The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: endoplasmic reticulum.
Alternative name | pSFL76, pRS314-GPDp-Ifa38-V5-GFP11 |
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Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS314 (plasmid) |
Selectable markers | Ampicillin (E. coli), TRP1 (S. cerevisiae). |
Gene/insert name | S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Tamura, Yasushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18577 | pSFL76 pRS314-GPDp-Ifa38-V5-GFP11 | DNA solution |
Materials & Methods section:
The pSFL76 pRS314-GPDp-Ifa38-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18577). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18577_B0J5p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro, Ifa38 5') Sequence file: RDB18577_B0J5a.seq ![]() |
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>D07393B2_B0J5_2_HB-NOS_R_A06_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNN NANNNNNNNG TAANGCCNGG GTTTTCCNNG TCNCGACGTT 61 GTAAAACGAC GGCCAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG GAGCTCAGTT 121 TATCATTATC AATACTCGCC ATTTCAAAGA ATACGTAAAT AATTAATAGT AGTGATTTTC 181 CTAACTTTAT TTAGTCAAAA AATTAGCCTT TTAATTCTGC TGTAACCCGT ACATGCCCAA 241 AATAGGGGGC GGGTTACACA GAATATATAA CATCGTAGGT GTCTGGGTGA ACAGTTTATT 301 CCTGGCATCC ACTAAATATA ATGGAGCCCG CTTTTTAAGC TGGCATCCAG AAAAAAAAAG 361 AATCCCAGCA CCAAAATATT GTTTTCTTCA CCAACCATCA GTTCATAGGT CCATTCTCTT 421 AGCGCAACTA CAGAGAACAG GGGCACAAAC AGGCAAAAAA CGGGCACAAC CTCAATGGAG 481 TGATGCAACC TGCCTGGAGT AAATGATGAC ACAAGGCAAT TGACCCACGC ATGTATCTAT 541 CTCATTTTCT TACACCTTCT ATTACCTTCT GCTCTCTCTG ATTTGGAAAA AGCTGAAAAA 601 AAAGGTTGAA ACCAGTTCCC TGAAATTATT CCCCTACTTG ACTAATAAGT ATATAAAGAC 661 GGTAGGTATT GATTGTAATT CTGTAAATCT ATTTCTTAAA CTTCTTAAAT TCTACTTTTA 721 TAGTTAGTCT TTTTTTTAGT TTTAAAACAC CAGAACTTAG TTTCGACGGA TGCGGCCGCA 781 TGACTTTTAT GCNACAGCTT CNAGANGCTG GGGAAAGANT TANGTGTATC AATGGTCTTT 841 TATGGGTTGN TTTTGGGCTT GGNGTCTTAA AATGNANNAC GTTATCACTT ANATTTTTAG 901 CTCNTANTTT TNATCNTTTT TTTACNANNN GCANNCNNTT TCNNNNAGNA TGGNGCTAAN 961 NNGGNNAATA CTGNGNNATC NCNNGNNNNN NGNGNNNGNN NNNGNNNANN NANNTNNNNN 1021 NNNNANNNGN NNNNNNNNN // |
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3') Sequence file: RDB18577_B0J5b.seq ![]() |
>D07393B2_B0J5_2_SV40A3'elementReverse_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNATGNNNN NNCNNGCNAC GCGGCCTTTT TACGGTTCCT 61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT 481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA 541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA 601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT 661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG 721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA 781 NGTAATGCCT GCAGCATTGA CATATTCGTG TAGAACCATA TGATCTCTGC TTGTGGAACC 841 ACCACCACTT CCACCAGTTG ANTCTAAGCC CAAAAGANGG TTTGGTATGG NTTTCCCANA 901 ANNCTCCGGC AGANGATNCN TCCTTTTTNN ACCTGTCTTG CGGCTTTTTT TNANNNNTCN 961 AATTCTGANA GATTTATGGN ANGGNANTNA NTNAANNNNN NTNNNNNANT NTTANNNNAA 1021 ANNNCNNNNN NNNNN // |
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB18577_B0J5c.seq ![]() |
>D07393B2_B0J5_2_TRP1pro_F_C06_09_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNAGGCAGC TTGGANTANG TCTGTTATTA ATTTCNCAGG 61 TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC 121 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT 181 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG 241 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT 301 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA 361 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT 421 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC 481 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA 541 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA 601 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG 661 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC 721 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCGA TCTATGCGGT GTGAAATACC 781 GCACAGATGC GTAAGGAGAA AATACCGCAT CANGAAATTG TAAGCGTTAA TATTTTGTTA 841 AAATTCGCGT TNAATTTTTG TTAAATCAGC TCATTTTTTA ACCNATANGC CGAAATCGGN 901 AAAATCCNNT ATAAATCNAA AGAATAGANN NAGANAGGGN TGANTGNTGN TNCNGTTTNN 961 AACNAGANTC CNNTATTAAA GANNNNNNNN TNNNNNNTCA AANGNNGAAA AANNNNCTAT 1021 NNNNNNNNNN // |
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, V5-GFP11, Ifa38 3') Sequence file: RDB18577_B0J5d.seq ![]() |
>D07393B2_B0J5_2_CYC1Ter_R_D06_12_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN ANNGGNNGTG GGGGGANGGC GTGAATGTAA GCGTGACATA 61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA 121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC 181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC 241 CGGCAGAGGA TCCTTCCTTT TTAACCTGTC TTGCGGCTTT TTTTAAGGCT CTAATTCTGA 301 TAGATTTATG GAAGGAATAA TTAATGGAGT TAACAATCTT AGAGTAAACG CCAAAGGTCT 361 CTGTAATTAC AAATTGATAC ACTGCGTGCG CCCAGTAAGG GGTCATAGTA GCGTATCTTT 421 CTTGAGAGCC GCAGCGTCTG CCAACGCTTC TTAAAGTGGA TTTTACAAAC TGTTGTGGAT 481 TTGGGATCAT TAAAGATGAT CTTCTGATTT TAGACATCGA GCTAGTGACC AAATATGAAA 541 TGATTAATTC AACATCGATA GCATCTTTAG ATAATTCCCC AGCCAAAGAG TTAGACCAAC 601 CTTGTAAGAA TGATTTCGAA CCACTGTATG TAGCCAAAAG TGGGGTGGGA ATCAGACCAC 661 CAAATGAGCC CATGGTTAAG ATCAAACCAC GAGTACCCGA CTTCTTGTTT TCAGCTTTCA 721 CAGTTTCCAC AATCTTTGGT GCAATGATTT GTGTAATTAA TAATGTAGCA GTGTTATTGA 781 TAGTGATAAT ATTTCTAAGC TCCTTCTCTT CTGNTTCCNA AAATGGAACA NGAATGGAGT 841 GTGATTGANC AACATTATTG ACCNAAACGG TGANTGGNAA CTGCGCACAC NATTNCTTAA 901 NGGATTCATA GTTGGANTCT TTATNCNCCG CAANGTCNAT GGNTAGAANC NTTANNNCGA 961 CNTGATGTTG ATCTTCTANN TCTTTTTGNA ANNNNNNCNN NTTTANANNN NGTNNNCNNN 1021 NNNNNNN // |
Primer: TDH3pro_F (Pr0431) Region: insert mid (GPD pro, Ifa38 5') Sequence file: RDB18577_B0J5e.seq ![]() |
>D07393B2_B0J5_2_TDH3pro_F_F06_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NTGNAATTCT GTAANTCTAT TTCTTAAACT TCTTAAATTC 61 TACTTTTATA GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG 121 CGGCCGCATG ACTTTTATGC AACAGCTTCA AGAGGCTGGG GAAAGATTTA GGTGTATCAA 181 TGGTCTTTTA TGGGTTGTTT TTGGGCTTGG TGTCTTAAAA TGTACAACGT TATCACTTAG 241 ATTTTTAGCT CTTATTTTTG ATCTTTTTTT ACTACCAGCA GTCAATTTCG ACAAGTATGG 301 TGCTAAAACT GGTAAATACT GTGCTATCAC TGGTGCAAGT GACGGTATTG GTAAAGAATT 361 TGCTAGACAA ATGGCGAAAC GTGGCTTCAA CTTGGTATTG ATCTCGAGAA CGCAATCTAA 421 ATTGGAGGCT TTACAAAAAG AACTAGAAGA TCAACATCAT GTCGTTGTAA AGATTCTAGC 481 CATTGACATT GCGGAGGATA AAGAATCCAA CTATGAATCC ATTAAGGAAT TGTGTGCGCA 541 GTTACCAATC ACCGTTTTGG TCAATAATGT TGGTCAATCA CACTCCATTC CTGTTCCATT 601 TTTGGAAACA GAAGAGAAGG AGCTTAGAAA TATTATCACT ATCAATAACA CTGCTACATT 661 ATTAATTACA CAAATCATTG CACCAAAGAN TGTGGAAACT GTGAAAGCTG AAAACAAGAA 721 GTCGGGTACT CGTGGTTTGA TCTTAACCAT GGGCTCATTT GGTGGTCTGA TTCCCACCCC 781 ACTTTTGGCT ACATACAGTG GTTCGAAATC ATTCTTACAA GGTTGGTCTA ACTCTTTGGC 841 TGGGGAATTA TCTAAAGATG CTATCGATGT TGAATTAATC ATTTCATATT TGGTCANTAN 901 CTCGATGNCT ANNNCNNAAN ATCATCTTTA ATGATCCNNA ATNNNCAACA GTTTGTAAAA 961 NCCANNTTAA NAANCGNNGG NANANNCNGN NNNNNNCNNN NANNNNNNNN NN // |
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18577_B0J5f.seq ![]() |
>D07393B2_B0J5_2_Amp_R_E06_15_ABI24.ab1 1 NNNNNNNNNN NNNNNTNNNN NNTNATCNGG GTTATTGTCT CNTGAGCGGA TACATATTTG 61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT 181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC 241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC 301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA 361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT 421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG 481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC 541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA 601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA 661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG 721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG 781 CGGATGCCGG GAGCAGANAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG 841 GCTGGCTTAN CTATGCGGCA TCAGANCANA NTGNACTGAG AGTGNACCNT ANATCACGAC 901 ATTACTATAT ATATAATATA NGAAGCNTTT AATANAANAG CATCGTAATA TATGTGNACT 961 TTGCNNTTAT GANNNNNGAN GGNAGTANNG NAANANANNC NTTANNGAAA AANANCNNGN 1021 NNNNNTNACN NANNNNNNNN NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
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