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pcDNA3.1(-)-FLAG-p85 beta mutant (#RDB18723)

Expression vector of rat Pik3r2 (p85 beta) mutrant (Q223R and K660R).

Clone info. Expression vector of rat phosphoinositide-3-kinase regulatory subunit 2 (Pik3r2; p85 beta) mutrant (Q223R and K660R), tagged with FLAG at N-terminus. Expression of the mutant p85 beta gene confered strong resistance to oxidative stress in normal human diploid fibroblasts (Kano, Y.. et al., Sci. Reports, 9 (1): 12683, 2019).
Comment PCR products of p85 beta was isolated from PC12m321 cells. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1(-) (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name rat Pik3r2 cDNA
Depositor|Developer Kano, Yoshio |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.　
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

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RDB18723 pcDNA3.1(-)-FLAG-p85 beta mutant DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(-)-FLAG-p85 beta mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18723).

Reference section:

Kano, Y., Hiragami. F., Motoda, H., Akiyama, J., Koike, Y., Gomita, Y., Inoue, S., Kawaura, A., Furuta, T., Kawamura, K., C-SH2 point mutation converts p85β regulatory subunit of phosphoinositide 3-kinase to an anti-aging gene. Sci. Rep. 9 (1): 12683 (2019). PMID 31481652. [link to RRC of NBRP]
Kano, Y., Kawamura, K., Hiragami, F., Motoda, H., トランスジェニック非ヒト哺乳動物 [link to RRC of NBRP]
Kano, Y., Kawamura, K., Hiragami, F., Motoda, H., Cell production method, cell, modified pc12 cell, gene, and mutant phosphoinositide-3kinase. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18723_B1Bip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, FLAG, insert 5'
Sequence file: RDB18723_B1Bia.seq check
>D07200A2_B1Bi_1_CMV_Forward_E09_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACGGG CCCTCTAGAC
  181 TCGAGCGGCC GCCACTGTGC TGGATATCTG CAGAATTCAC CATGGACTAC AAGGACGACG
  241 ATGACAAGAT GGCAGGTGCT GAAGGCTTCC AGTACAGGGC TGTATACCCA TTCCGCCGGG
  301 AGCGGCCTGA GGACCTGGAG CTTCTTCCTG GTGACCTCCT GGTGGTGAGC CGGGTGGCTC
  361 TGCAGGCACT AGGTGTGGCT GATGGAGGAG AGCGCTGCCC ACACAACGTG GGCTGGATGC
  421 CTGGCTTCAA TGAGCGCACC CGACAGCGAG GGGACTTCCC TGGAACATAT GTGGAGTTCC
  481 TAGGACCTGT GGCTCTGGCT CGACCAGGCC CTCGCCCACG GGGGCCCCGT CCATTGCCTG
  541 CCAGGCCCTT GGATGGACCT TCTGAGTCAG GTCACACACT GGCAAGCCTG GCAGAGCAGT
  601 TCTCCCCACC TGAGCCTGCG CCCCCGATTC TGGTGAAGCT GATAGAAGCC ATTGAGCAAG
  661 CAGAGCTGGA CAGTGAATGC TACAGTAGGC CGGAGCTGCC CGCACCACGG ACAGACTGGT
  721 CCCTGAGTGA CTTGGAGCAG TGGGACCGCA CCACCTTGTA CGATGCTGTT AANGGCTTCC
  781 TGCTGGCATT GCCTGCAGCT GTGGTGACTC CTGANGCTGC ATCNGANGCT TATCGGGCGA
  841 TGCGGGAGGT TACGGGGCCT GTGGGGCTGG TGCTGGAACC CCCNACGCTG CCGTTGCACC
  901 AGGCCCNCAC TCTGCGGTTC CTGCTGNNNC NNCTGGGCCG CNNGGCCCGC ANANCANCCN
  961 CACCAGCTAC NGCNNNNNNN NCNCNGGNCN ANNGNCTTNN GGNNGCTGCN GNNGCNNGNN
1021 NNNNCGNNNN GGGNNNNNNN NNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA, insert 3' (K660R)
Sequence file: RDB18723_B1Bib.seq check
>D07200A2_B1Bi_1_bGH_rev3_F09_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNNNNNNNA CAACNGATGG CTGGCNACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGTTT AAACTTAAGC TTCAGCGTGC TGCAGGGGGC GGGCCAGGCC
  121 CGGGGGCACG CACAGGGTGT GCGAGGGTGA CGGTGAGCGC GTCATTGTGC TGCACAAGTG
  181 ATGCGTGCTG GTAGTGCAAT ACCAGCTCCT TCAGGGATCC GTACAGGTTA TAGGGCTCTG
  241 CGAAGCCAAA GCCAGTGGCT GTGCGGTAGA TGACACAGTG CCTCGTGTCG CCATCCACCA
  301 CCACAGAGCA TGCGTAACAG CCCCGCTGGC TGCTCTCCCG GATGAGGAAG GTCCCGTCTC
  361 GTTTGCCACT CAGCATCTCC TCCGCCTGTG TGCGGTTGAT CTTGCCCACG TACCACGTGC
  421 GCTCCTCATG GTGGGGGAGG GCGTCCTCAT CCTCCATCAG TGAATACTGG TCCTCAGTCT
  481 CATTCTTGAT TCCCAGCCAC TCGTTGATCT TCCTCTGTCG GGCACCTTTC TGGGTGAGCC
  541 ACACGAGGTA CTGGTCCCTG ATCTTGCGCA GCTGCATGAG ATCAGGCTTG AGGCTGTTCA
  601 TGCGCTTGTC AATCTCTCGA TTGTCCGAGG CCTGCGCCCG CAGATCCTGC TCCAGCTTCG
  661 TGCGGCTTTC ATGTATCTCC GCGATTCGAG ACTTGAGTCG CTCCGAGTTC AGCAGGATCC
  721 TCTGCATCTC CTTCTCATTG CCCTCTCGCC GGAAGCGCTC CAAATACTCC TTGCTGCACT
  781 TTTCCTGTGT CTGGNCCTGT TCTTCGAANA TCTTGATGGT CTCGTTGAAN GCCTCTATGG
  841 CTGTGCGCTT CATCTGANGC TCCTGGGANG NTCGTGTGTA NTCCTCATAN ANCTGGTCAT
  901 ACTCGCGGCT CTTGNCNTNN NNCTGCTGGT GNNANANNNT NNANCTGGGC NNCCACANNN
  961 TCTANNCTGN CCTCCNTNNN CACCTGGNNN NGNNNNNACT NNGNNNNCNN GNNANANNNN
1021 GNNNNNNNNN N
//
Primer: RDB18722#1 (Pr0982)
Region: insert mid (Q223R)
Sequence file: RDB18723_B1Bic.seq check
>D07200A2_B1Bi_1_RDB18722#1_H09_24_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCNGCCNGCN CCNCGNNNNN NCTGGTCCCT GNGTGACTTG
   61 GANCAGTGGG ACCGCACCAC CTTGTACGAT GCTGTTAAGG GCTTCCTGCT GGCATTGCCT
  121 GCAGCTGTGG TGACTCCTGA GGCTGCATCG GAGGCTTATC GGGCGATGCG GGAGGTTACG
  181 GGGCCTGTGG GGCTGGTGCT GGAACCCCCA ACGCTGCCGT TGCACCAGGC CCTCACTCTG
  241 CGGTTCCTGC TGCAGCACCT GGGCCGCGTG GCCCGCAGAG CACCCTCACC AGCTACAGCT
  301 GTCCACGCAC TGGCCAGTGC CTTCGGGCCG CTGCTGCTGC GTGCGCCTCC GCCAGGGGGC
  361 GAGGGTGATG GGAGTGAGCC TGCACCTGAC TTCCCTGTGC TGCTTCTAGA GAGGCTGGTG
  421 CAGGAGCATG TGGATGAGCA AGACACCGCC CCGCCAGCAC TGCCACCCAA ACCCTCTAAG
  481 GTAAAGCCGG CACCCACAGC CCTGGCCAAT GGAGGGAGTA CACCCTCGCT TCAGGATGCA
  541 GAGTGGTACT GGGGGGACAT TTCCAGGGAA GAGGTGAATG AGAGGCTCCG AGACACACCT
  601 GATGGTACCT TCTTAGTCCG AGATGCATCC AGCAAGATCC AAGGAGAGTA CACGCTCACC
  661 CTCAGGAAAG GCGGGAACAA CAAGCTAATC AAAGTCTTCC ACCGGGACGG TCACTATGGC
  721 TTCTCAGAGC CCCTTACCTT CTGCTCCGTG GTAGAGCTCA TCTCCCACTA CCGACATGAG
  781 TCACTGGNNC AGTACAACGC CNAGCTGGAC ACGCGCCTTC TCTACCCTGT GTCNNAGTAC
  841 CAGCAAGACC AGGTGGTGNA NGAGGACAGT GTANAGGCTG TGGGTGCCCA NCTCANGGNC
  901 TACCNNNNGC AGTACNNNGN ANAANANCCG CGAGNATGAN CAGCTCTATG ANNAATANNC
  961 ACNAACCTNC CNNNGANNTT CNNANGAANN NNACANNCNN TANANGNNNT NNNNNNANNN
1021 NNTNNANNNN NTNNNAANNA NNNNNGNNNN NNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB18723_B1Bid.seq check
>D07200A2_B1Bi_1_SV40pro_ori_F_G09_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCCTCT GCCTCTGANC TATTCCNGAA GTAGTGAGGA
   61 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC
  121 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
  181 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA
  241 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT
  301 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG
  361 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA
  421 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
  481 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG
  541 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG
  601 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC
  661 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT
  721 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
  781 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC NCGTGATATT
  841 GCTGAAGANC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG NGCTTTACGG TATCGCCGCT
  901 CCCGATTCGC AGCGCATCGC CTTCTATCGC CNNNTTGACG ANTTCTTNTG ANCNGGNNNC
961 TNGGGTNCGA ANNGNNCGAN CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kano, Y., C-SH2 point mutation converts p85β regulatory subunit of phosphoinositide 3-kinase to an anti-aging gene. Sci. Rep. 9 (1): 12683 (2019). PMID 31481652. [link to RRC of NBRP]
original Kano, Y., トランスジェニック非ヒト哺乳動物 [link to RRC of NBRP]
original Kano, Y., Cell production method, cell, modified pc12 cell, gene, and mutant phosphoinositide-3kinase. [link to RRC of NBRP]