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PKINES-venusx2-SV40 NLS/pcDNA (#RDB18605)

Expression vector of an NLS from SV40 and NES from human PKI (PKI NES-2x Venus-SV40 NLS).

Clone info. Expression vector of an importin-alpha/beta-dependent classical nuclear localization signal (NLS) from SV40 and nuclear export signal (NES) from human cAMP-dependent protein kinase inhibitor alpha (PKI) tagged with 2x Venus (PKI NES-2x Venus-SV40 NLS).
Comment Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name Human PKIA cDNA
Depositor|Developer Imaoka, Susumu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18605 PKINES-venusx2-SV40 NLS/pcDNA Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The PKINES-venusx2-SV40 NLS/pcDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18605).

Reference section:

Oguro, A., Imaoka, S., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Oguro, A., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]