Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1(-)-p85 beta mutant

Expression vector of rat Pik3r2 (p85 beta) mutrant (Q223R and K660R).

Catalog number RDB18722
Resource name pcDNA3.1(-)-p85 beta mutant
Clone info. Expression vector of rat phosphoinositide-3-kinase regulatory subunit 2 (Pik3r2; p85 beta) mutrant (Q223R and K660R). Expression of the mutant p85 beta gene confered strong resistance to oxidative stress in normal human diploid fibroblasts (Kano, Y.. et al., Sci. Reports, 9 (1): 12683, 2019).
Comment PCR products of p85 beta was isolated from PC12m321 cells. Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1(-) (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name rat Pik3r2 cDNA
Depositor|Developer Kano, Yoshio |
Other clones in our bank

External Database
rat Pik3r2

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18722 pcDNA3.1(-)-p85 beta mutant DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1(-)-p85 beta mutant was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18722).

          Reference section:

          Kano, Y., Hiragami. F., Motoda, H., Akiyama, J., Koike, Y., Gomita, Y., Inoue, S., Kawaura, A., Furuta, T., Kawamura, K., C-SH2 point mutation converts p85β regulatory subunit of phosphoinositide 3-kinase to an anti-aging gene. Sci. Rep. 9 (1): 12683 (2019). PMID 31481652.
          Kano, Y., Kawamura, K., Hiragami, F., Motoda, H., トランスジェニック非ヒト哺乳動物
          Kano, Y., Kawamura, K., Hiragami, F., Motoda, H., Cell production method, cell, modified pc12 cell, gene, and mutant phosphoinositide-3kinase.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18722_B1Bip1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert 5'
          Sequence file: RDB18722_B1Bia.seq check
          >D07200A1_B1Bi_1_CMV_Forward_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
             61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
            121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACGGGCCCT CTAGACTCGA
            181 GCGGCCGCCA CTGTGCTGGA TATCTGCAGA ATTCGCCCTT GACTAAATGG TGGACTCTGT
            241 GACAAGTGGC TTTCAGTAAT GGCCAGCTAG GCATGTGCGG CCCCTGAGGT CGCCTTTGAC
            301 TTCTGGAAGT AGAGGTCTGC AAGCCTACCA CACAGGCCAC ACCAGCCCAT GGGGCCCCTG
            361 GAGCCCCCGG GAGCCACCTG ACTGTCCAGA CCCCCCCCCG GGCCCAGCCA TGGCAGGTGC
            421 TGAAGGCTTC CAGTACAGGG CTGTATACCC ATTCCGCCGG GAGCGGCCTG AGGACCTGGA
            481 GCTTCTTCCT GGTGACCTCC TGGTGGTGAG CCGGGTGGCT CTGCAGGCAC TAGGTGTGGC
            541 TGATGGAGGA GAGCGCTGCC CACACAACGT GGGCTGGATG CCTGGCTTCN ATGAGCGCAC
            601 CCGACAGCGA GGGGACTTCC CTGGAACATA TGTGGAGTTC CTAGGACCTG TGGCTCTGGC
            661 TCGACCAGGC CCTCGCCCAC GGGGGCCCCG TCCATTGCCT GCCAGGCCCT TGGATGGACC
            721 TTCTGAGTCA GGTCACACAC TGGCAAGCCT GGCAGANCAN TTCTCCCCAC CTGANCCTGC
            781 GCCCCCGATT CTGGTGAAGC TGATAGAAGC CATTGANCAA GCAGANCTGG ACAGTGAATG
            841 CTACAGTANG CCNGANCTGC CCGCANNNCG GACAGACTGG TCCCTGANTG ACTTGGANCA
            901 NNGGGNNCGC ACCNNNTTGN ACNANGCTGT TNNGGGNNTC NTGCNNNNNT NGCCTGCANC
          961 NGTGGNGANT NNNNANGNNN NNTNGNNNNN TTANNGGGNN ANGNNNNNNN NNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGH pA, insert 3' (K660R)
          Sequence file: RDB18722_B1Bib.seq check
          >D07200A1_B1Bi_1_bGH_rev3_B09_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN NACAANNGNN GGCTGGCAAC
             61 TAGAAGGCNC AGTCGAGGCT GATCAGCGGT TTAAACTTAA GCTTGGTACC GAGCTCGGAT
            121 CCACTAGTCC AGTGTGGTGG AATTCGCCCT TAGACAGACA TGGACAGGGA GGCAGGTGAG
            181 GTGGGGCGCT CAGCGTGCTG CAGGGGGCGG GCCAGGCCCG GGGGCACGCA CAGGGTGTGC
            241 GAGGGTGACG GTGAGCGCGT CATTGTGCTG CACAAGTGAT GCGTGCTGGT AGTGCAATAC
            301 CAGCTCCTTC AGGGATCCGT ACAGGTTATA GGGCTCTGCG AAGCCAAAGC CAGTGGCTGT
            361 GCGGTAGATG ACACAGTGCC TCGTGTCGCC ATCCACCACC ACAGAGCATG CGTAACAGCC
            421 CCGCTGGCTG CTCTCCCGGA TGAGGAAGGT CCCGTCTCGT TTGCCACTCA GCATCTCCTC
            481 CGCCTGTGTG CGGTTGATCT TGCCCACGTA CCACGTGCGC TCCTCATGGT GGGGGAGGGC
            541 GTCCTCATCC TCCATCAGTG AATACTGGTC CTCAGTCTCA TTCTTGATTC CCAGCCACTC
            601 GTTGATCTTC CTCTGTCGGG CACCTTTCTG GGTGAGCCAC ACGAGGTACT GGTCCCTGAT
            661 CTTGCGCAGC TGCATGAGAT CAGGCTTGAG GCTGTTCATG CGCTTGTCAA TCTCTCGATT
            721 GTCCGANGCC TGCGCCCGCA GATCCTGCTC CAGCTTCGTG CGGCTTTCAT GTATCTCCGC
            781 GATTCGAGAC TTGAGTCGCT CCGAGTTCAG CAGGATCCTC TGCATCTCCT TCTCATTGNC
            841 CTCTCGCCGG ANNCGCTCCA AATACTCCTT GCTGCACTTT TCCTGTGTCT GGCCCTGTTC
            901 TTCNAAGATC TTGATGGTCT CGTTGAANNN NCTCTATGGN TGTGCGCTTC ATCTGAAGCT
            961 NCTNGNNNGN TNNNTGTGTA TTCCNNNTAN NNCTGGNCNT ANTCNNNGNN NNNTGNNCNT
          1021 NNNCTGCNNG NNNGTANNNN NTNNNNNNNN NN
          //
          Primer: RDB18722#1 (Pr0982)
          Region: insert mid (Q223R)
          Sequence file: RDB18722_B1Bic.seq check
          >D07200A1_B1Bi_1_RDB18722#1_D09_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGNC TGGTCCCNGN NNNNCTTGGA
             61 GCAGTGGGAA NCGCACCACC TTGTACGATG CTGTTAAGGG CTTCCTGCTG GCATTGCCTG
            121 CAGCTGTGGT GACTCCTGAG GCTGCATCGG AGGCTTATCG GGCGATGCGG GAGGTTACGG
            181 GGCCTGTGGG GCTGGTGCTG GAACCCCCAA CGCTGCCGTT GCACCAGGCC CTCACTCTGC
            241 GGTTCCTGCT GCAGCACCTG GGCCGCGTGG CCCGCAGAGC ACCCTCACCA GCTACAGCTG
            301 TCCACGCACT GGCCAGTGCC TTCGGGCCGC TGCTGCTGCG TGCGCCTCCG CCAGGGGGCG
            361 AGGGTGATGG GAGTGAGCCT GCACCTGACT TCCCTGTGCT GCTTCTAGAG AGGCTGGTGC
            421 AGGAGCATGT GGATGAGCAA GACACCGCCC CGCCAGCACT GCCACCCAAA CCCTCTAAGG
            481 TAAAGCCGGC ACCCACAGCC CTGGCCAATG GAGGGAGTAC ACCCTCGCTT CAGGATGCAG
            541 AGTGGTACTG GGGGGACATT TCCAGGGAAG AGGTGAATGA GAGGCTCCGA GACACACCTG
            601 ATGGTACCTT CTTAGTCCGA GATGCATCCA GCAAGATCCA AGGAGAGTAC ACGCTCACCC
            661 TCAGGAAAGG CGGGAACAAC AAGCTAATCA AAGTCTTCCA CCGGGACGGT CACTATGGCT
            721 TCTCAGAGCC CCTTACCTTC TGCTCCGTGG TAGAGCTCAT CTCCCACTAC CGACATGAGT
            781 CACTGGCCCA GTACNAACGC CAAGCTGGAC ACGCGCCTTC TCTACCCTGT GNCCAAGTAC
            841 CAGCAAGACC AGGNNGTGAA AGGANGACAG NGTAGAGGCT GTGGGTGCNN NGCTCAAGGN
            901 CTACCACCNG CNNNANNNGG ANNNNANCCG CGANTATGAN NNGCTCTATG AGGNATANNN
            961 NCGANNCNTN CCNAGNANNT NNNNATGAAN CGCANNNNCN NTANNNNNCN TTCNNNNNNN
          1021 NNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB18722_B1Bid.seq check
          >D07200A1_B1Bi_1_SV40pro_ori_F_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
            121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
            181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
            241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
            301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
            361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
            421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
            481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
            541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
            601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
            661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
            721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGANTCA TCGACTGTGG
            781 CCGGCTGGGT GTGGCGGANC GCTATCANGA NATAGCGTTG GCTACNCGTG ATATTGCTGA
            841 AGANCTTGGC GGCGAATGGG CTGANCGCTT CCTCGNGCTT TACNGTATCG CCGCTCCCGA
            901 TTCGNAGCGC ATCGCCTTCT ATCGCCTTCN TGNNNANTTC NTCTGNNCNG GNNTCTNGGG
          961 GNNCGAAATG ANNNANNNNN CNNNNCCNNN NCNNGNCNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kano, Y., C-SH2 point mutation converts p85β regulatory subunit of phosphoinositide 3-kinase to an anti-aging gene. Sci. Rep. 9 (1): 12683 (2019). PMID 31481652.
          original Kano, Y., トランスジェニック非ヒト哺乳動物
          original Kano, Y., Cell production method, cell, modified pc12 cell, gene, and mutant phosphoinositide-3kinase.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl