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pYC137 pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 (#RDB18658)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: vacuole.

Alternative name pYC137, pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae bifunctional diacylglycerol diphosphate phosphatase/phosphatidate phosphatase (DPP1) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, natNT2 (clonNAT resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Nourseothricin (yeast).
Gene/insert name S. cerevisiae DPP1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB18658zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18658 pYC137 pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYC137 pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18658).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18658_B0Lnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308)
Region: f1 ori, T7 pro, GPD pro
Sequence file: RDB18658_B0Lna.seq check
>D07536A4_B0Ln_2_f1ori_F2_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNCGANTTCG NNCTATTGGT TAAAAAAATG AGCTGATTTA
   61 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCA TTCGCCATTC
  121 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG
  181 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
  241 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG
  301 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATTGATAAGC TTGATATCGA ATTAGTTTAT
  361 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA
  421 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT
  481 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT
  541 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT
  601 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC
  661 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA
  721 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC
  781 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA
  841 GGTTGAAACC AGTTCCCTGA AATTATTCCN CTACTTGANT AATANNNATA TNAAGANNGN
  901 AGGNANNGAT TGNNATTCTG TAAATCTATT TCTTAAACTT CTNAAATTCT ACTTTTATAG
  961 TTAGNCTTTT TTTTNANTTT TAAAANACNN NNNNNTNNNT TNCNANNNAN GCNGNNNNCN
 1021 TNNNNNGNNN TTNCNNNTNN NTNNAANNNN NNTTNNNNNN NNNGNNNNNA NNNNNNNNNN
//
Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: T3 pro, natNT2 (TEF pro, NrsR)
Sequence file: RDB18658_B0Lnb.seq check
>D07536A4_B0Ln_2_pUC-CAPbs_up_F2_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTTN NNNCTTNATG CTTCCGGCTC GTATGTTGTG
   61 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA
  121 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG
  181 CTCTAGAACT AGTGGATCCC GGGTTAATTA AGGCGCGCCA GATCTGTTTA GCTTGCCTCG
  241 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT
  301 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC
  361 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC
  421 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT
  481 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC
  541 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT
  601 AAACAACCAT GGGTACCACT CTTGACGACA CGGCTTACCG GTACCGCACC AGTGTCCCGG
  661 GGGACGCCGA GGCCATCGAG GCACTGGATG GGTCCTTCAC CACCGACACC GTCTTCCGCG
  721 TCACCGCCAC CGGGGACGGC TTCACCCTGC GGGAGGTGCC GGTGGANCCG CCCCTGANNA
  781 AGGTGTTCCC CGACGACGAA TCGGACGACG AATCGGANGA NGGGGAGGAN GGCGANCCGG
  841 ACTCCCGGAN GTTCGTCGCG NACGGGGNNN ANNGCGACCT NNNGGGCTTC GNGNNCGNCT
  901 CGNACTCCGG NNNGAANCGN CGGCTGANCG NCNANGANNT CNAGGTCGCC CCNNANCACC
  961 NGGGGGCNCG GGGNCNGGCG CGCGTNGANG GGGNNNNNGA NGNNNNNNGN NNGCNANNNG
1021 GNNNNNNGNN NNCNNNGGNN NNANNNNNNN NNN
//
Primer: TDH3pro_F (Pr0431)
Region: GPD pro, DPP1 5'
Sequence file: RDB18658_B0Lnc.seq check
>D07536A4_B0Ln_2_TDH3pro_F_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TGNNTGNAAT TCTGNNNNNC TATTTCTTAA ACTTCTTAAA
   61 TTCTACTTTT ATAGTTAGTC TTTTTTTTAG TTTTAAAACA CCAGAACTTA GTTTCGACGG
  121 ATGCGGCCGC ATGAACAGAG TTTCGTTTAT TAAAACGCCT TTCAACATAG GGGCGAAATG
  181 GAGATTAGAA GATGTCTTTT TGCTCATTAT CATGATACTT CTTAACTACC CAGTGTATTA
  241 CCAACAACCG TTCGAACGTC AGTTTTACAT TAACGATCTC ACTATATCGC ATCCTTATGC
  301 GACAACTGAA CGTGTAAATA ACAACATGTT GTTTGTTTAT AGTTTTGTCG TGCCATCTTT
  361 AACCATATTG ATAATTGGTT CCATTTTGGC CGATAGAAGA CATTTGATTT TTATTTTGTA
  421 CACATCTCTC CTTGGTTTAT CACTCGCTTG GTTCAGTACG AGTTTCTTTA CAAACTTCAT
  481 CAAGAATTGG ATTGGAAGAC TAAGACCAGA TTTTCTAGAT CGTTGCCAAC CTGTTGAAGG
  541 CTTGCCATTG GACACTTTAT TTACTGCAAA AGATGTGTGT ACGACTAAGA ATCACGAACG
  601 TCTGTTGGAT GGGTTTAGGA CAACTCCGTC AGGTCATTCA AGTGAAAGCT TTGCAGGACT
  661 GGGTTATTTG TACTTCTGGC TATGTGGGCA ACTTTTGACT GAATCACCGT TGATGCCTTT
  721 ATGGAGAAAA ATGGTGGCCT TTCTACCACT GTTAGGAGCT GCACTAATTG CTCTATCCAG
  781 AACTCAAGAN TACAGACATC ATTTCGTCGA TGTAATTTTA GGNTCTATGT TGGGTTATAT
  841 AATGGCACAC TTTTTCTACA GAAGAATCTT NCCACCCATT GATGATCCTC TTNCGTTCNA
  901 ACNNTTGATG GANGATTCNN ANGNCACCCN NGNANGAAGC AGNCACCCNT CNNANGNNCC
961 CNGNANGANG AANTNCATNC TTTGTCCNAN NNANNNNATG NNNNNNNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: CYC1 ter, GFP11, V5 tag, DPP1 3'
Sequence file: RDB18658_B0Lnd.seq check
>D07536A4_B0Ln_2_CYC1ter_R_B12_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTG GGGGGNGGGC GTGAATGTAA GCGTGACATA
   61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA
  121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC
  181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC
  241 CGGCAGAGGA TCCACTAGTC ATACCTTCAT CGGACAAAGG ATGTAATTCC TCATCCGGGA
  301 TCCTCTGATG GGTGACTGCT TCCTCCAGGG TGACATCTGA ATCGTCCATC AATGGTTTGA
  361 ACGGAAGAGG ATCATCAATG GGTGGGAAGA TTCTTCTGTA GAAAAAGTGT GCCATTATAT
  421 AACCCAACAT AGACCCTAAA ATTACATCGA CGAAATGATG TCTGTAATCT TGAGTTCTGG
  481 ATAGAGCAAT TAGTGCAGCT CCTAACAGTG GTAGAAAGGC CACCATTTTT CTCCATAAAG
  541 GCATCAACGG TGATTCAGTC AAAAGTTGCC CACATAGCCA GAAGTACAAA TAACCCAGTC
  601 CTGCAAAGCT TTCACTTGAA TGACCTGACG GAGTTGTCCT AAACCCATCC AACAGACGTT
  661 CGTGATTCTT AGTCGTACAC ACATCTTTTG CAGTAAATAA AGTGTCCAAT GGCAAGCCTT
  721 CAACAGGTTG GCAACGATCT AGAAAATCTG GTCTTAGTCT TCCAATCCAA TTCTTGATGA
  781 AGTTTGTAAA GAAACTCGTA CTGAACCAAG CGAGTGATAA ACCAAGGAGA GATGTGTACA
  841 AAATAAAAAT CAAATGTCTT CTATCGGCCA AAATGGAACC NATTATCAAT ATGGTTAAAG
  901 ANGGNNCGAC AAAACTATAA ACAAACAACN TGTTGNTATT NNCACGTTCA GTTGNCGCAT
961 AANGNTGCNA TATANTGAGN TCGNNNATGN AAAACTNNNN TTCNNNNNNN NNN
//
Primer: CYC1ter_F (Pr0273)
Region: CYC1 ter, natNT2 (ADH1 ter, NrsR)
Sequence file: RDB18658_B0Lne.seq check
>D07536A4_B0Ln_2_CYC1ter_F_D05_11_ABI24.ab1
    1 NNNNNNNNNN NTTNNNNNNN CTCGNNGGNT TTAATTTGCG GCCGAGCTCG ATTACAACAG
   61 GTGTTGTCCT CTGAGGACAT AAAATACACA CCGAGATTCA TCAACTCATT GCTGGAGTTA
  121 GCATATCTAC AATTGGGTGA AATGGGGAGC GATTTGCAGG CATTTGCTCG GCATGCCGGT
  181 AGAGGTGTGG TCAATAAGAG CGACCTCATG CTATACCTGA GAAAGCAACC TGACCTACAG
  241 GAAAGAGTTA CTCAAGAATA AGAATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT
  301 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT
  361 CGCCTCGAGA TTAGGGGCAG GGCATGCTCA TGTAGAGCGC CTGCTCGCCG TCCGAGGCGG
  421 TGCCGTCGTA CAGGGCGGTG TCCAGGCCGC AGAGGGTGAA CCCCATCCGC CGGTACGCGT
  481 GGATCGCCGG TGCGTTGACG TTGGTGACCT CCAGCCAGAG GTGCCCGGCG CCCCGCTCGC
  541 GGGCGAACTC CGTCGCGAGC CCCATCAACG CGCGCCCGAC CCCGTGCCCC CGGTGCTCCG
  601 GGGCGACCTC GATGTCCTCG ACGGTCAGCC GGCGGTTCCA GCCGGAGTAC GAGACGACCA
  661 CGAAGCCCGC CAGGTCGCCG TCGTCCCCGT ACGCGACGAA CGTCCGGGAG TCCGGGTCGC
  721 CGTCCTCCCC GTCGTCCGAT TCGTCGTCCG ATTCGTCGTC GGGGAACACC TTGGTCAGGG
  781 GCGGGTCCAC CGGCACCTCC CGCANGGTGA AGCCGTCCCC GGTGGCGGTG ANGCGGAAGA
  841 CGGTGTCGGT GGTGAAGGAN CCATCCNGNG CCTCGATGGN NTCGGCGNCC CCCGGGANAC
  901 TGGTGCGGTA NCGGTAAGCC GNGNCNNCNA GANNGGTACC CATGGNTGNT TATGTNCNNN
  961 NGTGATGTGA GNNNTGTATC NTANCNNGAN TTNNANNNNN GNANNNNAAA NNNNNNCNNN
1021 NNNNNNANNN NNNNNCNNTT TNNNNNTTNN NNNNNNNGGN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]