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pMM189 pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human TOMM70 N-terminal 70 a.a fused with 3xFLAG-GFP11 at C-terminus.

Catalog number RDB18665
Resource name pMM189 pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (TOMM70) N-terminal 70 a.a fused with 3xFLAG-GFP11 at C-terminus.
Vector backbone pIRESneo3 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human TOMM70 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank human TOMM70 (NCBI Gene 9868) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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Schedule A (GFP-A) [Link]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18665 pMM189 pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18665_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert, 3xFLAG, GFP11, IVS
Sequence file: RDB18665_B0Lna.seq check
>D07536B2_B0Ln_3_CMV_Forward_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNNN NGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTT GGTACCGAGC TCGGATCGAT ATCTGCGGCC
  181 TAGCTAGCGC CACCATGGCC GCGTCCAAAC CCGTGGAAGC AGCCGTCGTT GCAGCCGCTG
  241 TGCCGAGCTC TGGGTCAGGC GTGGGTGGAG GAGGAACCGC TGGTCCCGGA ACAGGCGGCC
  301 TTCCTCGGTG GCAGTTGGCC CTGGCTGTAG GGGCACCACT GCTCCTGGGA GCTGGGGCCA
  361 TCTATCTGTG GAGCCGCCAA CAGCGTCGAA GGGAGGCCAG AGGGGACTAT AAGGACCATG
  421 ATGGGGATTA CAAGGATCAC GACATTGACT ACAAGGATGA CGACGATAAA GGCGGTAGTG
  481 GCGGCGCGGC CGCCGATGGA GGGTCTGGTG GCGGATCAAC AAGTCGTGAC CACATGGTCC
  541 TTCATGAGTA CGTAAATGCT GCTGGGATTA CATAAGGATC CGCGGCCGCA TAGATAACTG
  601 ATCCAGTGTG CTGGAATTAA TTCGCTGTCT GCGAGGGCCA GCTGTTGGGG TGAGTACTCC
  661 CTCTCAAAAG CGGGCATGAC TTCTGCGCTA AGATTGTCAG TTTCCAAAAA CGAGGAGGAT
  721 TTGATATTCA CCTGGCCCGC GGTGATGCCT TTGANGGTGG CCGCGTCCAT CTGGNCAGAN
  781 NAGACAATCT TTTTGTTGTC AAGCTTGANG TGTGGCAGGC TTGAGATCTG GCCATACACT
  841 TGAGTGACAA TGACATCCAC TTTGCCTTTC TCTCCNCANG TGNNCACTCC CNGGTCCAAC
901 TGCANGNCGA GNATNNATCN NGGGCNNNNN NNNNN
//
Primer: IRES-F
Region: IRES, NeoR
Sequence file: RDB18665_B0Lnb.seq check
>D07536B2_B0Ln_3_IRES-F_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTCGANGNNN NNNNNNNTCT AGGCCCCCCG AACCNCGGGG
   61 ANGTGGTTTT CCTTTGNAAA ACACGATGAT AAGCTTGCCA CAACCCGGGA TAATTCCTGC
  121 AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
  181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
  241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
  301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
  361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
  421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
  481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
  541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
  601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
  661 GGCGCGCATG CCCGACGGCG ATGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
  721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGNTGTGGC
  781 GGANCGCTAT CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAANANC TTGGCNGCGA
  841 NTGGGCTGAN CGCTTCCTCN NGCTTTACNG TATCGCCNCT CCCNANNCNC AGCGCATCGC
  901 CTTCTATCNN NTTCTTGNNN ANNNCTTCTG ANGGGNNNNN NNCTCTANAT NNCTGNTNNT
961 NNNCANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

2021.03.26

GNP_filter3_RDBDEP_html_210308.pl