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pEF-Flag-EX

Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein.

Catalog number RDB18972
Resource name pEF-Flag-EX
Clone info. Mammalian expression vector based on human EF-1 alpha promoter for an N-terminally Flag tagged recombinant protein. A Flag-tag with preceded ATG was inserted between EcoR1 and BamH1 of pEF-BOS-EX (RDB07940).
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin
Depositor|Developer Nagata, Shigekazu |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB18972 pEF-Flag-EX Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Sequence (full) RDB18972zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18972_map.png

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Mammalian Expression Vector, pEF-BOS series (English text)
            Featured content 哺乳動物細胞用の遺伝子発現ベクター pEF-BOSシリーズ (Japanese text)

            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles


            2022.05.24

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