Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1(-)-FLAG-p85 beta mutant

Expression vector of rat Pik3r2 (p85 beta) mutrant (Q223R and K660R).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18723_B1Bip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, FLAG, insert 5' Sequence file: RDB18723_B1Bia.seq
>D07200A2_B1Bi_1_CMV_Forward_E09_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGCGG TAGGCGTGTA CGGTGGGAGG    61 TCTATATAAG CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA   121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACGGG CCCTCTAGAC   181 TCGAGCGGCC GCCACTGTGC TGGATATCTG CAGAATTCAC CATGGACTAC AAGGACGACG   241 ATGACAAGAT GGCAGGTGCT GAAGGCTTCC AGTACAGGGC TGTATACCCA TTCCGCCGGG   301 AGCGGCCTGA GGACCTGGAG CTTCTTCCTG GTGACCTCCT GGTGGTGAGC CGGGTGGCTC   361 TGCAGGCACT AGGTGTGGCT GATGGAGGAG AGCGCTGCCC ACACAACGTG GGCTGGATGC   421 CTGGCTTCAA TGAGCGCACC CGACAGCGAG GGGACTTCCC TGGAACATAT GTGGAGTTCC   481 TAGGACCTGT GGCTCTGGCT CGACCAGGCC CTCGCCCACG GGGGCCCCGT CCATTGCCTG   541 CCAGGCCCTT GGATGGACCT TCTGAGTCAG GTCACACACT GGCAAGCCTG GCAGAGCAGT   601 TCTCCCCACC TGAGCCTGCG CCCCCGATTC TGGTGAAGCT GATAGAAGCC ATTGAGCAAG   661 CAGAGCTGGA CAGTGAATGC TACAGTAGGC CGGAGCTGCC CGCACCACGG ACAGACTGGT   721 CCCTGAGTGA CTTGGAGCAG TGGGACCGCA CCACCTTGTA CGATGCTGTT AANGGCTTCC   781 TGCTGGCATT GCCTGCAGCT GTGGTGACTC CTGANGCTGC ATCNGANGCT TATCGGGCGA   841 TGCGGGAGGT TACGGGGCCT GTGGGGCTGG TGCTGGAACC CCCNACGCTG CCGTTGCACC   901 AGGCCCNCAC TCTGCGGTTC CTGCTGNNNC NNCTGGGCCG CNNGGCCCGC ANANCANCCN   961 CACCAGCTAC NGCNNNNNNN NCNCNGGNCN ANNGNCTTNN GGNNGCTGCN GNNGCNNGNN 1021 NNNNCGNNNN GGGNNNNNNN NNN //
Primer: bGH_rev3 Region: bGH pA, insert 3' (K660R) Sequence file: RDB18723_B1Bib.seq
>D07200A2_B1Bi_1_bGH_rev3_F09_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNNNNNNNA CAACNGATGG CTGGCNACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGTTT AAACTTAAGC TTCAGCGTGC TGCAGGGGGC GGGCCAGGCC   121 CGGGGGCACG CACAGGGTGT GCGAGGGTGA CGGTGAGCGC GTCATTGTGC TGCACAAGTG   181 ATGCGTGCTG GTAGTGCAAT ACCAGCTCCT TCAGGGATCC GTACAGGTTA TAGGGCTCTG   241 CGAAGCCAAA GCCAGTGGCT GTGCGGTAGA TGACACAGTG CCTCGTGTCG CCATCCACCA   301 CCACAGAGCA TGCGTAACAG CCCCGCTGGC TGCTCTCCCG GATGAGGAAG GTCCCGTCTC   361 GTTTGCCACT CAGCATCTCC TCCGCCTGTG TGCGGTTGAT CTTGCCCACG TACCACGTGC   421 GCTCCTCATG GTGGGGGAGG GCGTCCTCAT CCTCCATCAG TGAATACTGG TCCTCAGTCT   481 CATTCTTGAT TCCCAGCCAC TCGTTGATCT TCCTCTGTCG GGCACCTTTC TGGGTGAGCC   541 ACACGAGGTA CTGGTCCCTG ATCTTGCGCA GCTGCATGAG ATCAGGCTTG AGGCTGTTCA   601 TGCGCTTGTC AATCTCTCGA TTGTCCGAGG CCTGCGCCCG CAGATCCTGC TCCAGCTTCG   661 TGCGGCTTTC ATGTATCTCC GCGATTCGAG ACTTGAGTCG CTCCGAGTTC AGCAGGATCC   721 TCTGCATCTC CTTCTCATTG CCCTCTCGCC GGAAGCGCTC CAAATACTCC TTGCTGCACT   781 TTTCCTGTGT CTGGNCCTGT TCTTCGAANA TCTTGATGGT CTCGTTGAAN GCCTCTATGG   841 CTGTGCGCTT CATCTGANGC TCCTGGGANG NTCGTGTGTA NTCCTCATAN ANCTGGTCAT   901 ACTCGCGGCT CTTGNCNTNN NNCTGCTGGT GNNANANNNT NNANCTGGGC NNCCACANNN   961 TCTANNCTGN CCTCCNTNNN CACCTGGNNN NGNNNNNACT NNGNNNNCNN GNNANANNNN 1021 GNNNNNNNNN N //
Primer: RDB18722#1 Region: insert mid (Q223R) Sequence file: RDB18723_B1Bic.seq
>D07200A2_B1Bi_1_RDB18722#1_H09_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNGCCNGCN CCNCGNNNNN NCTGGTCCCT GNGTGACTTG    61 GANCAGTGGG ACCGCACCAC CTTGTACGAT GCTGTTAAGG GCTTCCTGCT GGCATTGCCT   121 GCAGCTGTGG TGACTCCTGA GGCTGCATCG GAGGCTTATC GGGCGATGCG GGAGGTTACG   181 GGGCCTGTGG GGCTGGTGCT GGAACCCCCA ACGCTGCCGT TGCACCAGGC CCTCACTCTG   241 CGGTTCCTGC TGCAGCACCT GGGCCGCGTG GCCCGCAGAG CACCCTCACC AGCTACAGCT   301 GTCCACGCAC TGGCCAGTGC CTTCGGGCCG CTGCTGCTGC GTGCGCCTCC GCCAGGGGGC   361 GAGGGTGATG GGAGTGAGCC TGCACCTGAC TTCCCTGTGC TGCTTCTAGA GAGGCTGGTG   421 CAGGAGCATG TGGATGAGCA AGACACCGCC CCGCCAGCAC TGCCACCCAA ACCCTCTAAG   481 GTAAAGCCGG CACCCACAGC CCTGGCCAAT GGAGGGAGTA CACCCTCGCT TCAGGATGCA   541 GAGTGGTACT GGGGGGACAT TTCCAGGGAA GAGGTGAATG AGAGGCTCCG AGACACACCT   601 GATGGTACCT TCTTAGTCCG AGATGCATCC AGCAAGATCC AAGGAGAGTA CACGCTCACC   661 CTCAGGAAAG GCGGGAACAA CAAGCTAATC AAAGTCTTCC ACCGGGACGG TCACTATGGC   721 TTCTCAGAGC CCCTTACCTT CTGCTCCGTG GTAGAGCTCA TCTCCCACTA CCGACATGAG   781 TCACTGGNNC AGTACAACGC CNAGCTGGAC ACGCGCCTTC TCTACCCTGT GTCNNAGTAC   841 CAGCAAGACC AGGTGGTGNA NGAGGACAGT GTANAGGCTG TGGGTGCCCA NCTCANGGNC   901 TACCNNNNGC AGTACNNNGN ANAANANCCG CGAGNATGAN CAGCTCTATG ANNAATANNC   961 ACNAACCTNC CNNNGANNTT CNNANGAANN NNACANNCNN TANANGNNNT NNNNNNANNN 1021 NNTNNANNNN NTNNNAANNA NNNNNGNNNN NNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB18723_B1Bid.seq
>D07200A2_B1Bi_1_SV40pro_ori_F_G09_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCCTCT GCCTCTGANC TATTCCNGAA GTAGTGAGGA    61 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC   121 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC   181 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA   241 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT   301 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG   361 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA   421 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT   481 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG   541 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG   601 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC   661 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT   721 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC   781 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC NCGTGATATT   841 GCTGAAGANC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG NGCTTTACGG TATCGCCGCT   901 CCCGATTCGC AGCGCATCGC CTTCTATCGC CNNNTTGACG ANTTCTTNTG ANCNGGNNNC 961 TNGGGTNCGA ANNGNNCGAN CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.16

GNP_filter3_RDBDEP_html_220516.pl