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pMXs-3xFLAG-KLF4(T442A) (#RDB19007)

Retroviral expression vector of human KLF4, mutant T442A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (T442A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19007 pMXs-3xFLAG-KLF4(T442A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(T442A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19007).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19007_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(T442A) 5')
Sequence file: RDB19007_B1Eca.seq check
>D07716A4_B1Ec_1_MMLV-pol_F_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNATCGNANC TNGGNNACNC GCCGCCCACG TGAAGGCTGC
   61 CGACCCCGGG GGTGGNNCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCC
  121 AGTGTGGTGG TACGGGAATT CATGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT
  181 GACATCGACT ACAAGGATGA CGATGACAAG GCTGTCAGCG ACGCGCTGCT CCCATCTTTC
  241 TCCACGTTCG CGTCTGGCCC GGCGGGAAGG GAGAAGACAC TGCGTCAAGC AGGTGCCCCG
  301 AATAACCGCT GGCGGGAGGA GCTCTCCCAC ATGAAGCGAC TTCCCCCAGT GCTTCCCGGC
  361 CGCCCCTATG ACCTGGCGGC GGCGACCGTG GCCACAGACC TGGAGAGCGG CGGAGCCGGT
  421 GCGGCTTGCG GCGGTAGCAA CCTGGCGCCC CTACCTCGGA GAGAGACCGA GGAGTTCAAC
  481 GATCTCCTGG ACCTGGACTT TATTCTCTCC AATTCGCTGA CCCATCCTCC GGAGTCAGTG
  541 GCCGCCACCG TGTCCTCGTC AGCGTCAGCC TCCTCTTCGT CGTCGCCGTC GAGCAGCGGC
  601 CCTGCCAGCG CGCCCTCCAC CTGCAGCTTC ACCTATCCGA TCCGGGCCGG GAACGACCCG
  661 GGCGTGGCGC CGGGCGGCAC GGGCGGANGC CTCCTCTATG GCAGGGAGTC CGCTCCCCCT
  721 CCGACGGCTC CCTTCNACCT GGCGGACATC AACGACGTGA GCCCCTCGGG CGGCTTCGTG
  781 GCCGANCTCC TGCGGCCAGA ATTGGANCCG GTGTACNTTC CGCCGCANCA NCCGCAGNCG
  841 CCAGGTGGCG GGCTGANGGG NAAGTTCGTG CTGAANGNGT CNCTGANCNN CCCNNNNANC
  901 GAGNACNGNA GCCCGNNNGN CNTCAGCGTC ANCAAANGNA GCNNNGNNNN NANCCNCCCN
961 GNNGGNNGNG NNNNNNNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4(T442A) 3')
Sequence file: RDB19007_B1Ecb.seq check
>D07716A4_B1Ec_1_pMXs-3LTRup_R2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTNN NCGTCGNNNN CTNNNNNGGC GGCCGCTTAA AAATGCCTCT
   61 TCATGTGTAA GGCGAGGTGG TCCGACCTGG AAAATGCTCG GTCGCATTTT TGGCACTGGA
  121 ACGGGCGGTG CCCCGTGTGT TTACGGTAGT GCCTGGTCAG TTCATCTGAG CGGGCGAATT
  181 TCCATCCACA GCCGTCCCAG TCACAGTGGT AAGGTTTCTC ACCTGTGTGG GTTCGCAGGT
  241 GTGCCTTGAG ATGGGAACTC TTTGCGTAGG TTTTGCCGCA GCCCGCGTAA TCACAAGTGT
  301 GGGTGGCGGT CCTTTTCCGG GGCCACGATC GTCTTCCCCT CTTTGGCTTG GGCTCCTCTG
  361 GCATGCAGGA ACCGGGTGGC ATGAGCTCTT GGTAATGGAG CGGCGGGACT TGCGGCTGCA
  421 TCTGATCGGG CAGGAAGGAT GGGTAATTGG GCCCCGGGTG GGGATGGAAG CCGGGAGGAA
  481 GCGGCAGGGC AGGGTGACAG TCCCTGCTGC TCAGCACTTC CTCAAGACCC AGGGTCGGGG
  541 TAGTCCTGCT GGGGAGCTGC CGCCCCAGGG GGAAGTCGTG TGCAGCCGGC CGGTGGCCAT
  601 TGCTGAGAGG GGGTCCAGCG CCCAAGTGGG TGCACGAAGA GACCGCCTCC TGCTTGATCT
  661 TGGGGCACGT GCGCGGCGGC CCGCCGTTGT AGGGCGCCAC CACCACCGGG TGGCTGCCGT
  721 CAGGGCTGCC TTTGCTGANG CTGATGANCG ACGGGCTGCC GTACTCGCTG CCANGGGCGC
  781 TCANCGACGC NTTCNGCACG AACTTGNCCN TCAGCCCGCC ACCNNNNNGC TGCNGCTGCT
  841 GCNGCGGAAN GNACACCGGG NNNNANTCTG GNCNNNNGAN NNCGNNNNCN AAGNCNNCCN
  901 ANGGGCTCAC NNCNNNNNNG NNNNNNANNN NNNANNGNAN CCNNNNNNNG GGNNNNNNNN
961 NN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19007_B1Ecc.seq check
>D07716A4_B1Ec_1_gag-N_R_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN GNNNNNNNNN TTNNGANNAA TACAGAAACN CAGTCAGACA
   61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC
  121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC
  181 CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA
  241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC
  301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA
  361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA
  421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT
  481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG
  541 AGGAGACCCT CCCAAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT
  601 TGGATACACG GGTACCCGGG CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG
  661 TTGTGGGCTC TTTTATTGAG CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA
  721 CTGATTGGTT AGTTCAAATA AGGCACAGGG TCATTTCANG TCCTTGGGGC ACCCTGGAAA
  781 CATCTGATGG TTCTCTAGAA ACTGCTGANG GCTGGNNCGC ATCTGGGGAN NNTCTGTTCT
  841 TGGCCCTGAN CCNGGGCANG ANNTGCTTAN NACAGATATN CNGTTTGGCC CNTATTCAGC
  901 TNNNNCNTCN GNNCTNGGNC CNNNNCCNGG GNNNNNNNNG CNTANNNCAN ATATNCNNNT
961 TGGNNNNNNN NNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19007_B1Ecd.seq check
>D07716A4_B1Ec_1_lacIpro-B_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNCGNNNCNG TGANCGAGGA AGCGGAAGAN CGCCCNATAC
   61 GCAAACCGCC TCTCCCCNCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT
  121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG
  181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG
  241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA
  301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG
  361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC
  421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC
  481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT
  541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA
  601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT
  661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT
  721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA
  781 AATAAAATCT TTTATTTTAT CGTCGANNAC TGTGCTGGCG GCCGCTTAAA AATGCCTCTT
  841 CATGTGTAAN GCGAGGTGNN NNNANNNGGA AAATGNTCNG TCGNNNTTTT TGGCACTGGA
  901 ACGGGCGGNG CCCCGNGTGT TTACGGNANN GNCTGGNCAN NNCATCTGAN CGGGCGANNT
961 TNCNTCCNNN NCNNNCCNNN NCNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]