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pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2

The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae Erg6 fused with V5-GFP11 at C-terminus.

Catalog number RDB18657
Resource name pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, natNT2 (clonNAT resistant marker).
Vector backbone pBlueScript SK- (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin (E. coli), Nourseothricin (yeast).
Gene/insert name S. cerevisiae ERG6 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
S. cerevisiae ERG6 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
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          RDB18657 pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB18657zzt01.seq compiled by us

          Original reference

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18657_B0Lnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: f1ori_F2
          Region: f1 ori, T7 pro, GPD pro
          Sequence file: RDB18657_B0Lna.seq check
          >D07536A3_B0Ln_2_f1ori_F2_F09_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTTCGNNCT NTTGNNNNNN NNAATGAGCT
             61 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTCCATTCG
            121 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
            181 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC
            241 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC
            301 GAATTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC
            361 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT
            421 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC
            481 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT
            541 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA
            601 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT
            661 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCNNTG
            721 GAGTGATGCA NCCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC
            781 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGANTTGNA AAAAGCTGAA
            841 AAAAAAGGTT GAAACCNGTT CCCTGAAATT ATNCCCCTAC TTGANTAATA AGNATANNAA
            901 AGACNGNAGG NATTGATTGN ANTNNGNAAA TCTNTTNCTT AAACNTCTTA AATTCNACTT
          961 TTATAGNTAN NCTTTTTTTT ANNTTNNAAA NNNNNNNNNN
          //
          Primer: pUC-CAPbs_up_F2
          Region: T3 pro, natNT2 (TEF pro, NrsR)
          Sequence file: RDB18657_B0Lnb.seq check
          >D07536A3_B0Ln_2_pUC-CAPbs_up_F2_G04_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNNTTTATG CTTCCGGCTC GTATGTTGTG
             61 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA
            121 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG
            181 CTCTAGAACT AGTGGATCCC GGGTTAATTA AGGCGCGCCA GATCTGTTTA GCTTGCCTCG
            241 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT
            301 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC
            361 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC
            421 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT
            481 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC
            541 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT
            601 AAACAACCAT GGGTACCACT CTTGACGACA CGGCTTACCG GTACCGCACC AGTGTCCCGG
            661 GGGACGCCGA GGCCATCGAG GCACTGGATG GGTCCTTCAC CACCGACACC GTCTTCCGCG
            721 TCACCGCCAC CGGGGANGGC TTCACCCTGC GGGAGGTGNC GGTGGACCCG CCCCTGANCA
            781 AGGTGTTCCC CGACGACGAA TCGGANGANG AATCGGACGA NGGGGANGAN GGCGACNCGG
            841 ANTNCCGGAN GTNCGTCGCG TACNGGGANG ANGGCNACCT GNCGGGCTTC GTGGNCGTCT
            901 CGTACTCCGG CTGGNACCGN CGGCTGANCG TCNAGGANAT CGAGGTCGCC CCNNANCNNC
            961 NGGGGGCNNG GGGNCNGGCG CGCNTTNNNG GGGNTCGCGA NNNANNNCGN CNGCNANNGG
          1021 GGCNNNNNNN NNN
          //
          Primer: TDH3pro_F
          Region: GPD pro, Erg6 5'
          Sequence file: RDB18657_B0Lnc.seq check
          >D07536A3_B0Ln_2_TDH3pro_F_E04_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNTGNNNTTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT
             61 CTACTTTTAT AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT
            121 GCGGCCGCAT GAGTGAAACA GAATTGAGAA AAAGACAGGC CCAATTCACT AGGGAGTTAC
            181 ATGGTGATGA TATTGGTAAA AAGACAGGTT TGAGTGCATT GATGTCGAAG AACAACTCTG
            241 CCCAAAAGGA AGCCGTTCAG AAGTACTTGA GAAATTGGGA TGGTAGAACC GATAAAGATG
            301 CCGAAGAACG TCGTCTTGAG GATTATAATG AAGCCACACA TTCCTACTAT AACGTCGTTA
            361 CAGATTTCTA TGAATATGGT TGGGGTTCCT CTTTCCATTT CAGCAGATTT TATAAAGGTG
            421 AGAGTTTCGC TGCCTCGATA GCAAGACATG AACATTATTT AGCTTACAAG GCTGGTATTC
            481 AAAGAGGCGA TTTAGTTCTC GACGTTGGTT GTGGTGTTGG GGGCCCAGCA AGAGAGATTG
            541 CAAGATTTAC CGGTTGTAAC GTCATCGGTC TAAACAATAA CGATTACCAA ATTGCCAAGG
            601 CAAAATATTA CGCTAAAAAA TACAATTTGA GTGACCAAAT GGACTTTGTA AAGGGTGATT
            661 TCATGAAAAT GGATTTCGAA GAAAACACTT TCGACAAAGT TTATGCAATT GAGGCCACAT
            721 GTCACGCTCC AAAATTAGAA GGTGTATACA GCGAAATCTA CAAGGTTTTG AAACCGGGTG
            781 GTACCTTTGC TGTTTACNAA TGGGTAATGA CTGATAAATA TGACGAAAAC AATCCTGANC
            841 ATAGAAAGAN CGCTTATGAA ATTGANNTAN GTGATGGNAT CCCNAAGATG TTCCNNGTCG
            901 ACGNGGCTAG GAAAGCATTG AAGAACNGNG NNTTCNAANN CCTCNTNAGC GANNNNCTGN
            961 NGNANNNGNT GANGAAANNC CNNNNNNNNN CCNNNTNNNN NGNNGANNNN NNGTANGTNN
           1021 AAAACTNNNC TNANNTNNNN NNNNNTTTTN NNNNNNTNNN NNNNNNGGNN NNNNNNTTNN
          1081 NNNNNNNN
          //
          Primer: CYC1ter_R
          Region: CYC1 ter, GFP11, V5 tag, Erg6 3'
          Sequence file: RDB18657_B0Lnd.seq check
          >D07536A3_B0Ln_2_CYC1ter_R_C04_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NAGNGGNNGT GGGGGGAGGG CGTGAATGTA AGCGTGACAT
             61 AACTAATTAC ATGACTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCTTAGGTA
            121 ATGCCTGCAG CATTGACATA TTCGTGTAGA ACCATATGAT CTCTGCTTGT GGAACCACCA
            181 CCACTTCCAC CAGTTGAGTC TAAGCCCAAA AGAGGGTTTG GTATGGGTTT CCCAGAACCT
            241 CCGGCAGAGG ATCCTTGAGT TGCTTCTTGG GAAGTTTGGG AGGGGGTTTC GGCGTTTTCT
            301 GGCTTCCTAG CGACGAAAAG CATCATTGGA GTGAATAACT TGGACTTACC ACCGGCAACT
            361 AAACCAACCG CAGCATTTTC TAGAGCAGCA GTAACTTCCT TGGAACCTTC TGGGGCTAGA
            421 CCTAATTTTT CCATTACAGT AACCATTGCT GTAGTAAATT GTCTACCCAA GTAAGAAGTT
            481 CTGAAAAATG TGGCCAAATT AGCTAAGTTT TGAACGTACT TCCACTCACC AGTTAATGGG
            541 TAATACCAAG GGATTTCATC ATCATTGTCC GCCAGGTCTT CGCTAACGAG GACTTCGAAA
            601 CCACAGTTCT TCAATGCTTT CCTAGCCACG TCGACATGGA ACATCTTTGG GATACCATCA
            661 CCTAGTTCAA TTTCATAAGC GATCTTTCTA TGTTCAGGAT TGTTTTCGTC ATATTTATCA
            721 GTCATTACCC ATTCGTAAAC AGCAAAGGTA CCACCCGGTT TCAAAACCTT GTAGATTTCG
            781 CTGTATACAC CTTCTAATTT TGGAGCGTGA CATGTGGCCT CNATTGCATA AACTTTGTCG
            841 AAAGTGTTTT CTTCGAAATC CATTTTCNTG AAATCACCCT TTACAAAGTC CATTTGGTCA
            901 CTCNAATTGT ATTTTTTNAG CGNANTATTT TGCCTNGGCA ATTTNGNNNT CGNNATNGNT
            961 TANANNGATG ANNNNNCNAN NGGNAAATCT NGNNNNCNNN NNNGNNNGGC CCCNNACNCC
          1021 NCNNCNNNCN NNNANNNNNN ANNNNNNNNN N
          //
          Primer: CYC1ter_F
          Region: CYC1 ter, natNT2 (ADH1 ter, NrsR)
          Sequence file: RDB18657_B0Lne.seq check
          >D07536A3_B0Ln_2_CYC1ter_F_D04_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTTTNAT TTGCGGCCGA NCTCGATTAC
             61 AACAGGTGTT GTCCTCTGAG GACATAAAAT ACACACCGAG ATTCATCAAC TCATTGCTGG
            121 AGTTAGCATA TCTACAATTG GGTGAAATGG GGAGCGATTT GCAGGCATTT GCTCGGCATG
            181 CCGGTAGAGG TGTGGTCAAT AAGAGCGACC TCATGCTATA CCTGAGAAAG CAACCTGACC
            241 TACAGGAAAG AGTTACTCAA GAATAAGAAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA
            301 AATTTGTATA CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG
            361 AAATTCGCCT CGAGATTAGG GGCAGGGCAT GCTCATGTAG AGCGCCTGCT CGCCGTCCGA
            421 GGCGGTGCCG TCGTACAGGG CGGTGTCCAG GCCGCAGAGG GTGAACCCCA TCCGCCGGTA
            481 CGCGTGGATC GCCGGTGCGT TGACGTTGGT GACCTCCAGC CAGAGGTGCC CGGCGCCCCG
            541 CTCGCGGGCG AACTCCGTCG CGAGCCCCAT CAACGCGCGC CCGACCCCGT GCCCCCGGTG
            601 CTCCGGGGCG ACCTCGATGT CCTCGACGGT CAGCCGGCGG TTCCAGCCGG AGTACGAGAC
            661 GACCACGAAG CCCGCCAGGT CGCCGTCGTC CCCGTACGCG ACGAACGTCC GGGAGTCCGG
            721 GTCGCCGTCC TCCCCGTCGT CCGATTCGTC GTCCGATTCG TCGTCGGGGA ACACCTTGGT
            781 CAGGGGCGGG TCCACCGGCA CCTCCCGCAN GGTGAAGNCG TCCCCGGTGG CGGTGANGCN
            841 GAANANGGTG TCGGTGGTGN AGGANCCATN NNGTGCCTNN ATGGNCTCNG CGTCCCCCNG
            901 GANACTGGNG CNGNACCNGN AAGCCGTGNC NNCNAGANTG GTNNCCANNG TTGNTTNANG
            961 TTCNGNNGNN NNGTGAGANN TGTATNNNNN NNNGATTTTN AANNNNNNTA TATGAAAGAN
          1021 NAANNNNNNN NGNNNNAANN CNNNNNTNTN NNNNNNTTNN NNTNNNNGNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

          2021.11.17

          GNP_filter3_RDBDEP_html_211111.pl