Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYC136 pBS-GPDp-Erg6-V5-GFP11-CyC1ter-natNT2

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: lipid droplets.

Sequence (full)	RDB18657zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18657_B0Lnp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 Region: f1 ori, T7 pro, GPD pro Sequence file: RDB18657_B0Lna.seq
>D07536A3_B0Ln_2_f1ori_F2_F09_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTTCGNNCT NTTGNNNNNN NNAATGAGCT    61 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTCCATTCG   121 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC   181 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC   241 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC   301 GAATTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC   361 GTTTATCATT ATCAATACTC GCCATTTCAA AGAATACGTA AATAATTAAT AGTAGTGATT   421 TTCCTAACTT TATTTAGTCA AAAAATTAGC CTTTTAATTC TGCTGTAACC CGTACATGCC   481 CAAAATAGGG GGCGGGTTAC ACAGAATATA TAACATCGTA GGTGTCTGGG TGAACAGTTT   541 ATTCCTGGCA TCCACTAAAT ATAATGGAGC CCGCTTTTTA AGCTGGCATC CAGAAAAAAA   601 AAGAATCCCA GCACCAAAAT ATTGTTTTCT TCACCAACCA TCAGTTCATA GGTCCATTCT   661 CTTAGCGCAA CTACAGAGAA CAGGGGCACA AACAGGCAAA AAACGGGCAC AACCTCNNTG   721 GAGTGATGCA NCCTGCCTGG AGTAAATGAT GACACAAGGC AATTGACCCA CGCATGTATC   781 TATCTCATTT TCTTACACCT TCTATTACCT TCTGCTCTCT CTGANTTGNA AAAAGCTGAA   841 AAAAAAGGTT GAAACCNGTT CCCTGAAATT ATNCCCCTAC TTGANTAATA AGNATANNAA   901 AGACNGNAGG NATTGATTGN ANTNNGNAAA TCTNTTNCTT AAACNTCTTA AATTCNACTT 961 TTATAGNTAN NCTTTTTTTT ANNTTNNAAA NNNNNNNNNN //
Primer: pUC-CAPbs_up_F2 Region: T3 pro, natNT2 (TEF pro, NrsR) Sequence file: RDB18657_B0Lnb.seq
>D07536A3_B0Ln_2_pUC-CAPbs_up_F2_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNNTTTATG CTTCCGGCTC GTATGTTGTG    61 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA   121 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG   181 CTCTAGAACT AGTGGATCCC GGGTTAATTA AGGCGCGCCA GATCTGTTTA GCTTGCCTCG   241 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT   301 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC   361 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC   421 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT   481 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC   541 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT   601 AAACAACCAT GGGTACCACT CTTGACGACA CGGCTTACCG GTACCGCACC AGTGTCCCGG   661 GGGACGCCGA GGCCATCGAG GCACTGGATG GGTCCTTCAC CACCGACACC GTCTTCCGCG   721 TCACCGCCAC CGGGGANGGC TTCACCCTGC GGGAGGTGNC GGTGGACCCG CCCCTGANCA   781 AGGTGTTCCC CGACGACGAA TCGGANGANG AATCGGACGA NGGGGANGAN GGCGACNCGG   841 ANTNCCGGAN GTNCGTCGCG TACNGGGANG ANGGCNACCT GNCGGGCTTC GTGGNCGTCT   901 CGTACTCCGG CTGGNACCGN CGGCTGANCG TCNAGGANAT CGAGGTCGCC CCNNANCNNC   961 NGGGGGCNNG GGGNCNGGCG CGCNTTNNNG GGGNTCGCGA NNNANNNCGN CNGCNANNGG 1021 GGCNNNNNNN NNN //
Primer: TDH3pro_F Region: GPD pro, Erg6 5' Sequence file: RDB18657_B0Lnc.seq
>D07536A3_B0Ln_2_TDH3pro_F_E04_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNTGNNNTTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT    61 CTACTTTTAT AGTTAGTCTT TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT   121 GCGGCCGCAT GAGTGAAACA GAATTGAGAA AAAGACAGGC CCAATTCACT AGGGAGTTAC   181 ATGGTGATGA TATTGGTAAA AAGACAGGTT TGAGTGCATT GATGTCGAAG AACAACTCTG   241 CCCAAAAGGA AGCCGTTCAG AAGTACTTGA GAAATTGGGA TGGTAGAACC GATAAAGATG   301 CCGAAGAACG TCGTCTTGAG GATTATAATG AAGCCACACA TTCCTACTAT AACGTCGTTA   361 CAGATTTCTA TGAATATGGT TGGGGTTCCT CTTTCCATTT CAGCAGATTT TATAAAGGTG   421 AGAGTTTCGC TGCCTCGATA GCAAGACATG AACATTATTT AGCTTACAAG GCTGGTATTC   481 AAAGAGGCGA TTTAGTTCTC GACGTTGGTT GTGGTGTTGG GGGCCCAGCA AGAGAGATTG   541 CAAGATTTAC CGGTTGTAAC GTCATCGGTC TAAACAATAA CGATTACCAA ATTGCCAAGG   601 CAAAATATTA CGCTAAAAAA TACAATTTGA GTGACCAAAT GGACTTTGTA AAGGGTGATT   661 TCATGAAAAT GGATTTCGAA GAAAACACTT TCGACAAAGT TTATGCAATT GAGGCCACAT   721 GTCACGCTCC AAAATTAGAA GGTGTATACA GCGAAATCTA CAAGGTTTTG AAACCGGGTG   781 GTACCTTTGC TGTTTACNAA TGGGTAATGA CTGATAAATA TGACGAAAAC AATCCTGANC   841 ATAGAAAGAN CGCTTATGAA ATTGANNTAN GTGATGGNAT CCCNAAGATG TTCCNNGTCG   901 ACGNGGCTAG GAAAGCATTG AAGAACNGNG NNTTCNAANN CCTCNTNAGC GANNNNCTGN   961 NGNANNNGNT GANGAAANNC CNNNNNNNNN CCNNNTNNNN NGNNGANNNN NNGTANGTNN  1021 AAAACTNNNC TNANNTNNNN NNNNNTTTTN NNNNNNTNNN NNNNNNGGNN NNNNNNTTNN 1081 NNNNNNNN //
Primer: CYC1ter_R Region: CYC1 ter, GFP11, V5 tag, Erg6 3' Sequence file: RDB18657_B0Lnd.seq
>D07536A3_B0Ln_2_CYC1ter_R_C04_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NAGNGGNNGT GGGGGGAGGG CGTGAATGTA AGCGTGACAT    61 AACTAATTAC ATGACTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCTTAGGTA   121 ATGCCTGCAG CATTGACATA TTCGTGTAGA ACCATATGAT CTCTGCTTGT GGAACCACCA   181 CCACTTCCAC CAGTTGAGTC TAAGCCCAAA AGAGGGTTTG GTATGGGTTT CCCAGAACCT   241 CCGGCAGAGG ATCCTTGAGT TGCTTCTTGG GAAGTTTGGG AGGGGGTTTC GGCGTTTTCT   301 GGCTTCCTAG CGACGAAAAG CATCATTGGA GTGAATAACT TGGACTTACC ACCGGCAACT   361 AAACCAACCG CAGCATTTTC TAGAGCAGCA GTAACTTCCT TGGAACCTTC TGGGGCTAGA   421 CCTAATTTTT CCATTACAGT AACCATTGCT GTAGTAAATT GTCTACCCAA GTAAGAAGTT   481 CTGAAAAATG TGGCCAAATT AGCTAAGTTT TGAACGTACT TCCACTCACC AGTTAATGGG   541 TAATACCAAG GGATTTCATC ATCATTGTCC GCCAGGTCTT CGCTAACGAG GACTTCGAAA   601 CCACAGTTCT TCAATGCTTT CCTAGCCACG TCGACATGGA ACATCTTTGG GATACCATCA   661 CCTAGTTCAA TTTCATAAGC GATCTTTCTA TGTTCAGGAT TGTTTTCGTC ATATTTATCA   721 GTCATTACCC ATTCGTAAAC AGCAAAGGTA CCACCCGGTT TCAAAACCTT GTAGATTTCG   781 CTGTATACAC CTTCTAATTT TGGAGCGTGA CATGTGGCCT CNATTGCATA AACTTTGTCG   841 AAAGTGTTTT CTTCGAAATC CATTTTCNTG AAATCACCCT TTACAAAGTC CATTTGGTCA   901 CTCNAATTGT ATTTTTTNAG CGNANTATTT TGCCTNGGCA ATTTNGNNNT CGNNATNGNT   961 TANANNGATG ANNNNNCNAN NGGNAAATCT NGNNNNCNNN NNNGNNNGGC CCCNNACNCC 1021 NCNNCNNNCN NNNANNNNNN ANNNNNNNNN N //
Primer: CYC1ter_F Region: CYC1 ter, natNT2 (ADH1 ter, NrsR) Sequence file: RDB18657_B0Lne.seq
>D07536A3_B0Ln_2_CYC1ter_F_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNTTTNAT TTGCGGCCGA NCTCGATTAC    61 AACAGGTGTT GTCCTCTGAG GACATAAAAT ACACACCGAG ATTCATCAAC TCATTGCTGG   121 AGTTAGCATA TCTACAATTG GGTGAAATGG GGAGCGATTT GCAGGCATTT GCTCGGCATG   181 CCGGTAGAGG TGTGGTCAAT AAGAGCGACC TCATGCTATA CCTGAGAAAG CAACCTGACC   241 TACAGGAAAG AGTTACTCAA GAATAAGAAT TTTCGTTTTA AAACCTAAGA GTCACTTTAA   301 AATTTGTATA CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG   361 AAATTCGCCT CGAGATTAGG GGCAGGGCAT GCTCATGTAG AGCGCCTGCT CGCCGTCCGA   421 GGCGGTGCCG TCGTACAGGG CGGTGTCCAG GCCGCAGAGG GTGAACCCCA TCCGCCGGTA   481 CGCGTGGATC GCCGGTGCGT TGACGTTGGT GACCTCCAGC CAGAGGTGCC CGGCGCCCCG   541 CTCGCGGGCG AACTCCGTCG CGAGCCCCAT CAACGCGCGC CCGACCCCGT GCCCCCGGTG   601 CTCCGGGGCG ACCTCGATGT CCTCGACGGT CAGCCGGCGG TTCCAGCCGG AGTACGAGAC   661 GACCACGAAG CCCGCCAGGT CGCCGTCGTC CCCGTACGCG ACGAACGTCC GGGAGTCCGG   721 GTCGCCGTCC TCCCCGTCGT CCGATTCGTC GTCCGATTCG TCGTCGGGGA ACACCTTGGT   781 CAGGGGCGGG TCCACCGGCA CCTCCCGCAN GGTGAAGNCG TCCCCGGTGG CGGTGANGCN   841 GAANANGGTG TCGGTGGTGN AGGANCCATN NNGTGCCTNN ATGGNCTCNG CGTCCCCCNG   901 GANACTGGNG CNGNACCNGN AAGCCGTGNC NNCNAGANTG GTNNCCANNG TTGNTTNANG   961 TTCNGNNGNN NNGTGAGANN TGTATNNNNN NNNGATTTTN AANNNNNNTA TATGAAAGAN 1021 NAANNNNNNN NGNNNNAANN CNNNNNTNTN NNNNNNTTNN NNTNNNNGNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl