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pSFL74 pRS314-GPDp-V5-GFP11

Expression vector of V5-GFP11.

Catalog number RDB18576
Resource name pSFL74 pRS314-GPDp-V5-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of V5-GFP11, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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RDB18576 pSFL74 pRS314-GPDp-V5-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18576_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB18576_B0J5a.seq check
>D07393B1_B0J5_2_HB-NOS_R_A05_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNNNGGGTNN NGCCNGGGTT TTCCCNGTCA CGACGTTGTA
   61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT
  121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA
  181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT
  241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT
  301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT
  361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC
  421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA
  481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC
  541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA
  601 GGTTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGACT AATAAGTATA TAAAGACGGT
  661 AGGTATTGAT TGTAATTCTG TAAATCTATT TCTTAAACTT CTTAAATTCT ACTTTTATAG
  721 TTAGTCTTTT TTTTAGTTTT AAAACACCAG AACTTAGTTT CGACGGATGC GGCCGCTCTA
  781 GAACTAGTGG ATCCTCTGCC GGANGTTCTG GGAAACCCAT ACCAAANCCT CTTTTGGGCT
  841 TAGANTCNAC TGGTGGAANN GGNGGNGGNT NCNCAAGCAG ANATCATATG GNTCTACNCN
  901 AATATGNCNN NGCTGCANGN ATTANCTAAN AATTCNANAT CNAGCNTATC GATANNNNCN
  961 ANNNNNANNC ANGNNNNTAN NTATGNNNNG CNNNNNTTCN NGCCCNNCCN CNNNNNNNNN
1021 N
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, V5-GFP11 3')
Sequence file: RDB18576_B0J5b.seq check
>D07393B1_B0J5_2_SV40A3'elementReverse_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNA NGCCNGCAAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
  181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
  241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
  301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
  361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
  421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT
  481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA
  541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA
  601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT
  661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG
  721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA
  781 GGTAATGCCT GCAGCATTGA CATATTCGTG TAGAANCATA TGATCTCTGC TTGTGGAACC
  841 ACCACCACTT CCACCAGTTG AGTCTAAGCC NNAAAGANGG TTNGGNATGG GTTNCCCAGA
  901 ACCNCCNGCA GAGGANNNAC TANTNCNANN NCGGCCGCAT CCGNCGAANN TAANNTCTGG
  961 NGNTTTAAAA CTNANAAAAA ANNNTNNNTA NNAAANNANA ANTTTNANNN NNNTNNNNNN
1021 NNNN
//
Primer: TRP1pro_F
Region: TRP1, f1 ori
Sequence file: RDB18576_B0J5c.seq check
>D07393B1_B0J5_2_TRP1pro_F_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCAGC TTGGAGTANG TCTGTTATTA ATTTCNCAGG
   61 TAGTTCTGGT CCNTTGGTGA AAGTTTGCGG CTTGCAGAGC ACAGAGGCCG CAGAATGTGC
  121 TCTAGATTCC GATGCTGACT TGCTGGGTAT TATATGTGTG CCCAATAGAA AGAGAACAAT
  181 TGACCCGGTT ATTGCAAGGA AAATTTCAAG TCTTGTAAAA GCATATAAAA ATAGTTCAGG
  241 CACTCCGAAA TACTTGGTTG GCGTGTTTCG TAATCAACCT AAGGAGGATG TTTTGGCTCT
  301 GGTCAATGAT TACGGCATTG ATATCGTCCA ACTGCATGGA GATGAGTCGT GGCAAGAATA
  361 CCAAGAGTTC CTCGGTTTGC CAGTTATTAA AAGACTCGTA TTTCCAAAAG ACTGCAACAT
  421 ACTACTCAGT GCAGCTTCAC AGAAACCTCA TTCGTTTATT CCCTTGTTTG ATTCAGAAGC
  481 AGGTGGGACA GGTGAACTTT TGGATTGGAA CTCGATTTCT GACTGGGTTG GAAGGCAAGA
  541 GAGCCCCGAA AGCTTACATT TTATGTTAGC TGGTGGACTG ACGCCAGAAA ATGTTGGTGA
  601 TGCGCTTAGA TTAAATGGCG TTATTGGTGT TGATGTAAGC GGAGGTGTGG AGACAAATGG
  661 TGTAAAAGAC TCTAACAAAA TAGCAAATTT CGTCAAAAAT GCTAAGAAAT AGGTTATTAC
  721 TGAGTAGTAT TTATTTAAGT ATTGTTTGTG CACTTGCCGA TCTATGCGGT GTGAAATACC
  781 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGAAATTG TAAGCGTTAA TATTTTGTTN
  841 AAATTCGCGT TNAATTTTTG TTAAATCANC TCATTTTTTA ANNNATAGGN CGAAATCNNN
  901 AAAATCCCTT ATAAATCAAA AGAATAGANC GAGATAGGGT TGAGNGNTGN TCCNGTTTNN
  961 NNNNNGANTC CNCTNNTAAN NAACGNGNAN NNNNACNTCN ANNGNCGAAA AANNGTCNAT
1021 CNNNNNANNN NCNNNNACNN NNNNNNNN
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, V5-GFP11, GPD pro)
Sequence file: RDB18576_B0J5d.seq check
>D07393B1_B0J5_2_CYC1Ter_R_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN AGNGGNNGTG GGGGGANGGC GTGAATGTAA GCGTGACATA
   61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA
  121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC
  181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC
  241 CGGCAGAGGA TCCACTAGTT CTAGAGCGGC CGCATCCGTC GAAACTAAGT TCTGGTGTTT
  301 TAAAACTAAA AAAAAGACTA ACTATAAAAG TAGAATTTAA GAAGTTTAAG AAATAGATTT
  361 ACAGAATTAC AATCAATACC TACCGTCTTT ATATACTTAT TAGTCAAGTA GGGGAATAAT
  421 TTCAGGGAAC TGGTTTCAAC CTTTTTTTTC AGCTTTTTCC AAATCAGAGA GAGCAGAAGG
  481 TAATAGAAGG TGTAAGAAAA TGAGATAGAT ACATGCGTGG GTCAATTGCC TTGTGTCATC
  541 ATTTACTCCA GGCAGGTTGC ATCACTCCAT TGAGGTTGTG CCCGTTTTTT GCCTGTTTGT
  601 GCCCCTGTTC TCTGTAGTTG CGCTAAGAGA ATGGACCTAT GAACTGATGG TTGGTGAAGA
  661 AAACAATATT TTGGTGCTGG GATTCTTTTT TTTTCTGGAT GCCAGCTTAA AAAGCGGGCT
  721 CCATTATATT TAGTGGATGC CAGGAATAAA CTGTTCACCC AGACACCTAC GATGTTATAT
  781 ATTCTGTGTA ACCCGCCCCC TATTTTGGGC ATGTACGGGT TACAGCAGAA TTAAAAGGCT
  841 AATTTTTTGA CTAAATAAAG TTAGGAAAAT CACTACTATT NATTATTTAC GTATTCTTTG
  901 AAATGNCGAG TANTGATAAT GATAAACTGA NCTCCNATTC GCCCTATAGN GAGNCGNATT
  961 ACAATTCANN GGNCNNNCNT TTTNCNNCGT NNNNNNNGGG AAAACCNNNN NNNTNCCCNA
1021 NCNTNNNNNN NNNN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB18576_B0J5e.seq check
>D07393B1_B0J5_2_Amp_R_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNTNNNT GNNNCANTTA TNNGGGTTAT TGTCTCNTGG ANNCGGATAC
   61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG GGTCCTTTTC ATCACGTGCT ATAAAAATAA TTATAATTTA AATTTTTTAA
  181 TATAAATATA TAAATTAAAA ATAGAAAGTA AAAAAAGAAA TTAAAGAAAA AATAGTTTTT
  241 GTTTTCCGAA GATGTAAAAG ACTCTAGGGG GATCGCCAAC AAATACTACC TTTTATCTTG
  301 CTCTTCCTGC TCTCAGGTAT TAATGCCGAA TTGTTTCATC TTGTCTGTGT AGAAGACCAC
  361 ACACGAAAAT CCTGTGATTT TACATTTTAC TTATCGTTAA TCGAATGTAT ATCTATTTAA
  421 TCTGCTTTTC TTGTCTAATA AATATATATG TAAAGTACGC TTTTTGTTGA AATTTTTTAA
  481 ACCTTTGTTT ATTTTTTTTT CTTCATTCCG TAACTCTTCT ACCTTCTTTA TTTACTTTCT
  541 AAAATCCAAA TACAAAACAT AAAAATAAAT AAACACAGAG TAAATTCCCA AATTATTCCA
  601 TCATTAAAAG ATACGAGGCG CGTGTAAGTT ACAGGCAAGC GATCCGTCTA AGAAACCATT
  661 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT
  721 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT
  781 CTGTAAGCGG ANGCCGGGAG CAGANAAGCC CGTCAGGGCG CGTCAGCGGG NGTTGGNGGG
  841 TGTCGGGGCT GGCTTAACTA TGCGGNATCN GANCANANTG NACTGANAGT GCNCCATANA
  901 TCACGACNTT ACTATATATA TAATATAGGA AGCATTTAAT ANAACAGCAT CGNAATANAT
  961 GTGNACNTTG CANTNNNANG CNNGANGGCA NTNNNNNNNA NNTNCTTTAN NGNAAAATAN
1021 NTTGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2021.01.21

GNP_filter3_RDBDEP_html_201222.pl