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pEF321-Flag-Ex (#RDB18973)

Mammalian expression vector based on human EF-1 alpha promoter.


Drawn by SnapGene® software
Sequence (full) RDB18973hts01.seq
Publication Wakabayashi-Ito, N., J. Biol. Chem. 269 (47): 29831-29837 (1994). [link to RRC of NBRP]
Test sheet RDB18973_B1Lmp1-3.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Mammalian expression vector based on human EF-1 alpha promoter. An additional 5' flanking region (about 1.3 kb) of EF-1a was added to pEF-Flag-EX (RDB18972) (a total 2.5 kb fragment that includes the promoter, 1st exon, 1st intron and a part of exon 2 of human EF-1a gene). Please see Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994.
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin
Depositor|Developer Nagata, Shigekazu |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

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RDB18973 pEF321-Flag-Ex DNA solution

How to cite this biological resource

Materials & Methods section:

The pEF321-Flag-Ex was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18973).

Reference section:

Wakabayashi-Ito, N., Nagata, S., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Wakabayashi-Ito, N., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976. [link to RRC of NBRP]