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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

human ATP11C (#RDB18738)

Retroviral expression vector of human ATP11C.

Clone info. Retroviral expression vector of human ATPase phospholipid transporting 11C (ATP11C) tagged with FLAG at C-terminus.
Comment derivation：ATP11C, synthesized using a DNA synthesizer. Expression was confirmed by the depositor with microscopy.
Vector backbone pMXs-puro (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name human ATP11C cDNA
Depositor|Developer Nagata, Shigekazu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Segawa, K. et al., Science, 344 (6188): 1164-1168, 2014); (Segawa, K. et al., J. Biol. Chem., 291 (2): 762-772, 2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引 用する (Segawa, K. et al., Science, 344 (6188): 1164-1168, 2014); (Segawa, K. et al., J. Biol. Chem., 291 (2): 762-772, 2016)。 利用者が教育・学術研究以外の目的で用いる場合、大阪大学と 別途 MTA を締結すること。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18738 human ATP11C DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The human ATP11C was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18738).

Reference section:

Segawa, K., Kurata, S., Nagata, S., Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium. J. Biol. Chem. 291 (2): 762-772 (2016). PMID 26567335. [link to RRC of NBRP]
Segawa, K., Kurata, S., Yanagihashi, Y., Brummelkamp, T.R., Matsuda, F., Nagata, S., Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure. Science 344 (6188): 1164-1168 (2014). PMID 24904167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18738_B1C2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(hATP11C 5')
Sequence file: RDB18738_B1C2a.seq check
>D07512B5_B1C2_3_MMLV-pol_F_D05_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGN NNNNNCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC
   61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGCCACCAT
  121 GTTCAGGCGG AGCCTGAACA GATTCTGTGC CGGCGAAGAG AAGAGAGTGG GCACCAGAAC
  181 CGTGTTCGTG GGCAACCACC CCGTGTCCGA GACAGAGGCC TATATCGCCC AGAGATTCTG
  241 CGACAACAGA ATCGTGTCCA GCAAGTATAC CCTGTGGAAC TTCCTGCCCA AGAACCTGTT
  301 CGAGCAGTTC AGAAGAATCG CCAACTTCTA CTTCCTGATC ATCTTCCTGG TGCAAGTGAC
  361 CGTGGACACC CCCACCAGCC CTGTGACATC TGGCCTGCCC CTGTTCTTCG TGATCACCGT
  421 GACCGCCATC AAGCAGGGCT ACGAGGACTG GCTGAGACAC AGAGCCGACA ACGAAGTGAA
  481 CAAGAGCACC GTGTACATCA TCGAGAACGC CAAGAGAGTG CGGAAAGAGT CCGAGAAGAT
  541 CAAAGTGGGC GACGTGGTGG AAGTGCAGGC CGACGAGACA TTCCCCTGCG ACCTGATCCT
  601 GCTGAGCAGC TGCACCACCG ACGGCACCTG TTACGTGACC ACAGCCAGCC TGGACGGCGA
  661 GAGCAACTGC AAGACACACT ACGCCGTGCG GGACACAATC GCCCTGTGTA CCGCCGAGAG
  721 CATCGACACA CTGAGAGCCG CCATCGAGTG CGAGCAGCCT CAGCCCGACC TGTACAAGTT
  781 TGTGGGCAGA ATCAACATCT ACAGCAACAG CCTGNAANCC GTGNNCAGAA GCCTGGGCCC
  841 TGAGAACCTG CTGCTGAAGG GCGCCNCCCT GAAGAACACA GAGAANATCT ACNGCGTGNC
  901 CGNGNACNCC GGCNNNGNAA CNAAGATGGN CCTGAACTAC CANGGCNNGA NCCNGAAAAG
  961 NTNCNNCGNG NAAAANTCTA TCANCGCTTT CCTGATCGTG TNNNTGNNCN TTCNGNNGAN
1021 CNNNNNNGNC NNGTGNNNCN NNNNNNNNN
//
Primer: SV40pro_R2 (Pr0516)
Region: SV40 pro_ori,insert 3'(EGFP 3')
Sequence file: RDB18738_B1C2b.seq check
>D07512B5_B1C2_3_SV40pro_R2_E05_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT TCTNNCTGCT GGGGANCCTG GGGNNTTTCC NCACCCTAAC
   61 TGACACACAT TCCACAGGTC GACCACTGTG CTGGCGGCCG CTCGAGTTAC TTGTACAGCT
  121 CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC
  181 GCTTCTCGTT GGGGTCTTTG CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA
  241 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC
  301 TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT
  361 CGGCCATGAT ATAGACGTTG TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT
  421 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT
  481 CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT
  541 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG
  601 GGTAGCGGCT GAAGCACTGC ACGCCGTAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA
  661 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAN GTGGCATCGC
  721 CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC NGCTCGACCA
  781 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACGAATTCC AGCACGTTGG
  841 ANTCGTCGCT GAANGTCCNC ANCAGCAGTG GCCGCACAGA AGGNCTAGCA NANAGAGAGT
  901 CGCTGGNCCG NCNGCANCTC ANGNTTCTTC NGNNGCTTCT TCNGNGCACG TTCTTCANCA
  961 CGATCAGCAN AANCTCGGGG AANNNNNTGN NNAAGATCAN CANNANGNNN GNNNAGCNNN
1021 GTGGNNNCGC TNNNNNGCAT CTGNNCNNNN NCNNNNNNNA TNNNNNNNNN N
//
Primer: EGFP-N_R2 (Pr0905)
Region: insert mid(EGFP 5',(hATP11C 3')
Sequence file: RDB18738_B1C2c.seq check
>D07512B5_B1C2_3_EGFP-N_R2_F05_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNANNNN NGCNCGCCGT ANGTCNGGGT GGTCACGAGG
   61 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG
  121 TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG
  181 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACGAAT
  241 TCCAGCACGT TGGACTCGTC GCTGAAGGTC CGCAGCAGCA GTGGCCGCAC AGAAGGTCTA
  301 GCAGACAGAG AGTCGCTGGC CCGCCTGCAG CTCAGGTTTC TTCTGGCGCT TCTTCTGCGC
  361 ACGTTCTTCA GCACGATCAG CAGAATCTCG GGGAACAGGC TGATAAAGAT CAGCAGGATG
  421 ATGGCCAGCC ATGTGGACAC GCTGGACAGC ATCTGAGCGA ACACGAAGTA CATTCTCTGC
  481 TGCTTCAGAA AGGGCCAGAT GATCCCGCCC CAGAAGAAGG AGAAGAACAC GTAAAAGGCC
  541 AGGCTGCCCC AGATCACAAA GTGGTTGATC CAGGTCCAGA ATCTGGTGTC CAGGGCCAGC
  601 TTCAGGGTCA CTGTAAACAC CAGCACGGTA AACACGATGG TGCCGAAGGT CCAGTTGCCG
  661 TACACCTTGC CGTTCTCTTC CAGGGAGGCG GTTTGAAACA GGAAGTATGT TCCNAAAAAG
  721 AACACGGTGC CCTCGAAGGC GGCCAGGAAT GTCCAGTACA GGAAGGGGCC CAGCTGCAGC
  781 ATGGCGTTGC CGCTGATCTT CATGTACAGT CTGGGGTCNC TGGTCNNGGT GTCNANGTTG
  841 ATGTGCTGTT CCAGCNNNNT GTNNNCNGGA TGGGCANGNT GGTGAANCAN ANGTTGTACN
  901 TGGTCNNGNA NGCGGCGTCN TANAGGGNCT GNNGGNTGAN NNCNNNNAAN AANNGNNNCN
  961 NNANTNNNNN NNNNAAANCN CAGGTTTTTG NNNNAAAANT NNTGNNNNNN GTGANNNNTN
1021 NNNNCGNNNN ANNGGNGNNN GNGGGNNNNC ANNNNNTTTT NNNNNCTNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,PuroR
Sequence file: RDB18738_B1C2d.seq check
>D07512B5_B1C2_3_SV40pro_ori_F_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNGNNGCC TCGGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC
  121 TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT
  181 ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC
  241 AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG
  301 GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG
  361 AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG
  421 AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG
  481 TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG
  541 CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT
  601 TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA
  661 CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA
  721 NCNNNNGAGC GCACGACCCC ATGCATCGTC GACGATAAAA TAAAAGANTT TATTTAGTCT
  781 CCAGAAAAAN GGGGGAATGA AAGANCCCAC CTGTANGTTT GGNAAGCTAG CTTNANNNAC
  841 GCCNTTTTGN NNGGNATGGA AAAANACNTA ACTGANNNTA GAGAANTTCN NATCNNNGNT
  901 CANGNNCAGA TGGNNNAGCT GNNNANNGGN CNNAANNNGN NATCNGNNGN NNNCNNNTNN
961 NNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR
Sequence file: RDB18738_B1C2e.seq check
>D07512B5_B1C2_3_Amp_R_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNANCNNTT ATCNGGGTTA TTGTCTCNTG AGCGGATACA
   61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
  121 TGCCACCTGC ATAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC
  181 ATTTTGCAAG GCATGGAAAA ATACATAACT GAGAATAGAA AAGTTCAGAT CAAGGTCAGG
  241 AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC
  301 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT
  361 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC
  421 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC
  481 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC
  541 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG CGCGCCAGTC CTCCGATTGA
  601 CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG
  661 GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT
  721 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ANNNCTGCCC AGGGACCACC GACCCACCAC
  781 CGGGANGTAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG ATTGTCTAGT GTCTATGACT
  841 GNNTTTATGC GCCTGCGTCN GNACTANTNA GCNNACTAGC TCTGTATCTG GNGGANCCNN
  901 GGNGNNNNNG ANNANNTNNG GAANACCCNG NNNNNNNCCN NNGGNNANNN NNCNNGNACT
961 TNNGGNGNNN NNTTTNNNNN NNNNN
//
Primer: pMFG_1 (Pr0230)
Region: pol region,gag(truncated),MMLV psi
Sequence file: RDB18738_B1C2f.seq check
>D07512B5_B1C2_3_pMFG_1_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTTG TNNTGTNNGG GTCAGGGNAG TTTACAAGGG
   61 GCGGGGGTGC CCCATATAAG ATCTCATATG GCCATATGGG GGCGCCTAGA GAAGGAGTGA
  121 GGGCTGGATA AAGGGAGGAT CGAGGCGGGG TCGAACGAGG AGGTTCAAGG GGGAGAGACG
  181 GGGCGGATGG AGGAAGAGGA GGCGGAGGCT TAGGGTGTAC AAAGGGCTTG ACCCAGGGAG
  241 GGGGGTCAAA AGCCAAGGCT TCCCAGGTCA CGATGTAGGG GACCTGGTCT GGGTGTCCAT
  301 GCGGGCCAGG TGAAAAGACC TTGATCTTAA CCTGGGTGAT GAGGTCTCGG TTAAAGGTGC
  361 CGTCTCGCGG CCATCCGACG TTAAAGGTTG GCCATTCTGC AGAGCAGAAG GTAACCCAAC
  421 GTCTCTTCTT GACATCTACC GACTGGTTGT GAGCGATCCG CTCGACATCT TTCCAGTGAC
  481 CTAAGGTCAA ACTTAAGGGA GTGGTAACAG TCTGGCCCTA ATTTTCAGAC AAATACAGAA
  541 ACACAGTCAG ACAGAGACAA CACAGAACGA TGCTGCAGCA GACAAGACGC GCGGCGCGGC
  601 TTCGGTCCCA AACCGAAAGC AAAAATTCAG ACGGAGGCGG GAACTGTTTT AGGTTCTCGT
  661 CTCCTACCAG AACCACATAT CCCTCCTATT AGGGGGGTGC ACCAAAGAGT CCAAAACGAT
  721 CGGGATTTTT GGACTCAGGT CGGGCCACAA AAACGGNCCC CGAAGTCCCT GGGACGTCTC
  781 CCAGGGTTGC GGCCGGGTGT TCCGAACTCG TCAGTTCCAC CACGGGTCCG CCAGATACAG
  841 ANCTAGTTAG CTAACTAGTA NCGACGCANG CGCATAAAAT CAGTCATAGN NACTAGACAA
  901 TCGGANAGAN ACAGNTAAGT TGCNGGNCAG CTNANNTCCC GGNGGTGGGT CNGNGGNCCN
  961 NNGGNAGGGG NCTNCNNNTN CCNGNNNNAN NCCCNNANNN NANNNNCCCN NNNNNNNGGN
1021 NNNNNNANNN NNNNNNN
//
Primer: lacIpro-B_F (Pr0724)
Region: 3' LTR
Sequence file: RDB18738_B1C2g.seq check
>D07512B5_B1C2_3_lacIpro-B_F_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNCGNNTCN GTGAGCGAGG AAGCGGAAGA NCGCCCAATA
   61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG
  121 TAAATACTTA AGAAAAAAAA CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC
  181 GCTGACGGGT AGTCAATCAC TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC
  241 GGATGCAACT GCAAGAGGGT TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG
  301 AGGACTGGCG CCCCGAGTGA GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC
  361 GCGCGAACAG AAGCGAGAAG CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT
  421 CAGGTCCTTG GGGCACCCTG GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA
  481 CCGCATCTGG GGACCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA
  541 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA
  601 ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT
  661 TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTTCCNT GCCTTGCAAA ATGGCGTTAC
  721 TTAAGCTAGC TTGCCNAACC TACAGGTGGN GTCTTTCATT CCCCCCTTTT TCTGGAGACT
  781 AAATAAAATC TTTTANTTTA TCGTCNANGA TGCATGGGGT CGTGCGCTCC TTTNNNNCGG
  841 GCGCTGCGGG TCGTGGGGCG GGCGTCNNNA NCGGGCTTGN NNNCATGNAC CNNNNGCGCG
  901 GNCNTTCNGG CANNNCNNNG NNNNNGGNGA NNGNGANGNN NANNNNCTCG TANANNGNAN
961 NNNGNNGNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Segawa, K., Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium. J. Biol. Chem. 291 (2): 762-772 (2016). PMID 26567335. [link to RRC of NBRP]
original Segawa, K., Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure. Science 344 (6188): 1164-1168 (2014). PMID 24904167. [link to RRC of NBRP]