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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF321-Flag-Ex

Mammalian expression vector based on human EF-1 alpha promoter.

Catalog number RDB18973
Resource name pEF321-Flag-Ex
Clone info. Mammalian expression vector based on human EF-1 alpha promoter. An additional 5' flanking region (about 1.3 kb) of EF-1a was added to pEF-Flag-EX (RDB18972) (a total 2.5 kb fragment that includes the promoter, 1st exon, 1st intron and a part of exon 2 of human EF-1a gene). Please see Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994.
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin
Depositor|Developer Nagata, Shigekazu |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence (full) RDB18973zzt02.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18973 pEF321-Flag-Ex DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Wakabayashi-Ito, N., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976.

            Further references such as user reports and related articles (go to bottom)

            Featured content

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            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18973_B1Lmp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: EF-1 alpha pro (long) 5'
            Sequence file: RDB18973_B1Lmj.seq check
            >D07990B3_B1Lm _1_lacIpro-B_F_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCGA NTNATTAATG CAGCTGGCAC GACAGGTTTC
               61 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTANN
              121 NNNCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
              181 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG
              241 TCACTTAGCG TTGGCCACAT AGTAGGTTCT CAAATACTTG TTAATAAATA AGTTTGTTCG
              301 AGAAGCTGGG CAATGATATT CTACAGCTGG AAGAAGAAAC ATAATGATCT AGTAATTAGC
              361 TCATTAAAAA TAAACGTTCT TCTTTCCTCN NAGGAGCATT TCCCAAGGCC TGCCTTGATA
              421 GCCATCCAAA AAGGCCAAGC TCATCCAATC TTGCCCTAGA TTTATGCTAA AATGCAGTTA
              481 NNNNNNATAG GATGANNNAN AACNANNNCA CTTATTTAAA TATAATAGGC ACTTATTTAA
              541 ATAGGANAAG CTGNNNNTTC NTAGCAAGTG NTGGGGNNAN GAAANTGGGN NNGANAAACN
              601 TNGCTGATGC TGTANATCTT ANCCTCTNCN TGANANCATG NGNNAAATCT NNAAGCATTT
            661 NNNNNNCNTA NNNTTNNNNT CNANTNACNG NNNNNNNTTT NNNTNNNANN NNNNNNNN
            //
            Primer: EF1a-C_F
            Region: EF-1 alpha pro (long) 3', FLAG, hGCSF 3' UTR
            Sequence file: RDB18973_B1Lmk.seq check
            >D07990B3_B1Lm _1_EF1a-C_F_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTTGGTT CNTTCTCANG CCTCANACAG
               61 TGGTTCAAAG TTTTTTNNTT CCNTTTCAGG TGTCGTGAGG AATTACCATG GACTACAAAG
              121 ACGATGACGA TAAAGCTGGA GAATTCGGAT CCGCTAGCGT CGACTCTAGA GTGAGGGTCC
              181 CCACCTGGGA CCCTTGAGAG TATCAGGTCT CCCACGTGGG AGACAAGAAA TCCCTGTTTA
              241 ATATTTAAAC AGCAGTGTTC CCCATCTGGG TCCTTGCACC CCTCACTCTG GCCTCAGCCG
              301 ACTGCACAGC GGCCCCTGCA TCCCCTTGGC TGTGAGGCCC CTGGACAAGC AGAGGTGGCC
              361 AGAGCTGGGA GGCATGGCCC TGGGGTCCCA CGAATTTGCT GGGGAATCTC GTTTTTCTTC
              421 TTAAGACTTT TGGGACATGG TTTGACTCCC GAACATCACC GACGTGTCTC CTGTTTTTCT
              481 GGGTGGCCTC GGGACACCTG CCCTGCCCCC ACGAGGGTCA GGACTGTGAC TCTTTTTAGG
              541 GCCAGGCAGG TGCCTGGACA TTTGCCTTGC TGGACGGGGA CTGGGGANGT GGGANGGANC
              601 AGACAGGANG AATCATGTCA NGCCTGTGTG TGAAAGGAAG CTCCACTGTC ACCCTCNNCC
              661 TCTTCNCCCC CCACTCNCCA NTGNCCCCTC NNTGTNNCNT TGTANCTGNN NNNCNNNNNA
            721 NANNTGNTNG CNNCNNNNNN NNNN
            //
            Primer: EF1a-N_F3
            Region: EF-1 alpha pro (long) mid
            Sequence file: RDB18973_B1Lml.seq check
            >D07990B3_B1Lm_1_EF1a-N_F3_G03_21_ABI24.ab1
                1 NNNNNNNNNN NNNNGNNTGA NTCTATTCTA AACTGAAAGC CTGGACATCT GGAGTACCAG
               61 GGGGAGATGA CGTGTTACGG GCTTCCATAA AAGCAGCTGG CTTTGAATGG AAGGAGCCAA
              121 GAGGCCAGCA CAGGAGCGGA TTCGTCGCTT TCACGGCCAT CGAGCCGAAC CTCTCGCAAG
              181 TCCGTGAGCC GTTAAGGAGG CCCCCAGTCC CGACCCTTCG CCCCAAGCCC CTCGGGGTCC
              241 CCGGGCCTGG TACTCCTTGC CACACGGGAG GGGCGCGGAA GCCGGGGCGG AGGAGGAGCC
              301 AACCCCGGGC TGGGCTGAGA CCCGCAGAGG AAGACGCTCT AGGGATTTGT CCCGGACTAG
              361 CGAGATGGCA AGGCTGAGGA CGGGAGGCTG ATTGAGAGGC GAAGGTACAC CCTAATCTCA
              421 ATACAACCTT TGGAGCTAAG CCAGCAATGG TAGAGGGAAG ATTCTGCACG TCCCTTCCAG
              481 GCGGCCTCCC CGTCACCACC CCCCCCAACC CGCCCCGACC GGAGCTGAGA GTAATTCATA
              541 CAAAAGGACT CGCCCCTGCC TTGGGGAATC CCAGGGACCG TCGTTAAACT CCCACTAACG
              601 TAGAACCCAG AGATCGCTGC GTTCCCGCCC CCTCACCCGC CCGCTCTCGT CATCACTGAG
              661 GTGGAGAAGA GCATGCGTGA GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA
              721 CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAANGNGGC
              781 GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GANGGTGGGG
              841 GANAANCGTA TATAAGTGCA GTAGTCGCCG NGANCGTTCT TTTTCGCAAC GGGTTTGCCG
              901 CCAGAACACA NGTNANTGCC GNGTGTGGTN NCCGCGGGCC TNNNCTCTTT NNGGGNNNNG
              961 GNCCNNGCGT GCCNTGNATT NCTTNCNNGC CCCNGGNNGC NGNANGTGAN NNNNGNTCCC
            1021 NANCNNNNGN NNNNNNNNN
            //
            Primer: EF1a-M_R2
            Region: EF-1 alpha pro (long) mid
            Sequence file: RDB18973_B1Lmm.seq check
            >D07990B3_B1Lm_1_EF1a-M_R2_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN GGGCGNNNGG NCGGGNNTGG GGGCCTCCTT ANCGGCTCNC
               61 GGACTTGCNN NNNNNNNNNN NNNNGGCCGT GAAAGCGACG AATCCGCTCN NNNNNNNNNN
              121 NCTTGGCTCC TTCCATTCAA AGCCAGCTGC TTTTATGGAA GCCCGTAACA CGTCATCTCC
              181 CCCTGGTACT CCAGATGTCC AGGCTTTCAG TTTAGAATAG ACTCAGTCCT ACAGTTAGCT
              241 TTAGATCTAA TTCTAGTTTT GTTACGCCAA AAAGTTCCTG CGAGTGTGTG TGTGTGCCTC
              301 ATGGTACTTT TTAAATTAAA AGGTGTACAG TTATTTGATT GCAAACATAA GGAACCTAAA
              361 ATGCTTTCAG ATTTTCCACA TGATCTCATG TAGAGGCTAA GATCTACAGC ATCAGCAAGT
              421 TTATCCACCC AGTTTCCTAA CCCCAACACT TGCTATGAAG TCACAGCTTC TCCTATTTAA
              481 ATAAGTGCCT ATTATATTTA AATAAGTGCT GTCGTTTTCT GTCATCCTAT CGATTGTAAC
              541 TGCATTTTAG CATAAATCTA GGGCAAGATT GGATGAGCTT GGCCTTTTTG GATGGCTATC
              601 AAGGCAGGCC TTGGGAAATG CTCCTCTGAG GAAAGAAGAA CGTTTATTTT TAATGAGCTA
              661 ATTACTAGAT CATTATGTTT CTTCTTCCAG CTGTAGAATA TCATTGNNNN NNTTCTCGAA
              721 CAAACTTATT TATTAACAAG TATTTGANAA CCTACTATGT GGCCAACGCT NAGTGANCTG
              781 CANGCATGCN AGCTTGGCGN AANNCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC
              841 CGCTNNCAAT TCCACACAAC ATACNAGCCN GGAAGCATAA AGTGTAAANG NCNNGGGGTG
              901 NNNNANNGAG TGAGCTANNN CNCATNAANT TGNGTTGCGC TCACNGNCCC NCTTNCNNNN
            961 NNNNAANNCT GNCNNGNCNN CTNNCNTTNA NNGAATNGNC NNNNNNNNNN
            //
            Primer: EF1a-M_F2
            Region: EF-1 alpha pro (long) mid
            Sequence file: RDB18973_B1Lmn.seq check
            >D07990B3_B1Lm_1_EF1a-M_F2_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NCNNNNCTGN NNTGGANAAG AGCNNGNNNG AGGCTCCGGT
               61 GCCCGTCAGT GNNNNNNNNN NNNNNNNCCN CAGTCCCCGA GAAGTTGGGG GGNNNNNNNN
              121 NNNNNTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT
              181 ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG
              241 TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT
              301 CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACGC
              361 CCCTGGCTGC AGTACGTGAT TCTTGATCCC GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT
              421 TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG
              481 CGCTGGGGCC GCCGCGTGCG AATCTGGTGG CACCTTCGCG CCTGTCTCGC TGCTTTCGAT
              541 AAGTCTCTAG CCATTTAAAA TTTTTGATGA CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT
              601 AGTCTTGTAA ATGCGGGCCA AGATCTGCAC ACTGGTATTT CGGTTTTTGG GGCCGCGGGC
              661 GGCGACGGGG CCCGTGCGTC CCANCNCACA TGTTCGGCGA GGCGGGGCCT GCNAGCGCGG
              721 CCNCCNANAA TCGGACGGGG GTAGTCTCAA GCTGGCCGGC CTGCTCTGGT GNCNTGNNCT
              781 CGCGCCNCCG TGTATCGCNC CGCNCTGNGC GGNNAGGCTG GCCCGGTCGG CACCNGTTGC
              841 GTGAGCNGNA AAGATGGNCN CNTCCCNGNC CTGCTGNNNG GAGCTNNNNN NGGANGACNN
              901 NGNGNTNGGG NANANCGGGC GGGTGANNCA CCCANNCNAA NNNNNNGNCC NTNNCCNNNN
            961 NNNNNN
            //
            Primer: hGCSF_R
            Region: EF-1 alpha pro (long) mid
            Sequence file: RDB18973_B1Lmo.seq check
            >D07990B3_B1Lm_1_hGCSF_R_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGTCCNNGGT GGGGNNCCTC ACTCTAGAGT CGACGCTAGC
               61 GGATCCGAAN TCTCCAGCTT TATCGTCATC GTCTTTGTAG TCCATGGTAA TTCCTCACGA
              121 CACCTGAAAT GGAAGAAAAA AACTTTGAAC CACTGTCTGA GGCTTGAGAA TGAACCAAGA
              181 TCCAAACTCA AAAAGGGCAA ATTCCAAGGA GAATTACATC AAGTGCCAAG CTGGCCTAAC
              241 TTCAGTCTCC ACCCACTCAG TGTGGGGAAA CTCCATCGCA TAAAACCCCT CCCCCCAACC
              301 TAAAGACGAC GTACTCCAAA AGCTCGAGAA CTAATCGAGG TGCCTGGACG GCGCCCGGTA
              361 CTCCGTGGAG TCACATGAAG CGACGGCTGA GGACGGAAAG GCCCTTTTCC TTTGTGTGGG
              421 TGACTCACCC GCCCGCTCTC CCGAGCGCCG CGTCCTCCAT TTTGAGCTCC CTGCAGCAGG
              481 GCCGGGAAGC GGCCATCTTT CCGCTCACGC AACTGGTGCC GACCGGGCCA GCCTTGCCGC
              541 CCAGGGCGGG GCGATACACG GCGGCGCGAG GCCAGGCACC AGAGCAGGCC GGCCAGCTTG
              601 AGACTACCCC CGTCCGATTC TCGGTGGCCG CGCTCGCAGG CCCCGCCTCG CCGAACATGT
              661 GCGCTGGGAC GCACGGGCCC CGTCGCCGCC CGCGGCCCCA AAAACCGAAA TACCAGTGTG
              721 CAGATCTTGG CCCGCATTTA CAAGACTATC TTGCCAGANA AAAAGCGTCG CAGCANGTCA
              781 TCAAAAATTT TAAATGGCTA GAGANTTATC GAAAGCAGCG AGACAGGCGC GAANGTGCCA
              841 CCAGATTCGC ACGCGGCGGN CCCAGCGCCC AGGNCAGGNC TCNACTCNAG CACGANGCGN
              901 ANNGGGCTCC NTAAGCNCAA GGNCTCNAAC TCTCCCNNCC NCTTCCNACC CGAANCTCGG
              961 GNNCNNGANN CACGTACTGC ANCCNGGGGN GNNNNANNTN NNTCANGGNA CNNNANGNNC
            1021 NNTNNNCNNN AANNNNNNNN GNNCNNNNGG NNNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Wakabayashi-Ito, N., Characterization of the regulatory elements in the promoter of the human elongation factor-1 alpha gene. J. Biol. Chem. 269 (47): 29831-29837 (1994). PMID 7961976.

            2022.09.29

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