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pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae TOM71 fused with GFP1-10 at C-terminus.

Catalog number RDB18660
Resource name pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (TOM71) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, hphMX (hygromycin resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin B (yeast).
Gene/insert name S. cerevisiae TOM71 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
S. cerevisiae TOM71 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
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          RDB18660 pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB18660zzt01.seq compiled by us

          Original reference

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18660_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: f1ori_F2
          Region: f1 ori, T7 pro, GPD pro
          Sequence file: RDB18660_B0Lna.seq check
          >D07536A6_B0Ln_1_f1ori_F2_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNT TCGGCCTATT GGTTAANAAA AATGAGCTGA
             61 TTTAACNNAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC
            121 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC
            181 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC
            241 AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG
            301 AATTGGGTAC CGGGCCCCCC CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTAG
            361 TTTATCATTA TCAATACTCG CCATTTCAAA GAATACGTAA ATAATTAATA GTAGTGATTT
            421 TCCTAACTTT ATTTAGTCAA AAAATTAGCC TTTTAATTCT GCTGTAACCC GTACATGCCC
            481 AAAATAGGGG GCGGGTTACA CAGAATATAT AACATCGTAG GTGTCTGGGT GAACAGTTTA
            541 TTCCTGGCAT CCACTAAATA TAATGGAGCC CGCTTTTTAA GCTGGCATCC AGAAAAAAAA
            601 AGAATCCCAG CACCAAAATA TTGTTTTCTT CACCAACCAT CAGTTCATAG GTCCATTCTC
            661 TTAGCGCAAC TACAGAGAAC AGGGGCACNA ACAGGCAAAA AACGGGCACA ACCTCAATGG
            721 AGTGATGCAA CCTGCCTGGA GTAAATGATG ACNCNAGGCA ATTGACCCAC GCATGTATCT
            781 ATCTCATTTT CTTACACCTT CTATTACCTT CTGCTCTCTC TGATTTGNAA AAAGCTGAAA
            841 AAAAANGTTG AAACCAGTTC CCTGAAANNA TTCCCNTACT TGACNAANAA GNATATAANN
            901 ACNGNANGNA NTGANTGNAA TTCTGNNANT CTNNTTCNTA AACTTCNTAA ATTCTACTTT
            961 TANANNNAGN CNTTTTTTTT ANNTTTAAAA NNNNNNAANN TNANNTNCNN NNNNNGNNGN
          1021 CNNNNNNNNN N
          //
          Primer: pUC-CAPbs_up_F2
          Region: T3 pro, hphMX (TEF pro, HygR)
          Sequence file: RDB18660_B0Lnb.seq check
          >D07536A6_B0Ln_1_pUC-CAPbs_up_F2_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGCTTNNN NCTTTATGCT TCCGGCTCGT ATGTTGTGTG
             61 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
            121 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
            181 CTAGAACTAG TGGATCCAGT CTTGACGTGC GCAGCTCAGG GGCATGATGT GACTGTCGCC
            241 CGTACATTTA GCCCATACAT CCCCATGTAT AATCATTTGC ATCCATACAT TTTGATGGCC
            301 GCACGGCGCG AAGCAAAAAT TACGGCTCCT CGCTGCGGAC CTGCGAGCAG GGAAACGCTC
            361 CCCTCACAGA CGCGTTGAAT TGTCCCCACG CCGCGCCCCT GTAGAGAAAT ATAAAAGGTT
            421 AGGATTTGCC ACTGAGGTTC TTCTTTCATA TACTTCCTTT TAAAATCTTG CTAGGATACA
            481 GTTCTCACAT CACATCCGAA CATAAACAAC CATGGGTAAA AAGCCTGAAC TCACCGCGAC
            541 GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC
            601 GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG
            661 GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC
            721 GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA GCCTGACCTA
            781 TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC
            841 CGCTGTTCTG CAGCCGGNCG CGGANGCCAT GGATGCGATC GCTGCNGCCG ATCTTAGNNN
            901 GANNANCGNG TTCGGNCCNT TNGGANCGCA AGGAATCGGN CAATACNCTA CNNGGCGNGA
            961 NTNCATATGN NCNANTGNTG ATCCCNNNGN GNATCACTGG NAAANTGTGN TGGNNNNNNN
          1021 NNN
          //
          Primer: TDH3pro_F
          Region: GPD pro, TOM71 5'
          Sequence file: RDB18660_B0Lnc.seq check
          >18660_18660_B0Ln_1_TDH3pro_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTTTTTTTA NTTTTAAAAC ACCAGAACTT AGTTTCGACG
             61 GATGCGGCCG CATGGCCGAA AACTCCCTCC TGAGGTTTAT CACCAAGAAC AAAGTGGCCA
            121 TCCTAGCAAC GGTCTCTGCG GGGACTGCTG CTGTGGGTGC TTATGTTTAC TACCAGCAAA
            181 TTAAGCAGCA GCAACAGCAG CAATTGAAGG GTACAAAAGA CAACCGACGT CAAAGTGAGG
            241 CCTTTGCTGG TCAAAATGAA GACGAGGCGG ACTTAAAGGA CGACGGCAGC GTCGTAAGTG
            301 GAAGTAATAA GAGGAAGAAG AAGAAGAATA AACGCAAGAG GAACAATAAG GCAAAATCGG
            361 GAGAAGGGTT TGACTATCCC TCCTTGCCCA ATGGCGAGCC TGATATTGCC CAATTGAAAG
            421 GCCTATCACC CTCCCAGAGA CAAGCGTATG CCGTGCAACT GAAGAACAGA GGTAACCACT
            481 TCTTCACTGC TAAGAATTTT AATGAGGCCA TCAAATACTA CCAGTATGCC ATTGAGCTGG
            541 ACCCGAATGA ACCAGTGTTC TATTCCAACA TATCTGCTTG TTACATTTCT ACGGGCGACT
            601 TAGAGAAGGT TATAGAGTTC ACTACAAAGG CACTTGAGAT AAAACCTGAC CATTCTAAAG
            661 CCTTGCTGAG ACGTGCATCT GCCAACGAGT CACTGGGCAA CTTCACTGAT GCTATGTTTG
            721 ATCTATCTGT TTTGTCNTTG AATGGTGACT TTGATGGANC CTCCATTNAA CCTATGCTGG
            781 AAAGAAATTT AAATAAGCAA GCAATGAAAG TACTAAATGA AAACCTATCG AANGATGAAN
            841 GCAGANGCTC GCANGTTTNG CCGNCNNACA CATCTCNNGC NNCNTTTTTT TGGGANTTTN
            901 GANTCNNACT TNNAAGTTTC NAGNGNGNNN NNCANNTNCN AACTNCNATA CNNNNNACNN
          961 NNNNNNNN
          //
          Primer: paGFP-M_R1
          Region: GFP1-10 5', TOM71 3'
          Sequence file: RDB18660_B0Lnd.seq check
          >18660_18660_B0Ln_1_paGFP-M_R1_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNNNGNNN NNNGGAAAAG CATNNNNNNC CATAGGTCAG
             61 AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA TAAATTTAAG
            121 TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA ATTTGTGCCC
            181 ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC
            241 TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC CAAGCATGCC
            301 TTTAGCCCTA TAACGAGCTA ATGTTAGCTC CATCTTTGCA CTTATGATTG GATCAGCCCT
            361 CAAACGCTTT TGTATCTTTG CAGCTTCCGC GAAGGTAGTA GCTTGAAGTT TTTCATCCAT
            421 AGTTCTTGCT AAAATTGNNN AANCNNNNNA CAATTCTATG GCCTCGTCAA TTTTTTCCAT
            481 TTGTAATTTT AGTTGTGCTA ACCCGATCTT CNCTNNGTTC ANATCGTGGA TCTNATTCNC
            541 ANGCTTTTGN CNNNNNCTTA ATGGCTGCNT TGAATTTCTC TTCNNCNNNN CTGGGTTGNA
            601 NCNTGAGAGG ANNGTCTANC TAANATANNN GCNTTCCCNN NNNNGGNNNN NANNANNNNN
          661 NNNNN
          //
          Primer: gfp_mf
          Region: GFP1-10 3', CYC1 ter
          Sequence file: RDB18660_B0Lne.seq check
          >18660_18660_B0Ln_1_gfp_mf_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAAAA CTACCNNTTC CATGGCCAAC
             61 ACTTGTCACT ACTCTGACCT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG ATCACATGAA
            121 AAGGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC GCACTATATC
            181 TTTCAAAGAT GACGGGAAAT ACAAGACGCG TGCTGTAGTC AAGTTTGAAG GTGATACCCT
            241 TGTTAATCGT ATCGAGTTAA AGGGTACTGA TTTTAAAGAA GATGGAAACA TTCTCGGACA
            301 CAAACTCGAG TACAACTTTA ACTCACACAA TGTATACATC ACGGCAGACA AACAAAAGAA
            361 TGGAATCAAA GCTAACTTCA CAGTTCGCCA CAACGTTGAA GATGGTTCCG TTCAACTAGC
            421 AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA
            481 TTACCTGTCG ACACAAACTG TCCTTTCGAA AGATCCCAAC GAAAAGTAAG AATTCGATAT
            541 CAAGCTTATC GATACCGTCG ACCTCGAGTC ATGTAATTAG TTATGTCACG CTTACATTCA
            601 CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG
            661 GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT
            721 TTTCNTTTTT TTCTGTACNG ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT
            781 GAGAANGTTT TGGGACGCTC GAANGCTTTA ATTTGCGGNN GGGCANANGA TGTCNANGCN
            841 AAAAAAAATA NNAATCACGC TAACNTTTGA TTNAAATANA ACANCTACNA TATAAAAAAA
            901 ACTATANNAA NTGANNAGTT NTNGAAANNN NNANNNNTTT TTTANTGNCN NNANNGNNTA
          961 NTNCNTTTNN NCCNNNNNNN ANNNNNNGGN NNNNNNNNTN NNNNNNNNN
          //
          Primer: GFP-C
          Region: CYC1 ter, hphMX (HygR)
          Sequence file: RDB18660_B0Lnf.seq check
          >18660_18660_B0Ln_1_GFP-C_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNACCN CNANNNATGN AATTANTTAN NNNACGCTTA
             61 CATTCACGCC CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA
            121 GTCTAGGTCC CTATTTATTT TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT
            181 CAAATTTTTC TTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC
            241 TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCGGCCGGGC AGATGATGTC
            301 GAGGCGAAAA AAAATATAAA TCACGCTAAC ATTTGATTAA AATAGAACAA CTACAATATA
            361 AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT CAGTACTGAT
            421 TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC GAGTACTTCT
            481 ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG CCCGACAGTC
            541 CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA
            601 TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG
            661 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT CTGCTGCTCC
            721 ATACAAGCCN ACCACGGCCT CCAGAANAAG ATGTTGGCGA CCTCGTATTG GGNATCCCCG
            781 AACATCGCCT CGCTCCNGTC AATGACCGCT GTTATGCGGC CATTGTCCGT CANGACATTG
            841 TTGGANCCNA AATCCGCGNG CACGANNNGN CNGANTTCNG GGNNNNCCTN NGNNCAAAGN
          901 NTCNNCTCAT NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          2021.11.17

          GNP_filter3_RDBDEP_html_211111.pl