RDB18971 - pPEF-BOS-EX

Resource data sheet
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pPEF-BOS-EX

Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene.

Catalog number	RDB18971
Resource name	pPEF-BOS-EX
Clone info.	Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene. The SV40 promoter-driven puromycin-resistant gene was transferred into pEF-BOS-EX (RDB07940).
Vector backbone	pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers	Ampicillin (E. coli), puromycin (mammalian cell)
Depositor\|Developer	Nagata, Shigekazu \|

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External Database

Reference sequence

Sequence (full)	RDB18971hts01.seq assembled from experimentally sequenced data
Remarks, protocol and/or map (png)	RDB18971_map.png
Test sheet	RDB18971_B1Dkp1-1.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18971	pPEF-BOS-EX	DNA solution

How to cite this biological resource

Materials & Methods section:

The pPEF-BOS-EX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18971).

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Mammalian Expression Vector, pEF-BOS series (English text)
Featured content	哺乳動物細胞用の遺伝子発現ベクター　pEF-BOSシリーズ (Japanese text)
Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

References

Original, user report and related articles

2024.02.29

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