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pcDNA3.1-FLAG-hAR (#RDB18676)

Expression vector of human androgen receptor (AR) tagged with FLAG, CMV promoter.


Drawn by SnapGene® software
Sequence (full) RDB18676hts01.seq
Publication Shizu, R., Sci. Rep. 9 (1): 16734 (2019). [link to RRC of NBRP]
Test sheet RDB18676_B3Jgp1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of human androgen receptor (AR) tagged with FLAG, CMV promoter.
Comment This clone contains a cDNA fragment corresponding to the 1130nt to 3889nt of NM_000044.6. Known differences (at least) : 8 polyglutamine tract instead of 23 polyglutamine tract after 1298nt.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human AR cDNA
Depositor|Developer Negishi, Masahiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18676 pcDNA3.1-FLAG-hAR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-FLAG-hAR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18676).

Reference section:

Shizu, R., Yokobori, K., Perera, L., Pedersen, L., Negishi, M., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shizu, R., Ligand induced dissociation of the AR homodimer precedes AR monomer translocation to the nucleus. Sci. Rep. 9 (1): 16734 (2019). PMID 31723170. [link to RRC of NBRP]