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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pNEF-BOS-EX

Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene.

Catalog number RDB18970
Resource name pNEF-BOS-EX
Clone info. Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene. The SV40 promoter-driven G-418 resistant gene was transferred into pEF-BOS-EX (RDB07940).
Vector backbone pEF-BOS-EX (RDB07940) (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Depositor|Developer Nagata, Shigekazu |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB18970zzk01.seq provided by the depositor
            Remarks, protocol and/or map (png) RDB18970_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB18970 pNEF-BOS-EX DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Mammalian Expression Vector, pEF-BOS series (English text)
            Featured content 哺乳動物細胞用の遺伝子発現ベクター pEF-BOSシリーズ (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB18970_B1Dkp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: lacIpro-B_F
            Region: SV40 pro_ori.1, EF-1alpha pro
            Sequence file: RDB18970_B1Dka.seq check
            >D07990B1_B1Dk_2_lacIpro-B_F_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGN NNNNNNNCNG TGNNCGANGA ANCGGAANAN CGCCCAATAC
               61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC
              121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
              181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
              241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTAAT TCCCTCCCCA
              301 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
              361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
              421 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
              481 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTGCAA AGATGGATAA
              541 AGTTTTAAAC AGAGAGGAAT CTTTGCAGCT AATGGACCTT CTAGGTCTTG AAAGGAGTGG
              601 GAATTGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT
              661 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG
              721 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGANAA CCGTATATAA
              781 GTGCAGTANT CGCCGNGAAC GTTCTTTTTT CGCAACGGGT TTGCCGCCNG AACACANGNA
              841 AGTGCCNNGN GNGGTNNCCG CGGGCCTGNN CNCTTNACNG NNTATGGNCC TTNCNNGNCN
            901 NNNNNNNNTN NCACNNNNNN NNAGNACNNN ATTNNNNNTN NNNNNNNNN
            //
            Primer: EF1a-C_F
            Region: EF-1alpha pro,hG-CSF pA
            Sequence file: RDB18970_B1Dkb.seq check
            >D07990B1_B1Dk_2_EF1a-C_F_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNTT TTGNNTTTGG NNCTTGGTTC NTTCTCNAGC CTCNGACAGT
               61 GGTTCAAAGT TTTTTTCTTC CNTTTCAGGT GTCGTGAGGA ATTCGGTACC GCGGCCGCCC
              121 GGGGNTCCGC TAGCGTCGAC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC
              181 AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA
              241 TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC CCTGCATCCC
              301 CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA TGGCCCTGGG
              361 GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG ACATGGTTTG
              421 ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA CACCTGCCCT
              481 GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC TGGACATTTG
              541 CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC ATGTCAGGCC
              601 TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC TCACCAGTGT
              661 CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC CTCNNNNNAA
            721 AAAANANNNN NNAAAAAAAN ANNANNNNNN ANNNNNNNNN N
            //
            Primer: Neo-M_R
            Region: NeoR,SV40 pro_ori.2
            Sequence file: RDB18970_B1Dkc.seq check
            >D07990B1_B1Dk_2_Neo-M_R_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNTNCNNNNN NCTTNNGTGN NNACGTCNNN CACAGCTGCG
               61 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC
              121 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG
              181 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC
              241 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT
              301 CCTCATCCTG TCTCTTGATC AGATCCGAAA ATGGATATAC AAGCTCCCGG GAGCTTTTTG
              361 CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG
              421 GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG
              481 GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC
              541 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC
              601 CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG
              661 GACTTTCCAC ACCCTAACTG ACACACATTC CACAGGGATC TGATATCCAT ATGCCTGCAN
              721 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGNGGNNGT GCTGCAAGGC
              781 GATTAAGTTG GGTAACGCCA GGGTTTNCCC ANTCACGANG TTGTAAACGA NNNNNANNGN
            841 AANTNNNGNN NNNNNNNN
            //
            Primer: AmpR_F
            Region: AmpR,ori
            Sequence file: RDB18970_B1Dkd.seq check
            >D07990B1_B1Dk_2_AmpR_F_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCGC TGNNNNAGGT GCCTCACTGA TTAAGCATTG
               61 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA
              121 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG
              181 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
              241 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
              301 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
              361 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
              421 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
              481 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
              541 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
              601 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
              661 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
              721 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
              781 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
              841 CTTTTTACGG NTCCTGNNCT TTTGNTGGCC TTTTGNTCAC ATGNTCTTNC CNGCGTTATC
              901 CCCTGATTCT GTGGATAACC GTATTANCNN CTTTGAGTGA NCTGANNCCG CTCGCCGCAN
              961 CCGAACGANC GANCGCANCG AGNNANTGAN CNANGNANNC NNANNANCNN CCNNNACNCA
            1021 ANNCNNNNCN NNCCCNNNNN NNNNN
            //
            Primer: pEFbosNeo-M_F1
            Region: hG-CSF pA
            Sequence file: RDB18970_B1Dke.seq check
            >D07990B1_B1Dk_2_pEFbosNeo-M_F_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NTGNNNNNNN NNCCCTGCAT CCCCTTGGCT
               61 GTGAGGCCCC TGGNCAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT GGGGTCCCAC
              121 GAATTTGCTG GGGAATCTCG TTTTTCTTCT TAAGACTTTT GGGACATGGT TTGACTCCCG
              181 AACATCACCG ACGTGTCTCC TGTTTTTCTG GGTGGCCTCG GGACACCTGC CCTGCCCCCA
              241 CGAGGGTCAG GACTGTGACT CTTTTTAGGG CCAGGCAGGT GCCTGGACAT TTGCCTTGCT
              301 GGACGGGGAC TGGGGATGTG GGAGGGAGCA GACAGGAGGA ATCATGTCAG GCCTGTGTGT
              361 GAAAGGAAGC TCCACTGTCA CCCTCCACCT CTTCACCCCC CACTCACCAG TGTCCCCTCC
              421 ACTGTCACAT TGTAACTGAA CTTCAGGATA ATAAAGTGTT TGCCTCCNAA AAAAAAAAAA
              481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
            541 AAAAAAAAAN NNNNNNNTNN NNNNNNNN
            //
            Primer: Amp_R
            Region: AmpR pro, SV40 pA,NeoR
            Sequence file: RDB18970_B1Dkf.seq check
            >D07990B1_B1Dk_2_Amp_R_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNT GANCATTTAT CNGGGTTATT GTCTCNTGAG CGGATACATA
               61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
              121 CCACCTGACG TCGACGGTAT ACAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA
              181 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
              241 TTGTAACCAT TATAAGCTGC AATAAACAAG TTGGGGTGGG CGAAGAACTC CAGCATGAGA
              301 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT
              361 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG
              421 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA
              481 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC
              541 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA
              601 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG
              661 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA
              721 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT
              781 CGACAAGANC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTNGGTGGT
              841 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCN NNCATGATGG
              901 ATANTTTCTC GNCNNGANCN AGGNGAGATG ANAGGANATN CTGCCCNNGN NCNTCNCCCN
              961 ANANNANCCA GTCCCTTCCN NCTTCNGTGA NNNCGTCNAN CACAGCTGCN NNANNNNNNG
            1021 CCCNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl