Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-Tim3-Fc

Mammalian expression vector of the extracellular region of mouse Havcr2 (Tim3) (aa 1-174).

Sequence (full)	RDB18987zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18987_B1Drp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro, insert(mTim3(aa1-174),splice donor site),IgG Fc-region Sequence file: RDB18987_B1Dra.seq
>D07990C6_B1Dr_1_EF1a-C_F_A05_02_ABI24.ab1     1 NNNNNNNNNN NNNTNNNCCN TTTTTNANTT TGNNNCTTGG TTNNTTCTCN AGCCTCAGAC    61 AGTGGTTCAA AGTTTTTTTC TTCCNTTTCA GGTGTCGTGA GGAATTCTCT AGAGATCCCT   121 CGACCTCGAG ATCCATCGGG CCCCCCCTCG AGGTCGACCA AGTACTCATG TTTTCAGGTC   181 TTACCCTCAA CTGTGTCCTG CTGCTGCTGC AACTACTACT TGCAAGGTCA TTGGAAAATG   241 CTTATGTGTT TGAGGTTGGT AAGAATGCCT ATCTGCCCTG CAGTTACACT CTATCTACAC   301 CTGGGGCACT TGTGCCTATG TGCTGGGGCA AGGGATTCTG TCCTTGGTCA CAGTGTACCA   361 ACGAGTTGCT CAGAACTGAT GAAAGAAATG TGACATATCA GAAATCCAGC AGATACCAGC   421 TAAAGGGCGA TCTCAACAAA GGAGACGTGT CTCTGATCAT AAAGAATGTG ACTCTGGATG   481 ACCATGGGAC CTACTGCTGC AGGATACAGT TCCCTGGTCT TATGAATGAT AAAAAATTAG   541 AACTGAAATT AGACATCAAA GCAGCCAAGG TCACTCCAGC TCAGACTGCC CATGGGGACT   601 CTACTACAGC TTCTCCAAGA ACCCTAACCA CGGAGAGAAA TGGTTCAGAG ACACAGACAC   661 TGGTGACCCT CCATAATAAC AATGGAACAA GTAAGAAGAT ATCGAATTCC TGCAGCCCGG   721 GGGATCCACT AGTTCTATCC TGCCTGGACG CATCCCGGCT ATGCAGTCCC AGTCCAGGGC   781 AGCAAGGCAG GCCCCGTCTG CCTCTTCACC CGGANGCCTC TGCCCGCCCC ACTCATGCTC   841 ANGNANANGG TCTTCTGGCT TTTTNCCNAN GCTCTGGGCA NGCACAGGNT ANGNGCCCCT   901 AACCCANGCC CNGCACACAA AGGGGCANGN GCTGGGCTCA NANCTGNCNA GANNCNTATC   961 CNGNNNGNNC CTGCCCCTGA CCTAANCCCN CCCCAAANNN NNAACNNTCC ANNCCNNCNN 1021 NNNNNNNN //
Primer: hIgG-genome_R1 (Pr1105) Region: IgG Fc-region,insert 3'(splice donor site,mTim3(aa1-174) 3') Sequence file: RDB18987_B1Drb.seq
>D07990C6_B1Dr_1_hlgG-genome_R1_C05_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTNTTGNNNG GTCTGANCCN NGCACCTGCC CCTTTGTGTG    61 CAGGGCCTGG GTTAGGGGCA CCTAGCCTGT GCCTGCCCAG AGCCTGGGGA AAAAGCCAGA   121 AGACCCTCTC CCTGAGCATG AGTGGGGCGG GCAGAGGCCT CCGGGTGAAG AGGCAGACGG   181 GGCCTGCCTT GCTGCCCTGG ACTGGGACTG CATAGCCGGG ATGCGTCCAG GCAGGATAGA   241 ACTAGTGGAT CCCCCGGGCT GCAGGAATTC GATATCTTCT TACTTGTTCC ATTGTTATTA   301 TGGAGGGTCA CCAGTGTCTG TGTCTCTGAA CCATTTCTCT CCGTGGTTAG GGTTCTTGGA   361 GAAGCTGTAG TAGAGTCCCC ATGGGCAGTC TGAGCTGGAG TGACCTTGGC TGCTTTGATG   421 TCTAATTTCA GTTCTAATTT TTTATCATTC ATAAGACCAG GGAACTGTAT CCTGCAGCAG   481 TAGGTCCCAT GGTCATCCNG AGTCACATTC TTTATGATCA GANACACGTC TCCTTTGTTG   541 AGATCGCNCT TTANCTGGTA TCTGCTGGAN TTCTNATATG TCACATTTCT TTCNTCAGTT 601 CTGANCNACT CGTTGGTACA CTGNGANNNN NNNNNN //
Primer: hGCSF_R (Pr0031) Region: IgG Fc-region Sequence file: RDB18987_B1Drc.seq
>D07990C6_B1Dr_1_hGCSF_R_B05_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GTGGGGNNCC TCNNTCTAGA GTCGCGGCCG    61 CTCTAGGGCA GTGGTGGGTG CTTTATTTCC ATGCTGGGTG CCTGGGAAGT ATGTAGACGG   121 GGTACGTGCC AAGCATCCTC GTGCGACCGC GAGAGCCCGG GGAGCGGGGG CTTGCCGGCC   181 GTCGCACTCA TTTACCCGGG GACAGGGAGA GGCTCTTCTG CGTGTAGTGG TTGTGCAGAG   241 CCTCATGCAT CACGGAGCAT GAGAAGACGT TCCCCTGCTG CCACCTGCTC TTGTCCACGG   301 TGAGCTTGCT ATAGAGGAAG AAGGAGCCGT CGGAGTCCAG CACGGGAGGC GTGGTCTTGT   361 AGTTGTTCTC CGGCTGCCCA TTGCTCTCCC ACTCCACGGC GATGTCGCTG GGATAGAAGC   421 CTTTGACCAG GCAGGTCAGG CTGACCTGGT TCTTGGTCAT CTCCTCCCGG GATGGGGGCA   481 GGGTGTACAC CTGTGGTTCT CGGGGCTGCC CTGTAGGGAC AGAGGTTGGT ACAGCGGTCA   541 CTCTCAGGGC AGAGGGTGGG CCGAGCCGGC CTCTGTCCAT GTGGCCCTCG CACCCCACGG   601 GTCCCACCTT TGGCTTTGGA GATGGTTTTC TCGATGGGGG CTGGGAGGGC TTTGTTGGAG   661 ACCTTGCACT TGTACTCCTT GCCATTCAGC CAGTCCTGGT GCAGGACGGT GAGGACGCTG   721 ACCACACGGT ACGTGCTGTT GTACTGCTCC TCCCGCGGCT TTGTCTTGNC ATTATGCACC   781 TCCACGCCGT CCACGTACCA GTTGAACTTG ACCTCAGGGT CTTCGTGGCT CACGTCCACC   841 ACCNCGCATG TGACCTCNNG GGNNCGGGAN ATCATGANGG TGNNCTTGGG TTTTGGGGGG   901 AAGAGGAAGN NTGACGGTCC CCCCNNNANT TCANNNGCTN ANNANNANAN GGANGTGGAN   961 GTGNCANCNC CCGGNNGGGG NCTGNNCCNN NNNGCNNNNT ANTCNNNNGG NANCNNNNCN 1021 NNCNTNGANC NNNNNNNNNN //
Primer: Fc-CH2M_R (Pr1251) Region: IgG Fc-region Sequence file: RDB18987_B1Drd.seq
>D07990C6_B1Dr_1_Fc-CH2M_R_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG NNGCTGNNNN CNCGGNNNCG TGCTGTTGTA    61 CTGCTCCTCC CGCGGCTTTG TCTTGGCATT ATGCACCTCC ACGCCGTCCA CGTACCAGTT   121 GAACTTGACC TCAGGGTCTT CGTGGCTCAC GTCCACCACC ACGCATGTGA CCTCAGGGGT   181 CCGGGAGATC ATGAGGGTGT CCTTGGGTTT TGGGGGGAAG AGGAAGACTG ACGGTCCCCC   241 CAGGAGTTCA GGTGCTGAGG AAGAGATGGA GGTGGACGTG TCAGCACCCG GCTGGGGCCT   301 GTCCCTGGAT GCAGGCTACT CTAGGGCACC TGTCCCGCCT TGAGCTGGAG GGCGAGGCCT   361 GGGCTGGCTT ACCTGGGCAC GGTGGGCATG TGTGAGTTTT GTCACAAGAT TTGGGCTCTG   421 CAGAGAGAAG ATTGGGAGTT ACTGGAATCT GGGAGGAGAG AAGGTGTCCG AGCTGAGGGA   481 GTGGAGAGTT TGGCCTTTGG GGTGGGCTTA GGTCAGGGGC AGGGTCCTCC CGGATATGGC   541 TCTTGGCAGG TCTGAGCCCA GCACCTGCCC CTTTGTGTGC AGGGCCTGGG TTAGGGGCAC   601 CTAGCCTGTG CCTGCCCAGA GCCTGGGGAA AAAGCCAGAA GACCCTCTCC CTGAGNNNNN   661 NTGGGGCGGG CAGAGGCCTC CGGGTGAAGA GGCAGACGGG GCCTGNNNNG CTGCCCTNNN   721 NNGGGACNNN NNNGCCGGGA TGCGTCCAGN NNNGATAGAA NNTANTGGAT CCCCCNGGCT   781 GCAGGAATTC NATATNNTTC TTACTTGTNN NNNNGNTNAT TATGGAGGGG TCANCNNNNG   841 TCTGTGTCTC TGAACCCATT TCTCTNCCGT GGTTAGGGNN NNNNGGAGAA NNCTGTNNNA 901 NANTCCCCAN NNGGCAGTCT NAANCNNNNN NNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,SV40 pro_ori,lacO,lac pro Sequence file: RDB18987_B1Dre.seq
>D07990C6_B1Dr_1_EF1a-N_R_D01_04_ABI08.ab1     1 NNNNNNNNNN NNNGNNNNNN NNNGGGNGNN GTGCGCTCTG CCNNCTGACG GGCACCGGAN    61 CCAATTCCCA CTCCTTTCAA GACCTAGAAG GTCCATTAGC TGCAAAGATT CCTCTCTGTT   121 TAAAACTTTA TCCATCTTTG CAAAGCTTTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC   181 CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA   241 AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG   301 GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC   361 TGCCTGCTGG GGAGGGAATT AAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA   421 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC   481 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC   541 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC   601 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT   661 CGGCTGCGGC GAGCGGTATC ANCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA   721 GGGGATAACN CANGAAAAGA ACATGTGAGC AAAANGNCAG CAAAAGGNCA GGANCCGTAA   781 AAAGGCCNCG TTGCTGGCGT TTTTCCATAG GCTCCNCCCC CCTGACGAGC ATCACAAAAA   841 TCNACGCTCA NGTCAGANGT GGNNNANCCN NNCNNGACTA TAAAGANNNN AGGCGTTTNC   901 CCCTGGNANC TCCCTCNTGN GCTCTCCNGT NNNNNNCCCT GCCGCTTNNC NGATNNCNNG   961 NNCNNNNNTC TCCCNTNNGG NAGCGTNGNN NNTTTCTCNT AGCTNANNCT GTNNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.10.05

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