Expression vector of V5-GFP11.
Clone info. | The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of V5-GFP11, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018. |
---|---|
Comment | Expression was confirmed by the depositor with fluorescence microscopy. |
Vector backbone | pRS314 (plasmid) |
Selectable markers | Ampicillin (E. coli), TRP1 (S. cerevisiae). |
Gene/insert name | Aequorea victoria GFP cDNA |
Depositor|Developer | Tamura, Yasushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
---|---|
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
---|---|
MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB18576 | pSFL74 pRS314-GPDp-V5-GFP11 | DNA solution |
Materials & Methods section:
The pSFL74 pRS314-GPDp-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18576). |
Reference section:
Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB18576_B0J5p1-1.pdf ![]() |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: HB-NOS_R (Pr0800) Region: T7 pro, insert 5' (GPD pro) Sequence file: RDB18576_B0J5a.seq ![]() |
---|
>D07393B1_B0J5_2_HB-NOS_R_A05_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNN NNNNGGGTNN NGCCNGGGTT TTCCCNGTCA CGACGTTGTA 61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT 121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA 181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT 241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT 301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT 361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC 421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA 481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC 541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA 601 GGTTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT 661 AGGTATTGAT TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG 721 TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCTCTA 781 GAACTAGTGG ATCCTCTGCC GGANGTTCTG GGAAACCCAT ACCAAANCCT CTTTTGGGCT 841 TAGANTCNAC TGGTGGAANN GGNGGNGGNT NCNCAAGCAG ANATCATATG GNTCTACNCN 901 AATATGNCNN NGCTGCANGN ATTANCTAAN AATTCNANAT CNAGCNTATC GATANNNNCN 961 ANNNNNANNC ANGNNNNTAN NTATGNNNNG CNNNNNTTCN NGCCCNNCCN CNNNNNNNNN 1021 N // |
Primer: SV40pA3'Element Reverse (Pr0108) Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3') Sequence file: RDB18576_B0J5b.seq ![]() |
>D07393B1_B0J5_2_SV40A3'elementReverse_B05_05_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA NGCCNGCAAC GCGGCCTTTT TACGGTTCCT 61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT 481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA 541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA 601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT 661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG 721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA 781 GGTAATGCCT GCAGCATTGA CATATTCGTG TAGAANCATA TGATCTCTGC TTGTGGAACC 841 ACCACCACTT CCACCAGTTG AGTCTAAGCC NNAAAGANGG TTNGGNATGG GTTNCCCAGA 901 ACCNCCNGCA GAGGANNNAC TANTNCNANN NCGGCCGCAT CCGNCGAANN TAANNTCTGG 961 NGNTTTAAAA CTNANAAAAA ANNNTNNNTA NNAAANNANA ANTTTNANNN NNNTNNNNNN 1021 NNNN // |
Primer: TRP1pro_F (Pr0171) Region: TRP1, f1 ori Sequence file: RDB18576_B0J5c.seq ![]() |
>D07393B1_B0J5_2_TRP1pro_F_C05_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCAGC TTGGAGTANG TCTGTTATTA ATTTCNCAGG 61 TAGTTCTGGT CCNTTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC 121 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT 181 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG 241 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT 301 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA 361 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT 421 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC 481 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA 541 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA 601 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG 661 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC 721 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCGA TCTATGCGGT GTGAAATACC 781 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGAAATTG TAAGCGTTAA TATTTTGTTN 841 AAATTCGCGT TNAATTTTTG TTAAATCANC TCATTTTTTA ANNNATAGGN CGAAATCNNN 901 AAAATCCCTT ATAAATCAAA AGAATAGANC GAGATAGGGT TGAGNGNTGN TCCNGTTTNN 961 NNNNNGANTC CNCTNNTAAN NAACGNGNAN NNNNACNTCN ANNGNCGAAA AANNGTCNAT 1021 CNNNNNANNN NCNNNNACNN NNNNNNNN // |
Primer: CYC1ter_R (Pr0022) Region: insert mid (CYC1 ter, V5-GFP11, GPD pro) Sequence file: RDB18576_B0J5d.seq ![]() |
>D07393B1_B0J5_2_CYC1Ter_R_D05_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN AGNGGNNGTG GGGGGANGGC GTGAATGTAA GCGTGACATA 61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA 121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC 181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC 241 CGGCAGAGGA TCCACTAGTT CTAGAGCGGC CGCATCCGTC GAAACTAAGT TCTGGTGTTT 301 TAAAACTAAA AAAAAGACTA ACTATAAAAG TAGAATTTAA GAAGTTTAAG AAATAGATTT 361 ACAGAATTAC AATCAATACC TACCGTCTTT ATATACTTAT TAGTCAAGTA GGGGAATAAT 421 TTCAGGGAAC TGGTTTCAAC CTTTTTTTTC AGCTTTTTCC AAATCAGAGA GAGCAGAAGG 481 TAATAGAAGG TGTAAGAAAA TGAGATAGAT ACATGCGTGG GTCAATTGCC TTGTGTCATC 541 ATTTACTCCA GGCAGGTTGC ATCACTCCAT TGAGGTTGTG CCCGTTTTTT GCCTGTTTGT 601 GCCCCTGTTC TCTGTAGTTG CGCTAAGAGA ATGGACCTAT GAACTGATGG TTGGTGAAGA 661 AAACAATATT TTGGTGCTGG GATTCTTTTT TTTTCTGGAT GCCAGCTTAA AAAGCGGGCT 721 CCATTATATT TAGTGGATGC CAGGAATAAA CTGTTCACCC AGACACCTAC GATGTTATAT 781 ATTCTGTGTA ACCCGCCCCC TATTTTGGGC ATGTACGGGT TACAGCAGAA TTAAAAGGCT 841 AATTTTTTGA CTAAATAAAG TTAGGAAAAT CACTACTATT NATTATTTAC GTATTCTTTG 901 AAATGNCGAG TANTGATAAT GATAAACTGA NCTCCNATTC GCCCTATAGN GAGNCGNATT 961 ACAATTCANN GGNCNNNCNT TTTNCNNCGT NNNNNNNGGG AAAACCNNNN NNNTNCCCNA 1021 NCNTNNNNNN NNNN // |
Primer: Amp_R (Pr0008) Region: AmpR pro, CEN_ARS Sequence file: RDB18576_B0J5e.seq ![]() |
>D07393B1_B0J5_2_Amp_R_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNTNNNT GNNNCANTTA TNNGGGTTAT TGTCTCNTGG ANNCGGATAC 61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 121 GTGCCACCTG GGTCCTTTTC ATCACGTGCT ATAAAAATAA TTATAATTTA AATTTTTTAA 181 TATAAATATA TAAATTAAAA ATAGAAAGTA AAAAAAGAAA TTAAAGAAAA AATAGTTTTT 241 GTTTTCCGAA GATGTAAAAG ACTCTAGGGG GATCGCCAAC AAATACTACC TTTTATCTTG 301 CTCTTCCTGC TCTCAGGTAT TAATGCCGAA TTGTTTCATC TTGTCTGTGT AGAAGACCAC 361 ACACGAAAAT CCTGTGATTT TACATTTTAC TTATCGTTAA TCGAATGTAT ATCTATTTAA 421 TCTGCTTTTC TTGTCTAATA AATATATATG TAAAGTACGC TTTTTGTTGA AATTTTTTAA 481 ACCTTTGTTT ATTTTTTTTT CTTCATTCCG TAACTCTTCT ACCTTCTTTA TTTACTTTCT 541 AAAATCCAAA TACAAAACAT AAAAATAAAT AAACACAGAG TAAATTCCCA AATTATTCCA 601 TCATTAAAAG ATACGAGGCG CGTGTAAGTT ACAGGCAAGC GATCCGTCTA AGAAACCATT 661 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT 721 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT 781 CTGTAAGCGG ANGCCGGGAG CAGANAAGCC CGTCAGGGCG CGTCAGCGGG NGTTGGNGGG 841 TGTCGGGGCT GGCTTAACTA TGCGGNATCN GANCANANTG NACTGANAGT GCNCCATANA 901 TCACGACNTT ACTATATATA TAATATAGGA AGCATTTAAT ANAACAGCAT CGNAATANAT 961 GTGNACNTTG CANTNNNANG CNNGANGGCA NTNNNNNNNA NNTNCTTTAN NGNAAAATAN 1021 NTTGNNNNNN NNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP] |
---|
Featured content