANC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA
  121 AATACTTAAG AAAAAAAACC AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC
  181 TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG
  241 ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG
  301 GACTGGCGCC CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC
  361 GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA
  421 GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC
  481 GCATCTGGGG ACCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA
  541 TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC
  601 TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG
  661 ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC CTTGCAAAAT GGCGTTACTT
  721 AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC CCCCTTTTTC TGGAGACTAA
  781 ATAAAATCTT TTANTTTATC GTCGACCACT GTGCTGGCGG CCGCTTNAAA ATGCCTCTTC
  841 ATGTGTAANG NGAGGTGGTC CGACCTGNAA AATGNTCNGT CGCATTTTTN GGNACTNGAA
  901 CGGGCGGNGC CCCGNGTGTT TANNNNANTG NCTGNNCANN NNNTNNNANC GGGNNNNNTT
961 NCNTNCNCAG NCNTCCCAGT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]