Resource data sheet
DNA Bank Top

pGL3-phPSA -430 to +12 (#RDB18677)

Luciferase reporter vector of human prostate-specific antigen (PSA) promoter (region -430 to +12).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Shizu, R., Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Luciferase reporter vector of human prostate-specific antigen (PSA) promoter (region -430 to +12).
Comment Cloned fragment: 50854482 to 50854926 (NC_000019.10). Known difference: C to T substitution at 50854757nt.
Vector backbone pGL3 (plasmid)
Size of vector backbone 4.8 kb
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human KLK3 genomic
Depositor|Developer Negishi, Masahiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB18677 pGL3-phPSA -430 to +12 DNA solution

How to cite this biological resource

Materials & Methods section:

The pGL3-phPSA -430 to +12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18677).

Reference section:

Shizu, R., Yokobori, K., Perera, L., Pedersen, L., Negishi, M., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shizu, R., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [PubMed] [Article] [RRC of NBRP]