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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Fc

Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region.

Catalog number RDB18974
Resource name pEF-Fc
Clone info. Expression vector of secreted recombinant chimeric protein for the extracellular region of a membrane protein with human Immunoglobulin heavy chain region. Please see Suda, T. and Nagata., J. Exp. Med. 179 (3): 873-878, 1994. Transfection of this plasmid into mammalian cells (293 T or COS7 cells) produces a soluble chimeric protein consisting of the extracellular region of membrane protein fused to human IgG Fc region.
Comment This clone contains a genomic DNA fragment corresponding to the 105743170nt to 105741000nt of NC_000014.9. Known differences (at least) : C to T substitution at 105742694nt (in intron). ; T to G substitution at 105741749nt (Asp to Glu). ; C to A substitution at 105741745nt (Leu to Met). ; C to T substitution at 105741596nt (Tyr, synonym). ; A to G substitution at 105741506nt (Thr, synonym). ; T to C substitution at 105741485nt (Ser, synonym). ; C to G substitution at 105741468nt (in intron). ; A to G substitution at 105741443nt (in intron). ; G to C substituiton at 105741434nt (in intron). ; G to A substitution at 105741427nt (in intron). ; G to C substitution at 105741401nt (in intron) ; G to C substitution at 105741359nt (in intron). ; G to A substitution at 105741357nt (in intron). ; T to G substitution at 105741354nt (in intron).
Vector backbone pEF-BOS (RDB07939) (plasmid)
Selectable markers Ampicillin
Gene/insert name human IgG1
Depositor|Developer Nagata, Shigekazu |
Other clones in our bank

External Database
human IgG1

            Reference sequence
              
             
            Sequence (full) RDB18974zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Suda, T. and Nagata, S., J. Exp. Med. 179 (3): 873-878, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18974 pEF-Fc DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Suda, T., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364.

            Further references such as user reports and related articles (go to bottom)

            Featured content

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18974_B1Dkp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F
            Region: EF-1 alpha pro,MCS,insert 5'
            Sequence file: RDB18974_B1Dka.seq check
            >D07990B5_B1Dk_1_EF1a-C_F_A08_02_ABI24.ab1
                1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTNNTTCT CNAGCCTCAG ACAGTGGTTC
               61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGATCC CTCGACCTCG
              121 AGATCCATCG GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCTG
              181 CAGCCCGGGG GATCCACTAG TTCTATCCTG CCTGGACGCA TCCCGGCTAT GCAGTCCCAG
              241 TCCAGGGCAG CAAGGCAGGC CCCGTCTGCC TCTTCACCCG GAGGCCTCTG CCCGCCCCAC
              301 TCATGCTCAG GGAGAGGGTC TTCTGGCTTT TTCCCCAGGC TCTGGGCAGG CACAGGCTAG
              361 GTGCCCCTAA CCCAGGCCCT GCACACAAAG GGGCAGGTGC TGGGCTCAGA CCTGCCAAGA
              421 GCCATATCCG GGAGGACCCT GCCCCTGACC TAAGCCCACC CCAAAGGCCA AACTCTCCAC
              481 TCCCTCAGCT CGGACACCTT CTCTCCTCCC AGATTCCAGT AACTCCCAAT CTTCTCTCTG
              541 CAGAGCCCAA ATCTTGTGAC AAAACTCACA CATGCCCACC GTGCCCAGGT AAGCCAGCCC
              601 AGGCCTCGCC CTCCAGCTCA AGGCGGGACA GGTGCCCTAG AGTAGCCTGC ATCCAGGGAC
              661 AGGCCCCAGC CGGGTGCTGA CACGTCCACC TCCATCTCTT CCTCAGCACC TGAACTCCTG
              721 GGGGGANCGT CAGTCTTCCT CTTCCCCCCA AAACCCAAGG ACACCCTCAT GATCTCCCGG
              781 ACCCCTGAGG TCACATGCGT GGTGNTGGAN GTGAGCCACG AANANCCTGA NGTCAAGTTC
              841 AACTGGTACG TGGANGGCGN GGANGTGCAT AATGNCNAGA NAAAGCCGCN GGGANGANCA
              901 NTACAACAGC NCGNACCGTG TGGNCAGCGN NNNNANNNTN NTGCNCNNNN ANNNNNTGNA
            961 NNGNCANNGN NTANNNNNNN NANNNNCTNN NACNAANNNN NNNNNN
            //
            Primer: hGCSF_R
            Region: hG-CSF 3'UTR+pA,insert 3'
            Sequence file: RDB18974_B1Dkb.seq check
            >D07990B5_B1Dk_1_hGCSF_R_D08_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN GTNCNNGGTG GGGNNCCTCN CTCTAGAGTC GCGGCCGCTC
               61 TAGGGCAGTG GTGGGTGCTT TATTTCCATG CTGGGTGCCT GGGAAGTATG TAGACGGGGT
              121 ACGTGCCAAG CATCCTCGTG CGACCGCGAG AGCCCGGGGA GCGGGGGCTT GCCGGCCGTC
              181 GCACTCATTT ACCCGGGGAC AGGGAGAGGC TCTTCTGCGT GTAGTGGTTG TGCAGAGCCT
              241 CATGCATCAC GGAGCATGAG AAGACGTTCC CCTGCTGCCA CCTGCTCTTG TCCACGGTGA
              301 GCTTGCTATA GAGGAAGAAG GAGCCGTCGG AGTCCAGCAC GGGAGGCGTG GTCTTGTAGT
              361 TGTTCTCCGG CTGCCCATTG CTCTCCCACT CCACGGCGAT GTCGCTGGGA TAGAAGCCTT
              421 TGACCAGGCA GGTCAGGCTG ACCTGGTTCT TGGTCATCTC CTCCCGGGAT GGGGGCAGGG
              481 TGTACACCTG TGGTTCTCGG GGCTGCCCTG TAGGGACAGA GGTTGGTACA GCGGTCACTC
              541 TCAGGGCAGA GGGTGGGCCG AGCCGGCCTC TGTCCATGTG GCCCTCGCAC CCCACGGGTC
              601 CCACCTTTGG CTTTGGAGAT GGTTTTCTCG ATGGGGGCTG GGAGGGCTTT GTTGGAGACC
              661 TTGCACTTGT ACTCCTTGCC ATTCAGCCAG TCCTGGTGCA GGACGGTGAG GACGCTGACC
              721 ACACGGTACG TGCTGTTGTA CTGCTCCTCC CGCGGCTTTG TCTTGGCATT ATGCACCTCC
              781 ACGCCGTCCA CGTACCAGTT GAACTTGACC TCANGGTCTT CGTGGCTCAC GTCCACCACC
              841 ACGCATGTGN CCTCANGGGT CCGGGANATC ATGANGGNGT CCTTNGGTTT TGGGGGGGAA
              901 GAGGAAGANT GANNGNCCCC CCANGANTTC ANGTGCTGAN GAANANNNGN NNGTGGNNGN
            961 NNCANCNCCC NGCTGGGNNN TNNNCNNNNN NGCNNNNNAN NNNNNGNNNN NNNNNN
            //
            Primer: Fc-CH2M_R
            Region: MCS,insert 5'
            Sequence file: RDB18974_B1Dkc.seq check
            >18974_18974_B2IsF_1_Fc-CH2M_R_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGG NGNNGNNGCT GNCCNCACGG TACGTGCTGT TGTACTGCTC
               61 CTCCCGCGGC TTTGTCTTGG CATTATGCAC CTCCACGCCG TCCACGTACC AGTTGAACTT
              121 GACCTCAGGG TCTTCGTGGC TCACGTCCAC CACCACGCAT GTGACCTCAG GGGTCCGGGA
              181 GATCATGAGG GTGTCCTTGG GTTTTGGGGG GAAGAGGAAG ACTGACGGTC CCCCCAGGAG
              241 TTCAGGTGCT GAGGAAGAGA TGGAGGTGGA CGTGTCAGCA CCCGGCTGGG GCCTGTCCCT
              301 GGATGCAGGC TACTCTAGGG CACCTGTCCC GCCTTGAGCT GGAGGGCGAG GCCTGGGCTG
              361 GCTTACCTGG GCACGGTGGG CATGTGTGAG TTTTGTCACA AGATTTGGGC TCTGCAGAGA
              421 GAAGATTGGG AGTTACTGGA ATCTGGGAGG AGAGAAGGTG TCCGAGCTGA GGGAGTGGAG
              481 AGTTTGGCCT TTGGGGTGGG CTTAGGTCAG GGGCAGGGTC CTCCCGGATA TGGCTCTTGG
              541 CAGGTCTGAG CCCAGCACCT GCCCCTTTGT GTGCAGGGCC TGGGTTAGGG GCACCTAGCC
              601 TGTGCCTGCC CAGAGCCTGG GGAAAAAGCC AGAAGACCCT CTCCCTGAGC ATGAGTGGGG
              661 CGGGCAGAGG CCTCCGGGTG AAGAGGCAGA CGGGGCCTGC CTTGCTGCCC TGGACTGGGA
              721 CTGCATAGCC GGGATGCGTC CAGGCAGGAT AGAACTAGTG GATCCCCCGG GCTGCANGAA
              781 TTCGATATCA AGCTTATCGA TACCGTCGAC CTCGAGGGGG GGCCCGATGG ATCTCGAGGT
              841 CGAGGGATCT CTAGAGAATT CCTCACGACA CCTGAAATGG AAGAAAAAAA CTTTGAACCA
              901 CTGTCTGANN NTTGAGAATG AACCNAGATN CAAACTCAAA AAGGGNNNNT CCNNNNAGAA
            961 TTACNTCNNG TGNNNNGCTG GNNTNNCTTC NNNNTNNNCC NNNNNNNNNN
            //
            Primer: EF1a-N_R
            Region: EF-1 alpha pro,SV40 pro_ori
            Sequence file: RDB18974_B1Dkd.seq check
            >D07990B5_B1Dk_1_EF1a-N_R_B08_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGNCGA NGTGCGCTCT GCCCNCTGAC GGGCACCGGA
               61 NCCAATTCCC ACTCCTTTCA AGACCTAGAA GGTCCATTAG CTGCAAAGAT TCCTCTCTGT
              121 TTAAAACTTT ATCCATCTTT GCAAAGCTTT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT
              181 CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA
              241 AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG
              301 GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT
              361 CTGCCTGCTG GGGAGGGAAT TAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG
              421 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC
              481 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT
              541 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG
              601 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT
              661 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CNACAGAATC
              721 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGNCAG CAAAAGGCCA GGAANCGTAA
              781 AAAGGNCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGANGAGC ATCACAAAAA
              841 TCGACGCTCA AGTCANANGT GGCGAAACCC GACAGGACTA TAAAGATACC ANGCGTTNCC
              901 CCCNGNAANC TCCCTCGTGC GCTCTCCNGT TNCGANCCTG CCGCTTANNN GATACCTGTC
              961 CGCCTTTCNN CNNNCNGGAA GCGTGGCGNT TTCNCATANC TCACGCTGNN NNNATCTCAN
            1021 NNCNGNNGNN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Suda, T., Purification and characterization of the Fas-ligand that induces apoptosis. J. Exp. Med. 179 (3): 873-879 (1994). PMID 7509364.

            2022.11.17

            GNP_filter3_RDBDEP_html_221010.pl