Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-MFG-E8/E1E2PT/Flag (#RDB18993)

Mammalian expression vector of the long form, signal sequence, E1, E2 and PT domains of mouse Mfge8 (MFG-E8) tagged with FLAG at C-terminus.

Clone info. Mammalian expression vector of the long form, signal sequence, E1, E2 and PT domains of mouse milk fat globule EGF and factor V/VIII domain containing (Mfge8, Aliases: MFG-E8) tagged with FLAG at C-terminus. See Fig.3a of Hanayama, R. et al. Nature, 417 (6885): 182-187, 2002.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pEF-BOS-EX (plasmid)
Size of vector backbone 5.3 kb
Selectable markers Ampicillin
Gene/insert name mouse Mfge8 cDNA
Depositor|Developer Nagata, Shigekazu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Hanayama, R. et al., Nature, 417 (6885): 182-187, 2002). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hanayama, R. et al., Nature, 417 (6885): 182-187, 2002)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18993 pEF-MFG-E8/E1E2PT/Flag Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEF-MFG-E8/E1E2PT/Flag was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18993).

Reference section:

Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., Nagata, S., Identification of a factor that links apoptotic cells to phagocytes. Nature 417 (6885): 182-187 (2002). PMID 12000961. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Hanayama, R., Identification of a factor that links apoptotic cells to phagocytes. Nature 417 (6885): 182-187 (2002). PMID 12000961. [link to RRC of NBRP]