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pHLUX20 (#RDB18951)

Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain.


Drawn by SnapGene® software
Sequence (full) RDB18951hts01.seq
Publication Iizumi, T., Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). [link to RRC of NBRP]
Publication Iizumi, T., Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). [link to RRC of NBRP]
Test sheet RDB18951_B3J5p1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain, see Fig. 1 of Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998.
Comment Expression was confirmed by the depositor with light emission in N. europaea.
Vector backbone pKT240 (plasmid)
Selectable markers Kanamycin
Growth remarks LB+Kanamycin, 37oC for E. coli; P medium + Kanamycin 30oC for N. europaea
Gene/insert name Vibrio harveyi luciferase genomic DNA; E. coli (Tn 903) kanamycin acetyltransferase genomic DNA; E. coli rho-independent terminator genomic DNA; Nitrosomonas europaea hydroxylamine oxidoreductase promoter genomic DNA
Depositor|Developer Iizumi, Taro |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

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RDB18951 pHLUX20 DNA solution

How to cite this biological resource

Materials & Methods section:

The pHLUX20 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18951).

Reference section:

Iizumi, T., Mizumoto, M., Nakamura, K., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781. [link to RRC of NBRP]
Iizumi, T., Nakamura, K., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Iizumi, T., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781. [link to RRC of NBRP]
original Iizumi, T., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112. [link to RRC of NBRP]