Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-Tim4-Fc

Mammalian expression vector of the extracellular region of mouse Timd4 (Tim4) (aa 1-273).

Sequence (full)	RDB18988zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18988_B1Drp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,insert 5'(mTim4(aa 1-273) 5') Sequence file: RDB18988_B1Dra.seq
>D07990C7_B1Dr_3_EF1a-C_F_F04_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTT TGAGTTTGGA NCTTGGTTNN TTCTCNNGCC TCAGACAGTG    61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGAA TTCTCTAGAG ATCCCTCGAC   121 CTCGAGATCC ATCGGGCCCC CCCTCGAGGT CGACTATCAA AATGTCCAAG GGGCTTCTCC   181 TCCTCTGGCT GGTGACGGAG CTCTGGTGGC TTTATCTGAC ACCAGCTGCC TCAGAGGATA   241 CAATAATAGG GTTTTTGGGC CAGCCGGTGA CTTTGCCTTG TCATTACCTC TCGTGGTCCC   301 AGAGCCGCAA CAGTATGTGC TGGGGCAAAG GTTCATGTCC CAATTCCAAG TGCAATGCAG   361 AGCTTCTCCG TACAGATGGA ACAAGAATCA TCTCCAGGAA GTCAACAAAA TATACACTTT   421 TGGGGAAGGT CCAGTTTGGT GAAGTGTCCT TGACCATCTC AAACACCAAT CGAGGTGACA   481 GTGGGGTGTA CTGCTGCCGT ATAGAGGTGC CTGGCTGGTT CAATGATGTC AAGAAGAATG   541 TGCGCTTGGA GCTGAGGAGA GCCACAACAA CCAAAAAACC AACAACAACC ACCCGGCCAA   601 CCACCACCCC TTATGTGACC ACCACCACCC CAGAGCTGCT TCCAACAACA GTCATGACCA   661 CATCTGTTCT CCCAACCACC ACACCACCCC AGACACTAGC CACCACTGCC TTCAGTACAG   721 CAGTGACCAC GTGCCCCTCA ACAACACCTG GCTCCTTCTC ACAAGAAACC ACAAAAGGGT   781 CCGCCTTCAC TACAGAATCA GAAACTCTGC CTGCATCCNA TCACTCTCAA AGAANCATGA   841 TGACCATATC TACAGACATA GCCGTANTCN NGNCCACAGG CTCTAANCCN GGGANTCTCC   901 CNTCCACTTN NCAGCTGANG ANNCAGAAAA CAACATTAAC NACAAGTGAN TCTTTGCANA   961 ANANNANTAA NNCACATCNN ANTAAGCCNA NATNNAATTN CNNGNNGCCN NGGGGNNNNN 1021 NNNANNNNNA NNNNNNNNNN //
Primer: hIgG-genome_R1 (Pr1105) Region: insert 3'(splice donor site,mTim4(aa 1-273) 3') Sequence file: RDB18988_B1Drb.seq
>D07990C7_B1Dr_3_hlgG-genome_R1_H04_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTGANCC CNGCACCTGC CCCTTTGTGT    61 GCAGGGCCTG GGTTAGGGGC ACCTAGCCTG TGCCTGCCCA GAGCCTGGGG AAAAAGCCAG   121 AAGACCCTCT CCCTGAGCAT GAGTGGGGCG GGCAGAGGCC TCCGGGTGAA GAGGCAGACG   181 GGGCCTGCCT TGCTGCCCTG GACTGGGACT GCATAGCCGG GATGCGTCCA GGCANGATAG   241 AACTAGTGGA TCCCCCGGGC TGCAGGAATT CGATATCGGC TTACTCTGAT GTGATTTAGT   301 TGTCTTCTGC AAAGACTCAC TTGTTGTTAA TGTTGTTTTC TGTGTCGTCA GCTGTGAAGT   361 GGATGGGAGA ATCCCAGGGT TAGAGCCTGT GGGCCTGAGT ACGGCTATGT CTGTAGATAT   421 GGTCATCATG CTTCTTTGAG AGTGATTGGA TGCAGGCAGA GTTTCTGATT CTGTAGTGAA   481 GGCGGACCCT TTTGTGGTTT CTTGTGAGAA GGAGCCAGGT GTTGTTGAGG GGCACGTGGT   541 CACTGCTGTA CTGAAGGCAG TGGTGGCTAG TGTCTGGGGT GGTGTGGTGG TTGGGAGAAC   601 AGATGTGGTC ATGACTGTTG TTGGAAGCAG CTCTGGGGTG GTGGTGGTCA CATAAGGGGT   661 GGTGGTTGGC CGGGTGGTTG TTGTTGGTTT TTTGGTTGTT GTGGCTCTCC TCAGCTCCAA   721 GCGCACATTC TTCTTGACAT CNNTGNAANC ANCCAGGCAC CTCTATACNG CANCAGTACN   781 CCCCACTGTN NNCTCGATTG GTGNTTGANA NGGTCAAGGA NACTTCNNCN AACTGNACCT   841 TCCCCAAAGT GNATATTTTG TTGANTNNCT GGAGATGATT CTTGTNNCAT CTGTNNGGAN   901 AAGCTCTGCN TTGCACTTGN ANTGGGANNT NNANCTTTNN CCNNGCANAT ACTGTTGNNN   961 CTCTGGNNNN NNNANAGNNN TGACNNGNAA ANTCNCNGGC TGNNNNAAAA CCCTATNNNT 1021 GNATCNNNNN ANGNNNNTGN NGNNNNANAA GNNNNNNNNN N //
Primer: hGCSF_R (Pr0031) Region: hG-CSF 3'UTR+pA,IgG Fc-region Sequence file: RDB18988_B1Drc.seq
>D07990C7_B1Dr_3_hGCSF_R_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNTCCN NGGTGGGGNN CCTCACTCTA GAGTCGCGGC    61 CGCTCTAGGG CAGTGGTGGG TGCTTTATTT CCATGCTGGG TGCCTGGGAA GTATGTAGAC   121 GGGGTACGTG CCAAGCATCC TCGTGCGACC GCGAGAGCCC GGGGAGCGGG GGCTTGCCGG   181 CCGTCGCACT CATTTACCCG GGGACAGGGA GAGGCTCTTC TGCGTGTAGT GGTTGTGCAG   241 AGCCTCATGC ATCACGGAGC ATGAGAAGAC GTTCCCCTGC TGCCACCTGC TCTTGTCCAC   301 GGTGAGCTTG CTATAGAGGA AGAAGGAGCC GTCGGAGTCC AGCACGGGAG GCGTGGTCTT   361 GTAGTTGTTC TCCGGCTGCC CATTGCTCTC CCACTCCACG GCGATGTCGC TGGGATAGAA   421 GCCTTTGACC AGGCAGGTCA GGCTGACCTG GTTCTTGGTC ATCTCCTCCC GGGATGGGGG   481 CAGGGTGTAC ACCTGTGGTT CTCGGGGCTG CCCTGTAGGG ACAGAGGTTG GTACAGCGGT   541 CACTCTCAGG GCAGAGGGTG GGCCGAGCCG GCCTCTGTCC ATGTGGCCCT CGCACCCCAC   601 GGGTCCCACC TTTGGCTTTG GAGATGGTTT TCTCGATGGG GGCTGGGAGG GCTTTGTTGG   661 AGACCTTGCA CTTGTACTCC TTGCCATTCA GCCAGTCCTG GTGCAGGACG GTGAGGACGC   721 TGACCACACG GTACGTGCTG TTGTACTGCT CCTCCCGCGG CTTTGTCTTG GCATTATGCA   781 CCTCCACGCC GTCCACGTAC CAGTTGAACT TGACCTCAGG GTCTTCGTGG CTCACGTCCA   841 CCACCACGCN TGTGANCTCA NGGGTCCGGG ANATCATGAG GGTGTCCTTG GGTTTTGGGG   901 GGAAGANGAA GANTGANNGN CCCCCNAGGA NTTCNNNGCT GANGAANANA TGGANGTGGA   961 CGNGTCANCA CCCGGNTGGG GNNTGNCCCN NNNNGNNNNC TANTCTNGGG CNNNNGNNCN  1021 NNCNTGNNNN NNNNGNNNAN NNCNNNNNTN NNNNANCNTG GGNNCNGNNG GNNNNNNNNN 1081 N //
Primer: Fc-CH2M_R (Pr1251) Region: IgG Fc-region,insert 3'(splice donor site, mTim4(aa 1-273) 3') Sequence file: RDB18988_B1Drd.seq
>18988_18988_B1Dr_3_Fc-CH2M_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNGCTGN NNNCNCGGTA CGTGCTGTTG TACTGCTCCT    61 CCCGCGGCTT TGTCTTGGCA TTATGCACCT CCACGCCGTC CACGTACCAG TTGAACTTGA   121 CCTCAGGGTC TTCGTGGCTC ACGTCCACCA CCACGCATGT GACCTCAGGG GTCCGGGAGA   181 TCATGAGGGT GTCCTTGGGT TTTGGGGGGA AGAGGAAGAC TGACGGTCCC CCCAGGAGTT   241 CAGGTGCTGA GGAAGAGATG GAGGTGGACG TGTCAGCACC CGGCTGGGGC CTGTCCCTGG   301 ATGCAGGCTA CTCTAGGGCA CCTGTCCCGC CTTGAGCTGG AGGGCGAGGC CTGGGCTGGC   361 TTACCTGGGC ACGGTGGGCA TGTGTGAGTT TTGTCACAAG ATTTGGGCTC TGCAGAGAGA   421 AGATTGGGAG TTACTGGAAT CTGGGAGGAG AGAAGGTGTC CGAGCTGAGG GAGTGGAGAG   481 TTTGGCCTTT GGGGTGGGCT TAGGTCAGGG GCAGGGTCCT CCCGGATATG GCTCTTGGCA   541 GGTCTGAGCC CAGCACCTGC CCCTTTGTGT GCAGGGCCTG GGTTAGGGGC ACCTAGCCTG   601 TGCCTGCCCA GAGCCTGGGG AAAAAGCCAG AAGACCCTCT CCCTGAGCAT GAGTGGGGCG   661 GGCAGAGGCC TCCGGGTGAA GAGGCAGACG GGGCCTGCCT TGCTGCCCTG GACTGGGACT   721 GCATAGCCGG GATGCGTCCA GGCAGGATAG AACTAGTGGA TCCCCCGGGC TGCAGGAATT   781 CGATATCGGC TTACTCTGAT GTGATTTAGT TGTCTTCTGC NAAGACTCAC TTGTTGTTAA   841 TGNTGTTTTC TGTGTCGTCA GCTGTGAAGT GNATGGGAGA ATCCCNGGGN TAGAGCCTGT   901 GGGCCTGANT ACNGNTATGT CTGTAGATAT GGNCATCNTG CNTNCNTTGA NAGTGNNNGG   961 NTGNNNGNNN ANTTTCTGAT TCTGTANTNN NGGNNGNANN NTTTTGTGNT TTCTTGNNAN 1021 ANNNANCCNN GNNNNNNNNA GGGGNNNNNN NNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,SV40 pro_ori,lacO,lac pro Sequence file: RDB18988_B1Dre.seq
>18988_18988_B1Dr_3_EF1a-N_R_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNG NNGNNGTGCG CTCTGCCCNC TGACGGGCAC CGGAGCCNAT    61 TCCCACTCCT TTCAAGACCT AGAAGGTCCA TTAGCTGCAA AGATTCCTCT CTGTTTAAAA   121 CTTTATCCAT CTTTGCAAAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC   181 TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT   241 AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA   301 GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT   361 GCTGGGGAGG GAATTAAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG   421 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG   481 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC   541 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT   601 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT   661 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA   721 TAACGCAGGA AAGAACATGT GAGCAAAAGG NCAGCAAAAG GNCAGGAANC GTAAAAAGGC   781 CGCGTTGCTG GCGTTTTTCC NTAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG   841 CTCAAGTCAN ANGTGNNGAA ACCCGACNGG ANTATAAAGA TACCAGGCGT TTCCCCCTGN   901 AANCTNCCTC GNGCGCTCTN CTGTTNCNAN CCTGNCGCTT ANNGGANANC TGTCCGCCTT   961 TNNCCCNNNN GGNANCGNNN NNCTTTCTCN NANCTCNNGC NNNNNNNTCT NANTTNNGNN 1021 GNNNNNCNNN NGNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

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