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pMXs-3xFLAG-KLF4(Y441A) (#RDB19006)

Retroviral expression vector of human KLF4, mutant Y441A.

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (Y441A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19006 pMXs-3xFLAG-KLF4(Y441A) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4(Y441A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19006).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19006_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4(Y441A)05')
Sequence file: RDB19006_B1Eca.seq check
>D07716A3_B1Ec_3_MMLV-pol_F_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN ATCGCAGCTT GGNNACACGC CGCCNNCGTG AAGGCTGCCG
   61 ACCCCGGGGG TGGACCATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCCAG
  121 TGTGGTGGTA CGGGAATTCA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA
  181 CATCGACTAC AAGGATGACG ATGACAAGGC TGTCAGCGAC GCGCTGCTCC CATCTTTCTC
  241 CACGTTCGCG TCTGGCCCGG CGGGAAGGGA GAAGACACTG CGTCAAGCAG GTGCCCCGAA
  301 TAACCGCTGG CGGGAGGAGC TCTCCCACAT GAAGCGACTT CCCCCAGTGC TTCCCGGCCG
  361 CCCCTATGAC CTGGCGGCGG CGACCGTGGC CACAGACCTG GAGAGCGGCG GAGCCGGTGC
  421 GGCTTGCGGC GGTAGCAACC TGGCGCCCCT ACCTCGGAGA GAGACCGAGG AGTTCAACGA
  481 TCTCCTGGAC CTGGACTTTA TTCTCTCCAA TTCGCTGACC CATCCTCCGG AGTCAGTGGC
  541 CGCCACCGTG TCCTCGTCAG CGTCAGCCTC CTCTTCGTCG TCGCCGTCGA GCAGCGGCCC
  601 TGCCAGCGCG CCCTCCACCT GCAGCTTCAC CTATCCGATC CGGGCCGGGA ACGACCCGGG
  661 CGTGGCGCCG GGCGGCACGG GCGGAGGCCT CCTCTATGGC AGGGAGTCCG CTCCCCCTCC
  721 GACGGCTCCC TTCAACCTGG CGGACATCAA CGACGTGAGC CCCTCGGGCG GCTTCGTGGC
  781 CGANCTCCTG CGGCCAGAAT TGGANCCGGT GTACATTCCG CCGCANCAGC CGCAGCCGCC
  841 AGGNGGCGGG CTGATGGGCA AGTNCGNGCT GAANGNGTCG CTGANCGCCC CTGGNAGCGA
  901 GNACNGNAGC CCGNCNGNCA TCNGCGTCAG CAAAGGCAGC CCNGANNNNA NNNNCCCGGN
961 GGNGNNGGCG NCNNACNACN NNNGNNNNNN NCGCACNNNN CCNNNNNNNN NN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4(Y441A) 3')
Sequence file: RDB19006_B1Ecb.seq check
>D07716A3_B1Ec_3_pMXs-3LTRup_R2_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NATCGTCGNN NNCTGTGCTG GCGGCCGCTT AAAAATGCCT
   61 CTTCATGTGT AAGGCGAGGT GGTCCGACCT GGAAAATGCT CGGTCGCATT TTTGGCACTG
  121 GAACGGGCGG TGCCCCGTGT GTTTACGGTA GTGCCTGGTC AGTTCATCTG AGCGGGCGAA
  181 TTTCCATCCA CAGCCGTCCC AGTCACAGTG GTAAGGTTTC TCACCTGTGT GGGTTCGCAG
  241 GTGTGCCTTG AGATGGGAAC TCTTTGTGGC GGTTTTGCCG CAGCCCGCGT AATCACAAGT
  301 GTGGGTGGCG GTCCTTTTCC GGGGCCACGA TCGTCTTCCC CTCTTTGGCT TGGGCTCCTC
  361 TGGCATGCAG GAACCGGGTG GCATGAGCTC TTGGTAATGG AGCGGCGGGA CTTGCGGCTG
  421 CATCTGATCG GGCAGGAAGG ATGGGTAATT GGGCCCCGGG TGGGGATGGA AGCCGGGAGG
  481 AAGCGGCAGG GCAGGGTGAC AGTCCCTGCT GCTCAGCACT TCCTCAAGAC CCAGGGTCGG
  541 GGTAGTCCTG CTGGGGAGCT GCCGCCCCAG GGGGAAGTCG TGTGCAGCCG GCCGGTGGCC
  601 ATTGCTGAGA GGGGGTCCAG CGCCCAAGTG GGTGCACGAA GAGACCGCCT CCTGCTTGAT
  661 CTTGGGGCAC GTGCGCGGCG GCCCGCCGTT GTAGGGCGCC ACCACCACCG GGTGGCTGCC
  721 GTCAGGGCTG CCTTTGCTGA CGCTGATGAC CGACGGGCTG CCGTACTCGC TGCCAGGGGC
  781 GCTCANCGAC GCCTTCAGCA CGAACTTGCC CATCAGCCCG CCACCTGGCG GCTGCGGCTG
  841 CTGCGGCGGA NTGTACACCG NGNCCNATTC TGGNCGCNNG ANCTCGGCCA CNAANCCGCC
  901 CNANGGGCTC ACGTCGTTGA TGNNCGCCAN GTTNNANGGN ANCCGNNNNA NGGGGANCNN
  961 NNTNCNTGNC NTANANNANN NNNNNNCCCG NGNCNNNCNG NNGNNNCNNC NNGNNNNNNN
1021 CNNNNNNNNN N
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19006_B1Ecc.seq check
>D07716A3_B1Ec_3_gag-N_R_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNNT TTTNNNANNN NNANNGAAAC ACAGTCAGAC
   61 AGAGACAACA CAGAACGATG CTGCAGCAGA CAAGACGCGC GGCGCGGCTT CGGTCCCAAA
  121 CCGAAAGCAA AAATTCAGAC GGAGGCGGGA ACTGTTTTAG GTTCTCGTCT CCTACCAGAA
  181 CCACATATCC CTCCTATTAG GGGGGTGCAC CAAAGAGTCC AAAACGATCG GGATTTTTGG
  241 ACTCAGGTCG GGCCACAAAA ACGGCCCCCG AAGTCCCTGG GACGTCTCCC AGGGTTGCGG
  301 CCGGGTGTTC CGAACTCGTC AGTTCCACCA CGGGTCCGCC AGATACAGAG CTAGTTAGCT
  361 AACTAGTACC GACGCAGGCG CATAAAATCA GTCATAGACA CTAGACAATC GGACAGACAC
  421 AGATAAGTTG CTGGCCAGCT TACCTCCCGG TGGTGGGTCG GTGGTCCCTG GGCAGGGGTC
  481 TCCCGATCCC GGACGAGCCC CCAAATGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
  541 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
  601 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCGC CGAGTGAGGG
  661 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
  721 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
  781 ACATCTGATG GTTCTCTAGA AACTGCTGAN GGCTGGACCG CATCTGGNGA NCATCTGTTC
  841 TTGGCCCTGA NCNNGGGCNN GAACTGCTTA CCACAGATAT CCNGNTTGGN CCATATTCNG
  901 CTGTTNCNTC TGTTCTTGGN CNNGANCCGG GGCNNGNANC TGCTNANCAC AGATATCCNG
  961 NTNNGNNCCN TANTNNNNCN GNTNNNATNN NNNNCNNGAN CNTNGNTNNN NNNNTTTNNN
1021 NNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19006_B1Ecd.seq check
>D07716A3_B1Ec_3_lacIpro-B_F_C03_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANCGNNTCN GTGAGCGAGG AANCGGAAGA GCGCCCAATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG
  121 TAAATACTTA AGAAAAAAAA CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC
  181 GCTGACGGGT AGTCAATCAC TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC
  241 GGATGCAACT GCAAGAGGGT TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG
  301 AGGACTGGCG CCCCGAGTGA GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC
  361 GCGCGAACAG AAGCGAGAAG CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT
  421 CAGGTCCTTG GGGCACCCTG GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA
  481 CCGCATCTGG GGACCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA
  541 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA
  601 ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT
  661 TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTTCCAT GCCTTGCAAA ATGGCGTTAC
  721 TTAAGCTAGC TTGCCAAACC TACAGGTGGG GTCTTTCATT CCCCCCTTTT TCTGGAGANT
  781 AAATAAAATC TTTTATTTTA TCGTCGANCA CTGTGCTGGC GGCCGCTTAA AAATGCCTCT
  841 TCATGTGTAA NGNGAGGTGG NCCGACCTGN AAAATGCTCN GNCGCATTTT NGGCACTGGA
  901 ACGGGCGGNG NCCCGNGNGT TTANGGNANT GNCNNNNCAG TTCATCTGAN CNGGNNANNT
961 TCCNTCCNCA NCNNNCCNAN NCACANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]