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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pNEF-BOS-EX (#RDB18970)

Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene.

Clone info. Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene. The SV40 promoter-driven G-418 resistant gene was transferred into pEF-BOS-EX (RDB07940).
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Depositor|Developer Nagata, Shigekazu |
 
Sequence (full) RDB18970zzk01.seq provided by the depositor
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Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

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RDB18970 pNEF-BOS-EX DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pNEF-BOS-EX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18970).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18970_B1Dkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: SV40 pro_ori.1, EF-1alpha pro
Sequence file: RDB18970_B1Dka.seq check
>D07990B1_B1Dk_2_lacIpro-B_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNNNNNNCNG TGNNCGANGA ANCGGAANAN CGCCCAATAC
   61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC
  121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
  181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
  241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTAAT TCCCTCCCCA
  301 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
  361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
  421 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
  481 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTGCAA AGATGGATAA
  541 AGTTTTAAAC AGAGAGGAAT CTTTGCAGCT AATGGACCTT CTAGGTCTTG AAAGGAGTGG
  601 GAATTGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
  661 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
  721 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGANAA CCGTATATAA
  781 GTGCAGTANT CGCCGNGAAC GTTCTTTTTT CGCAACGGGT TTGCCGCCNG AACACANGNA
  841 AGTGCCNNGN GNGGTNNCCG CGGGCCTGNN CNCTTNACNG NNTATGGNCC TTNCNNGNCN
901 NNNNNNNNTN NCACNNNNNN NNAGNACNNN ATTNNNNNTN NNNNNNNNN
//
Primer: EF1a-C_F (Pr0168)
Region: EF-1alpha pro,hG-CSF pA
Sequence file: RDB18970_B1Dkb.seq check
>D07990B1_B1Dk_2_EF1a-C_F_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT TTGNNTTTGG NNCTTGGTTC NTTCTCNAGC CTCNGACAGT
   61 GGTTCAAAGT TTTTTTCTTC CNTTTCAGGT GTCGTGAGGA ATTCGGTACC GCGGCCGCCC
  121 GGGGNTCCGC TAGCGTCGAC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC
  181 AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA
  241 TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC CCTGCATCCC
  301 CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA TGGCCCTGGG
  361 GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG ACATGGTTTG
  421 ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA CACCTGCCCT
  481 GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC TGGACATTTG
  541 CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC ATGTCAGGCC
  601 TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC TCACCAGTGT
  661 CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC CTCNNNNNAA
721 AAAANANNNN NNAAAAAAAN ANNANNNNNN ANNNNNNNNN N
//
Primer: Neo-M_R (Pr0145)
Region: NeoR,SV40 pro_ori.2
Sequence file: RDB18970_B1Dkc.seq check
>D07990B1_B1Dk_2_Neo-M_R_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTNCNNNNN NCTTNNGTGN NNACGTCNNN CACAGCTGCG
   61 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC
  121 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG
  181 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC
  241 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT
  301 CCTCATCCTG TCTCTTGATC AGATCCGAAA ATGGATATAC AAGCTCCCGG GAGCTTTTTG
  361 CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG
  421 GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG
  481 GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC
  541 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC
  601 CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG
  661 GACTTTCCAC ACCCTAACTG ACACACATTC CACAGGGATC TGATATCCAT ATGCCTGCAN
  721 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGNGGNNGT GCTGCAAGGC
  781 GATTAAGTTG GGTAACGCCA GGGTTTNCCC ANTCACGANG TTGTAAACGA NNNNNANNGN
841 AANTNNNGNN NNNNNNNN
//
Primer: AmpR_F (Pr0962)
Region: AmpR,ori
Sequence file: RDB18970_B1Dkd.seq check
>D07990B1_B1Dk_2_AmpR_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCGC TGNNNNAGGT GCCTCACTGA TTAAGCATTG
   61 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA
  121 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG
  181 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
  241 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
  301 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
  361 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
  421 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
  481 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
  541 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
  601 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
  661 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
  721 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
  781 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
  841 CTTTTTACGG NTCCTGNNCT TTTGNTGGCC TTTTGNTCAC ATGNTCTTNC CNGCGTTATC
  901 CCCTGATTCT GTGGATAACC GTATTANCNN CTTTGAGTGA NCTGANNCCG CTCGCCGCAN
  961 CCGAACGANC GANCGCANCG AGNNANTGAN CNANGNANNC NNANNANCNN CCNNNACNCA
1021 ANNCNNNNCN NNCCCNNNNN NNNNN
//
Primer: pEFbosNeo-M_F1 (Pr0913)
Region: hG-CSF pA
Sequence file: RDB18970_B1Dke.seq check
>D07990B1_B1Dk_2_pEFbosNeo-M_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NTGNNNNNNN NNCCCTGCAT CCCCTTGGCT
   61 GTGAGGCCCC TGGNCAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT GGGGTCCCAC
  121 GAATTTGCTG GGGAATCTCG TTTTTCTTCT TAAGACTTTT GGGACATGGT TTGACTCCCG
  181 AACATCACCG ACGTGTCTCC TGTTTTTCTG GGTGGCCTCG GGACACCTGC CCTGCCCCCA
  241 CGAGGGTCAG GACTGTGACT CTTTTTAGGG CCAGGCAGGT GCCTGGACAT TTGCCTTGCT
  301 GGACGGGGAC TGGGGATGTG GGAGGGAGCA GACAGGAGGA ATCATGTCAG GCCTGTGTGT
  361 GAAAGGAAGC TCCACTGTCA CCCTCCACCT CTTCACCCCC CACTCACCAG TGTCCCCTCC
  421 ACTGTCACAT TGTAACTGAA CTTCAGGATA ATAAAGTGTT TGCCTCCNAA AAAAAAAAAA
  481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
541 AAAAAAAAAN NNNNNNNTNN NNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro, SV40 pA,NeoR
Sequence file: RDB18970_B1Dkf.seq check
>D07990B1_B1Dk_2_Amp_R_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT GANCATTTAT CNGGGTTATT GTCTCNTGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGACG TCGACGGTAT ACAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA
  181 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
  241 TTGTAACCAT TATAAGCTGC AATAAACAAG TTGGGGTGGG CGAAGAACTC CAGCATGAGA
  301 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT
  361 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG
  421 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA
  481 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC
  541 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA
  601 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG
  661 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA
  721 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT
  781 CGACAAGANC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTNGGTGGT
  841 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCN NNCATGATGG
  901 ATANTTTCTC GNCNNGANCN AGGNGAGATG ANAGGANATN CTGCCCNNGN NCNTCNCCCN
  961 ANANNANCCA GTCCCTTCCN NCTTCNGTGA NNNCGTCNAN CACAGCTGCN NNANNNNNNG
1021 CCCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles