RDB18972 - pEF-Flag-EX

pEF-Flag-EX (#RDB18972)

Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Unpublished bioresource
Test sheet	Data Sheet open

Clone info.	Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein. A Flag-tag with preceded ATG was inserted between EcoR1 and BamH1 of pEF-BOS-EX (RDB07940).
Vector backbone	pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers	Ampicillin
Growth conditions	LB+Ampicillin, 37oC
Depositor\|Developer	Nagata, Shigekazu \|

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog #	Resource name	Shipping form	Fee
RDB18972	pEF-Flag-EX	DNA solution

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Materials & Methods section:

The pEF-Flag-EX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18972).

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