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pEBMulti-Neo-human-eIF2alpha-PA

Catalog number RDB18639
Resource name pEBMulti-Neo-human-eIF2alpha-PA
Vector backbone pEBMulti-Neo (Plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Depositor|Developer Ito, Takuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, AF. et al., Molecular Cell, S1097-2765(20)30738-3, 2020)
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RDB18639 pEBMulti-Neo-human-eIF2alpha-PA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB18639pka01.seq

Original reference

original Zyryanova, A.F., ISRIB Blunts the Integrated Stress Response by Allosterically Antagonising the Inhibitory Effect of Phosphorylated eIF2 on eIF2B. Mol. Cell S1097-2765 (20)30738-3 (2020). PMID 33220178.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18639_B0L8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F_V3
Region: CAG pro, insert 5'
Sequence file: RDB18639_B0L8a.seq check
>D07570A1_B0L8_3w_pCAG-F_V3_E10_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCANGN CGGGGTTCGG CTTCTGGCGT
   61 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG
  121 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTAACCCTC
  181 ACTAAAGGGG TACCGGGCCC CCCCTCGAGG TCGACGCCAC CATGCCGGGT CTAAGTTGTA
  241 GATTTTATCA ACACAAATTT CCTGAGGTGG AAGATGTAGT GATGGTGAAT GTCAGATCCA
  301 TTGCTGAAAT GGGGGCTTAT GTCAGCTTGC TGGAATACAA CAACATTGAA GGCATGATTC
  361 TTCTTAGTGA ATTATCCAGA AGGCGTATCC GTTCTATCAA CAAACTCATC CGAATTGGCA
  421 GGAATGAGTG TGTGGTTGTC ATTAGGGTGG ACAAAGAAAA AGGATATATT GATTTGTCAA
  481 AAAGAAGAGT TTCTCCAGAG GAAGCAATCA AATGTGAAGA CAAATTCACA AAATCCAAAA
  541 CTGTTTATAG CATTCTTCGT CATGTTGCTG AGGTGTTAGA ATACACCAAG GATGAGCAGC
  601 TGGAAAGCCT ATTCCAGAGG ACTGCCTGGG TCTTTGATGA CAAGTACAAG AGACCTGGAT
  661 ATGGTGCCTA TGATGCATTT AAGCATGCAG TCTCAGACCC ATCTATTTTG GATAGTTTAG
  721 ATTTGAATGA AGATGAACGG GAAGTACTCA TTANTAATAT TAATAGGCGC TTGACCCCAC
  781 AGGCTGTCAA AATTCGAGCA NATATTGAAN TGGCTTGTTA TGGTTATGAA NGNATTGATG
  841 CTGTNAAANN ANCCCTAANA NCNNGNTTGA NTTGNNNNNN NGAAAACNTG NCCNTTANNA
  901 NTAATCTAAT AGCTCCTCCT CNGTANGNAA NGANTACNNN NACCCTGNAN ANAANANAAN
  961 NNCTTTCTGN CCTCNNNCAA NNTANNGNTG NNNNCNAANA NNANNNTGNN NAAANANGGN
1021 NGNGNTCNAT GNTNNANTNG NNNCCNAAGN GNNCNNNNNN NNNN
//
Primer: pAxEF R2
Region: SV40 polyA, insert 3'
Sequence file: RDB18639_B0L8b.seq check
>D07570A1_B0L8_3_pAxEF R2_B08_05_ABI24.ab1
    1 NNNNNNNNNN NNNNTTNNNT TGTNNANTTG TGNNGCTATT GCTTTATTTG TAACCATTAT
   61 AAGCTGCAAT AAACNANTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
  121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
  181 TGATCTAGAG TCGCGGCCGC TTACACCACA TCATCTTCGG CACCTGGCAT GGCAACGCCA
  241 TCTTCAGCTT TGGCTTCCAT TTCTTCTGCA TCATCATCTC CATCCACTTC GGCATTTTCT
  301 CTTTCAAGCC TCTCCATCTG CCTCGCAAGT TCAGTCTCAT CTGTATCTGT GACCACTTTG
  361 GGCTCCATTT GAACATTGAA CACACCCCTC TTTTCCTCAA TCTTCTCTTT GATAACAGCC
  421 ATAGCTTGAC TGAGGACAGA AAGGCCTTCT GTTCTCTCCA GGGTTGTCGT AGTCATTACA
  481 TACCGAGGAG GAGCTATTAG ATTAATCTTA ATGGGCATGT TTTCTGTAGA ACAATTCAAA
  541 CCTGCTCTTA GGGCTTCTTT TACAGCATCA ATGCCTTCAT AACCATAACA AGCCACTTCA
  601 ATATCTGCTC GAATTTTGAC AGCCTGTGGG GTCAAGCGCC TATTAATATT ATTAATGAGT
  661 ACTTCCCGTT CATCTTCATT CAAATCTAAA CTATCCAAAA TAGATGGGTC TGANACTGCA
  721 TGCTTAAATG CATCATAGGC ACCATATCCA GGTCTCTTGT ACTTGTCATC AAAGANCCAG
  781 GCAGTCCTCT GGAATANGCT TTCCAGCTGC TCATCCTTGG NGTATTCTAA CACCTCANCA
  841 NCNTGACGAA NAATGCTATA AACAGTTTTG NANTTTGNGA ATTTGNCTTC NCATTTGANT
  901 GCTNCNNNNG GANAAACNNT TCTTTTNGAN NANNCNATAN ATNCNTTTTT NNTNNNNNNN
961 CNNNNNNNNN N
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB18639_B0L8c.seq check
>D07570A1_B0L8_3_SV40pro_ori_F_C08_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCCTCN NGCCTCTGNN CTATTCNNNG AAGTANNTGA
   61 GGAGGCTTTT TTGGAGNNNC TNNNNNTTTT GCAAAGATCA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG NCGCTTTTCT GGATTCATCG ACTGNGGNCG GCTGGGTGTG GCGGANNGCT
  781 ATCANGACAT AGCGTTGGCT ACCCGTGATA TTGCTGANNA GCTTGNNGGC NAATGGNCTG
  841 ANCGCTTCCT CGTGCTTTNN NGNATCGCCN CTNCNGANNC NCAGCGCATC GNCNNTCTAT
  901 CGCCNTNNTT GACNAANTNN NTCNGANCGG GNNNNNNNGG GGNTNNNNAA ATGNNCNNNN
961 CNNANNCGAN NNNNNNNNN
//
Primer: oriP_F
Region: EBNA-1
Sequence file: RDB18639_B0L8d.seq check
>D07570A1_B0L8_3_oriP_F_D08_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGNGNNN NTTTCNCTGC TTGTCCTTTT CCTGCTGGTT
   61 GCTCCCNTTC NTANGTGAAT TTAAGGAGGC CAGGCTAAAG CCGTCGCATG TCTGATTGCT
  121 CACCAGGTAA ATGTCGCTAA TGTTTTCCAA CGCGAGAAGG TGTTGAGCGC GGAGCTGAGT
  181 GACGTGACAA CATGGGTATG CCCAATTGCC CCATGTTGGG AGGACGAAAA TGGTGACAAG
  241 ACAGATGGCC AGAAATACAC CAACAGCACG CATGATGTCT ACTGGGGATT TATTCTTTAG
  301 TGCGGGGGAA TACACGGCTT TTAATACGAT TGAGGGCGTC TCCTAACAAG TTACATCACT
  361 CCTGCCCTTC CTCACCCTCA TCTCCATCAC CTCCTTCATC TCCGTCATCT CCGTCATCAC
  421 CCTCCGCGGC AGCCCCTTCC ACCATAGGTG GAAACCAGGG AGGCAAATCT ACTCCATCGT
  481 CAAAGCTGCA CACAGTCACC CTGATATTGC AGGTAGGAGC GGGCTTTGTC ATAACAAGGT
  541 CCTTAATCGC ATCCTTCAAA ACCTCAGCAA ATATATGAGT TTGTAAAAAG ACCATGAAAT
  601 AACAGACAAT GGACTCCCTT ANCGGGCCAG GTTGTGGGCC GGGTCCAGGG GCCATTCCAA
  661 AGGGGAGACG ACTCAATGGT GTAAGACGAC ATTGTGGAAT AGCAAGGGCA GTTCCTCGCC
  721 TTANGTTGTA AAGGGANGTC TTACTACCTC CATATACGAA CACACCGGNN ANCCAAGTTC
  781 CTTCGTCNGN AGNCCTTTCT ACGNGANTCC TANCNNNGNN AGCTCTNAAA CCTTCTGCNA
841 NTGTNNTCAA ATTTNNNGNN NGGAACCNCN NNNANNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Zyryanova, A.F., ISRIB Blunts the Integrated Stress Response by Allosterically Antagonising the Inhibitory Effect of Phosphorylated eIF2 on eIF2B. Mol. Cell S1097-2765 (20)30738-3 (2020). PMID 33220178.

2021.01.07

GNP_filter3_RDBDEP_html_201222.pl