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pHLUX20

Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain.

Catalog number RDB18951
Resource name pHLUX20
Clone info. Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain, see Fig. 1 of Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998.
Comment Plasmid size: For 9,763 bp read "9,762 bp. Expression was confirmed by the depositor with light emission in N. europaea.
Vector backbone pKT240 (plasmid)
Selectable markers Kanamycin
Growth remarks LB+Kanamycin, 37oC for E. coli; P medium + Kanamycin 30oC for N. europaea
Gene/insert name Vibrio harveyi luciferase genomic DNA E. coli (Tn 903) kanamycin acetyltransferase genomic DNA E. coli rho-independent terminator genomic DNA Nitrosomonas europaea hydroxylamine oxidoreductase promoter genomic DNA
Depositor|Developer Iizumi, Taro |
Other clones in our bank

External Database
Vibrio harveyi luciferase
    E. coli (Tn 903) kanamycin acetyltransferase
      E. coli rho-independent terminator
        Nitrosomonas europaea hydroxylamine oxidoreductase promoter

            Reference sequence
              
             
            Sequence (full) RDB18951zzk01.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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            RDB18951 pHLUX20 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Iizumi, T., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781.
            original Iizumi, T., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Higher intensity luciferase (English text)
            Featured content Higher intensity luciferase (Japanese text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Iizumi, T., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781.
            original Iizumi, T., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112.

            2022.07.27

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