Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pEF321-Flag-Ex

Mammalian expression vector based on human EF-1 alpha promoter.

Catalog number RDB18973
Resource name pEF321-Flag-Ex
Clone info. Mammalian expression vector based on human EF-1 alpha promoter. An additional 5' flanking region (about 1.3 kb) of EF-1a was added to pEF-Flag-EX (RDB18972) (a total 2.5 kb fragment that includes the promoter, 1st exon, 1st intron and a part of exon 2 of human EF-1a gene). Please see Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994.
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin
Depositor|Developer Nagata, Shigekazu |
 
Sequence (full) RDB18973hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB18973_B1Lmp1-3.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

提供依頼書
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18973 pEF321-Flag-Ex DNA solution


How to cite this biological resource

Materials & Methods section:

The pEF321-Flag-Ex was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18973).

Reference section:

Wakabayashi-Ito, N., Nagata, S., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Wakabayashi-Ito, N., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976. [link to RRC of NBRP]