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pPEF-BOS-EX

Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene.

Catalog number RDB18971
Resource name pPEF-BOS-EX
Clone info. Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene. The SV40 promoter-driven puromycin-resistant gene was transferred into pEF-BOS-EX (RDB07940).
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Depositor|Developer Nagata, Shigekazu |
 
Sequence (full) RDB18971hts01.seqcheck assembled from experimentally sequenced data
Remarks, protocol and/or map (png) RDB18971_map.png
Test sheet RDB18971_B1Dkp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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MTA, for use for not-for-profit academic purpose [Word]
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
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提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
提供同意書 (MTA, 営利目的用)[Word]
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RDB18971 pPEF-BOS-EX DNA solution


How to cite this biological resource

Materials & Methods section:

The pPEF-BOS-EX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18971).

Reference section:

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles