Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene.
Clone info. | Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene. The SV40 promoter-driven G-418 resistant gene was transferred into pEF-BOS-EX (RDB07940). |
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Vector backbone | pEF-BOS-EX (RDB07940) (plasmid) |
Selectable markers | Ampicillin (E. coli), Neomycin (mammalian cell). |
Depositor|Developer | Nagata, Shigekazu | |
Sequence (full) ![]() |
RDB18970zzk01.seq provided by the depositor | Additional map | ![]() |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18970 | pNEF-BOS-EX | DNA solution |
Materials & Methods section:
The pNEF-BOS-EX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18970). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18970_B1Dkp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: lacIpro-B_F (Pr0724) Region: SV40 pro_ori.1, EF-1alpha pro Sequence file: RDB18970_B1Dka.seq ![]() |
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>D07990B1_B1Dk_2_lacIpro-B_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NNNNNNNCNG TGNNCGANGA ANCGGAANAN CGCCCAATAC 61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG 181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT 241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTAAT TCCCTCCCCA 301 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 421 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 481 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTGCAA AGATGGATAA 541 AGTTTTAAAC AGAGAGGAAT CTTTGCAGCT AATGGACCTT CTAGGTCTTG AAAGGAGTGG 601 GAATTGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT 661 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG 721 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGANAA CCGTATATAA 781 GTGCAGTANT CGCCGNGAAC GTTCTTTTTT CGCAACGGGT TTGCCGCCNG AACACANGNA 841 AGTGCCNNGN GNGGTNNCCG CGGGCCTGNN CNCTTNACNG NNTATGGNCC TTNCNNGNCN 901 NNNNNNNNTN NCACNNNNNN NNAGNACNNN ATTNNNNNTN NNNNNNNNN // |
Primer: EF1a-C_F (Pr0168) Region: EF-1alpha pro,hG-CSF pA Sequence file: RDB18970_B1Dkb.seq ![]() |
>D07990B1_B1Dk_2_EF1a-C_F_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTT TTGNNTTTGG NNCTTGGTTC NTTCTCNAGC CTCNGACAGT 61 GGTTCAAAGT TTTTTTCTTC CNTTTCAGGT GTCGTGAGGA ATTCGGTACC GCGGCCGCCC 121 GGGGNTCCGC TAGCGTCGAC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC 181 AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA 241 TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC CCTGCATCCC 301 CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA TGGCCCTGGG 361 GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG ACATGGTTTG 421 ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA CACCTGCCCT 481 GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC TGGACATTTG 541 CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC ATGTCAGGCC 601 TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC TCACCAGTGT 661 CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC CTCNNNNNAA 721 AAAANANNNN NNAAAAAAAN ANNANNNNNN ANNNNNNNNN N // |
Primer: Neo-M_R (Pr0145) Region: NeoR,SV40 pro_ori.2 Sequence file: RDB18970_B1Dkc.seq ![]() |
>D07990B1_B1Dk_2_Neo-M_R_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTNCNNNNN NCTTNNGTGN NNACGTCNNN CACAGCTGCG 61 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC 121 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 181 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 241 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 301 CCTCATCCTG TCTCTTGATC AGATCCGAAA ATGGATATAC AAGCTCCCGG GAGCTTTTTG 361 CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG 421 GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG 481 GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC 541 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC 601 CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG 661 GACTTTCCAC ACCCTAACTG ACACACATTC CACAGGGATC TGATATCCAT ATGCCTGCAN 721 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGNGGNNGT GCTGCAAGGC 781 GATTAAGTTG GGTAACGCCA GGGTTTNCCC ANTCACGANG TTGTAAACGA NNNNNANNGN 841 AANTNNNGNN NNNNNNNN // |
Primer: AmpR_F (Pr0962) Region: AmpR,ori Sequence file: RDB18970_B1Dkd.seq ![]() |
>D07990B1_B1Dk_2_AmpR_F_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCGC TGNNNNAGGT GCCTCACTGA TTAAGCATTG 61 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 121 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 181 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 241 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 301 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 361 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 421 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 481 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 541 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 601 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 661 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 721 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 781 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 841 CTTTTTACGG NTCCTGNNCT TTTGNTGGCC TTTTGNTCAC ATGNTCTTNC CNGCGTTATC 901 CCCTGATTCT GTGGATAACC GTATTANCNN CTTTGAGTGA NCTGANNCCG CTCGCCGCAN 961 CCGAACGANC GANCGCANCG AGNNANTGAN CNANGNANNC NNANNANCNN CCNNNACNCA 1021 ANNCNNNNCN NNCCCNNNNN NNNNN // |
Primer: pEFbosNeo-M_F1 (Pr0913) Region: hG-CSF pA Sequence file: RDB18970_B1Dke.seq ![]() |
>D07990B1_B1Dk_2_pEFbosNeo-M_F_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NTGNNNNNNN NNCCCTGCAT CCCCTTGGCT 61 GTGAGGCCCC TGGNCAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT GGGGTCCCAC 121 GAATTTGCTG GGGAATCTCG TTTTTCTTCT TAAGACTTTT GGGACATGGT TTGACTCCCG 181 AACATCACCG ACGTGTCTCC TGTTTTTCTG GGTGGCCTCG GGACACCTGC CCTGCCCCCA 241 CGAGGGTCAG GACTGTGACT CTTTTTAGGG CCAGGCAGGT GCCTGGACAT TTGCCTTGCT 301 GGACGGGGAC TGGGGATGTG GGAGGGAGCA GACAGGAGGA ATCATGTCAG GCCTGTGTGT 361 GAAAGGAAGC TCCACTGTCA CCCTCCACCT CTTCACCCCC CACTCACCAG TGTCCCCTCC 421 ACTGTCACAT TGTAACTGAA CTTCAGGATA ATAAAGTGTT TGCCTCCNAA AAAAAAAAAA 481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 541 AAAAAAAAAN NNNNNNNTNN NNNNNNNN // |
Primer: Amp_R (Pr0008) Region: AmpR pro, SV40 pA,NeoR Sequence file: RDB18970_B1Dkf.seq ![]() |
>D07990B1_B1Dk_2_Amp_R_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNT GANCATTTAT CNGGGTTATT GTCTCNTGAG CGGATACATA 61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 121 CCACCTGACG TCGACGGTAT ACAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA 181 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT 241 TTGTAACCAT TATAAGCTGC AATAAACAAG TTGGGGTGGG CGAAGAACTC CAGCATGAGA 301 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT 361 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG 421 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA 481 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC 541 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA 601 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG 661 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA 721 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT 781 CGACAAGANC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTNGGTGGT 841 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCN NNCATGATGG 901 ATANTTTCTC GNCNNGANCN AGGNGAGATG ANAGGANATN CTGCCCNNGN NCNTCNCCCN 961 ANANNANCCA GTCCCTTCCN NCTTCNGTGA NNNCGTCNAN CACAGCTGCN NNANNNNNNG 1021 CCCNNNNNNN NNN // |
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Original, user report and related articles
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