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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4 (#RDB19004)

Retroviral expression vector of human Kruppel like factor 4 (KLF4).

Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) tagged with 3xFLAG at N-terminus.
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19004 pMXs-3xFLAG-KLF4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-3xFLAG-KLF4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19004).

Reference section:

Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19004_B1Ecp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(3xFLAG,hKLF4 5')
Sequence file: RDB19004_B1Eca.seq check
>D07716A1_B1Ec_4_MMLV-pol_F_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNANNNGG NNTCGCANCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
  121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG
  181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT
  241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA
  301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC
  361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG
  421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG
  481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG
  541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC
  601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG
  661 GCGTGGCGCC GGGCGGCACG GGCGGAGGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC
  721 CGACGGCTCC CTTCAACCTG GCGGANATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG
  781 CCGAGCTCCT GCGGCCAGAN TTGGACCCGG TGTACATTCC GCCGCAGCAG CCGCAGCCGC
  841 CAGGNGGCGG GCTGATGGGC AANTNCGTGC TNAANGNGTC NCTGANCGCC CCTGGCAGCG
  901 ANNNNNNNAG CCCGNNNNTC ATCAGCGNCA GCNANGNCAN NCCNNANNGC NGCCNNCCGG
961 NGGTGGNGGC GNCCNNCNNN NGNNGGNNNN NNNCGCNNGN NNCCNNANNN NNNNNNN
//
Primer: pMXs-3LTRup_R2 (Pr0365)
Region: insert 3'(hKLF4 3')
Sequence file: RDB19004_B1Ecb.seq check
>D07716A1_B1Ec_4_pMXs-3LTRup_R2_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTTNNNCGN CNNNNNCTGT GCTGGCGGCC GCTTAAAAAT
   61 GCCTCTTCAT GTGTAAGGCG AGGTGGTCCG ACCTGGAAAA TGCTCGGTCG CATTTTTGGC
  121 ACTGGAACGG GCGGTGCCCC GTGTGTTTAC GGTAGTGCCT GGTCAGTTCA TCTGAGCGGG
  181 CGAATTTCCA TCCACAGCCG TCCCAGTCAC AGTGGTAAGG TTTCTCACCT GTGTGGGTTC
  241 GCAGGTGTGC CTTGAGATGG GAACTCTTTG TGTAGGTTTT GCCGCAGCCC GCGTAATCAC
  301 AAGTGTGGGT GGCGGTCCTT TTCCGGGGCC ACGATCGTCT TCCCCTCTTT GGCTTGGGCT
  361 CCTCTGGCAT GCAGGAACCG GGTGGCATGA GCTCTTGGTA ATGGAGCGGC GGGACTTGCG
  421 GCTGCATCTG ATCGGGCAGG AAGGATGGGT AATTGGGCCC CGGGTGGGGA TGGAAGCCGG
  481 GAGGAAGCGG CAGGGCAGGG TGACAGTCCC TGCTGCTCAG CACTTCCTCA AGACCCAGGG
  541 TCGGGGTAGT CCTGCTGGGG AGCTGCCGCC CCAGGGGGAA GTCGTGTGCA GCCGGCCGGT
  601 GGCCATTGCT GAGAGGGGGT CCAGCGCCCA AGTGGGTGCA CGAAGAGACC GCCTCCTGCT
  661 TGATCTTGGG GCACGTGCGC GGCGGCCCGC CGTTGTAGGG CGCCACCACC ACCGGGTGGC
  721 TGCCGTCAGG GCTGCCTTTG CTGACGCTGA TGACCGACGG GCTGCCGTAC TCGCTGCCAG
  781 GGGCGCTCAG CGACGCCTTC AGCACGAACT TGCCCATCAG CCCGCCACCT GGCGGCTGCG
  841 GCTGCTGCGG CGGANTGTAC ACCGGGNCNN ATTCTGGCCG CAGGANCTCG GCCACNAANN
  901 CGCCCNANGG GCTCACGTCN TTGANGTCCG CCNGGNTNNA NGGNNNCCNN NNNANGGGGA
  961 NCNNNNTCCC NGCCNTNNNN NANNNNTNCG NCCGNNNNNN CCGGNNCCNC NCCNNGNNNN
1021 NNNNNN
//
Primer: gag-N_R (Pr0745)
Region: MMLV psi,5' LTR
Sequence file: RDB19004_B1Ecc.seq check
>D07716A1_B1Ec_4_gag-N_R_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNNTNNTTTT NNGANNAATA CNGAAACACA GTCAGACAGA
   61 GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC GCGGCTTCGG TCCCAAACCG
  121 AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT CTCGTCTCCT ACCAGAACCA
  181 CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA ACGATCGGGA TTTTTGGACT
  241 CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC GTCTCCCAGG GTTGCGGCCG
  301 GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA TACAGAGCTA GTTAGCTAAC
  361 TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA GACAATCGGA CAGACACAGA
  421 TAAGTTGCTG GCCAGCTTAC CTCCCGGTGG TGGGTCGGTG GTCCCTGGGC AGGGGTCTCC
  481 CGATCCCGGA CGAGCCCCCA AATGAAAGAC CCCCGCTGAC GGGTAGTCAA TCACTCAGAG
  541 GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC AACTGCAAGA GGGTTTATTG
  601 GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT GGCGCGCCGA GTGAGGGGTT
  661 GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA GAAGCGAACT
  721 GATTGGTTAG TTCAAATAAG GCACAGGGTC ATTTCANGTC CTTGGGGCAC CCTGGAAACA
  781 TCTGATGGTT CTCTAGAAAC TGCTGANGGC TGGACCGCAT CTGGGGANNA TCTGTTCTTG
  841 GNCCTGANCC NGGGCAGGAA CTGCTTANCA CAGATATCCT GTTTGGNCNN TATTCNGCTG
  901 NNCCNTCTGN NCTNNNNCCT NANCCNGGNN ANGAACNGNT NANNNCAGNN ATNCNGNTNG
961 GNCNNTATTC NGCTNNNNNA TNNNNNNNNN ANNNTNNNNN NNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB19004_B1Ecd.seq check
>D07716A1_B1Ec_4_lacIpro-B_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNCGNNTNNG TGAGCGAGGA AGCGGAAGAN CGCCCAATAC
   61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT
  121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG
  181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG
  241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA
  301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG
  361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC
  421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC
  481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT
  541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA
  601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT
  661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT
  721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA
  781 AATAAAATCT TTTATTTTAT CGTCGACCAC TGTGCTGGCG GCCGCTNNNN NTGCCTCTTC
  841 ATGTGTAANG CGAGGTGGTC CGACCTNGAA AANGCTCGGT CGCATTTTTG GCACTGGAAC
  901 GGGCGGNGCC CCNNGTGTTT ANNGNANTGN CTGGNCAGTT CATCTGANCG GNNGANNTTC
  961 CNTCCNNAGC CNNCCNNNNN NCAGNGGTNN NNNTNCNCNN NTGNGTGGNN NCNNNNNNNN
1021 NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]