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pEF-Fc

Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region.

Catalog number RDB18974
Resource name pEF-Fc
Clone info. Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region. Please see Suda, T. and Nagata., J. Exp. Med. 179 (3): 873-878, 1994. Transfection of this plasmid into mammalian cells (293 T or COS7 cells) produces a soluble chimeric protein consisting of the extracellular region of membrane protein fused to human IgG Fc region.
Vector backbone pEF-BOS (RDB07939) (plasmid)
Selectable markers Ampicillin
Gene/insert name human IgG1
Depositor|Developer Nagata, Shigekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18974 pEF-Fc Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Suda, T., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Suda, T., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364.

2021.08.13

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