Expression vector of human eIF2alpha with PA-tag at C-terminus.
Clone info. | Expression vector of human eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha) with PA-tag at C-terminus cloned into the pEBMulti-Neo. |
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Comment | Expression was confirmed by the depositor. |
Vector backbone | pEBMulti-Neo (plasmid) |
Size of vector backbone | 10.0 kb |
Selectable markers | Kanamycin (E. coli), Neomycin (mammalian cells) |
Growth conditions | LB+Kanamycin, 37oC |
Gene/insert name | human EIF2S1 cDNA |
Depositor|Developer | Ito, Takuhiro | |
Sequence (partial) | RDB18639pka01.seq provided by the depositor |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18639 | pEBMulti-Neo-human-eIF2alpha-PA | DNA solution |
Materials & Methods section:
The pEBMulti-Neo-human-eIF2alpha-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18639). |
Reference section:
Zyryanova, A.F., Kashiwagi, K., Rato, C., Harding, H.P., Crespillo-Casado, A., Perera, L.A., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18639_B0L8p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pCAG-F_V3 (Pr0495) Region: CAG pro, insert 5' Sequence file: RDB18639_B0L8a.seq ![]() |
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>D07570A1_B0L8_3w_pCAG-F_V3_E10_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGCANGN CGGGGTTCGG CTTCTGGCGT 61 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 121 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTAACCCTC 181 ACTAAAGGGG TACCGGGCCC CCCCTCGAGG TCGACGCCAC CATGCCGGGT CTAAGTTGTA 241 GATTTTATCA ACACAAATTT CCTGAGGTGG AAGATGTAGT GATGGTGAAT GTCAGATCCA 301 TTGCTGAAAT GGGGGCTTAT GTCAGCTTGC TGGAATACAA CAACATTGAA GGCATGATTC 361 TTCTTAGTGA ATTATCCAGA AGGCGTATCC GTTCTATCAA CAAACTCATC CGAATTGGCA 421 GGAATGAGTG TGTGGTTGTC ATTAGGGTGG ACAAAGAAAA AGGATATATT GATTTGTCAA 481 AAAGAAGAGT TTCTCCAGAG GAAGCAATCA AATGTGAAGA CAAATTCACA AAATCCAAAA 541 CTGTTTATAG CATTCTTCGT CATGTTGCTG AGGTGTTAGA ATACACCAAG GATGAGCAGC 601 TGGAAAGCCT ATTCCAGAGG ACTGCCTGGG TCTTTGATGA CAAGTACAAG AGACCTGGAT 661 ATGGTGCCTA TGATGCATTT AAGCATGCAG TCTCAGACCC ATCTATTTTG GATAGTTTAG 721 ATTTGAATGA AGATGAACGG GAAGTACTCA TTANTAATAT TAATAGGCGC TTGACCCCAC 781 AGGCTGTCAA AATTCGAGCA NATATTGAAN TGGCTTGTTA TGGTTATGAA NGNATTGATG 841 CTGTNAAANN ANCCCTAANA NCNNGNTTGA NTTGNNNNNN NGAAAACNTG NCCNTTANNA 901 NTAATCTAAT AGCTCCTCCT CNGTANGNAA NGANTACNNN NACCCTGNAN ANAANANAAN 961 NNCTTTCTGN CCTCNNNCAA NNTANNGNTG NNNNCNAANA NNANNNTGNN NAAANANGGN 1021 NGNGNTCNAT GNTNNANTNG NNNCCNAAGN GNNCNNNNNN NNNN // |
Primer: pAxEF R2 (Pr0059) Region: SV40 polyA, insert 3' Sequence file: RDB18639_B0L8b.seq ![]() |
>D07570A1_B0L8_3_pAxEF R2_B08_05_ABI24.ab1 1 NNNNNNNNNN NNNNTTNNNT TGTNNANTTG TGNNGCTATT GCTTTATTTG TAACCATTAT 61 AAGCTGCAAT AAACNANTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG 121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA 181 TGATCTAGAG TCGCGGCCGC TTACACCACA TCATCTTCGG CACCTGGCAT GGCAACGCCA 241 TCTTCAGCTT TGGCTTCCAT TTCTTCTGCA TCATCATCTC CATCCACTTC GGCATTTTCT 301 CTTTCAAGCC TCTCCATCTG CCTCGCAAGT TCAGTCTCAT CTGTATCTGT GACCACTTTG 361 GGCTCCATTT GAACATTGAA CACACCCCTC TTTTCCTCAA TCTTCTCTTT GATAACAGCC 421 ATAGCTTGAC TGAGGACAGA AAGGCCTTCT GTTCTCTCCA GGGTTGTCGT AGTCATTACA 481 TACCGAGGAG GAGCTATTAG ATTAATCTTA ATGGGCATGT TTTCTGTAGA ACAATTCAAA 541 CCTGCTCTTA GGGCTTCTTT TACAGCATCA ATGCCTTCAT AACCATAACA AGCCACTTCA 601 ATATCTGCTC GAATTTTGAC AGCCTGTGGG GTCAAGCGCC TATTAATATT ATTAATGAGT 661 ACTTCCCGTT CATCTTCATT CAAATCTAAA CTATCCAAAA TAGATGGGTC TGANACTGCA 721 TGCTTAAATG CATCATAGGC ACCATATCCA GGTCTCTTGT ACTTGTCATC AAAGANCCAG 781 GCAGTCCTCT GGAATANGCT TTCCAGCTGC TCATCCTTGG NGTATTCTAA CACCTCANCA 841 NCNTGACGAA NAATGCTATA AACAGTTTTG NANTTTGNGA ATTTGNCTTC NCATTTGANT 901 GCTNCNNNNG GANAAACNNT TCTTTTNGAN NANNCNATAN ATNCNTTTTT NNTNNNNNNN 961 CNNNNNNNNN N // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB18639_B0L8c.seq ![]() |
>D07570A1_B0L8_3_SV40pro_ori_F_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNCCTCN NGCCTCTGNN CTATTCNNNG AAGTANNTGA 61 GGAGGCTTTT TTGGAGNNNC TNNNNNTTTT GCAAAGATCA TCAAGAGACA GGATGAGGAT 121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 301 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA 661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 721 TGGAAAATGG NCGCTTTTCT GGATTCATCG ACTGNGGNCG GCTGGGTGTG GCGGANNGCT 781 ATCANGACAT AGCGTTGGCT ACCCGTGATA TTGCTGANNA GCTTGNNGGC NAATGGNCTG 841 ANCGCTTCCT CGTGCTTTNN NGNATCGCCN CTNCNGANNC NCAGCGCATC GNCNNTCTAT 901 CGCCNTNNTT GACNAANTNN NTCNGANCGG GNNNNNNNGG GGNTNNNNAA ATGNNCNNNN 961 CNNANNCGAN NNNNNNNNN // |
Primer: oriP_F (Pr0765) Region: EBNA-1 Sequence file: RDB18639_B0L8d.seq ![]() |
>D07570A1_B0L8_3_oriP_F_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGGNGNNN NTTTCNCTGC TTGTCCTTTT CCTGCTGGTT 61 GCTCCCNTTC NTANGTGAAT TTAAGGAGGC CAGGCTAAAG CCGTCGCATG TCTGATTGCT 121 CACCAGGTAA ATGTCGCTAA TGTTTTCCAA CGCGAGAAGG TGTTGAGCGC GGAGCTGAGT 181 GACGTGACAA CATGGGTATG CCCAATTGCC CCATGTTGGG AGGACGAAAA TGGTGACAAG 241 ACAGATGGCC AGAAATACAC CAACAGCACG CATGATGTCT ACTGGGGATT TATTCTTTAG 301 TGCGGGGGAA TACACGGCTT TTAATACGAT TGAGGGCGTC TCCTAACAAG TTACATCACT 361 CCTGCCCTTC CTCACCCTCA TCTCCATCAC CTCCTTCATC TCCGTCATCT CCGTCATCAC 421 CCTCCGCGGC AGCCCCTTCC ACCATAGGTG GAAACCAGGG AGGCAAATCT ACTCCATCGT 481 CAAAGCTGCA CACAGTCACC CTGATATTGC AGGTAGGAGC GGGCTTTGTC ATAACAAGGT 541 CCTTAATCGC ATCCTTCAAA ACCTCAGCAA ATATATGAGT TTGTAAAAAG ACCATGAAAT 601 AACAGACAAT GGACTCCCTT ANCGGGCCAG GTTGTGGGCC GGGTCCAGGG GCCATTCCAA 661 AGGGGAGACG ACTCAATGGT GTAAGACGAC ATTGTGGAAT AGCAAGGGCA GTTCCTCGCC 721 TTANGTTGTA AAGGGANGTC TTACTACCTC CATATACGAA CACACCGGNN ANCCAAGTTC 781 CTTCGTCNGN AGNCCTTTCT ACGNGANTCC TANCNNNGNN AGCTCTNAAA CCTTCTGCNA 841 NTGTNNTCAA ATTTNNNGNN NGGAACCNCN NNNANNNNNN NNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [link to RRC of NBRP] |
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