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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMM189 pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP11; location: mitochondria.

Catalog number RDB18665
Resource name pMM189 pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11
Alternative name pMM189, pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (TOMM70) N-terminal 70 a.a fused with 3xFLAG-GFP11 at C-terminus.
Vector backbone pIRESneo3 (plasmid)
Size of vector backbone 5.2 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human TOMM70 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
human TOMM70 Aequorea victoria GFP
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18665 pMM189 pIRESNeo3-TOMM70 1-70-3xFLAG-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18665_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert, 3xFLAG, GFP11, IVS
          Sequence file: RDB18665_B0Lna.seq check
          >D07536B2_B0Ln_3_CMV_Forward_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNNN NGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
            121 ATACGACTCA CTATAGGGAG ACCCAAGCTT GGTACCGAGC TCGGATCGAT ATCTGCGGCC
            181 TAGCTAGCGC CACCATGGCC GCGTCCAAAC CCGTGGAAGC AGCCGTCGTT GCAGCCGCTG
            241 TGCCGAGCTC TGGGTCAGGC GTGGGTGGAG GAGGAACCGC TGGTCCCGGA ACAGGCGGCC
            301 TTCCTCGGTG GCAGTTGGCC CTGGCTGTAG GGGCACCACT GCTCCTGGGA GCTGGGGCCA
            361 TCTATCTGTG GAGCCGCCAA CAGCGTCGAA GGGAGGCCAG AGGGGACTAT AAGGACCATG
            421 ATGGGGATTA CAAGGATCAC GACATTGACT ACAAGGATGA CGACGATAAA GGCGGTAGTG
            481 GCGGCGCGGC CGCCGATGGA GGGTCTGGTG GCGGATCAAC AAGTCGTGAC CACATGGTCC
            541 TTCATGAGTA CGTAAATGCT GCTGGGATTA CATAAGGATC CGCGGCCGCA TAGATAACTG
            601 ATCCAGTGTG CTGGAATTAA TTCGCTGTCT GCGAGGGCCA GCTGTTGGGG TGAGTACTCC
            661 CTCTCAAAAG CGGGCATGAC TTCTGCGCTA AGATTGTCAG TTTCCAAAAA CGAGGAGGAT
            721 TTGATATTCA CCTGGCCCGC GGTGATGCCT TTGANGGTGG CCGCGTCCAT CTGGNCAGAN
            781 NAGACAATCT TTTTGTTGTC AAGCTTGANG TGTGGCAGGC TTGAGATCTG GCCATACACT
            841 TGAGTGACAA TGACATCCAC TTTGCCTTTC TCTCCNCANG TGNNCACTCC CNGGTCCAAC
          901 TGCANGNCGA GNATNNATCN NGGGCNNNNN NNNNN
          //
          Primer: IRES-F
          Region: IRES, NeoR
          Sequence file: RDB18665_B0Lnb.seq check
          >D07536B2_B0Ln_3_IRES-F_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTCGANGNNN NNNNNNNTCT AGGCCCCCCG AACCNCGGGG
             61 ANGTGGTTTT CCTTTGNAAA ACACGATGAT AAGCTTGCCA CAACCCGGGA TAATTCCTGC
            121 AGCCAATATG GGATCGGCCA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG
            181 GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC
            241 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG
            301 TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
            361 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG
            421 CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT
            481 CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA
            541 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA
            601 GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
            661 GGCGCGCATG CCCGACGGCG ATGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA
            721 TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGNTGTGGC
            781 GGANCGCTAT CANGACATAG CGTTGGCTAC CCGTGATATT GCTGAANANC TTGGCNGCGA
            841 NTGGGCTGAN CGCTTCCTCN NGCTTTACNG TATCGCCNCT CCCNANNCNC AGCGCATCGC
            901 CTTCTATCNN NTTCTTGNNN ANNNCTTCTG ANGGGNNNNN NNCTCTANAT NNCTGNTNNT
          961 NNNCANNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          2022.08.11

          GNP_filter3_RDBDEP_html_220808.pl