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pEBMulti-Neo-human-eIF2alpha-PA (#RDB18639)

Expression vector of human eIF2alpha with PA-tag at C-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Zyryanova, A.F., Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha) with PA-tag at C-terminus cloned into the pEBMulti-Neo.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human EIF2S1 cDNA
Depositor|Developer Ito, Takuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Zyryanova, A.F. et al., Mol. Cell 81: 88-103, 2021)。

Catalog # Resource name Shipping form Fee
RDB18639 pEBMulti-Neo-human-eIF2alpha-PA DNA solution

How to cite this biological resource

Materials & Methods section:

The pEBMulti-Neo-human-eIF2alpha-PA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18639).

Reference section:

Zyryanova, A.F., Kashiwagi, K., Rato, C., Harding, H.P., Crespillo-Casado, A., Perera, L.A., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Zyryanova, A.F., ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B. Mol. Cell 81 (1): 88-103 (2021). PMID 33220178. [PubMed] [Article] [RRC of NBRP]