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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL3-phPSA -430 to +12 (#RDB18677)

Luciferase reporter vector of human prostate-specific antigen (PSA) promoter (region -430 to +12).

Clone info. Luciferase reporter vector of human prostate-specific antigen (PSA) promoter (region -430 to +12).
Comment Cloned fragment: 50854482 to 50854926 (NC_000019.10). Known difference: C to T substitution at 50854757nt.
Vector backbone pGL3 (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Gene/insert name human KLK3 genomic
Depositor|Developer Negishi, Masahiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
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MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18677 pGL3-phPSA -430 to +12 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL3-phPSA -430 to +12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18677).

Reference section:

Shizu, R., Yokobori, K., Perera, L., Pedersen, L., Negishi, M., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18677_B1Aqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site,insert,luc 5'
Sequence file: RDB18677_B1Aqa.seq check
>D07007A9L_B1Aq_2_pGL4ins_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCNNANNA GGCTGTCCCC AGTGCAAGTG CNNGTGCCAG
   61 AACATTTCTC TATCGATAGG TACCGAGCTC TTACGCGTGC TAGCCCGGGC TCGAGGAATA
  121 AAAGTTCTAG TTTCTGGTCT CAGAGTGGTG CAGGGATCAG GGAGTCTCAC AATCTCCTGA
  181 GTGCTGGTGT CTTAGGGCAC ACTGGGTCTT GGAGTGCAAA GGATCTAGGC ACGTGAGGCT
  241 TTGTATGAAG AATCGGGGAT CGTACCCACC CCCTGTTTCT GTTTCATCCT GGGCGTGTCT
  301 CCTCTGCCTT TGTCCCCTAG ATGAAGTCTC CATGAGCTAC AGGGCCTGGT GCATCCAGGG
  361 TGATCTAGTA ATTGCAGAAC AGCAAGTACT AGCTCTCCCT CCCCTTCCAC AGCTCTGGGT
  421 GTGGGAGGGG GTTGTCCAGC CTCCAGCAGC ATGGGGAGGG CCTTGGTCAG CCTCTGGGTG
  481 CCAGCAGGGC AGGGGCGGAG TCCTGGGGAA TGAAGGTTTT ATAGGGCTCC TGGGGGAGGC
  541 TCCCCAGCCC CAAGCTTGGC ATTCCGGTAC TGTTGGTAAA GCCACCATGG AAGACGCCAA
  601 AAACATAAAG AAAGGCCCGG CGCCATTCTA TCCGCTGGAA GATGGAACCG CTGGAGAGCA
  661 ACTGCATAAG GCTATGAAGA GATACGCCCT GGTTCCTGGA ACAATTGCTT TTACAGATGC
  721 ACATATCGAG GTGGACATCA CTTACGCTGA GTACTTCGAA ATGTCCGTTC GGNTGGCAGA
  781 AGCTATGAAA CGATATGGGC TGAATACAAA TCACAGAATC GTCGTATGCA NTGAAAACTC
  841 TCTTCAATTC TTTATGCCGG TGTTGGGCGC GTTATTTATC GNANTTGCNG TTGCGCCCGC
  901 NAACGANATT TATAATGAAC GTGAATTGNT CNACAGTATN GNCATTTCGC ANCCTANCGT
  961 GNNGTTNGTT TCCNAAAAGG GGTTGCAAAA AATTTTNAAN GTGCAAAAAA ANNTCCNNNT
 1021 CATNCNAAAA ATTATNATCN NNNNNNCTAA AACNGNNNNA NNNNGGNNTT CNNNNNNNNN
1081 N
//
Primer: Luc_R (Pr0146)
Region: luc 5',insert,pause site,pA signal
Sequence file: RDB18677_B1Aqb.seq check
>D07007A9L_B1Aq_2_Luc_R_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNANN NNNNGNCGNN NCTCTTNNTA GCCTTATGCA GTTGCTCTCC
   61 AGCGGTTCCA TCTTCCAGCG GATAGAATGG CGCCGGGCCT TTCTTTATGT TTTTGGCGTC
  121 TTCCATGGTG GCTTTACCAA CAGTACCGGA ATGCCAAGCT TGGGGCTGGG GAGCCTCCCC
  181 CAGGAGCCCT ATAAAACCTT CATTCCCCAG GACTCCGCCC CTGCCCTGCT GGCACCCAGA
  241 GGCTGACCAA GGCCCTCCCC ATGCTGCTGG AGGCTGGACA ACCCCCTCCC ACACCCAGAG
  301 CTGTGGAAGG GGAGGGAGAG CTAGTACTTG CTGTTCTGCA ATTACTAGAT CACCCTGGAT
  361 GCACCAGGCC CTGTAGCTCA TGGAGACTTC ATCTAGGGGA CAAAGGCAGA GGAGACACGC
  421 CCAGGATGAA ACAGAAACAG GGGGTGGGTA CGATCCCCGA TTCTTCATAC AAAGCCTCAC
  481 GTGCCTAGAT CCTTTGCACT CCAAGACCCA GTGTGCCCTA AGACACCAGC ACTCAGGAGA
  541 TTGTGAGACT CCCTGATCCC TGCACCACTC TGAGACCAGA AACTAGAACT TTTATTCCTC
  601 GAGCCCGGGC TAGCACGCGT AAGAGCTCGG TACCTATCGA TAGAGAAATG TTCTGGCACC
  661 TGCACTTGCA CTGGGGACAG CCTATTTTGC TAGTTTGTTT TGTTTCGTTT TGTTTTGATG
  721 GAGAGCGTAT GTTAGTACTA TCGATTCACA CNAAAAACCN ACACACAGAT GTAATGAAAA
  781 TAAAGATATT TTATTGCGGC CGCTCNNNGT ACCTCCCGTA CCTTAATATT ACTTACTTAT
  841 CATGGTAGCT TGGGCTGNCG TAATAGCGAA NANGNCCGCA CCGATCGCCC TTNCCNACAG
  901 TTGCNCANCC TGANTGGNGA ANGNNAAANT GTAAGCGTTA ATATTTTGNN NAAATTCGCG
  961 TTAANTTTTG TTAAATCANC TCATTTTTTN ANNNNNNNGN CNAANNNGGN AAANNCCTTN
1021 NNAANCNAAA NNNNNNNCGA NANANGNNNN NNNNNN
//
Primer: SV40pA_R (Pr0882)
Region: luc 3'
Sequence file: RDB18677_B1Aqc.seq check
>D07007A9L_B1Aq_2_SV40pA_R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNTT TNNNNNNTAA AGCATTTTTT TCNCTGCATT CTAGTTGTGG
   61 TTTGTCCAAA NNNNNNNNNN NNNCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCNAN
  121 TCTAGAATTA CACGGCGATC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT
  181 TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC
  241 CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG
  301 TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT
  361 CGGGAAGACC TGCGACACCT GCGTCGAAGA TGTTGGGGTG TTGGAGCAAG ATGGATTCCA
  421 ATTCAGCGGG AGCCACCTGA TAGCCTTTGT ACTTAATCAG AGACTTCAGG CGGTCAACGA
  481 TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT
  541 TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGAC
  601 CTCTCACACA CAGTTCGCCT CTTTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA
  661 CAACCTTCGC TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT
  721 CGGGTGTAAT CAGAATAGCT GANGTAGTCT CAGTGAGCCC ATATCCTTGC CTGATACCTG
  781 NCAGANGGAA CCTCTTGGCA ACCGCTTCCN CGANTTCCTT ANAGANGGGA NCGCCACCAG
  841 AAGCAATTTC GTGTAAATTA NATAAATCGT ANTTGTCAAT CANANTGNTT TTGGCNAANA
  901 ANGGANAATA GGGNTGNNNN CAGCANCGCN CTTTGAATCT NGTAATCCTG NANNNNNNCT
  961 CANAAACNNN NCTTCTTCAA ATCTATACNN NAANANNANT CNAANTCCNN NTATNNNNAT
1021 NCNNANNGNA NNAAANNTNN CNNAANCNNG ANNGNNNNGN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Shizu, R., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [link to RRC of NBRP]