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pMM83 pcDNA3.1-TOMM70 1-70-3xFLAG-GFP11

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human TOMM70 N-terminal 70 a.a fused with 3xFLAG-GFP11 at C-terminus.

Catalog number RDB18662
Resource name pMM83 pcDNA3.1-TOMM70 1-70-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (TOMM70) N-terminal 70 a.a fused with 3xFLAG-GFP11 at C-terminus.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human TOMM70 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Other clones in our bank human TOMM70 (NCBI Gene 9868) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18662 pMM83 pcDNA3.1-TOMM70 1-70-3xFLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18662_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: bGH_rev3
Region: bGH pA,insert (GFP11,3XFLAG,TOMM70 (1-70)),CMV pro
Sequence file: RDB18662_B0Lna.seq check
>18662_18662_B0Ln_3_bGH_rev3_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNAGNNGGC TGGCAACTAG AAGGCACAGT CGAGGCTGAT
   61 CAGCGGGTTT AAACGGGCCC TCTAGATTAT GTAATCCCAG CAGCATTTAC GTACTCATGA
  121 AGGACCATGT GGTCACGACT TGTTGATCCG CCACCAGACC CTCCATCGGC GGCCGCGCCG
  181 CCACTACCGC CTTTATCGTC GTCATCCTTG TAGTCAATGT CGTGATCCTT GTAATCCCCA
  241 TCATGGTCCT TATAGTCCCC TCTGGCCTCC CTTCGACGCT GTTGGCGGCT CCACAGATAG
  301 ATGGCCCCAG CTCCCAGGAG CAGTGGTGCC CCTACAGCCA GGGCCAACTG CCACCGAGGA
  361 AGGCCGCCTG TTCCGGGACC AGCGGTTCCT CCTCCACCCA CGCCTGACCC AGAGCTCGGC
  421 ACAGCGGCTG CAACGACGGC TGCTTCCACG GGTTTGGACG CGGCCATGGT GGCGGATCCG
  481 AGCTCGGTAC CAAGCTTAAG TTTAAACGCT AGCCAGCTTG GGTCTCCCTA TAGTGAGTCG
  541 TATTAATTTC GATAAGCCAG TAAGCAGTGG GTTCTCTAGT TAGCCAGAGA GCTCTGCTTA
  601 TATAGACCTC CNNCCGNACA CGCCTACCGC CCATTTGCGT CAATGGGGCG GANTTGTTAN
  661 NACNNTTNGG AAAGTCCCNN NGANTTTNNN GNNNNNNNAA NTNCNNNNGA NNTCNNNGGG
721 GNNNNNNNNN N
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB18662_B0Lnb.seq check
>18662_18662_B0Ln_3_SV40pro_ori_F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NCTTTTTTGG AGGCCTANGC TTTTGCAAAA AGCTCCCGGG
   61 AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
  121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
  181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
  241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA
  301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
  361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
  421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
  481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
  541 GCGAGCACGT ACTCGGANGG AAGCCGGTCT TGTCNATCAG GNNGANCTGG ACNAANAGCN
  601 TCNNGGGCTC GCGCCNGCCN AANNTGNTCG CCAGGCTNNN NNGCGCATGN CCNANNGCNA
  661 NGANNNCNNC NNGNCCCANN NNANGNCNGC TNGNNNAANA TCNNNNNNNA ANNNGNNNNN
721 NTTNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

2021.09.24

GNP_filter3_RDBDEP_html_210618.pl