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pMXs-puro-mTMEM16F-D409G

Retroviral expression vector of mouse anoctamin 6 Ano6 transcript variant 2 (Tmem16f) mutnat (D409G) tagged with FLAG at C-terminus.

Catalog number RDB18752
Resource name pMXs-puro-mTMEM16F-D409G
Clone info. Retroviral expression vector of mouse anoctamin 6 (Ano6 transcript variant 2, Aliases: Tmem16f) mutnat (D409G) tagged with FLAG at C-terminus. See Fig.1 of Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011.
Comment derivation：TMEM16F, Ba/F3 cell.
Vector backbone pMXs-puro (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name mouse Ano6 cDNA
Depositor|Developer Nagata, Shigekazu |
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mouse Ano6
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          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引 用する (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010)。 利用者が教育・学術研究以外の目的で用いる場合、大阪大学と 別途 MTA を締結すること。
          付加的提供条件：
          pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
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          提供同意書 (MTA, 非営利学術目的用)[Word]
          提供同意書 (MTA, 営利目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18752 pMXs-puro-mTMEM16F-D409G Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pMXs-puro-mTMEM16F-D409G was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18752).

          Reference section:

          Suzuki, J., Fujii, T., Imao, T., Ishihara, K., Kuba, H., Nagata, S., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839. [link to RRC of NBRP]
          Segawa, K., Suzuki, J., Nagata, S., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121. [link to RRC of NBRP]
          Suzuki, J., Umeda, M., Sims, P.J., Nagata, S., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Suzuki, J., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839. [link to RRC of NBRP]
          original Segawa, K., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121. [link to RRC of NBRP]
          original Suzuki, J., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324. [link to RRC of NBRP]

          2023.10.05

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