Resource data sheet
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pEF-mTMEM16A (#RDB18756)

Expression vector of mouse Ano1 (Tmem16a).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Suzuki, J., J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839. [PubMed] [Article] [RRC of NBRP]
Publication Segawa, K., Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121. [PubMed] [Article] [RRC of NBRP]
Publication Suzuki, J., Nature 468 (7325): 834-838 (2010). PMID 21107324. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse anoctamin 1, calcium activated chloride channel (Ano1, Aliases: Tmem16a).
Vector backbone pEF-BOS-EX (plasmid)
Size of vector backbone 5.3 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name mouse Ano1 cDNA
Depositor|Developer Nagata, Shigekazu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引 用する (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010)。 利用者が教育・学術研究以外の目的で用いる場合、大阪大学と 別途 MTA を締結すること。
付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB18756 pEF-mTMEM16A DNA solution

How to cite this biological resource

Materials & Methods section:

The pEF-mTMEM16A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18756).

Reference section:

Suzuki, J., Fujii, T., Imao, T., Ishihara, K., Kuba, H., Nagata, S., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839. [PubMed] [Article] [RRC of NBRP]
Segawa, K., Suzuki, J., Nagata, S., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121. [PubMed] [Article] [RRC of NBRP]
Suzuki, J., Umeda, M., Sims, P.J., Nagata, S., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Suzuki, J., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839. [PubMed] [Article] [RRC of NBRP]
original Segawa, K., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121. [PubMed] [Article] [RRC of NBRP]
original Suzuki, J., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324. [PubMed] [Article] [RRC of NBRP]