Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1(-)-p85 beta mutant

Expression vector of rat Pik3r2 (p85 beta) mutrant (Q223R and K660R).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019). RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kano, Y. et al., Sci. Reports, 9 (1): 12683, 2019)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18722_B1Bip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, insert 5' Sequence file: RDB18722_B1Bia.seq
>D07200A1_B1Bi_1_CMV_Forward_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA   121 TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACGGGCCCT CTAGACTCGA   181 GCGGCCGCCA CTGTGCTGGA TATCTGCAGA ATTCGCCCTT GACTAAATGG TGGACTCTGT   241 GACAAGTGGC TTTCAGTAAT GGCCAGCTAG GCATGTGCGG CCCCTGAGGT CGCCTTTGAC   301 TTCTGGAAGT AGAGGTCTGC AAGCCTACCA CACAGGCCAC ACCAGCCCAT GGGGCCCCTG   361 GAGCCCCCGG GAGCCACCTG ACTGTCCAGA CCCCCCCCCG GGCCCAGCCA TGGCAGGTGC   421 TGAAGGCTTC CAGTACAGGG CTGTATACCC ATTCCGCCGG GAGCGGCCTG AGGACCTGGA   481 GCTTCTTCCT GGTGACCTCC TGGTGGTGAG CCGGGTGGCT CTGCAGGCAC TAGGTGTGGC   541 TGATGGAGGA GAGCGCTGCC CACACAACGT GGGCTGGATG CCTGGCTTCN ATGAGCGCAC   601 CCGACAGCGA GGGGACTTCC CTGGAACATA TGTGGAGTTC CTAGGACCTG TGGCTCTGGC   661 TCGACCAGGC CCTCGCCCAC GGGGGCCCCG TCCATTGCCT GCCAGGCCCT TGGATGGACC   721 TTCTGAGTCA GGTCACACAC TGGCAAGCCT GGCAGANCAN TTCTCCCCAC CTGANCCTGC   781 GCCCCCGATT CTGGTGAAGC TGATAGAAGC CATTGANCAA GCAGANCTGG ACAGTGAATG   841 CTACAGTANG CCNGANCTGC CCGCANNNCG GACAGACTGG TCCCTGANTG ACTTGGANCA   901 NNGGGNNCGC ACCNNNTTGN ACNANGCTGT TNNGGGNNTC NTGCNNNNNT NGCCTGCANC 961 NGTGGNGANT NNNNANGNNN NNTNGNNNNN TTANNGGGNN ANGNNNNNNN NNN //
Primer: bGH_rev3 Region: bGH pA, insert 3' (K660R) Sequence file: RDB18722_B1Bib.seq
>D07200A1_B1Bi_1_bGH_rev3_B09_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGGNN NACAANNGNN GGCTGGCAAC    61 TAGAAGGCNC AGTCGAGGCT GATCAGCGGT TTAAACTTAA GCTTGGTACC GAGCTCGGAT   121 CCACTAGTCC AGTGTGGTGG AATTCGCCCT TAGACAGACA TGGACAGGGA GGCAGGTGAG   181 GTGGGGCGCT CAGCGTGCTG CAGGGGGCGG GCCAGGCCCG GGGGCACGCA CAGGGTGTGC   241 GAGGGTGACG GTGAGCGCGT CATTGTGCTG CACAAGTGAT GCGTGCTGGT AGTGCAATAC   301 CAGCTCCTTC AGGGATCCGT ACAGGTTATA GGGCTCTGCG AAGCCAAAGC CAGTGGCTGT   361 GCGGTAGATG ACACAGTGCC TCGTGTCGCC ATCCACCACC ACAGAGCATG CGTAACAGCC   421 CCGCTGGCTG CTCTCCCGGA TGAGGAAGGT CCCGTCTCGT TTGCCACTCA GCATCTCCTC   481 CGCCTGTGTG CGGTTGATCT TGCCCACGTA CCACGTGCGC TCCTCATGGT GGGGGAGGGC   541 GTCCTCATCC TCCATCAGTG AATACTGGTC CTCAGTCTCA TTCTTGATTC CCAGCCACTC   601 GTTGATCTTC CTCTGTCGGG CACCTTTCTG GGTGAGCCAC ACGAGGTACT GGTCCCTGAT   661 CTTGCGCAGC TGCATGAGAT CAGGCTTGAG GCTGTTCATG CGCTTGTCAA TCTCTCGATT   721 GTCCGANGCC TGCGCCCGCA GATCCTGCTC CAGCTTCGTG CGGCTTTCAT GTATCTCCGC   781 GATTCGAGAC TTGAGTCGCT CCGAGTTCAG CAGGATCCTC TGCATCTCCT TCTCATTGNC   841 CTCTCGCCGG ANNCGCTCCA AATACTCCTT GCTGCACTTT TCCTGTGTCT GGCCCTGTTC   901 TTCNAAGATC TTGATGGTCT CGTTGAANNN NCTCTATGGN TGTGCGCTTC ATCTGAAGCT   961 NCTNGNNNGN TNNNTGTGTA TTCCNNNTAN NNCTGGNCNT ANTCNNNGNN NNNTGNNCNT 1021 NNNCTGCNNG NNNGTANNNN NTNNNNNNNN NN //
Primer: RDB18722#1 Region: insert mid (Q223R) Sequence file: RDB18722_B1Bic.seq
>D07200A1_B1Bi_1_RDB18722#1_D09_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGNC TGGTCCCNGN NNNNCTTGGA    61 GCAGTGGGAA NCGCACCACC TTGTACGATG CTGTTAAGGG CTTCCTGCTG GCATTGCCTG   121 CAGCTGTGGT GACTCCTGAG GCTGCATCGG AGGCTTATCG GGCGATGCGG GAGGTTACGG   181 GGCCTGTGGG GCTGGTGCTG GAACCCCCAA CGCTGCCGTT GCACCAGGCC CTCACTCTGC   241 GGTTCCTGCT GCAGCACCTG GGCCGCGTGG CCCGCAGAGC ACCCTCACCA GCTACAGCTG   301 TCCACGCACT GGCCAGTGCC TTCGGGCCGC TGCTGCTGCG TGCGCCTCCG CCAGGGGGCG   361 AGGGTGATGG GAGTGAGCCT GCACCTGACT TCCCTGTGCT GCTTCTAGAG AGGCTGGTGC   421 AGGAGCATGT GGATGAGCAA GACACCGCCC CGCCAGCACT GCCACCCAAA CCCTCTAAGG   481 TAAAGCCGGC ACCCACAGCC CTGGCCAATG GAGGGAGTAC ACCCTCGCTT CAGGATGCAG   541 AGTGGTACTG GGGGGACATT TCCAGGGAAG AGGTGAATGA GAGGCTCCGA GACACACCTG   601 ATGGTACCTT CTTAGTCCGA GATGCATCCA GCAAGATCCA AGGAGAGTAC ACGCTCACCC   661 TCAGGAAAGG CGGGAACAAC AAGCTAATCA AAGTCTTCCA CCGGGACGGT CACTATGGCT   721 TCTCAGAGCC CCTTACCTTC TGCTCCGTGG TAGAGCTCAT CTCCCACTAC CGACATGAGT   781 CACTGGCCCA GTACNAACGC CAAGCTGGAC ACGCGCCTTC TCTACCCTGT GNCCAAGTAC   841 CAGCAAGACC AGGNNGTGAA AGGANGACAG NGTAGAGGCT GTGGGTGCNN NGCTCAAGGN   901 CTACCACCNG CNNNANNNGG ANNNNANCCG CGANTATGAN NNGCTCTATG AGGNATANNN   961 NCGANNCNTN CCNAGNANNT NNNNATGAAN CGCANNNNCN NTANNNNNCN TTCNNNNNNN 1021 NNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB18722_B1Bid.seq
>D07200A1_B1Bi_1_SV40pro_ori_F_C09_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG   181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG   241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA   301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT   361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA   421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC   481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC   541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC   601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT   661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA   721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGANTCA TCGACTGTGG   781 CCGGCTGGGT GTGGCGGANC GCTATCANGA NATAGCGTTG GCTACNCGTG ATATTGCTGA   841 AGANCTTGGC GGCGAATGGG CTGANCGCTT CCTCGNGCTT TACNGTATCG CCGCTCCCGA   901 TTCGNAGCGC ATCGCCTTCT ATCGCCTTCN TGNNNANTTC NTCTGNNCNG GNNTCTNGGG 961 GNNCGAAATG ANNNANNNNN CNNNNCCNNN NCNNGNCNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.16

GNP_filter3_RDBDEP_html_220516.pl