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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX (#RDB18660)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: mitochondria.

Alternative name pYC143, pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel TOM71 (TOM71) fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, hphMX (hygromycin resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Hygromycin B (yeast).
Gene/insert name S. cerevisiae TOM71 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB18660zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18660 pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYC143 pBS-GPDp-Tom71-GFP1-10-CyC1ter-hphMX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18660).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18660_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308)
Region: f1 ori, T7 pro, GPD pro
Sequence file: RDB18660_B0Lna.seq check
>D07536A6_B0Ln_1_f1ori_F2_A07_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNT TCGGCCTATT GGTTAANAAA AATGAGCTGA
   61 TTTAACNNAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTCCATTCGC
  121 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC
  181 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC
  241 AGTCACGACG TTGTAAAACG ACGGCCAGTG AGCGCGCGTA ATACGACTCA CTATAGGGCG
  301 AATTGGGTAC CGGGCCCCCC CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTAG
  361 TTTATCATTA TCAATACTCG CCATTTCAAA GAATACGTAA ATAATTAATA GTAGTGATTT
  421 TCCTAACTTT ATTTAGTCAA AAAATTAGCC TTTTAATTCT GCTGTAACCC GTACATGCCC
  481 AAAATAGGGG GCGGGTTACA CAGAATATAT AACATCGTAG GTGTCTGGGT GAACAGTTTA
  541 TTCCTGGCAT CCACTAAATA TAATGGAGCC CGCTTTTTAA GCTGGCATCC AGAAAAAAAA
  601 AGAATCCCAG CACCAAAATA TTGTTTTCTT CACCAACCAT CAGTTCATAG GTCCATTCTC
  661 TTAGCGCAAC TACAGAGAAC AGGGGCACNA ACAGGCAAAA AACGGGCACA ACCTCAATGG
  721 AGTGATGCAA CCTGCCTGGA GTAAATGATG ACNCNAGGCA ATTGACCCAC GCATGTATCT
  781 ATCTCATTTT CTTACACCTT CTATTACCTT CTGCTCTCTC TGATTTGNAA AAAGCTGAAA
  841 AAAAANGTTG AAACCAGTTC CCTGAAANNA TTCCCNTACT TGACNAANAA GNATATAANN
  901 ACNGNANGNA NTGANTGNAA TTCTGNNANT CTNNTTCNTA AACTTCNTAA ATTCTACTTT
  961 TANANNNAGN CNTTTTTTTT ANNTTTAAAA NNNNNNAANN TNANNTNCNN NNNNNGNNGN
1021 CNNNNNNNNN N
//
Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: T3 pro, hphMX (TEF pro, HygR)
Sequence file: RDB18660_B0Lnb.seq check
>D07536A6_B0Ln_1_pUC-CAPbs_up_F2_B07_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGCTTNNN NCTTTATGCT TCCGGCTCGT ATGTTGTGTG
   61 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
  121 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT CCACCGCGGT GGCGGCCGCT
  181 CTAGAACTAG TGGATCCAGT CTTGACGTGC GCAGCTCAGG GGCATGATGT GACTGTCGCC
  241 CGTACATTTA GCCCATACAT CCCCATGTAT AATCATTTGC ATCCATACAT TTTGATGGCC
  301 GCACGGCGCG AAGCAAAAAT TACGGCTCCT CGCTGCGGAC CTGCGAGCAG GGAAACGCTC
  361 CCCTCACAGA CGCGTTGAAT TGTCCCCACG CCGCGCCCCT GTAGAGAAAT ATAAAAGGTT
  421 AGGATTTGCC ACTGAGGTTC TTCTTTCATA TACTTCCTTT TAAAATCTTG CTAGGATACA
  481 GTTCTCACAT CACATCCGAA CATAAACAAC CATGGGTAAA AAGCCTGAAC TCACCGCGAC
  541 GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC
  601 GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG
  661 GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC
  721 GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA GCCTGACCTA
  781 TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC
  841 CGCTGTTCTG CAGCCGGNCG CGGANGCCAT GGATGCGATC GCTGCNGCCG ATCTTAGNNN
  901 GANNANCGNG TTCGGNCCNT TNGGANCGCA AGGAATCGGN CAATACNCTA CNNGGCGNGA
  961 NTNCATATGN NCNANTGNTG ATCCCNNNGN GNATCACTGG NAAANTGTGN TGGNNNNNNN
1021 NNN
//
Primer: TDH3pro_F (Pr0431)
Region: GPD pro, TOM71 5'
Sequence file: RDB18660_B0Lnc.seq check
>18660_18660_B0Ln_1_TDH3pro_F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNTTTTTTTA NTTTTAAAAC ACCAGAACTT AGTTTCGACG
   61 GATGCGGCCG CATGGCCGAA AACTCCCTCC TGAGGTTTAT CACCAAGAAC AAAGTGGCCA
  121 TCCTAGCAAC GGTCTCTGCG GGGACTGCTG CTGTGGGTGC TTATGTTTAC TACCAGCAAA
  181 TTAAGCAGCA GCAACAGCAG CAATTGAAGG GTACAAAAGA CAACCGACGT CAAAGTGAGG
  241 CCTTTGCTGG TCAAAATGAA GACGAGGCGG ACTTAAAGGA CGACGGCAGC GTCGTAAGTG
  301 GAAGTAATAA GAGGAAGAAG AAGAAGAATA AACGCAAGAG GAACAATAAG GCAAAATCGG
  361 GAGAAGGGTT TGACTATCCC TCCTTGCCCA ATGGCGAGCC TGATATTGCC CAATTGAAAG
  421 GCCTATCACC CTCCCAGAGA CAAGCGTATG CCGTGCAACT GAAGAACAGA GGTAACCACT
  481 TCTTCACTGC TAAGAATTTT AATGAGGCCA TCAAATACTA CCAGTATGCC ATTGAGCTGG
  541 ACCCGAATGA ACCAGTGTTC TATTCCAACA TATCTGCTTG TTACATTTCT ACGGGCGACT
  601 TAGAGAAGGT TATAGAGTTC ACTACAAAGG CACTTGAGAT AAAACCTGAC CATTCTAAAG
  661 CCTTGCTGAG ACGTGCATCT GCCAACGAGT CACTGGGCAA CTTCACTGAT GCTATGTTTG
  721 ATCTATCTGT TTTGTCNTTG AATGGTGACT TTGATGGANC CTCCATTNAA CCTATGCTGG
  781 AAAGAAATTT AAATAAGCAA GCAATGAAAG TACTAAATGA AAACCTATCG AANGATGAAN
  841 GCAGANGCTC GCANGTTTNG CCGNCNNACA CATCTCNNGC NNCNTTTTTT TGGGANTTTN
  901 GANTCNNACT TNNAAGTTTC NAGNGNGNNN NNCANNTNCN AACTNCNATA CNNNNNACNN
961 NNNNNNNN
//
Primer: paGFP-M_R1 (Pr0893)
Region: GFP1-10 5', TOM71 3'
Sequence file: RDB18660_B0Lnd.seq check
>18660_18660_B0Ln_1_paGFP-M_R1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNNNNNGNNN NNNGGAAAAG CATNNNNNNC CATAGGTCAG
   61 AGTAGTGACA AGTGTTGGCC ATGGAACAGG TAGTTTTCCA GTAGTGCAAA TAAATTTAAG
  121 TGTGAGTTTT CCGATTGTAG CATCACCTTC ACCCTCTCCT CTGACAGAAA ATTTGTGCCC
  181 ATTAACATCA CCATCTAATT CAACAAGAAT TGGGACAACT CCAGTGAAAA GTTCTTCTCC
  241 TTTGCTCATA CTAGTGCCAC CCATTGATCC GCCACCAGAC CCTCCGGATC CAAGCATGCC
  301 TTTAGCCCTA TAACGAGCTA ATGTTAGCTC CATCTTTGCA CTTATGATTG GATCAGCCCT
  361 CAAACGCTTT TGTATCTTTG CAGCTTCCGC GAAGGTAGTA GCTTGAAGTT TTTCATCCAT
  421 AGTTCTTGCT AAAATTGNNN AANCNNNNNA CAATTCTATG GCCTCGTCAA TTTTTTCCAT
  481 TTGTAATTTT AGTTGTGCTA ACCCGATCTT CNCTNNGTTC ANATCGTGGA TCTNATTCNC
  541 ANGCTTTTGN CNNNNNCTTA ATGGCTGCNT TGAATTTCTC TTCNNCNNNN CTGGGTTGNA
  601 NCNTGAGAGG ANNGTCTANC TAANATANNN GCNTTCCCNN NNNNGGNNNN NANNANNNNN
661 NNNNN
//
Primer: gfp_mf (Pr0421)
Region: GFP1-10 3', CYC1 ter
Sequence file: RDB18660_B0Lne.seq check
>18660_18660_B0Ln_1_gfp_mf_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNAAAA CTACCNNTTC CATGGCCAAC
   61 ACTTGTCACT ACTCTGACCT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG ATCACATGAA
  121 AAGGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC GCACTATATC
  181 TTTCAAAGAT GACGGGAAAT ACAAGACGCG TGCTGTAGTC AAGTTTGAAG GTGATACCCT
  241 TGTTAATCGT ATCGAGTTAA AGGGTACTGA TTTTAAAGAA GATGGAAACA TTCTCGGACA
  301 CAAACTCGAG TACAACTTTA ACTCACACAA TGTATACATC ACGGCAGACA AACAAAAGAA
  361 TGGAATCAAA GCTAACTTCA CAGTTCGCCA CAACGTTGAA GATGGTTCCG TTCAACTAGC
  421 AGACCATTAT CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA
  481 TTACCTGTCG ACACAAACTG TCCTTTCGAA AGATCCCAAC GAAAAGTAAG AATTCGATAT
  541 CAAGCTTATC GATACCGTCG ACCTCGAGTC ATGTAATTAG TTATGTCACG CTTACATTCA
  601 CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG
  661 GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT
  721 TTTCNTTTTT TTCTGTACNG ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT
  781 GAGAANGTTT TGGGACGCTC GAANGCTTTA ATTTGCGGNN GGGCANANGA TGTCNANGCN
  841 AAAAAAAATA NNAATCACGC TAACNTTTGA TTNAAATANA ACANCTACNA TATAAAAAAA
  901 ACTATANNAA NTGANNAGTT NTNGAAANNN NNANNNNTTT TTTANTGNCN NNANNGNNTA
961 NTNCNTTTNN NCCNNNNNNN ANNNNNNGGN NNNNNNNNTN NNNNNNNNN
//
Primer: GFP-C (Pr0304)
Region: CYC1 ter, hphMX (HygR)
Sequence file: RDB18660_B0Lnf.seq check
>18660_18660_B0Ln_1_GFP-C_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNACCN CNANNNATGN AATTANTTAN NNNACGCTTA
   61 CATTCACGCC CTCCCCCCAC ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA
  121 GTCTAGGTCC CTATTTATTT TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT
  181 CAAATTTTTC TTTTTTTTCT GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC
  241 TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCGGCCGGGC AGATGATGTC
  301 GAGGCGAAAA AAAATATAAA TCACGCTAAC ATTTGATTAA AATAGAACAA CTACAATATA
  361 AAAAAACTAT ACAAATGACA AGTTCTTGAA AACAAGAATC TTTTTATTGT CAGTACTGAT
  421 TATTCCTTTG CCCTCGGACG AGTGCTGGGG CGTCGGTTTC CACTATCGGC GAGTACTTCT
  481 ACACAGCCAT CGGTCCAGAC GGCCGCGCTT CTGCGGGCGA TTTGTGTACG CCCGACAGTC
  541 CCGGCTCCGG ATCGGACGAT TGCGTCGCAT CGACCCTGCG CCCAAGCTGC ATCATCGAAA
  601 TTGCCGTCAA CCAAGCTCTG ATAGAGTTGG TCAAGACCAA TGCGGAGCAT ATACGCCCGG
  661 AGCCGCGGCG ATCCTGCAAG CTCCGGATGC CTCCGCTCGA AGTAGCGCGT CTGCTGCTCC
  721 ATACAAGCCN ACCACGGCCT CCAGAANAAG ATGTTGGCGA CCTCGTATTG GGNATCCCCG
  781 AACATCGCCT CGCTCCNGTC AATGACCGCT GTTATGCGGC CATTGTCCGT CANGACATTG
  841 TTGGANCCNA AATCCGCGNG CACGANNNGN CNGANTTCNG GGNNNNCCTN NGNNCAAAGN
901 NTCNNCTCAT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]