Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF321-Flag-Ex

Mammalian expression vector based on human EF-1 alpha promoter.

Sequence (full)	RDB18973zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990), (Wakabayashi-Ito, N. and Nagata, S., J. Biol. Chem., 269 (47): 29831-29837, 1994)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18973_B1Lmp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: EF-1 alpha pro (long) 5' Sequence file: RDB18973_B1Lmj.seq
>D07990B3_B1Lm _1_lacIpro-B_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCGA NTNATTAATG CAGCTGGCAC GACAGGTTTC    61 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTANN   121 NNNCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT   181 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG   241 TCACTTAGCG TTGGCCACAT AGTAGGTTCT CAAATACTTG TTAATAAATA AGTTTGTTCG   301 AGAAGCTGGG CAATGATATT CTACAGCTGG AAGAAGAAAC ATAATGATCT AGTAATTAGC   361 TCATTAAAAA TAAACGTTCT TCTTTCCTCN NAGGAGCATT TCCCAAGGCC TGCCTTGATA   421 GCCATCCAAA AAGGCCAAGC TCATCCAATC TTGCCCTAGA TTTATGCTAA AATGCAGTTA   481 NNNNNNATAG GATGANNNAN AACNANNNCA CTTATTTAAA TATAATAGGC ACTTATTTAA   541 ATAGGANAAG CTGNNNNTTC NTAGCAAGTG NTGGGGNNAN GAAANTGGGN NNGANAAACN   601 TNGCTGATGC TGTANATCTT ANCCTCTNCN TGANANCATG NGNNAAATCT NNAAGCATTT 661 NNNNNNCNTA NNNTTNNNNT CNANTNACNG NNNNNNNTTT NNNTNNNANN NNNNNNNN //
Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro (long) 3', FLAG, hGCSF 3' UTR Sequence file: RDB18973_B1Lmk.seq
>D07990B3_B1Lm _1_EF1a-C_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTTGGTT CNTTCTCANG CCTCANACAG    61 TGGTTCAAAG TTTTTTNNTT CCNTTTCAGG TGTCGTGAGG AATTACCATG GACTACAAAG   121 ACGATGACGA TAAAGCTGGA GAATTCGGAT CCGCTAGCGT CGACTCTAGA GTGAGGGTCC   181 CCACCTGGGA CCCTTGAGAG TATCAGGTCT CCCACGTGGG AGACAAGAAA TCCCTGTTTA   241 ATATTTAAAC AGCAGTGTTC CCCATCTGGG TCCTTGCACC CCTCACTCTG GCCTCAGCCG   301 ACTGCACAGC GGCCCCTGCA TCCCCTTGGC TGTGAGGCCC CTGGACAAGC AGAGGTGGCC   361 AGAGCTGGGA GGCATGGCCC TGGGGTCCCA CGAATTTGCT GGGGAATCTC GTTTTTCTTC   421 TTAAGACTTT TGGGACATGG TTTGACTCCC GAACATCACC GACGTGTCTC CTGTTTTTCT   481 GGGTGGCCTC GGGACACCTG CCCTGCCCCC ACGAGGGTCA GGACTGTGAC TCTTTTTAGG   541 GCCAGGCAGG TGCCTGGACA TTTGCCTTGC TGGACGGGGA CTGGGGANGT GGGANGGANC   601 AGACAGGANG AATCATGTCA NGCCTGTGTG TGAAAGGAAG CTCCACTGTC ACCCTCNNCC   661 TCTTCNCCCC CCACTCNCCA NTGNCCCCTC NNTGTNNCNT TGTANCTGNN NNNCNNNNNA 721 NANNTGNTNG CNNCNNNNNN NNNN //
Primer: EF1a-N_F3 (Pr1248) Region: EF-1 alpha pro (long) mid Sequence file: RDB18973_B1Lml.seq
>D07990B3_B1Lm_1_EF1a-N_F3_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNGNNTGA NTCTATTCTA AACTGAAAGC CTGGACATCT GGAGTACCAG    61 GGGGAGATGA CGTGTTACGG GCTTCCATAA AAGCAGCTGG CTTTGAATGG AAGGAGCCAA   121 GAGGCCAGCA CAGGAGCGGA TTCGTCGCTT TCACGGCCAT CGAGCCGAAC CTCTCGCAAG   181 TCCGTGAGCC GTTAAGGAGG CCCCCAGTCC CGACCCTTCG CCCCAAGCCC CTCGGGGTCC   241 CCGGGCCTGG TACTCCTTGC CACACGGGAG GGGCGCGGAA GCCGGGGCGG AGGAGGAGCC   301 AACCCCGGGC TGGGCTGAGA CCCGCAGAGG AAGACGCTCT AGGGATTTGT CCCGGACTAG   361 CGAGATGGCA AGGCTGAGGA CGGGAGGCTG ATTGAGAGGC GAAGGTACAC CCTAATCTCA   421 ATACAACCTT TGGAGCTAAG CCAGCAATGG TAGAGGGAAG ATTCTGCACG TCCCTTCCAG   481 GCGGCCTCCC CGTCACCACC CCCCCCAACC CGCCCCGACC GGAGCTGAGA GTAATTCATA   541 CAAAAGGACT CGCCCCTGCC TTGGGGAATC CCAGGGACCG TCGTTAAACT CCCACTAACG   601 TAGAACCCAG AGATCGCTGC GTTCCCGCCC CCTCACCCGC CCGCTCTCGT CATCACTGAG   661 GTGGAGAAGA GCATGCGTGA GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA   721 CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAANGNGGC   781 GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GANGGTGGGG   841 GANAANCGTA TATAAGTGCA GTAGTCGCCG NGANCGTTCT TTTTCGCAAC GGGTTTGCCG   901 CCAGAACACA NGTNANTGCC GNGTGTGGTN NCCGCGGGCC TNNNCTCTTT NNGGGNNNNG   961 GNCCNNGCGT GCCNTGNATT NCTTNCNNGC CCCNGGNNGC NGNANGTGAN NNNNGNTCCC 1021 NANCNNNNGN NNNNNNNNN //
Primer: EF1a-M_R2 (Pr1244) Region: EF-1 alpha pro (long) mid Sequence file: RDB18973_B1Lmm.seq
>D07990B3_B1Lm_1_EF1a-M_R2_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GGGCGNNNGG NCGGGNNTGG GGGCCTCCTT ANCGGCTCNC    61 GGACTTGCNN NNNNNNNNNN NNNNGGCCGT GAAAGCGACG AATCCGCTCN NNNNNNNNNN   121 NCTTGGCTCC TTCCATTCAA AGCCAGCTGC TTTTATGGAA GCCCGTAACA CGTCATCTCC   181 CCCTGGTACT CCAGATGTCC AGGCTTTCAG TTTAGAATAG ACTCAGTCCT ACAGTTAGCT   241 TTAGATCTAA TTCTAGTTTT GTTACGCCAA AAAGTTCCTG CGAGTGTGTG TGTGTGCCTC   301 ATGGTACTTT TTAAATTAAA AGGTGTACAG TTATTTGATT GCAAACATAA GGAACCTAAA   361 ATGCTTTCAG ATTTTCCACA TGATCTCATG TAGAGGCTAA GATCTACAGC ATCAGCAAGT   421 TTATCCACCC AGTTTCCTAA CCCCAACACT TGCTATGAAG TCACAGCTTC TCCTATTTAA   481 ATAAGTGCCT ATTATATTTA AATAAGTGCT GTCGTTTTCT GTCATCCTAT CGATTGTAAC   541 TGCATTTTAG CATAAATCTA GGGCAAGATT GGATGAGCTT GGCCTTTTTG GATGGCTATC   601 AAGGCAGGCC TTGGGAAATG CTCCTCTGAG GAAAGAAGAA CGTTTATTTT TAATGAGCTA   661 ATTACTAGAT CATTATGTTT CTTCTTCCAG CTGTAGAATA TCATTGNNNN NNTTCTCGAA   721 CAAACTTATT TATTAACAAG TATTTGANAA CCTACTATGT GGCCAACGCT NAGTGANCTG   781 CANGCATGCN AGCTTGGCGN AANNCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC   841 CGCTNNCAAT TCCACACAAC ATACNAGCCN GGAAGCATAA AGTGTAAANG NCNNGGGGTG   901 NNNNANNGAG TGAGCTANNN CNCATNAANT TGNGTTGCGC TCACNGNCCC NCTTNCNNNN 961 NNNNAANNCT GNCNNGNCNN CTNNCNTTNA NNGAATNGNC NNNNNNNNNN //
Primer: EF1a-M_F2 (Pr1243) Region: EF-1 alpha pro (long) mid Sequence file: RDB18973_B1Lmn.seq
>D07990B3_B1Lm_1_EF1a-M_F2_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNNNNCTGN NNTGGANAAG AGCNNGNNNG AGGCTCCGGT    61 GCCCGTCAGT GNNNNNNNNN NNNNNNNCCN CAGTCCCCGA GAAGTTGGGG GGNNNNNNNN   121 NNNNNTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT   181 ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG   241 TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT   301 CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACGC   361 CCCTGGCTGC AGTACGTGAT TCTTGATCCC GAGCTTCGGG TTGGAAGTGG GTGGGAGAGT   421 TCGAGGCCTT GCGCTTAAGG AGCCCCTTCG CCTCGTGCTT GAGTTGAGGC CTGGCCTGGG   481 CGCTGGGGCC GCCGCGTGCG AATCTGGTGG CACCTTCGCG CCTGTCTCGC TGCTTTCGAT   541 AAGTCTCTAG CCATTTAAAA TTTTTGATGA CCTGCTGCGA CGCTTTTTTT CTGGCAAGAT   601 AGTCTTGTAA ATGCGGGCCA AGATCTGCAC ACTGGTATTT CGGTTTTTGG GGCCGCGGGC   661 GGCGACGGGG CCCGTGCGTC CCANCNCACA TGTTCGGCGA GGCGGGGCCT GCNAGCGCGG   721 CCNCCNANAA TCGGACGGGG GTAGTCTCAA GCTGGCCGGC CTGCTCTGGT GNCNTGNNCT   781 CGCGCCNCCG TGTATCGCNC CGCNCTGNGC GGNNAGGCTG GCCCGGTCGG CACCNGTTGC   841 GTGAGCNGNA AAGATGGNCN CNTCCCNGNC CTGCTGNNNG GAGCTNNNNN NGGANGACNN   901 NGNGNTNGGG NANANCGGGC GGGTGANNCA CCCANNCNAA NNNNNNGNCC NTNNCCNNNN 961 NNNNNN //
Primer: hGCSF_R (Pr0031) Region: EF-1 alpha pro (long) mid Sequence file: RDB18973_B1Lmo.seq
>D07990B3_B1Lm_1_hGCSF_R_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGTCCNNGGT GGGGNNCCTC ACTCTAGAGT CGACGCTAGC    61 GGATCCGAAN TCTCCAGCTT TATCGTCATC GTCTTTGTAG TCCATGGTAA TTCCTCACGA   121 CACCTGAAAT GGAAGAAAAA AACTTTGAAC CACTGTCTGA GGCTTGAGAA TGAACCAAGA   181 TCCAAACTCA AAAAGGGCAA ATTCCAAGGA GAATTACATC AAGTGCCAAG CTGGCCTAAC   241 TTCAGTCTCC ACCCACTCAG TGTGGGGAAA CTCCATCGCA TAAAACCCCT CCCCCCAACC   301 TAAAGACGAC GTACTCCAAA AGCTCGAGAA CTAATCGAGG TGCCTGGACG GCGCCCGGTA   361 CTCCGTGGAG TCACATGAAG CGACGGCTGA GGACGGAAAG GCCCTTTTCC TTTGTGTGGG   421 TGACTCACCC GCCCGCTCTC CCGAGCGCCG CGTCCTCCAT TTTGAGCTCC CTGCAGCAGG   481 GCCGGGAAGC GGCCATCTTT CCGCTCACGC AACTGGTGCC GACCGGGCCA GCCTTGCCGC   541 CCAGGGCGGG GCGATACACG GCGGCGCGAG GCCAGGCACC AGAGCAGGCC GGCCAGCTTG   601 AGACTACCCC CGTCCGATTC TCGGTGGCCG CGCTCGCAGG CCCCGCCTCG CCGAACATGT   661 GCGCTGGGAC GCACGGGCCC CGTCGCCGCC CGCGGCCCCA AAAACCGAAA TACCAGTGTG   721 CAGATCTTGG CCCGCATTTA CAAGACTATC TTGCCAGANA AAAAGCGTCG CAGCANGTCA   781 TCAAAAATTT TAAATGGCTA GAGANTTATC GAAAGCAGCG AGACAGGCGC GAANGTGCCA   841 CCAGATTCGC ACGCGGCGGN CCCAGCGCCC AGGNCAGGNC TCNACTCNAG CACGANGCGN   901 ANNGGGCTCC NTAAGCNCAA GGNCTCNAAC TCTCCCNNCC NCTTCCNACC CGAANCTCGG   961 GNNCNNGANN CACGTACTGC ANCCNGGGGN GNNNNANNTN NNTCANGGNA CNNNANGNNC 1021 NNTNNNCNNN AANNNNNNNN GNNCNNNNGG NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl