Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-FLAG-hAR

Expression vector of human androgen receptor (AR) tagged with FLAG, CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Shizu, R. et al., Sci. Rep., 9 (1): 16734, 2019).Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18676_B1Aqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: T7 pro,insert 5'(FLAG,AR 5') Sequence file: RDB18676_B1Aqa.seq
>18676_18676_B1Aq_2_CMV-Forward_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGCN NNNGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 NNNNNNNNNT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT   121 CACTATAGGG AGACCCAAGC TGGCTAGTTA AGCTTGGTAC CGAGCTCGGA TCCACTAGTC   181 CAGTGTGGTG GAATTGCCCT AAGGGCAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCC   241 ATGGACTACA AGGATGACGA TGACAAGGCA GCTGAGCAGA AACTCATCTC TGAAGAGGAT   301 CTGACTAGTG GAGAAGTGCA GTTAGGGCTG GGAAGGGTCT ACCCTCGGCC GCCGTCCAAG   361 ACCTACCGAG GAGCTTTCCA GAATCTGTTC CAGAGCGTGC GCGAAGTGAT CCAGAACCCG   421 GGCCCCAGGC ACCCAGAGGC CGCGAGCGCA GCACCTCCCG GCGCCAGTTT GCTGCTGCTG   481 CAGCAGCAGC AGCAGCAGCA GCAAGAGACT AGCCCCAGGC AGCAGCAGCA GCAGCAGGGT   541 GAGGATGGTT CTCCCCAAGC CCATCGTAGA GGCCCCACAG GCTACCTGGT CCTGGATGAG   601 GAACAGCAAC CTTCACAGCC GCAGTCGGCC CTGGAGTGCC ACCCCGAGAG AGGTTGCGTC   661 CCAGAGCCTG GAGCCGCCGT GGCCGCCAGC AAGGGGCTGC CGCAGCAGCT GCCAGCACCT   721 CCGGACGAGG ATGACTCAGC TGCCCCATCC ACGTTGTCCC TGCTGGGCCC CACTTTCCCC   781 GGCTTAAGCA GCTGCTCCGC TGANCTTAAA GACATCCTGA NCGAGGCCAG CACCATGCAA   841 CTCCTTCANN AACANCAGCA NGAANCANTA TCCGAANGCA NCANCNNNNG GNNANCNNNG   901 GANGNNTNNN GGGNCTCCCA NTTNNNCCNN NNACNNNTAC NTANGGGGCN CTTCNANNNT 961 TTNNGANNNN NNCNNNNNNN NNGNNNNANN NNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: insert 3'(AR 3') Sequence file: RDB18676_B1Aqb.seq
>18676_18676_B1Aq_2_bGH_rev3_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNGNNNNN NNGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG NTCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC GGTACGCGTA   121 GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCTT CGAACCGCGG GCCCTCTAGA   181 CTCGACACTA GTTCACTGGG TGTGGAAATA GATGGGCTTG ACTTTCCCAG AAAGGATCTT   241 GGGCACTTGC ACAGAGATGA TCTCTGCCAT CATTTCCGGA AAGTCCACGC TCACCATGTG   301 TGACTTGATT AGCAGGTCAA AAGTGAACTG ATGCAGCTCT CTCGCAATAG GCTGCACGGA   361 GTCCAGGAGC TTGGTGAGCT GGTAGAAGCG TCTTGAGCAG GATGTGGGAT TTTTTCTTTT   421 GCATGCAATG ATACGATCGA GTTCCTTGAT GTAGTTCATT CGAAGTTCAT CAAAGAATTT   481 TTGATTTTTC AGCCCATCCA CTGGAATAAT GCTGAAGAGT AGCAGTGCTT TCATGCACAG   541 GAATTCCTGG GGGGTGATTT GGAGCCATCC AAACTCTTGA GAGAGGTGCC TCATTCGGAC   601 ACACTGGCTG TACATCCGGG ACTTGTGCAT GCGGTACTCA TTGAAAACCA GATCAGGGGC   661 GAAGTAGAGC ATCCTGGAGT TGACATTGGT GAAGGATCGC CAGCCCATGG CAAACACCAT   721 GAGCCCCATC CAGGANTACT GAATGACAGC CATCTGGTCG TCCACGTGTA AGTTGCGGAA   781 GCCAGGCAAG GCCTTGGCCC ACTTGACCAC GTGTACNAGC TGTCTCTCTC CCAGTTCATT   841 GANGCTAGAN AGCAAGGNTG CAAAGGANTC GGGCTGGNTG TTGTCNNGTC CAGCACACNC   901 TACACCNNGC TNNATNNNTT NNNNGNNNTT CAGAAANANG GGNTGANNTT CNTANNNNTC 961 NNNGTGNGAN NCTNNCAGNN NNNNGNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori,NeoR Sequence file: RDB18676_B1Aqc.seq
>18676_18676_B1Aq_2_SV40pro_ori_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGCC NCCTCTGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT    61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT   121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG   181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG   241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA   301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC   361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG   421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG   481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA   541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG   601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC   661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG   721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC NTCGACTGTG   781 GNCGGCTGGG TGTGGCGGAN CGCTATCAGG ACATANCGNT GGCTANCCNT GATATTGCTG   841 AAGANCTTGN CGGCNAATGG GNNTGANCGC TTNNTCNNGC TTTACGGNAT CNCCGCTCCC   901 GANNCNCANC GNATNNNCNT NCNATCNNNT TCNTGANGAG TNNNNCTNAN CGGNNNTCNN 961 GGGTNCNNNA AANGANCNAN NNANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl