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pHLUX20

Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain.

Catalog number RDB18951
Resource name pHLUX20
Clone info. Expression vector of bacterial luciferase in Nitrosomonas europaea as a host strain, see Fig. 1 of Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998.
Plasmid size: For 9,763 bp read "9,762 bp. Expression was confirmed by the depositor with light emission in N. europaea.
Vector backbone pKT240 (plasmid)
Selectable markers Kanamycin
Growth remarks LB+Kanamycin, 37oC for E. coli; P medium + Kanamycin 30oC for N. europaea
Gene/insert name Vibrio harveyi luciferase genomic DNA E. coli (Tn 903) kanamycin acetyltransferase genomic DNA E. coli rho-independent terminator genomic DNA Nitrosomonas europaea hydroxylamine oxidoreductase promoter genomic DNA
Depositor|Developer Iizumi, Taro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Iizumi,T. et al., Appl. Environ. Microbiol., 64 (10): 3656-3662, 1998). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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RDB18951 pHLUX20 Under QC test. Please contact us. DNA solution Please contact us.

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Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Sequence (full) RDB18951zzk01.seq provided by the depositor

Original reference

original Iizumi, T., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781.
original Iizumi, T., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Iizumi, T., A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. Appl. Environ. Microbiol. 64 (10): 3656-3662 (1998). PMID 9758781.
original Iizumi, T., Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene. Appl. Environ. Microbiol. 63 (5): 1777-1784 (1997). PMID 9143112.

2021.08.31

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