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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMM83 pcDNA3.1-TOMM70 1-70-3xFLAG-GFP11

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: 3xFLAG-GFP11; location: mitochondria.

Catalog number RDB18662
Resource name pMM83 pcDNA3.1-TOMM70 1-70-3xFLAG-GFP11
Alternative name pMM83, pcDNA3.1-Tom70N-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human translocase of outer mitochondrial membrane 70 (TOMM70) N-terminal 70 a.a fused with 3xFLAG-GFP11 at C-terminus.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human TOMM70 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
Other clones in our bank
External Database
human TOMM70 Aequorea victoria GFP
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

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          RDB18662 pMM83 pcDNA3.1-TOMM70 1-70-3xFLAG-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18662_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: bGH_rev3
          Region: bGH pA,insert (GFP11,3XFLAG,TOMM70 (1-70)),CMV pro
          Sequence file: RDB18662_B0Lna.seq check
          >18662_18662_B0Ln_3_bGH_rev3_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNAGNNGGC TGGCAACTAG AAGGCACAGT CGAGGCTGAT
             61 CAGCGGGTTT AAACGGGCCC TCTAGATTAT GTAATCCCAG CAGCATTTAC GTACTCATGA
            121 AGGACCATGT GGTCACGACT TGTTGATCCG CCACCAGACC CTCCATCGGC GGCCGCGCCG
            181 CCACTACCGC CTTTATCGTC GTCATCCTTG TAGTCAATGT CGTGATCCTT GTAATCCCCA
            241 TCATGGTCCT TATAGTCCCC TCTGGCCTCC CTTCGACGCT GTTGGCGGCT CCACAGATAG
            301 ATGGCCCCAG CTCCCAGGAG CAGTGGTGCC CCTACAGCCA GGGCCAACTG CCACCGAGGA
            361 AGGCCGCCTG TTCCGGGACC AGCGGTTCCT CCTCCACCCA CGCCTGACCC AGAGCTCGGC
            421 ACAGCGGCTG CAACGACGGC TGCTTCCACG GGTTTGGACG CGGCCATGGT GGCGGATCCG
            481 AGCTCGGTAC CAAGCTTAAG TTTAAACGCT AGCCAGCTTG GGTCTCCCTA TAGTGAGTCG
            541 TATTAATTTC GATAAGCCAG TAAGCAGTGG GTTCTCTAGT TAGCCAGAGA GCTCTGCTTA
            601 TATAGACCTC CNNCCGNACA CGCCTACCGC CCATTTGCGT CAATGGGGCG GANTTGTTAN
            661 NACNNTTNGG AAAGTCCCNN NGANTTTNNN GNNNNNNNAA NTNCNNNNGA NNTCNNNGGG
          721 GNNNNNNNNN N
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18662_B0Lnb.seq check
          >18662_18662_B0Ln_3_SV40pro_ori_F_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCTTTTTTGG AGGCCTANGC TTTTGCAAAA AGCTCCCGGG
             61 AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
            121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
            181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
            241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA
            301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
            361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
            421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
            481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
            541 GCGAGCACGT ACTCGGANGG AAGCCGGTCT TGTCNATCAG GNNGANCTGG ACNAANAGCN
            601 TCNNGGGCTC GCGCCNGCCN AANNTGNTCG CCAGGCTNNN NNGCGCATGN CCNANNGCNA
            661 NGANNNCNNC NNGNCCCANN NNANGNCNGC TNGNNNAANA TCNNNNNNNA ANNNGNNNNN
          721 NTTNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          2022.08.11

          GNP_filter3_RDBDEP_html_220808.pl