RDB18742 - pMXs-puro-mTMEM16A

Retroviral expression vector of mouse anoctamin 1 Ano1 (Tmem16a) tagged with FLAG at C-terminus.

Distribution information

Catalog number	RDB18742
Resource name	pMXs-puro-mTMEM16A
Clone info.	Retroviral expression vector of mouse anoctamin 1, calcium activated chloride channel (Ano1, Aliases: Tmem16a) tagged with FLAG at C-terminus.
Vector backbone	pMXs-puro (plasmid)
Selectable markers	Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name	mouse Ano1 cDNA
Depositor\|Developer	Nagata, Shigekazu \|

Other clones in our bank	mouse Ano1 (NCBI Gene 101772) \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions for distribution	Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

Distribution information (domestic users)

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Suzuki, J. et al., J. Biol. Chem., 288 (19): 13305-13316, 2013); (Segawa, K. et al., Proc. Natl. Acad. Sci. U S A., 108 (48): 19246-19251, 2011); (Suzuki, J. et al., Nature, 468 (7325): 834-838, 2010)。利用者が教育・学術研究以外の目的で用いる場合、大阪大学と別途 MTA を締結すること。
付加的提供条件	pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

References and tips

Sequence information

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB18742	pMXs-puro-mTMEM16A	Under QC test. Please contact us.	DNA solution

original	Suzuki, J., Calcium-dependent phospholipid scramblase activity of TMEM16 protein family members. J. Biol. Chem. 288 (19): 13305-13316 (2013). PMID 23532839.
original	Segawa, K., Constitutive exposure of phosphatidylserine on viable cells. Proc. Natl. Acad. Sci. U.S.A. 108 (48): 19246-19251 (2011). PMID 22084121.
original	Suzuki, J., Calcium-dependent phospholipid scrambling by TMEM16F. Nature 468 (7325): 834-838 (2010). PMID 21107324.

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.