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pMXs-puro-mTMEM16J

Retroviral expression vector of mouse anoctamin 9 Ano9 (Tmem16j) tagged with FLAG at C-terminus.

Catalog number RDB18750
Resource name pMXs-puro-mTMEM16J
Clone info. Retroviral expression vector of mouse anoctamin 9 (Ano9, Aliases: Tmem16j) tagged with FLAG at C-terminus.
Comment derivation:TMEM16J, stomach.
Vector backbone pMXs-puro (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name mouse Ano9 cDNA
Depositor|Developer Nagata, Shigekazu |
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External Database
mouse Ano9

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
          Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引 用する (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010)。 利用者が教育・学術研究以外の目的で用いる場合、大阪大学と 別途 MTA を締結すること。
          付加的提供条件 pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18750 pMXs-puro-mTMEM16J Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Suzuki, J., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839.
          original Segawa, K., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121.
          original Suzuki, J., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Suzuki, J., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839.
          original Segawa, K., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121.
          original Suzuki, J., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324.

          2022.05.16

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