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pMXs-puro-Tim2-EGFP

Retroviral expression vector of mouse Timd2 (Tim2) tagged with EGFP at C-terminus.

Catalog number RDB18983
Resource name pMXs-puro-Tim2-EGFP
Clone info. Retroviral expression vector of mouse T cell immunoglobulin and mucin domain containing 2 (Timd2, Aliases: Tim2) tagged with EGFP at C-terminus.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pMXs-puro, T. Kitamura, et al. Exp. Hematol. 31, 1007-1014 (2003). (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Growth remarks Recommended E. coli for plamid amplication: DH10B.
Gene/insert name mouse Timd2 cDNA
Depositor|Developer Nagata, Shigekazu |
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External Database
mouse Timd2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
          Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
          付加的提供条件 pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18983 pMXs-puro-Tim2-EGFP Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Miyanishi, M., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Miyanishi, M., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135.

          2022.07.13

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