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pMXs-puro-mTMEM16A (#RDB18742)

Retroviral expression vector of mouse anoctamin 1 Ano1 (Tmem16a) tagged with FLAG at C-terminus.

Clone info. Retroviral expression vector of mouse anoctamin 1, calcium activated chloride channel (Ano1, Aliases: Tmem16a) tagged with FLAG at C-terminus.
Vector backbone pMXs-puro (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name mouse Ano1 cDNA
Depositor|Developer Nagata, Shigekazu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引 用する (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010)。 利用者が教育・学術研究以外の目的で用いる場合、大阪大学と 別途 MTA を締結すること。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18742 pMXs-puro-mTMEM16A Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pMXs-puro-mTMEM16A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18742).

Reference section:

Suzuki, J., Fujii, T., Imao, T., Ishihara, K., Kuba, H., Nagata, S., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839. [link to RRC of NBRP]
Segawa, K., Suzuki, J., Nagata, S., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121. [link to RRC of NBRP]
Suzuki, J., Umeda, M., Sims, P.J., Nagata, S., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Suzuki, J., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839. [link to RRC of NBRP]
original Segawa, K., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121. [link to RRC of NBRP]
original Suzuki, J., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324. [link to RRC of NBRP]