Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSFL76 pRS314-GPDp-Ifa38-V5-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with V5-GFP11 at C-terminus.

Catalog number RDB18577
Resource name pSFL76 pRS314-GPDp-Ifa38-V5-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18577 pSFL76 pRS314-GPDp-Ifa38-V5-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18577_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
Sequence file: RDB18577_B0J5a.seq check
>D07393B2_B0J5_2_HB-NOS_R_A06_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGNN NANNNNNNNG TAANGCCNGG GTTTTCCNNG TCNCGACGTT
   61 GTAAAACGAC GGCCAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG GAGCTCAGTT
  121 TATCATTATC AATACTCGCC ATTTCAAAGA ATACGTAAAT AATTAATAGT AGTGATTTTC
  181 CTAACTTTAT TTAGTCAAAA AATTAGCCTT TTAATTCTGC TGTAACCCGT ACATGCCCAA
  241 AATAGGGGGC GGGTTACACA GAATATATAA CATCGTAGGT GTCTGGGTGA ACAGTTTATT
  301 CCTGGCATCC ACTAAATATA ATGGAGCCCG CTTTTTAAGC TGGCATCCAG AAAAAAAAAG
  361 AATCCCAGCA CCAAAATATT GTTTTCTTCA CCAACCATCA GTTCATAGGT CCATTCTCTT
  421 AGCGCAACTA CAGAGAACAG GGGCACAAAC AGGCAAAAAA CGGGCACAAC CTCAATGGAG
  481 TGATGCAACC TGCCTGGAGT AAATGATGAC ACAAGGCAAT TGACCCACGC ATGTATCTAT
  541 CTCATTTTCT TACACCTTCT ATTACCTTCT GCTCTCTCTG ATTTGGAAAA AGCTGAAAAA
  601 AAAGGTTGAA ACCAGTTCCC TGAAATTATT CCCCTACTTG ACTAATAAGT ATATAAAGAC
  661 GGTAGGTATT GATTGTAATT CTGTAAATCT ATTTCTTAAA CTTCTTAAAT TCTACTTTTA
  721 TAGTTAGTCT TTTTTTTAGT TTTAAAACAC CAGAACTTAG TTTCGACGGA TGCGGCCGCA
  781 TGACTTTTAT GCNACAGCTT CNAGANGCTG GGGAAAGANT TANGTGTATC AATGGTCTTT
  841 TATGGGTTGN TTTTGGGCTT GGNGTCTTAA AATGNANNAC GTTATCACTT ANATTTTTAG
  901 CTCNTANTTT TNATCNTTTT TTTACNANNN GCANNCNNTT TCNNNNAGNA TGGNGCTAAN
  961 NNGGNNAATA CTGNGNNATC NCNNGNNNNN NGNGNNNGNN NNNGNNNANN NANNTNNNNN
1021 NNNNANNNGN NNNNNNNNN
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3')
Sequence file: RDB18577_B0J5b.seq check
>D07393B2_B0J5_2_SV40A3'elementReverse_B06_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNATGNNNN NNCNNGCNAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
  181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
  241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
  301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
  361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
  421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT
  481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA
  541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA
  601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT
  661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG
  721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA
  781 NGTAATGCCT GCAGCATTGA CATATTCGTG TAGAACCATA TGATCTCTGC TTGTGGAACC
  841 ACCACCACTT CCACCAGTTG ANTCTAAGCC CAAAAGANGG TTTGGTATGG NTTTCCCANA
  901 ANNCTCCGGC AGANGATNCN TCCTTTTTNN ACCTGTCTTG CGGCTTTTTT TNANNNNTCN
  961 AATTCTGANA GATTTATGGN ANGGNANTNA NTNAANNNNN NTNNNNNANT NTTANNNNAA
1021 ANNNCNNNNN NNNNN
//
Primer: TRP1pro_F
Region: TRP1
Sequence file: RDB18577_B0J5c.seq check
>D07393B2_B0J5_2_TRP1pro_F_C06_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNAGGCAGC TTGGANTANG TCTGTTATTA ATTTCNCAGG
   61 TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC
  121 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT
  181 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG
  241 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT
  301 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA
  361 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT
  421 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC
  481 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA
  541 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA
  601 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG
  661 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC
  721 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCGA TCTATGCGGT GTGAAATACC
  781 GCACAGATGC GTAAGGAGAA AATACCGCAT CANGAAATTG TAAGCGTTAA TATTTTGTTA
  841 AAATTCGCGT TNAATTTTTG TTAAATCAGC TCATTTTTTA ACCNATANGC CGAAATCGGN
  901 AAAATCCNNT ATAAATCNAA AGAATAGANN NAGANAGGGN TGANTGNTGN TNCNGTTTNN
  961 AACNAGANTC CNNTATTAAA GANNNNNNNN TNNNNNNTCA AANGNNGAAA AANNNNCTAT
1021 NNNNNNNNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, V5-GFP11, Ifa38 3')
Sequence file: RDB18577_B0J5d.seq check
>D07393B2_B0J5_2_CYC1Ter_R_D06_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNGNNNN ANNGGNNGTG GGGGGANGGC GTGAATGTAA GCGTGACATA
   61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA
  121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC
  181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC
  241 CGGCAGAGGA TCCTTCCTTT TTAACCTGTC TTGCGGCTTT TTTTAAGGCT CTAATTCTGA
  301 TAGATTTATG GAAGGAATAA TTAATGGAGT TAACAATCTT AGAGTAAACG CCAAAGGTCT
  361 CTGTAATTAC AAATTGATAC ACTGCGTGCG CCCAGTAAGG GGTCATAGTA GCGTATCTTT
  421 CTTGAGAGCC GCAGCGTCTG CCAACGCTTC TTAAAGTGGA TTTTACAAAC TGTTGTGGAT
  481 TTGGGATCAT TAAAGATGAT CTTCTGATTT TAGACATCGA GCTAGTGACC AAATATGAAA
  541 TGATTAATTC AACATCGATA GCATCTTTAG ATAATTCCCC AGCCAAAGAG TTAGACCAAC
  601 CTTGTAAGAA TGATTTCGAA CCACTGTATG TAGCCAAAAG TGGGGTGGGA ATCAGACCAC
  661 CAAATGAGCC CATGGTTAAG ATCAAACCAC GAGTACCCGA CTTCTTGTTT TCAGCTTTCA
  721 CAGTTTCCAC AATCTTTGGT GCAATGATTT GTGTAATTAA TAATGTAGCA GTGTTATTGA
  781 TAGTGATAAT ATTTCTAAGC TCCTTCTCTT CTGNTTCCNA AAATGGAACA NGAATGGAGT
  841 GTGATTGANC AACATTATTG ACCNAAACGG TGANTGGNAA CTGCGCACAC NATTNCTTAA
  901 NGGATTCATA GTTGGANTCT TTATNCNCCG CAANGTCNAT GGNTAGAANC NTTANNNCGA
  961 CNTGATGTTG ATCTTCTANN TCTTTTTGNA ANNNNNNCNN NTTTANANNN NGTNNNCNNN
1021 NNNNNNN
//
Primer: TDH3pro_F
Region: insert mid (GPD pro, Ifa38 5')
Sequence file: RDB18577_B0J5e.seq check
>D07393B2_B0J5_2_TDH3pro_F_F06_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGN NTGNAATTCT GTAANTCTAT TTCTTAAACT TCTTAAATTC
   61 TACTTTTATA GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG
  121 CGGCCGCATG ACTTTTATGC AACAGCTTCA AGAGGCTGGG GAAAGATTTA GGTGTATCAA
  181 TGGTCTTTTA TGGGTTGTTT TTGGGCTTGG TGTCTTAAAA TGTACAACGT TATCACTTAG
  241 ATTTTTAGCT CTTATTTTTG ATCTTTTTTT ACTACCAGCA GTCAATTTCG ACAAGTATGG
  301 TGCTAAAACT GGTAAATACT GTGCTATCAC TGGTGCAAGT GACGGTATTG GTAAAGAATT
  361 TGCTAGACAA ATGGCGAAAC GTGGCTTCAA CTTGGTATTG ATCTCGAGAA CGCAATCTAA
  421 ATTGGAGGCT TTACAAAAAG AACTAGAAGA TCAACATCAT GTCGTTGTAA AGATTCTAGC
  481 CATTGACATT GCGGAGGATA AAGAATCCAA CTATGAATCC ATTAAGGAAT TGTGTGCGCA
  541 GTTACCAATC ACCGTTTTGG TCAATAATGT TGGTCAATCA CACTCCATTC CTGTTCCATT
  601 TTTGGAAACA GAAGAGAAGG AGCTTAGAAA TATTATCACT ATCAATAACA CTGCTACATT
  661 ATTAATTACA CAAATCATTG CACCAAAGAN TGTGGAAACT GTGAAAGCTG AAAACAAGAA
  721 GTCGGGTACT CGTGGTTTGA TCTTAACCAT GGGCTCATTT GGTGGTCTGA TTCCCACCCC
  781 ACTTTTGGCT ACATACAGTG GTTCGAAATC ATTCTTACAA GGTTGGTCTA ACTCTTTGGC
  841 TGGGGAATTA TCTAAAGATG CTATCGATGT TGAATTAATC ATTTCATATT TGGTCANTAN
  901 CTCGATGNCT ANNNCNNAAN ATCATCTTTA ATGATCCNNA ATNNNCAACA GTTTGTAAAA
961 NCCANNTTAA NAANCGNNGG NANANNCNGN NNNNNNCNNN NANNNNNNNN NN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB18577_B0J5f.seq check
>D07393B2_B0J5_2_Amp_R_E06_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNNNN NNTNATCNGG GTTATTGTCT CNTGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
  181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
  241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
  301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
  361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
  421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
  481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
  541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
  601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
  661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
  721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
  781 CGGATGCCGG GAGCAGANAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
  841 GCTGGCTTAN CTATGCGGCA TCAGANCANA NTGNACTGAG AGTGNACCNT ANATCACGAC
  901 ATTACTATAT ATATAATATA NGAAGCNTTT AATANAANAG CATCGTAATA TATGTGNACT
  961 TTGCNNTTAT GANNNNNGAN GGNAGTANNG NAANANANNC NTTANNGAAA AANANCNNGN
1021 NNNNNTNACN NANNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2021.01.21

GNP_filter3_RDBDEP_html_201222.pl