The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: vacuole.
Alternative name | pYC137, pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (DPP1) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, natNT2 (clonNAT resistant marker). |
Vector backbone | pBlueScript SK- (plasmid) |
Size of vector backbone | 3.0 kb |
Selectable markers | Ampicillin (E. coli), Nourseothricin (yeast). |
Gene/insert name | S. cerevisiae DPP1 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Tamura, Yasushi | |
Sequence (full) ![]() |
RDB18658zzt01.seq compiled by us |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020) |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18658 | pYC137 pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pYC137 pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18658). |
Reference section:
Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18658_B0Lnp1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: f1ori_F2 (Pr0308) Region: f1 ori, T7 pro, GPD pro Sequence file: RDB18658_B0Lna.seq ![]() |
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>D07536A4_B0Ln_2_f1ori_F2_F05_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNT NNCGANTTCG NNCTATTGGT TAAAAAAATG AGCTGATTTA 61 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCA TTCGCCATTC 121 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 181 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 241 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG 301 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATTGATAAGC TTGATATCGA ATTAGTTTAT 361 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA 421 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT 481 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT 541 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT 601 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC 661 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA 721 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC 781 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA 841 GGTTGAAACC AGTTCCCTGA AATTATTCCN CTACTTGANT AATANNNATA TNAAGANNGN 901 AGGNANNGAT TGNNATTCTG TAAATCTATT TCTTAAACTT CTNAAATTCT ACTTTTATAG 961 TTAGNCTTTT TTTTNANTTT TAAAANACNN NNNNNTNNNT TNCNANNNAN GCNGNNNNCN 1021 TNNNNNGNNN TTNCNNNTNN NTNNAANNNN NNTTNNNNNN NNNGNNNNNA NNNNNNNNNN // |
Primer: pUC-CAPbs_up_F2 (Pr0739) Region: T3 pro, natNT2 (TEF pro, NrsR) Sequence file: RDB18658_B0Lnb.seq ![]() |
>D07536A4_B0Ln_2_pUC-CAPbs_up_F2_G05_20_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTTN NNNCTTNATG CTTCCGGCTC GTATGTTGTG 61 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA 121 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG 181 CTCTAGAACT AGTGGATCCC GGGTTAATTA AGGCGCGCCA GATCTGTTTA GCTTGCCTCG 241 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT 301 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC 361 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC 421 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT 481 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC 541 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT 601 AAACAACCAT GGGTACCACT CTTGACGACA CGGCTTACCG GTACCGCACC AGTGTCCCGG 661 GGGACGCCGA GGCCATCGAG GCACTGGATG GGTCCTTCAC CACCGACACC GTCTTCCGCG 721 TCACCGCCAC CGGGGACGGC TTCACCCTGC GGGAGGTGCC GGTGGANCCG CCCCTGANNA 781 AGGTGTTCCC CGACGACGAA TCGGACGACG AATCGGANGA NGGGGAGGAN GGCGANCCGG 841 ACTCCCGGAN GTTCGTCGCG NACGGGGNNN ANNGCGACCT NNNGGGCTTC GNGNNCGNCT 901 CGNACTCCGG NNNGAANCGN CGGCTGANCG NCNANGANNT CNAGGTCGCC CCNNANCACC 961 NGGGGGCNCG GGGNCNGGCG CGCGTNGANG GGGNNNNNGA NGNNNNNNGN NNGCNANNNG 1021 GNNNNNNGNN NNCNNNGGNN NNANNNNNNN NNN // |
Primer: TDH3pro_F (Pr0431) Region: GPD pro, DPP1 5' Sequence file: RDB18658_B0Lnc.seq ![]() |
>D07536A4_B0Ln_2_TDH3pro_F_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN TGNNTGNAAT TCTGNNNNNC TATTTCTTAA ACTTCTTAAA 61 TTCTACTTTT ATAGTTAGTC TTTTTTTTAG TTTTAAAACA CCAGAACTTA GTTTCGACGG 121 ATGCGGCCGC ATGAACAGAG TTTCGTTTAT TAAAACGCCT TTCAACATAG GGGCGAAATG 181 GAGATTAGAA GATGTCTTTT TGCTCATTAT CATGATACTT CTTAACTACC CAGTGTATTA 241 CCAACAACCG TTCGAACGTC AGTTTTACAT TAACGATCTC ACTATATCGC ATCCTTATGC 301 GACAACTGAA CGTGTAAATA ACAACATGTT GTTTGTTTAT AGTTTTGTCG TGCCATCTTT 361 AACCATATTG ATAATTGGTT CCATTTTGGC CGATAGAAGA CATTTGATTT TTATTTTGTA 421 CACATCTCTC CTTGGTTTAT CACTCGCTTG GTTCAGTACG AGTTTCTTTA CAAACTTCAT 481 CAAGAATTGG ATTGGAAGAC TAAGACCAGA TTTTCTAGAT CGTTGCCAAC CTGTTGAAGG 541 CTTGCCATTG GACACTTTAT TTACTGCAAA AGATGTGTGT ACGACTAAGA ATCACGAACG 601 TCTGTTGGAT GGGTTTAGGA CAACTCCGTC AGGTCATTCA AGTGAAAGCT TTGCAGGACT 661 GGGTTATTTG TACTTCTGGC TATGTGGGCA ACTTTTGACT GAATCACCGT TGATGCCTTT 721 ATGGAGAAAA ATGGTGGCCT TTCTACCACT GTTAGGAGCT GCACTAATTG CTCTATCCAG 781 AACTCAAGAN TACAGACATC ATTTCGTCGA TGTAATTTTA GGNTCTATGT TGGGTTATAT 841 AATGGCACAC TTTTTCTACA GAAGAATCTT NCCACCCATT GATGATCCTC TTNCGTTCNA 901 ACNNTTGATG GANGATTCNN ANGNCACCCN NGNANGAAGC AGNCACCCNT CNNANGNNCC 961 CNGNANGANG AANTNCATNC TTTGTCCNAN NNANNNNATG NNNNNNNNNN // |
Primer: CYC1ter_R (Pr0022) Region: CYC1 ter, GFP11, V5 tag, DPP1 3' Sequence file: RDB18658_B0Lnd.seq ![]() |
>D07536A4_B0Ln_2_CYC1ter_R_B12_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTG GGGGGNGGGC GTGAATGTAA GCGTGACATA 61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA 121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC 181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC 241 CGGCAGAGGA TCCACTAGTC ATACCTTCAT CGGACAAAGG ATGTAATTCC TCATCCGGGA 301 TCCTCTGATG GGTGACTGCT TCCTCCAGGG TGACATCTGA ATCGTCCATC AATGGTTTGA 361 ACGGAAGAGG ATCATCAATG GGTGGGAAGA TTCTTCTGTA GAAAAAGTGT GCCATTATAT 421 AACCCAACAT AGACCCTAAA ATTACATCGA CGAAATGATG TCTGTAATCT TGAGTTCTGG 481 ATAGAGCAAT TAGTGCAGCT CCTAACAGTG GTAGAAAGGC CACCATTTTT CTCCATAAAG 541 GCATCAACGG TGATTCAGTC AAAAGTTGCC CACATAGCCA GAAGTACAAA TAACCCAGTC 601 CTGCAAAGCT TTCACTTGAA TGACCTGACG GAGTTGTCCT AAACCCATCC AACAGACGTT 661 CGTGATTCTT AGTCGTACAC ACATCTTTTG CAGTAAATAA AGTGTCCAAT GGCAAGCCTT 721 CAACAGGTTG GCAACGATCT AGAAAATCTG GTCTTAGTCT TCCAATCCAA TTCTTGATGA 781 AGTTTGTAAA GAAACTCGTA CTGAACCAAG CGAGTGATAA ACCAAGGAGA GATGTGTACA 841 AAATAAAAAT CAAATGTCTT CTATCGGCCA AAATGGAACC NATTATCAAT ATGGTTAAAG 901 ANGGNNCGAC AAAACTATAA ACAAACAACN TGTTGNTATT NNCACGTTCA GTTGNCGCAT 961 AANGNTGCNA TATANTGAGN TCGNNNATGN AAAACTNNNN TTCNNNNNNN NNN // |
Primer: CYC1ter_F (Pr0273) Region: CYC1 ter, natNT2 (ADH1 ter, NrsR) Sequence file: RDB18658_B0Lne.seq ![]() |
>D07536A4_B0Ln_2_CYC1ter_F_D05_11_ABI24.ab1 1 NNNNNNNNNN NTTNNNNNNN CTCGNNGGNT TTAATTTGCG GCCGAGCTCG ATTACAACAG 61 GTGTTGTCCT CTGAGGACAT AAAATACACA CCGAGATTCA TCAACTCATT GCTGGAGTTA 121 GCATATCTAC AATTGGGTGA AATGGGGAGC GATTTGCAGG CATTTGCTCG GCATGCCGGT 181 AGAGGTGTGG TCAATAAGAG CGACCTCATG CTATACCTGA GAAAGCAACC TGACCTACAG 241 GAAAGAGTTA CTCAAGAATA AGAATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT 301 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT 361 CGCCTCGAGA TTAGGGGCAG GGCATGCTCA TGTAGAGCGC CTGCTCGCCG TCCGAGGCGG 421 TGCCGTCGTA CAGGGCGGTG TCCAGGCCGC AGAGGGTGAA CCCCATCCGC CGGTACGCGT 481 GGATCGCCGG TGCGTTGACG TTGGTGACCT CCAGCCAGAG GTGCCCGGCG CCCCGCTCGC 541 GGGCGAACTC CGTCGCGAGC CCCATCAACG CGCGCCCGAC CCCGTGCCCC CGGTGCTCCG 601 GGGCGACCTC GATGTCCTCG ACGGTCAGCC GGCGGTTCCA GCCGGAGTAC GAGACGACCA 661 CGAAGCCCGC CAGGTCGCCG TCGTCCCCGT ACGCGACGAA CGTCCGGGAG TCCGGGTCGC 721 CGTCCTCCCC GTCGTCCGAT TCGTCGTCCG ATTCGTCGTC GGGGAACACC TTGGTCAGGG 781 GCGGGTCCAC CGGCACCTCC CGCANGGTGA AGCCGTCCCC GGTGGCGGTG ANGCGGAAGA 841 CGGTGTCGGT GGTGAAGGAN CCATCCNGNG CCTCGATGGN NTCGGCGNCC CCCGGGANAC 901 TGGTGCGGTA NCGGTAAGCC GNGNCNNCNA GANNGGTACC CATGGNTGNT TATGTNCNNN 961 NGTGATGTGA GNNNTGTATC NTANCNNGAN TTNNANNNNN GNANNNNAAA NNNNNNCNNN 1021 NNNNNNANNN NNNNNCNNTT TNNNNNTTNN NNNNNNNGGN NNNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP] |
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