Retroviral expression vector of human ATP8B2.
Clone info. | Retroviral expression vector of human ATPase phospholipid transporting 8B2 (ATP8B2) tagged with FLAG at C-terminus. |
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Comment | derivation:ATP8B2, sKBM7 cell [M. Kotecki, et al. Exp. Cell Res. 252, 273-280 (1999)]. Expression was confirmed by the depositor with microscopy. |
Vector backbone | pMXs-puro (plasmid) |
Selectable markers | Ampicillin (E. coli), puromycin (mammalian cell) |
Gene/insert name | human ATP8B2 cDNA |
Depositor|Developer | Nagata, Shigekazu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Segawa, K. et al., Science, 344 (6188): 1164-1168, 2014); (Segawa, K. et al., J. Biol. Chem., 291 (2): 762-772, 2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested. |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引 用する (Segawa, K. et al., Science, 344 (6188): 1164-1168, 2014); (Segawa, K. et al., J. Biol. Chem., 291 (2): 762-772, 2016)。 利用者が教育・学術研究以外の目的で用いる場合、大阪大学と 別途 MTA を締結すること。 MTAに書く付加的使用条件: pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB18728 | human ATP8B2 | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The human ATP8B2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18728). |
Reference section:
Segawa, K., Kurata, S., Nagata, S., Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium. J. Biol. Chem. 291 (2): 762-772 (2016). PMID 26567335. [link to RRC of NBRP] |
Segawa, K., Kurata, S., Yanagihashi, Y., Brummelkamp, T.R., Matsuda, F., Nagata, S., Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure. Science 344 (6188): 1164-1168 (2014). PMID 24904167. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Segawa, K., Human Type IV P-type ATPases That Work as Plasma Membrane Phospholipid Flippases and Their Regulation by Caspase and Calcium. J. Biol. Chem. 291 (2): 762-772 (2016). PMID 26567335. [link to RRC of NBRP] |
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original | Segawa, K., Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure. Science 344 (6188): 1164-1168 (2014). PMID 24904167. [link to RRC of NBRP] |
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