RDB18979 - pMXs-puro-Tim2

Catalog number	RDB18979
Resource name	pMXs-puro-Tim2
Clone info.	Retroviral expression vector of mouse T cell immunoglobulin and mucin domain containing 2 (Timd2, Aliases: Tim2).
Vector backbone	pMXs-puro, T. Kitamura, et al. Exp. Hematol. 31, 1007-1014 (2003). (plasmid)
Selectable markers	Ampicillin (E. coli), puromycin (mammalian cell)
Growth remarks	Recommended E. coli for plamid amplication: DH10B.
Gene/insert name	mouse Timd2 cDNA
Depositor\|Developer	Nagata, Shigekazu \|

Other clones in our bank	mouse Timd2 (NCBI Gene 171284) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions for distribution	Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

Distribution information (domestic users)

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
付加的提供条件	pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

References and tips

Sequence information

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB18979	pMXs-puro-Tim2	Under QC test. Please contact us.	DNA solution

original	Miyanishi, M., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135.

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.