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pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae PEX3 N-terminal 60 a.a fused with GFP1-10 at C-terminus.

Catalog number RDB18661
Resource name pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel Pex3p (PEX3) N-terminal 60 a.a fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, hphMX (hygromycin resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin B (yeast).
Gene/insert name S. cerevisiae PEX3 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
S. cerevisiae PEX3 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
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          RDB18661 pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB18661zzt01.seq compiled by us

          Original reference

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18661_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: f1ori_F2
          Region: GPD pro
          Sequence file: RDB18661_B0Lna.seq check
          >D07536A7_B0Ln_2_f1ori_F2_F06_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGNCGANTTC GNNCTATTGG TTAAAAAAAT GAGCTGATTT
             61 AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC ATTCGCCATT
            121 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT
            181 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC
            241 ACGACGTTGT AAAACGACGG CCAGTGAGCG CGCGTAATAC GACTCACTAT AGGGCGAATT
            301 GGGTACCGGG CCCCCCCTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTAGTTTA
            361 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
            421 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
            481 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
            541 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
            601 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
            661 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
            721 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
            781 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGNAAAAAG CTGAAAAAAA
            841 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCNTACTTGA CNNATAAGTA TATAAAGACG
            901 GTANGTATTG ATTGNAATTC TGTAAATCTA TTNCTNAAAC TTCTTAAANT CTACTTTTAT
            961 AGTTAGNCTT TTTTTNANTT TTAAAACNCC NNANNNTANT TTCNANNNAN GNNGNNNNNN
          1021 NNNN
          //
          Primer: reverse
          Region: phpMX 5'(TEF pro, HygR 5')
          Sequence file: RDB18661_B0Lnb.seq check
          >D07536A7_B0Ln_2_reverse_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNGCTGG AGCTCCNCCG CGGTGGCGGC
             61 CGCTCTAGAA CTAGTGGATC CAGTCTTGAC GTGCGCAGCT CAGGGGCATG ATGTGACTGT
            121 CGCCCGTACA TTTAGCCCAT ACATCCCCAT GTATAATCAT TTGCATCCAT ACATTTTGAT
            181 GGCCGCACGG CGCGAAGCAA AAATTACGGC TCCTCGCTGC GGACCTGCGA GCAGGGAAAC
            241 GCTCCCCTCA CAGACGCGTT GAATTGTCCC CACGCCGCGC CCCTGTAGAG AAATATAAAA
            301 GGTTAGGATT TGCCACTGAG GTTCTTCTTT CATATACTTC CTTTTAAAAT CTTGCTAGGA
            361 TACAGTTCTC ACATCACATC CGAACATAAA CAACCATGGG TAAAAAGCCT GAACTCACCG
            421 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC
            481 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC
            541 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG
            601 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA
            661 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GANNNNNAAC
            721 TGCCCGCTGT TCTGCAGCCG GTCGCGGANG CCATGGATGC GATCGCTGCG GCCGATCTTA
            781 GCCAGACNAG CGGGTTCGGN CCATTCGGAN CGCANGGAAT CGGTCAATAC ACTACATGGC
            841 GTGATTTCAT ATGCGCGATT GNTGATCCCC ATGTGTATCA CTGGCNAACT GTGNTGGANG
            901 ACACCGTCAG TGCGTCCGTC GCGCNNNNTC TCGATGANCT GANGCTTTGG GCCGAGGNNT
            961 GNCCCNAANN NCGGNACNNC GNGNNNGCNG NANTNNNNNT NCNNCNNNGN NCNGNNNGNN
           1021 NNNGGNCNNC NNNNCANNNG NNNNNNGNCT NNNNNNNNGN NNNNNTNNNG GNNNNNNNNN
          1081 N
          //
          Primer: TDH3pro_F
          Region: GPD pro 3',Pex3N(1-60),GFP1-10 5'
          Sequence file: RDB18661_B0Lnc.seq check
          >D07536A7_B0Ln_2_TDH3pro_F_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNA NNTCTNNNNN NCTATTTCTT AAACTTCTTA
             61 AATTCTACTT TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC
            121 GGATGCGGCC GCATGGCCCC AAATCAAAGA TCACGTTCGC TTCTGCAGAG ACATCGAGGA
            181 AAGGTACTCA TTTCATTGAC TGGAATAGCT GCTTTATTTA CCACAGGGTC GGTGGTAGTG
            241 TTTTTCGTGA AGAGATGGTT GTATAAACAG CAGTTACGGA TTACTGAGCA ACACTTCATC
            301 AAAGAACAGA TCGGATCCGG AGGGTCTGGT GGCGGATCAA TGGGTGGCAC TAGTATGAGC
            361 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT
            421 AATGGGCACA AATTTTCTGT CAGAGGAGAG GGTGAAGGTG ATGCTACAAT CGGAAAACTC
            481 ACACTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGCCAAC ACTTGTCACT
            541 ACTCTGACCT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG ATCACATGAA AAGGCATGAC
            601 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC GCACTATATC TTTCAAAGAT
            661 GACGGGAAAT ACAAGACGCG TGCTGTAGTC AAGTTTGAAG GNGATACCCT TGTTAATCGT
            721 ATCGAGTTAA AGGGTACTGA TTTTAAAGAA GATGGAANCA TTCTCGGACA CAAACTCGAG
            781 TACAACTTTA ACTCNCACAA TGTATACATC ACGGCAGACA AACNAAANAN NGGANTCAAA
            841 GCTAACTTCA CAGTTCGCCA CAACGTTGAA NANGGTTCCG TTNNNCTNGC ANACCNTTAT
            901 CNACAAAATA CTNCNATTGG CGATGGNCCN GTCNNTTTNN NANANNACCN TNACNTGNCG
            961 ANANNANCTG NCCNTTTCNA AANANCCCNA CGAANNTAAN NANNTNNNNA TCNNGCTNAN
          1021 NGNTNCCGNC NNNCNNCNNN NNNNNNN
          //
          Primer: Avgfp-M_F1
          Region: GFP1-10 3',CYC1 ter
          Sequence file: RDB18661_B0Lnd.seq check
          >D07536A7_B0Ln_2_Avgfp-M_F1_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNAANNNN NNNNNGCGTG CTGTAGTCAA GTTTGAAGGT
             61 GATACCCTTG TTAATCGTAT CGAGTTAAAG GGTACTGATT TTAAAGAAGA TGGAAACATT
            121 CTCGGACACA AACTCGAGTA CAACTTTAAC TCACACAATG TATACATCAC GGCAGACAAA
            181 CAAAAGAATG GAATCAAAGC TAACTTCACA GTTCGCCACA ACGTTGAAGA TGGTTCCGTT
            241 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA
            301 GACAACCATT ACCTGTCGAC ACAAACTGTC CTTTCGAAAG ATCCCAACGA AAAGTAAGAA
            361 TTCGATATCA AGCTTATCGA TACCGTCGAC CTCGAGTCAT GTAATTAGTT ATGTCACGCT
            421 TACATTCACG CCCTCCCCCC ACATCCGCTC TAACCGAAAA GGAAGGAGTT AGACAACCTG
            481 AAGTCTAGGT CCCTATTTAT TTTTTTATAG TTATGTTAGT ATTAAGAACG TTATTTATAT
            541 TTCAAATTTT TCTTTTTTTT CTGTACAGAC GCGTGTACGC ATGTAACATT ATACTGAAAA
            601 CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT TTGCGGCCGG GCAGATGATG
            661 TCGAGGCGAA AAAAAATATA AATCACGCTA ACATTTGATT AAAATAGAAC AACTACAATA
            721 TAAAAAAACT ATACAAATGA CAAGTTCTTG AAAACAAGAA TCTTTTTATT GTCNGTACTG
            781 ATTATTCCTT TGCCCTCGGA NGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGNACTT
            841 CTACACNGCC ATCGGNNCAG ACGGNCGCGC TNCTGNGGGC GANTTGTGTA CNCCCGANNG
            901 TNCCGGCTCC GGATCGGANG ANTGCGTCNC ATCNNCCCTG CGCCCAAGCT GNATCATCGA
            961 AATTGNNNTC NNCCNNNCTC TGATANAGNN GGTNNANNAN CNNNGNNNAN CATATNCGCC
          1021 CNGNANNCNN CGNNNATCNN NNNNGCNNCN GNANNNNNNN NNN
          //
          Primer: GFP-C
          Region: CYC1 ter,phpMX 3'(HygR 3')
          Sequence file: RDB18661_B0Lne.seq check
          >D07536A7_B0Ln_2_GFP-C_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTGTCCTTTC GNAAGANCCC NACGAAAAGT
             61 AAGAATTCGA TATCAAGNTT ATCGATACCG TCGACCTCGA GTCATGTAAT TAGTTATGTC
            121 ACGCTTACAT TCACGCCCTC CCCCCACATC CGCTCTAACC GAAAAGGAAG GAGTTAGACA
            181 ACCTGAAGTC TAGGTCCCTA TTTATTTTTT TATAGTTATG TTAGTATTAA GAACGTTATT
            241 TATATTTCAA ATTTTTCTTT TTTTTCTGTA CAGACGCGTG TACGCATGTA ACATTATACT
            301 GAAAACCTTG CTTGAGAAGG TTTTGGGACG CTCGAAGGCT TTAATTTGCG GCCGGGCAGA
            361 TGATGTCGAG GCGAAAAAAA ATATAAATCA CGCTAACATT TGATTAAAAT AGAACAACTA
            421 CAATATAAAA AAACTATACA AATGACAAGT TCTTGAAAAC AAGAATCTTT TTATTGTCAG
            481 TACTGATTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG
            541 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC
            601 GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CCCTGCGCCC AAGCTGCATC
            661 ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCAATGC GGAGCATATA
            721 CGCCCGGAGC CGCGGCGATC CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG
            781 CTGCTCCATA CAAGCCAACC ACGGCCTCCA NAAGAAGANG TTGGCGANCT CGTATTNGGA
            841 ATCCCCGAAC ATCGCCTCNC TCCAGTCAAT GACCGCTGTT ATGCGGCCAT TGNNNGTCAG
            901 GANATTGTNG GANCCNAAAT NCGCNNGCNN NNNGNGNCNN NANTTNNGGG NNNNNNNNNG
          961 NNNNANNGNN NTNNNNNNCA TCNNNNNNCN GNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          2021.11.02

          GNP_filter3_RDBDEP_html_211029.pl