RDB18972 - pEF-Flag-EX

pEF-Flag-EX

Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein.

Catalog number	RDB18972
Resource name	pEF-Flag-EX
Clone info.	Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein. A Flag-tag with preceded ATG was inserted between EcoR1 and BamH1 of pEF-BOS-EX (RDB07940).
Vector backbone	pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers	Ampicillin
Depositor\|Developer	Nagata, Shigekazu \|

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External Database

Reference sequence

Sequence (full)	RDB18972zzk01.seq provided by the depositor
Remarks, protocol and/or map (png)	RDB18972_map.png

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Order form [Credit Card Payment] [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB18972	pEF-Flag-EX	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Original reference

Featured content	Mammalian Expression Vector, pEF-BOS series (English text)
Featured content	哺乳動物細胞用の遺伝子発現ベクター　pEF-BOSシリーズ (Japanese text)
Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl