Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pHLUX20

Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain.

Catalog number RDB18951
Resource name pHLUX20
Clone info. Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain, see Fig. 1 of Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998.
Comment Expression was confirmed by the depositor with light emission in N. europaea.
Vector backbone pKT240 (plasmid)
Selectable markers Kanamycin
Growth remarks LB+Kanamycin, 37oC for E. coli; P medium + Kanamycin 30oC for N. europaea
Gene/insert name Vibrio harveyi luciferase genomic DNA; E. coli (Tn 903) kanamycin acetyltransferase genomic DNA; E. coli rho-independent terminator genomic DNA; Nitrosomonas europaea hydroxylamine oxidoreductase promoter genomic DNA
Depositor|Developer Iizumi, Taro |
 
Sequence (full) RDB18951hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB18951_B3J5p1-2.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Ordering
Order form [Credit Card Payment]  [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18951 pHLUX20 DNA solution


How to cite this biological resource

Materials & Methods section:

The pHLUX20 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18951).

Reference section:

Iizumi, T., Mizumoto, M., Nakamura, K., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781. [link to RRC of NBRP]
Iizumi, T., Nakamura, K., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Iizumi, T., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781. [link to RRC of NBRP]
original Iizumi, T., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112. [link to RRC of NBRP]