RDB18575 - pSFL73 pRS314-GPDp-3xFLAG-GFP11

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします（Schedule AとSchedule Bを入手[link])。

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)。

提供依頼

手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
提供依頼書 [Word

]
GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします（Schedule AとSchedule Bを入手[link])。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18575	pSFL73 pRS314-GPDp-3xFLAG-GFP11	DNA solution

original	Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Featured content	Visualizing multiple inter-organelle contact sites (English text)

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pSFL73 pRS314-GPDp-3xFLAG-GFP11

Distribution information

References and tips

Sequence information

References

Primer: HB-NOS_R Region: T7 pro, insert 5' (GPD pro, 3xFLAG-GFP11 5') Sequence file: RDB18575_B0J5a.seq
>D07393A9_B0J5_1_HB-NOS_R_A04_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNN ANNNNGGGTN NNGCCNGGGT TTTCCCNGTC NCGACGTTGT 61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA 121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT 181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA 241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC 301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA 361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG 421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG 481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT 541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA 601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG 661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA 721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCTCT 781 AGAACTAGTG GATCCTCTGC CGGTGGTGAN TACAAAGACC ATGATGGCGA TTATAAGGAT 841 CATGACATTG ACTATAANGA TGATGACGAT AAAGGNGGAA GTGGTGGANG NTCAACTTNC 901 AGANATCACA TGGNTTTGNA TGNNNACGTC ANNGNTGCNN NGNNNNANAT AAGAATTCNA 961 NNTCNNGCTT ATCGANANNN NCNANCNNCN NNNCNNNNNA NNNNNNTNNN GNNNNNNNTN 1021 NNNNTNNNNN NCCNNNCNCN CNNNNNNNNN N //
Primer: SV40pA3'Element Reverse Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3') Sequence file: RDB18575_B0J5b.seq
>D07393A9_B0J5_1_SV40A3'elementReverse_B04_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCCNGCNAC GCGGCCTTTT TACGGTTCCT 61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC 241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT 361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA 421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT 481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA 541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA 601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT 661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG 721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA 781 TGTTATCCCT GCAGCATTGA CGTATTCATG CNAAACCNTG TGATCTCTGG AAGTTGANCC 841 NCCACCACTT CCNCCTTTAT CGNCATCATC CTTATAGTCN ANGTCNTGAT CCNTNATAAT 901 CGCCNTCNTG GNCTTTGNNA TCNNCANCGG NANANGNNCC NCNANTTCTA GANCGGCCGC 961 NTCCNNCGAA ACTAANTTNT GGNGTTTTAA AACTNAAAAA AAANNANTNN NNNNAAANNN 1021 NNNNNNN //
Primer: TRP1pro_F Region: TRP1 Sequence file: RDB18575_B0J5c.seq
>D07393A9_B0J5_1_TRP1pro_F_G04_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNAGNNNN GCTTGGANTN NGTCTGTTAT TAATTTCACA 61 GGTAGTTCTG GTCCATTGGT GAAAGTTTGC GGCTTGCAGA GCACAGAGGC CGCAGAATGT 121 GCTCTAGATT CCGATGCTGA CTTGCTGGGT ATTATATGTG TGCCCAATAG AAAGAGAACA 181 ATTGACCCGG TTATTGCAAG GAAAATTTCA AGTCTTGTAA AAGCATATAA AAATAGTTCA 241 GGCACTCCGA AATACTTGGT TGGCGTGTTT CGTAATCAAC CTAAGGAGGA TGTTTTGGCT 301 CTGGTCAATG ATTACGGCAT TGATATCGTC CAACTGCATG GAGATGAGTC GTGGCAAGAA 361 TACCAAGAGT TCCTCGGTTT GCCAGTTATT AAAAGACTCG TATTTCCAAA AGACTGCAAC 421 ATACTACTCA GTGCAGCTTC ACAGAAACCT CATTCGTTTA TTCCCTTGTT TGATTCAGAA 481 GCAGGTGGGA CAGGTGAACT TTTGGATTGG AACTCGATTT CTGACTGGGT TGGAAGGCAA 541 GAGAGCCCCG AAAGCTTACA TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT 601 GATGCGCTTA GATTAAATGG CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT 661 GGTGTAAAAG ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT 721 ACTGAGTAGT ATTTATTTAA GTATTGTTTG TGCACTTGCC GATCTATGCG GTGTGAAATA 781 CCGCACAGAT GCGTAANGAG AAAATACCGC ATCANGAAAT TGTAAGCGTT AATATTTTGT 841 TAAAATTCGC GTTAAATTTT TGTTAANTCA GCTCATTTTT TTAACCNANA GGNCNAANTC 901 GGCAAANTCC NTTATAAATC AAAAGANTAG ANCGANATAG GGTTGANTGN TGNTNCNGTT 961 TGNNNNNNGN NTNNNCTATT AAANAANGNN NNNTNNNNCN NCNANNNNNN AAAAANCNNN 1021 NNNTNNNNNN ANNNNNNNNN N //
Primer: CYC1ter_R Region: insert mid (CYC1 ter, 3xFLAG-GFP11, GPD pro) Sequence file: RDB18575_B0J5d.seq
>D07393A9_B0J5_1_CYC1Ter_R_D04_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN CGGNNGTGGG GGGNNGGCGT GNNTGTAAGC GTGACATAAC 61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTTATC 121 CCTGCAGCAT TGACGTATTC ATGCAAAACC ATGTGATCTC TGGAAGTTGA ACCTCCACCA 181 CTTCCGCCTT TATCGTCATC ATCCTTATAG TCAATGTCAT GATCCTTATA ATCGCCATCA 241 TGGTCTTTGT AATCACCACC GGCAGAGGAT CCACTAGTTC TAGAGCGGCC GCATCCGTCG 301 AAACTAAGTT CTGGTGTTTT AAAACTAAAA AAAAGACTAA CTATAAAAGT AGAATTTAAG 361 AAGTTTAAGA AATAGATTTA CAGAATTACA ATCAATACCT ACCGTCTTTA TATACTTATT 421 AGTCAAGTAG GGGAATAATT TCAGGGAACT GGTTTCAACC TTTTTTTTCA GCTTTTTCCA 481 AATCAGAGAG AGCAGAAGGT AATAGAAGGT GTAAGAAAAT GAGATAGATA CATGCGTGGG 541 TCAATTGCCT TGTGTCATCA TTTACTCCAG GCAGGTTGCA TCACTCCATT GAGGTTGTGC 601 CCGTTTTTTG CCTGTTTGTG CCCCTGTTCT CTGTAGTTGC GCTAAGAGAA TGGACCTATG 661 AACTGATGGT TGGTGAAGAA AACAATATTT TGGTGCTGGG ATTCTTTTTT TTTCTGGATG 721 CCAGCTTAAA AAGCGGGCTC CATTATATTT AGTGGATGCC AGGAATAAAC TGTTCACCCA 781 GACACCTACG ATGTTATATA TTCTGTGTAA CCCGCNCCCT ATTTTGGGCA TGTACGGGTT 841 ACAGCANAAT TNAAANGNTA ATTTTTTTGA CTAAATAAAG NTAGGAAAAT CACNACTANT 901 AATTATTTAN NNANTCTTTG AAANGGNNNN NANNGATANN GANAAACTGA NCTNNNNTTC 961 NNCCNATANN GANTCNNANN ANNNTNNNNN NGNNNNNCNN TTNNNNANNN NNNNNNN //
Primer: Amp_R Region: AmpR pro, CEN_ARS Sequence file: RDB18575_B0J5e.seq
>D07393A9_B0J5_1_Amp_R_E04_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNANN NNANNCNTTN ATNNGGGTTA TTGTCTCNTG AGCGGATACA 61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 121 TGCCACCTGG GTCCTTTTCA TCACGTGCTA TAAAAATAAT TATAATTTAA ATTTTTTAAT 181 ATAAATATAT AAATTAAAAA TAGAAAGTAA AAAAAGAAAT TAAAGAAAAA ATAGTTTTTG 241 TTTTCCGAAG ATGTAAAAGA CTCTAGGGGG ATCGCCAACA AATACTACCT TTTATCTTGC 301 TCTTCCTGCT CTCAGGTATT AATGCCGAAT TGTTTCATCT TGTCTGTGTA GAAGACCACA 361 CACGAAAATC CTGTGATTTT ACATTTTACT TATCGTTAAT CGAATGTATA TCTATTTAAT 421 CTGCTTTTCT TGTCTAATAA ATATATATGT AAAGTACGCT TTTTGTTGAA ATTTTTTAAA 481 CCTTTGTTTA TTTTTTTTTC TTCATTCCGT AACTCTTCTA CCTTCTTTAT TTACTTTCTA 541 AAATCCAAAT ACAAAACATA AAAATAAATA AACACAGAGT AAATTCCCAA ATTATTCCAT 601 CATTAAAAGA TACGAGGCGC GTGTAAGTTA CAGGCAAGCG ATCCGTCTAA GAAACCATTA 661 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT 721 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 781 TGTAAGCGGA NGCCGGGAGC AGANAAGCCC GTCNGGGCGC GTCAGCGGGT GTTGGCGGGN 841 GTCGGGGCTG GCTTAACTAT GCGGCNTCNN ANCANANNGN ACTGANAGTG NNNCATAGAT 901 CACGANATTA CTATATATAT NATATAGGAA NNCATTTNAT AGAANNNCNT CGNAATATAT 961 GTGTANTTTG CNGTTATGNN GCCNNANGGN NNTANTGNNN NANATTCTTT ANTGAAAAAN 1021 ANNNNGNNNN CNTNNNNNNN NNN //