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pPEF-BOS-EX

Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene.

Catalog number RDB18971
Resource name pPEF-BOS-EX
Clone info. Mammalian expression vector based on human EF-1 alpha promoter with puromycin-resistant gene. The SV40 promoter-driven puromycin-resistant gene was transferred into pEF-BOS-EX (RDB07940).
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Depositor|Developer Nagata, Shigekazu |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB18971zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18971_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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            MTA, for use for not-for-profit academic purpose [Word]
            MTA, for use for for-profit purpose [Word]
            Please visit Information of Request for Distribution.[link] 
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
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            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            提供同意書 (MTA, 営利目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB18971 pPEF-BOS-EX Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pPEF-BOS-EX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18971).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Mammalian Expression Vector, pEF-BOS series (English text)
            Featured content 哺乳動物細胞用の遺伝子発現ベクター pEF-BOSシリーズ (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles


            2023.10.05

            GNP_filter3_RDBDEP_html_231001.pl