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pcDNA3.1(-)-p85 beta mutant (#RDB18722)

Expression vector of rat Pik3r2 (p85 beta) mutrant (Q223R and K660R).

Clone info. Expression vector of rat phosphoinositide-3-kinase regulatory subunit 2 (Pik3r2; p85 beta) mutrant (Q223R and K660R). Expression of the mutant p85 beta gene confered strong resistance to oxidative stress in normal human diploid fibroblasts (Kano, Y.. et al., Sci. Reports, 9 (1): 12683, 2019).
Comment PCR products of p85 beta was isolated from PC12m321 cells. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1(-) (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name rat Pik3r2 cDNA
Depositor|Developer Kano, Yoshio |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

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RDB18722 pcDNA3.1(-)-p85 beta mutant DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1(-)-p85 beta mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18722).

Reference section:

Kano, Y., Hiragami. F., Motoda, H., Akiyama, J., Koike, Y., Gomita, Y., Inoue, S., Kawaura, A., Furuta, T., Kawamura, K., C-SH2 point mutation converts p85β regulatory subunit of phosphoinositide 3-kinase to an anti-aging gene. Sci. Rep. 9 (1): 12683 (2019). PMID 31481652. [link to RRC of NBRP]
Kano, Y., Kawamura, K., Hiragami, F., Motoda, H., トランスジェニック非ヒト哺乳動物 [link to RRC of NBRP]
Kano, Y., Kawamura, K., Hiragami, F., Motoda, H., Cell production method, cell, modified pc12 cell, gene, and mutant phosphoinositide-3kinase. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18722_B1Bip1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5'
Sequence file: RDB18722_B1Bia.seq check
>D07200A1_B1Bi_1_CMV_Forward_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACGGGCCCT CTAGACTCGA
  181 GCGGCCGCCA CTGTGCTGGA TATCTGCAGA ATTCGCCCTT GACTAAATGG TGGACTCTGT
  241 GACAAGTGGC TTTCAGTAAT GGCCAGCTAG GCATGTGCGG CCCCTGAGGT CGCCTTTGAC
  301 TTCTGGAAGT AGAGGTCTGC AAGCCTACCA CACAGGCCAC ACCAGCCCAT GGGGCCCCTG
  361 GAGCCCCCGG GAGCCACCTG ACTGTCCAGA CCCCCCCCCG GGCCCAGCCA TGGCAGGTGC
  421 TGAAGGCTTC CAGTACAGGG CTGTATACCC ATTCCGCCGG GAGCGGCCTG AGGACCTGGA
  481 GCTTCTTCCT GGTGACCTCC TGGTGGTGAG CCGGGTGGCT CTGCAGGCAC TAGGTGTGGC
  541 TGATGGAGGA GAGCGCTGCC CACACAACGT GGGCTGGATG CCTGGCTTCN ATGAGCGCAC
  601 CCGACAGCGA GGGGACTTCC CTGGAACATA TGTGGAGTTC CTAGGACCTG TGGCTCTGGC
  661 TCGACCAGGC CCTCGCCCAC GGGGGCCCCG TCCATTGCCT GCCAGGCCCT TGGATGGACC
  721 TTCTGAGTCA GGTCACACAC TGGCAAGCCT GGCAGANCAN TTCTCCCCAC CTGANCCTGC
  781 GCCCCCGATT CTGGTGAAGC TGATAGAAGC CATTGANCAA GCAGANCTGG ACAGTGAATG
  841 CTACAGTANG CCNGANCTGC CCGCANNNCG GACAGACTGG TCCCTGANTG ACTTGGANCA
  901 NNGGGNNCGC ACCNNNTTGN ACNANGCTGT TNNGGGNNTC NTGCNNNNNT NGCCTGCANC
961 NGTGGNGANT NNNNANGNNN NNTNGNNNNN TTANNGGGNN ANGNNNNNNN NNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA, insert 3' (K660R)
Sequence file: RDB18722_B1Bib.seq check
>D07200A1_B1Bi_1_bGH_rev3_B09_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN NACAANNGNN GGCTGGCAAC
   61 TAGAAGGCNC AGTCGAGGCT GATCAGCGGT TTAAACTTAA GCTTGGTACC GAGCTCGGAT
  121 CCACTAGTCC AGTGTGGTGG AATTCGCCCT TAGACAGACA TGGACAGGGA GGCAGGTGAG
  181 GTGGGGCGCT CAGCGTGCTG CAGGGGGCGG GCCAGGCCCG GGGGCACGCA CAGGGTGTGC
  241 GAGGGTGACG GTGAGCGCGT CATTGTGCTG CACAAGTGAT GCGTGCTGGT AGTGCAATAC
  301 CAGCTCCTTC AGGGATCCGT ACAGGTTATA GGGCTCTGCG AAGCCAAAGC CAGTGGCTGT
  361 GCGGTAGATG ACACAGTGCC TCGTGTCGCC ATCCACCACC ACAGAGCATG CGTAACAGCC
  421 CCGCTGGCTG CTCTCCCGGA TGAGGAAGGT CCCGTCTCGT TTGCCACTCA GCATCTCCTC
  481 CGCCTGTGTG CGGTTGATCT TGCCCACGTA CCACGTGCGC TCCTCATGGT GGGGGAGGGC
  541 GTCCTCATCC TCCATCAGTG AATACTGGTC CTCAGTCTCA TTCTTGATTC CCAGCCACTC
  601 GTTGATCTTC CTCTGTCGGG CACCTTTCTG GGTGAGCCAC ACGAGGTACT GGTCCCTGAT
  661 CTTGCGCAGC TGCATGAGAT CAGGCTTGAG GCTGTTCATG CGCTTGTCAA TCTCTCGATT
  721 GTCCGANGCC TGCGCCCGCA GATCCTGCTC CAGCTTCGTG CGGCTTTCAT GTATCTCCGC
  781 GATTCGAGAC TTGAGTCGCT CCGAGTTCAG CAGGATCCTC TGCATCTCCT TCTCATTGNC
  841 CTCTCGCCGG ANNCGCTCCA AATACTCCTT GCTGCACTTT TCCTGTGTCT GGCCCTGTTC
  901 TTCNAAGATC TTGATGGTCT CGTTGAANNN NCTCTATGGN TGTGCGCTTC ATCTGAAGCT
  961 NCTNGNNNGN TNNNTGTGTA TTCCNNNTAN NNCTGGNCNT ANTCNNNGNN NNNTGNNCNT
1021 NNNCTGCNNG NNNGTANNNN NTNNNNNNNN NN
//
Primer: RDB18722#1 (Pr0982)
Region: insert mid (Q223R)
Sequence file: RDB18722_B1Bic.seq check
>D07200A1_B1Bi_1_RDB18722#1_D09_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGNC TGGTCCCNGN NNNNCTTGGA
   61 GCAGTGGGAA NCGCACCACC TTGTACGATG CTGTTAAGGG CTTCCTGCTG GCATTGCCTG
  121 CAGCTGTGGT GACTCCTGAG GCTGCATCGG AGGCTTATCG GGCGATGCGG GAGGTTACGG
  181 GGCCTGTGGG GCTGGTGCTG GAACCCCCAA CGCTGCCGTT GCACCAGGCC CTCACTCTGC
  241 GGTTCCTGCT GCAGCACCTG GGCCGCGTGG CCCGCAGAGC ACCCTCACCA GCTACAGCTG
  301 TCCACGCACT GGCCAGTGCC TTCGGGCCGC TGCTGCTGCG TGCGCCTCCG CCAGGGGGCG
  361 AGGGTGATGG GAGTGAGCCT GCACCTGACT TCCCTGTGCT GCTTCTAGAG AGGCTGGTGC
  421 AGGAGCATGT GGATGAGCAA GACACCGCCC CGCCAGCACT GCCACCCAAA CCCTCTAAGG
  481 TAAAGCCGGC ACCCACAGCC CTGGCCAATG GAGGGAGTAC ACCCTCGCTT CAGGATGCAG
  541 AGTGGTACTG GGGGGACATT TCCAGGGAAG AGGTGAATGA GAGGCTCCGA GACACACCTG
  601 ATGGTACCTT CTTAGTCCGA GATGCATCCA GCAAGATCCA AGGAGAGTAC ACGCTCACCC
  661 TCAGGAAAGG CGGGAACAAC AAGCTAATCA AAGTCTTCCA CCGGGACGGT CACTATGGCT
  721 TCTCAGAGCC CCTTACCTTC TGCTCCGTGG TAGAGCTCAT CTCCCACTAC CGACATGAGT
  781 CACTGGCCCA GTACNAACGC CAAGCTGGAC ACGCGCCTTC TCTACCCTGT GNCCAAGTAC
  841 CAGCAAGACC AGGNNGTGAA AGGANGACAG NGTAGAGGCT GTGGGTGCNN NGCTCAAGGN
  901 CTACCACCNG CNNNANNNGG ANNNNANCCG CGANTATGAN NNGCTCTATG AGGNATANNN
  961 NCGANNCNTN CCNAGNANNT NNNNATGAAN CGCANNNNCN NTANNNNNCN TTCNNNNNNN
1021 NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB18722_B1Bid.seq check
>D07200A1_B1Bi_1_SV40pro_ori_F_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
  601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
  661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
  721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGANTCA TCGACTGTGG
  781 CCGGCTGGGT GTGGCGGANC GCTATCANGA NATAGCGTTG GCTACNCGTG ATATTGCTGA
  841 AGANCTTGGC GGCGAATGGG CTGANCGCTT CCTCGNGCTT TACNGTATCG CCGCTCCCGA
  901 TTCGNAGCGC ATCGCCTTCT ATCGCCTTCN TGNNNANTTC NTCTGNNCNG GNNTCTNGGG
961 GNNCGAAATG ANNNANNNNN CNNNNCCNNN NCNNGNCNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kano, Y., C-SH2 point mutation converts p85β regulatory subunit of phosphoinositide 3-kinase to an anti-aging gene. Sci. Rep. 9 (1): 12683 (2019). PMID 31481652. [link to RRC of NBRP]
original Kano, Y., トランスジェニック非ヒト哺乳動物 [link to RRC of NBRP]
original Kano, Y., Cell production method, cell, modified pc12 cell, gene, and mutant phosphoinositide-3kinase. [link to RRC of NBRP]