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pSFL76 pRS314-GPDp-Ifa38-V5-GFP11

Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with V5-GFP11 at C-terminus.

Catalog number RDB18577
Resource name pSFL76 pRS314-GPDp-Ifa38-V5-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae ketoreductase (Ifa38) fused with V5-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name S. cerevisiae IFA38 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
S. cerevisiae IFA38 Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18577 pSFL76 pRS314-GPDp-Ifa38-V5-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualizing multiple inter-organelle contact sites (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18577_B0J5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (GPD pro, Ifa38 5')
          Sequence file: RDB18577_B0J5a.seq check
          >D07393B2_B0J5_2_HB-NOS_R_A06_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN NANNNNNNNG TAANGCCNGG GTTTTCCNNG TCNCGACGTT
             61 GTAAAACGAC GGCCAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG GAGCTCAGTT
            121 TATCATTATC AATACTCGCC ATTTCAAAGA ATACGTAAAT AATTAATAGT AGTGATTTTC
            181 CTAACTTTAT TTAGTCAAAA AATTAGCCTT TTAATTCTGC TGTAACCCGT ACATGCCCAA
            241 AATAGGGGGC GGGTTACACA GAATATATAA CATCGTAGGT GTCTGGGTGA ACAGTTTATT
            301 CCTGGCATCC ACTAAATATA ATGGAGCCCG CTTTTTAAGC TGGCATCCAG AAAAAAAAAG
            361 AATCCCAGCA CCAAAATATT GTTTTCTTCA CCAACCATCA GTTCATAGGT CCATTCTCTT
            421 AGCGCAACTA CAGAGAACAG GGGCACAAAC AGGCAAAAAA CGGGCACAAC CTCAATGGAG
            481 TGATGCAACC TGCCTGGAGT AAATGATGAC ACAAGGCAAT TGACCCACGC ATGTATCTAT
            541 CTCATTTTCT TACACCTTCT ATTACCTTCT GCTCTCTCTG ATTTGGAAAA AGCTGAAAAA
            601 AAAGGTTGAA ACCAGTTCCC TGAAATTATT CCCCTACTTG ACTAATAAGT ATATAAAGAC
            661 GGTAGGTATT GATTGTAATT CTGTAAATCT ATTTCTTAAA CTTCTTAAAT TCTACTTTTA
            721 TAGTTAGTCT TTTTTTTAGT TTTAAAACAC CAGAACTTAG TTTCGACGGA TGCGGCCGCA
            781 TGACTTTTAT GCNACAGCTT CNAGANGCTG GGGAAAGANT TANGTGTATC AATGGTCTTT
            841 TATGGGTTGN TTTTGGGCTT GGNGTCTTAA AATGNANNAC GTTATCACTT ANATTTTTAG
            901 CTCNTANTTT TNATCNTTTT TTTACNANNN GCANNCNNTT TCNNNNAGNA TGGNGCTAAN
            961 NNGGNNAATA CTGNGNNATC NCNNGNNNNN NGNGNNNGNN NNNGNNNANN NANNTNNNNN
          1021 NNNNANNNGN NNNNNNNNN
          //
          Primer: SV40pA3'Element Reverse
          Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3')
          Sequence file: RDB18577_B0J5b.seq check
          >D07393B2_B0J5_2_SV40A3'elementReverse_B06_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNATGNNNN NNCNNGCNAC GCGGCCTTTT TACGGTTCCT
             61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
            121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
            181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
            241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
            301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
            361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
            421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT
            481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA
            541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA
            601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT
            661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG
            721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA
            781 NGTAATGCCT GCAGCATTGA CATATTCGTG TAGAACCATA TGATCTCTGC TTGTGGAACC
            841 ACCACCACTT CCACCAGTTG ANTCTAAGCC CAAAAGANGG TTTGGTATGG NTTTCCCANA
            901 ANNCTCCGGC AGANGATNCN TCCTTTTTNN ACCTGTCTTG CGGCTTTTTT TNANNNNTCN
            961 AATTCTGANA GATTTATGGN ANGGNANTNA NTNAANNNNN NTNNNNNANT NTTANNNNAA
          1021 ANNNCNNNNN NNNNN
          //
          Primer: TRP1pro_F
          Region: TRP1
          Sequence file: RDB18577_B0J5c.seq check
          >D07393B2_B0J5_2_TRP1pro_F_C06_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNAGGCAGC TTGGANTANG TCTGTTATTA ATTTCNCAGG
             61 TAGTTCTGGT CCATTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC
            121 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT
            181 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG
            241 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT
            301 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA
            361 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT
            421 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC
            481 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA
            541 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA
            601 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG
            661 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC
            721 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCGA TCTATGCGGT GTGAAATACC
            781 GCACAGATGC GTAAGGAGAA AATACCGCAT CANGAAATTG TAAGCGTTAA TATTTTGTTA
            841 AAATTCGCGT TNAATTTTTG TTAAATCAGC TCATTTTTTA ACCNATANGC CGAAATCGGN
            901 AAAATCCNNT ATAAATCNAA AGAATAGANN NAGANAGGGN TGANTGNTGN TNCNGTTTNN
            961 AACNAGANTC CNNTATTAAA GANNNNNNNN TNNNNNNTCA AANGNNGAAA AANNNNCTAT
          1021 NNNNNNNNNN
          //
          Primer: CYC1ter_R
          Region: insert mid (CYC1 ter, V5-GFP11, Ifa38 3')
          Sequence file: RDB18577_B0J5d.seq check
          >D07393B2_B0J5_2_CYC1Ter_R_D06_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNNN ANNGGNNGTG GGGGGANGGC GTGAATGTAA GCGTGACATA
             61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA
            121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC
            181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC
            241 CGGCAGAGGA TCCTTCCTTT TTAACCTGTC TTGCGGCTTT TTTTAAGGCT CTAATTCTGA
            301 TAGATTTATG GAAGGAATAA TTAATGGAGT TAACAATCTT AGAGTAAACG CCAAAGGTCT
            361 CTGTAATTAC AAATTGATAC ACTGCGTGCG CCCAGTAAGG GGTCATAGTA GCGTATCTTT
            421 CTTGAGAGCC GCAGCGTCTG CCAACGCTTC TTAAAGTGGA TTTTACAAAC TGTTGTGGAT
            481 TTGGGATCAT TAAAGATGAT CTTCTGATTT TAGACATCGA GCTAGTGACC AAATATGAAA
            541 TGATTAATTC AACATCGATA GCATCTTTAG ATAATTCCCC AGCCAAAGAG TTAGACCAAC
            601 CTTGTAAGAA TGATTTCGAA CCACTGTATG TAGCCAAAAG TGGGGTGGGA ATCAGACCAC
            661 CAAATGAGCC CATGGTTAAG ATCAAACCAC GAGTACCCGA CTTCTTGTTT TCAGCTTTCA
            721 CAGTTTCCAC AATCTTTGGT GCAATGATTT GTGTAATTAA TAATGTAGCA GTGTTATTGA
            781 TAGTGATAAT ATTTCTAAGC TCCTTCTCTT CTGNTTCCNA AAATGGAACA NGAATGGAGT
            841 GTGATTGANC AACATTATTG ACCNAAACGG TGANTGGNAA CTGCGCACAC NATTNCTTAA
            901 NGGATTCATA GTTGGANTCT TTATNCNCCG CAANGTCNAT GGNTAGAANC NTTANNNCGA
            961 CNTGATGTTG ATCTTCTANN TCTTTTTGNA ANNNNNNCNN NTTTANANNN NGTNNNCNNN
          1021 NNNNNNN
          //
          Primer: TDH3pro_F
          Region: insert mid (GPD pro, Ifa38 5')
          Sequence file: RDB18577_B0J5e.seq check
          >D07393B2_B0J5_2_TDH3pro_F_F06_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NTGNAATTCT GTAANTCTAT TTCTTAAACT TCTTAAATTC
             61 TACTTTTATA GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG
            121 CGGCCGCATG ACTTTTATGC AACAGCTTCA AGAGGCTGGG GAAAGATTTA GGTGTATCAA
            181 TGGTCTTTTA TGGGTTGTTT TTGGGCTTGG TGTCTTAAAA TGTACAACGT TATCACTTAG
            241 ATTTTTAGCT CTTATTTTTG ATCTTTTTTT ACTACCAGCA GTCAATTTCG ACAAGTATGG
            301 TGCTAAAACT GGTAAATACT GTGCTATCAC TGGTGCAAGT GACGGTATTG GTAAAGAATT
            361 TGCTAGACAA ATGGCGAAAC GTGGCTTCAA CTTGGTATTG ATCTCGAGAA CGCAATCTAA
            421 ATTGGAGGCT TTACAAAAAG AACTAGAAGA TCAACATCAT GTCGTTGTAA AGATTCTAGC
            481 CATTGACATT GCGGAGGATA AAGAATCCAA CTATGAATCC ATTAAGGAAT TGTGTGCGCA
            541 GTTACCAATC ACCGTTTTGG TCAATAATGT TGGTCAATCA CACTCCATTC CTGTTCCATT
            601 TTTGGAAACA GAAGAGAAGG AGCTTAGAAA TATTATCACT ATCAATAACA CTGCTACATT
            661 ATTAATTACA CAAATCATTG CACCAAAGAN TGTGGAAACT GTGAAAGCTG AAAACAAGAA
            721 GTCGGGTACT CGTGGTTTGA TCTTAACCAT GGGCTCATTT GGTGGTCTGA TTCCCACCCC
            781 ACTTTTGGCT ACATACAGTG GTTCGAAATC ATTCTTACAA GGTTGGTCTA ACTCTTTGGC
            841 TGGGGAATTA TCTAAAGATG CTATCGATGT TGAATTAATC ATTTCATATT TGGTCANTAN
            901 CTCGATGNCT ANNNCNNAAN ATCATCTTTA ATGATCCNNA ATNNNCAACA GTTTGTAAAA
          961 NCCANNTTAA NAANCGNNGG NANANNCNGN NNNNNNCNNN NANNNNNNNN NN
          //
          Primer: Amp_R
          Region: AmpR pro, CEN_ARS
          Sequence file: RDB18577_B0J5f.seq check
          >D07393B2_B0J5_2_Amp_R_E06_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNTNNNN NNTNATCNGG GTTATTGTCT CNTGAGCGGA TACATATTTG
             61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGGGTCCTT TTCATCACGT GCTATAAAAA TAATTATAAT TTAAATTTTT TAATATAAAT
            181 ATATAAATTA AAAATAGAAA GTAAAAAAAG AAATTAAAGA AAAAATAGTT TTTGTTTTCC
            241 GAAGATGTAA AAGACTCTAG GGGGATCGCC AACAAATACT ACCTTTTATC TTGCTCTTCC
            301 TGCTCTCAGG TATTAATGCC GAATTGTTTC ATCTTGTCTG TGTAGAAGAC CACACACGAA
            361 AATCCTGTGA TTTTACATTT TACTTATCGT TAATCGAATG TATATCTATT TAATCTGCTT
            421 TTCTTGTCTA ATAAATATAT ATGTAAAGTA CGCTTTTTGT TGAAATTTTT TAAACCTTTG
            481 TTTATTTTTT TTTCTTCATT CCGTAACTCT TCTACCTTCT TTATTTACTT TCTAAAATCC
            541 AAATACAAAA CATAAAAATA AATAAACACA GAGTAAATTC CCAAATTATT CCATCATTAA
            601 AAGATACGAG GCGCGTGTAA GTTACAGGCA AGCGATCCGT CTAAGAAACC ATTATTATCA
            661 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
            721 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
            781 CGGATGCCGG GAGCAGANAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
            841 GCTGGCTTAN CTATGCGGCA TCAGANCANA NTGNACTGAG AGTGNACCNT ANATCACGAC
            901 ATTACTATAT ATATAATATA NGAAGCNTTT AATANAANAG CATCGTAATA TATGTGNACT
            961 TTGCNNTTAT GANNNNNGAN GGNAGTANNG NAANANANNC NTTANNGAAA AANANCNNGN
          1021 NNNNNTNACN NANNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

          2022.05.16

          GNP_filter3_RDBDEP_html_220516.pl