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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2alpha-PA

Expression vector of human eIF2Balpha with PA-tag at C-terminus.

Catalog number RDB18639
Resource name pEBMulti-Neo-human-eIF2alpha-PA
Clone info. Expression vector of human eukaryotic translation initiation factor 2B subunit alpha (eIF2Balpha) with PA-tag at C-terminus cloned into the pEBMulti-Neo.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Gene/insert name human EIF2B1 cDNA
Depositor|Developer Ito, Takuhiro |
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External Database
human EIF2B1

          Reference sequence
            
           
          Sequence (partial) RDB18639pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB18639 pEBMulti-Neo-human-eIF2alpha-PA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18639_B0L8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3
          Region: CAG pro, insert 5'
          Sequence file: RDB18639_B0L8a.seq check
          >D07570A1_B0L8_3w_pCAG-F_V3_E10_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCANGN CGGGGTTCGG CTTCTGGCGT
             61 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG
            121 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTAACCCTC
            181 ACTAAAGGGG TACCGGGCCC CCCCTCGAGG TCGACGCCAC CATGCCGGGT CTAAGTTGTA
            241 GATTTTATCA ACACAAATTT CCTGAGGTGG AAGATGTAGT GATGGTGAAT GTCAGATCCA
            301 TTGCTGAAAT GGGGGCTTAT GTCAGCTTGC TGGAATACAA CAACATTGAA GGCATGATTC
            361 TTCTTAGTGA ATTATCCAGA AGGCGTATCC GTTCTATCAA CAAACTCATC CGAATTGGCA
            421 GGAATGAGTG TGTGGTTGTC ATTAGGGTGG ACAAAGAAAA AGGATATATT GATTTGTCAA
            481 AAAGAAGAGT TTCTCCAGAG GAAGCAATCA AATGTGAAGA CAAATTCACA AAATCCAAAA
            541 CTGTTTATAG CATTCTTCGT CATGTTGCTG AGGTGTTAGA ATACACCAAG GATGAGCAGC
            601 TGGAAAGCCT ATTCCAGAGG ACTGCCTGGG TCTTTGATGA CAAGTACAAG AGACCTGGAT
            661 ATGGTGCCTA TGATGCATTT AAGCATGCAG TCTCAGACCC ATCTATTTTG GATAGTTTAG
            721 ATTTGAATGA AGATGAACGG GAAGTACTCA TTANTAATAT TAATAGGCGC TTGACCCCAC
            781 AGGCTGTCAA AATTCGAGCA NATATTGAAN TGGCTTGTTA TGGTTATGAA NGNATTGATG
            841 CTGTNAAANN ANCCCTAANA NCNNGNTTGA NTTGNNNNNN NGAAAACNTG NCCNTTANNA
            901 NTAATCTAAT AGCTCCTCCT CNGTANGNAA NGANTACNNN NACCCTGNAN ANAANANAAN
            961 NNCTTTCTGN CCTCNNNCAA NNTANNGNTG NNNNCNAANA NNANNNTGNN NAAANANGGN
          1021 NGNGNTCNAT GNTNNANTNG NNNCCNAAGN GNNCNNNNNN NNNN
          //
          Primer: pAxEF R2
          Region: SV40 polyA, insert 3'
          Sequence file: RDB18639_B0L8b.seq check
          >D07570A1_B0L8_3_pAxEF R2_B08_05_ABI24.ab1
              1 NNNNNNNNNN NNNNTTNNNT TGTNNANTTG TGNNGCTATT GCTTTATTTG TAACCATTAT
             61 AAGCTGCAAT AAACNANTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
            121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
            181 TGATCTAGAG TCGCGGCCGC TTACACCACA TCATCTTCGG CACCTGGCAT GGCAACGCCA
            241 TCTTCAGCTT TGGCTTCCAT TTCTTCTGCA TCATCATCTC CATCCACTTC GGCATTTTCT
            301 CTTTCAAGCC TCTCCATCTG CCTCGCAAGT TCAGTCTCAT CTGTATCTGT GACCACTTTG
            361 GGCTCCATTT GAACATTGAA CACACCCCTC TTTTCCTCAA TCTTCTCTTT GATAACAGCC
            421 ATAGCTTGAC TGAGGACAGA AAGGCCTTCT GTTCTCTCCA GGGTTGTCGT AGTCATTACA
            481 TACCGAGGAG GAGCTATTAG ATTAATCTTA ATGGGCATGT TTTCTGTAGA ACAATTCAAA
            541 CCTGCTCTTA GGGCTTCTTT TACAGCATCA ATGCCTTCAT AACCATAACA AGCCACTTCA
            601 ATATCTGCTC GAATTTTGAC AGCCTGTGGG GTCAAGCGCC TATTAATATT ATTAATGAGT
            661 ACTTCCCGTT CATCTTCATT CAAATCTAAA CTATCCAAAA TAGATGGGTC TGANACTGCA
            721 TGCTTAAATG CATCATAGGC ACCATATCCA GGTCTCTTGT ACTTGTCATC AAAGANCCAG
            781 GCAGTCCTCT GGAATANGCT TTCCAGCTGC TCATCCTTGG NGTATTCTAA CACCTCANCA
            841 NCNTGACGAA NAATGCTATA AACAGTTTTG NANTTTGNGA ATTTGNCTTC NCATTTGANT
            901 GCTNCNNNNG GANAAACNNT TCTTTTNGAN NANNCNATAN ATNCNTTTTT NNTNNNNNNN
          961 CNNNNNNNNN N
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori, NeoR_KanR
          Sequence file: RDB18639_B0L8c.seq check
          >D07570A1_B0L8_3_SV40pro_ori_F_C08_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCCTCN NGCCTCTGNN CTATTCNNNG AAGTANNTGA
             61 GGAGGCTTTT TTGGAGNNNC TNNNNNTTTT GCAAAGATCA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
            661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG NCGCTTTTCT GGATTCATCG ACTGNGGNCG GCTGGGTGTG GCGGANNGCT
            781 ATCANGACAT AGCGTTGGCT ACCCGTGATA TTGCTGANNA GCTTGNNGGC NAATGGNCTG
            841 ANCGCTTCCT CGTGCTTTNN NGNATCGCCN CTNCNGANNC NCAGCGCATC GNCNNTCTAT
            901 CGCCNTNNTT GACNAANTNN NTCNGANCGG GNNNNNNNGG GGNTNNNNAA ATGNNCNNNN
          961 CNNANNCGAN NNNNNNNNN
          //
          Primer: oriP_F
          Region: EBNA-1
          Sequence file: RDB18639_B0L8d.seq check
          >D07570A1_B0L8_3_oriP_F_D08_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGGNGNNN NTTTCNCTGC TTGTCCTTTT CCTGCTGGTT
             61 GCTCCCNTTC NTANGTGAAT TTAAGGAGGC CAGGCTAAAG CCGTCGCATG TCTGATTGCT
            121 CACCAGGTAA ATGTCGCTAA TGTTTTCCAA CGCGAGAAGG TGTTGAGCGC GGAGCTGAGT
            181 GACGTGACAA CATGGGTATG CCCAATTGCC CCATGTTGGG AGGACGAAAA TGGTGACAAG
            241 ACAGATGGCC AGAAATACAC CAACAGCACG CATGATGTCT ACTGGGGATT TATTCTTTAG
            301 TGCGGGGGAA TACACGGCTT TTAATACGAT TGAGGGCGTC TCCTAACAAG TTACATCACT
            361 CCTGCCCTTC CTCACCCTCA TCTCCATCAC CTCCTTCATC TCCGTCATCT CCGTCATCAC
            421 CCTCCGCGGC AGCCCCTTCC ACCATAGGTG GAAACCAGGG AGGCAAATCT ACTCCATCGT
            481 CAAAGCTGCA CACAGTCACC CTGATATTGC AGGTAGGAGC GGGCTTTGTC ATAACAAGGT
            541 CCTTAATCGC ATCCTTCAAA ACCTCAGCAA ATATATGAGT TTGTAAAAAG ACCATGAAAT
            601 AACAGACAAT GGACTCCCTT ANCGGGCCAG GTTGTGGGCC GGGTCCAGGG GCCATTCCAA
            661 AGGGGAGACG ACTCAATGGT GTAAGACGAC ATTGTGGAAT AGCAAGGGCA GTTCCTCGCC
            721 TTANGTTGTA AAGGGANGTC TTACTACCTC CATATACGAA CACACCGGNN ANCCAAGTTC
            781 CTTCGTCNGN AGNCCTTTCT ACGNGANTCC TANCNNNGNN AGCTCTNAAA CCTTCTGCNA
          841 NTGTNNTCAA ATTTNNNGNN NGGAACCNCN NNNANNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178.

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl