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TMX2 WT/Flag pcDNA (#RDB18588)

Expression vector of human TMX2.

Clone info. Expression vector of human thioredoxin related transmembrane protein 2 (TMX2) tagged with 3x FLAG.
Comment This plasmid contains TMX2 corresponding isoform 1 (NP_057043.1).
Vector backbone 3xFlag-pcDNA4 (plasmid)
Gene/insert name Human TMX2 cDNA
Depositor|Developer Imaoka, Susumu |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguro, A. and Imaoka, S. Sci. Rep. 9 (1), 15296, 2019)。

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RDB18588 TMX2 WT/Flag pcDNA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The TMX2 WT/Flag pcDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18588).

Reference section:

Oguro, A., Imaoka, S., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18588_B0J9p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,2Xtet O,3XFLAG,insert 5'
Sequence file: RDB18588_B0J9a.seq check
>D07273A8_B0J9_3_CMV_Forward_A03_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN NGNGNNNNNN
   61 NNNNNNNNNC AGAGCTCTCC CTATCAGTGA TAGAGATCTC CCTATCAGTG ATAGAGATCG
  121 TCGACGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
  181 CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGGTTT AAACCATGGA CTACAAAGAC
  241 CATGACGGTG ATTATAAAGA TCATGACATC GATTACAAGG ATGACGATGA CAAGGGATCC
  301 GAAAAGATGG CGGTCTTGGC ACCTCTAATT GCTCTCGTGT ATTCGGTGCC GCGACTTTCA
  361 CGATGGCTCG CCCAACCTTA CTACCTTCTG TCGGCCCTGC TCTCTGCTGC CTTCCTACTC
  421 GTGAGGAAAC TGCCGCCGCT CTGCCACGGT CTGCCCACCC AACGCGAAGA CGGTAACCCG
  481 TGTGACTTTG ACTGGAGAGA AGTGGAGATC CTGATGTTTC TCAGTGCCAT TGTGATGATG
  541 AAGAACCGCA GATCCATCAC TGTGGAGCAA CATATAGGCA ACATTTTCAT GTTTAGTAAA
  601 GTGGCCAACA CAATTCTTTT CTTCCGCTTG GATATTCGCA TGGGCCTACT TTACATCACA
  661 CTCTGCATAG TGTTCCTGAT GACGTGCAAA CCCCCCCTAT ATATGGGCCC TGAGTATATC
  721 AAGTACTTCN NNGANNNNNN NCATTGATGA GGAACTAGAA CGGGACAAGA GGGTCACTTG
  781 GATTGTGGAG TTCTTTGCCN ATTGGTCTAA TGACTGCCAA TCATTTGCCC CTATCTATGC
  841 TGANCTCTCC NTTAAATACA ACTGTACNGG GCTAANNTTT GGNAANGTGG ANGNNNNNNN
  901 NTATACTGAT GTTAGTACGC GGTANAAAGT GANCACNTCA CCCCTCNNNA NNNNACTCCC
  961 TANCCNNGAT NNTNNNNCAA GGNGGCAAGG ANNNNNNGNN NCNGNCACAN ATNGANNAGA
1021 ANNNANGGGC TGNNNNNNNN NN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,insert 3'
Sequence file: RDB18588_B0J9b.seq check
>D07273A8_B0J9_3_bGH_rev3_B03_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGGGNNGG GCAAACNACN GANGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC GGGCCCTCTA GACTCGAGCG GCCGCCACTG
  121 TGCTGGATAT CTGCAGAATT CTTATTTATC CTTCTTGTTT TCCCCATCTG ACACTGTGGT
  181 GGGGGTTGAA GCCACAGGCT GCTCCTCAGG GATATTGTCT CCAGCCTTTG ATAGTTTCTT
  241 GGCCCGCTGG TATAGCTCAT TTAAGTTAAA TTCTCGGATC ACATTCTCCT CAGAGAAGGT
  301 CCATGAGACA GCCCGTCCTT TCTTGTCAAT CTGTGGCCGC CGCATTGCCT CCTTGCCACC
  361 TTGGAACAGG ATCAGGGTAG GGAGTTGCTT GGTGAGGGGT GATGTGCTCA CTTTGTACCG
  421 CGTACTAACA TCAGTATAGC GTCCAACATC CACCTTCCCA AAATTTAGCC CTGTACAGTT
  481 GTATTTAAGG GAGAGGTCAG CATAGATAGG GGCAAATGAT TGGCAGTCAT TAGACCAATT
  541 GGCAAAGAAC TCCACAATCC AAGTGACCCT CTTGTCCCGT TCTAGTTCCT CATCAATGGT
  601 TTTATCATTG AAGTACTTGA TATACTCAGG GCCCATATAT AGGGGGGGTT TGCACGTCAT
  661 CAGGAACACT ATGCAGAGTG TGATGTAAAG TAGGCCCATG CGAATATCCA AGCGGAAGAA
  721 AAGAATTGTG TTGGCCACTT TACTAAACAT GAAAATGTTG CCTATATGTT GCTCCACAGT
  781 GATGGATCTG CGGTTCTTCA TCATCACAAT GGCACTGAGA AACATCANGA TCTCCACTTC
  841 TCTCCAGTCA AAGTCACACN GGTTACCGTC TTCGCGTTGG GTGGGCAGAN CGTGGCANAN
  901 CGGCGGCNGT TTCCTCACNA NTANGAAGGN AGCANANANC NNGNCCNANA GAANGNNNNA
  961 ANGGNNGGGN NANCCATCNT GAAAGTCGCG NCANCNAATA CNCNANANCN ATTANAGGNG
 1021 NNNNNANNGN CNTCTTTTNN NANNCCNNNN NATCGTCNNN CNNNNANNCN NNGTCNNNNN
1081 NNNNN
//
Primer: f1oriF3 (Pr0437)
Region: f1 ori,2XSV40pro_ori
Sequence file: RDB18588_B0J9c.seq check
>D07273A8_B0J9_3_f1oriF3_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT NNNCCCTTNG ANGTTGGANT CCACGTTCTT
   61 TAATAGTGGA CTCTTGTTCC NAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT
  121 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA
  181 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA
  241 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT
  301 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA
  361 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC
  421 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT
  481 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
  541 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGACCT GTGGAATGTG
  601 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG
  661 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
  721 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC
  781 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGANT AATTTTTTTT
  841 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGANNTA GTGNNGAGGN
  901 TTTTTTGGAG GCCTANGCTT TTGNAAAAAN NTTANCATGA CCNANTANAA GCCCNCNGGN
  961 GCGCCTCGCC NNCCGCNANN ANNTCCCCAG GGNNNNNNGC ANCCTCGCCG CCNCGTTCGC
 1021 NNNNTANCCC GCNACGCGCN NNANCGNNNN ATNCNNANNG NNNNTNNAGN NGGGNNACNN
1081 NNNNNNNCAA NAANNNNTNN NNNNNNNN
//
Primer: reverse-2 (Pr0100)
Region: SV40 pA,PuroR
Sequence file: RDB18588_B0J9d.seq check
>D07273A8_B0J9_3_reverse-2_C05_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC CNNGCTCTAG CNNGAGGTCG ACGGTATACN GACATGATAA
   61 GATACATTGA TGAGTTNNGG ACNAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT
  121 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT
  181 GGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC
  241 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG
  301 TAGCACGACG ATGCATGGGG TCGTGCGCTC CTTTCGGTCG GGCGCTGCGG GTCGTGGGGC
  361 GGGCGTCAGG CACCGGGCTT GCGGGTCATG CACCAGGTGC GCGGTCCTTC GGGCACCTCG
  421 ACGTCGGCGG TGACGGTGAA GCCGAGCCGC TCGTAGAAGG GGAGGTTGCG GGGCGCGGAG
  481 GTCTCCAGGA AGGCGGGCAC CCCGGCGCGC TCGGCCGCCT CCACTCCGGG GAGCACGACG
  541 GCGCTGCCCA GACCCTTGCC CTGGTGGTCG GGCGAGACGC CGACGGTGGC CAGGAACCAC
  601 GCGGGCTCCT TGGGCCGGTG CGGCGCCAGG AGGCCTTCCA TCTGTTGCTG CGCGGCCAGC
  661 CGGGAACCGC TCAACTCGGC CATGCGCGGG CCGATCTCGG CGAACACCGC CCCCGCTTCG
  721 ACGCTCTCCG GCGTGGTCCA GACCGCCACC GCGGCGCCGT CGTCCGCGAN CCACACCTTG
  781 CCGATGTCGA GCCCGACGCG CGTGANGAAN AGTTCTTGCA GCTCGGTGAN CCGCTCNATG
  841 TGGCGGTCCG GATCGANGGT GTGGCGCGNG NCGGGGTAGT CGGCNAACNC GGCGGCGANG
  901 GNGCGTACNG NCCTGGGGAN GTCNNCNCGG GTGGNGAGGN GCACCGNGGG CTTGTACTCN
  961 GNCNNGGNAA NCNTTTTGCA AAANCCTNNN NNTNNNAAAA NCCTCNNCAC TACTTCTGGA
 1021 NANNTCANAN GNCNANGNNG CNNNNNCCNN NGNNNNANNN AAAANNNTNN TCNNNCNNNG
 1081 GNNNNNNNAN NGGGNNNNNN NGNNNNANTN NGGGNCNGNN NNNGGNNGNN NNNNNGGNNN
1141 NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Oguro, A., Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-beta-dependent nuclear cargo transport Sci. Rep. 9 (1): 15296 (2019). PMID 31653923. [link to RRC of NBRP]