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pSFL73 pRS314-GPDp-3xFLAG-GFP11

Expression vector of 3xFLAG-GFP11.

Catalog number RDB18575
Resource name pSFL73 pRS314-GPDp-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of 3xFLAG-GFP11, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Ampicillin (E. coli), TRP1 (S. cerevisiae).
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kakimoto, Y. et al. Sci. Rep. 8:6175, 2018)
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Schedule A (GFP-A) [Link]
Remarks
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RDB18575 pSFL73 pRS314-GPDp-3xFLAG-GFP11 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18575_B0J5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R
Region: T7 pro, insert 5' (GPD pro, 3xFLAG-GFP11 5')
Sequence file: RDB18575_B0J5a.seq check
>D07393A9_B0J5_1_HB-NOS_R_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNNN ANNNNGGGTN NNGCCNGGGT TTTCCCNGTC NCGACGTTGT
   61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA
  121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
  181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
  241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
  301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
  361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
  421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
  481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
  541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGGAAAAAG CTGAAAAAAA
  601 AGGTTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGACGG
  661 TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC TACTTTTATA
  721 GTTAGTCTTT TTTTTAGTTT TAAAACACCA GAACTTAGTT TCGACGGATG CGGCCGCTCT
  781 AGAACTAGTG GATCCTCTGC CGGTGGTGAN TACAAAGACC ATGATGGCGA TTATAAGGAT
  841 CATGACATTG ACTATAANGA TGATGACGAT AAAGGNGGAA GTGGTGGANG NTCAACTTNC
  901 AGANATCACA TGGNTTTGNA TGNNNACGTC ANNGNTGCNN NGNNNNANAT AAGAATTCNA
  961 NNTCNNGCTT ATCGANANNN NCNANCNNCN NNNCNNNNNA NNNNNNTNNN GNNNNNNNTN
1021 NNNNTNNNNN NCCNNNCNCN CNNNNNNNNN N
//
Primer: SV40pA3'Element Reverse
Region: T3 pro, insert 3' (CYC1 ter, 3xFLAG-GFP11 3')
Sequence file: RDB18575_B0J5b.seq check
>D07393A9_B0J5_1_SV40A3'elementReverse_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGCCNGCNAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
  181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
  241 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
  301 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
  361 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
  421 CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT
  481 GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC CTTCTCAAGC AAGGTTTTCA
  541 GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA AAGAAAAATT TGAAATATAA
  601 ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT AGGGACCTAG ACTTCAGGTT
  661 GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG AGGGCGTGAA TGTAAGCGTG
  721 ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCTTA
  781 TGTTATCCCT GCAGCATTGA CGTATTCATG CNAAACCNTG TGATCTCTGG AAGTTGANCC
  841 NCCACCACTT CCNCCTTTAT CGNCATCATC CTTATAGTCN ANGTCNTGAT CCNTNATAAT
  901 CGCCNTCNTG GNCTTTGNNA TCNNCANCGG NANANGNNCC NCNANTTCTA GANCGGCCGC
  961 NTCCNNCGAA ACTAANTTNT GGNGTTTTAA AACTNAAAAA AAANNANTNN NNNNAAANNN
1021 NNNNNNN
//
Primer: TRP1pro_F
Region: TRP1
Sequence file: RDB18575_B0J5c.seq check
>D07393A9_B0J5_1_TRP1pro_F_G04_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNAGNNNN GCTTGGANTN NGTCTGTTAT TAATTTCACA
   61 GGTAGTTCTG GTCCATTGGT GAAAGTTTGC GGCTTGCAGA GCACAGAGGC CGCAGAATGT
  121 GCTCTAGATT CCGATGCTGA CTTGCTGGGT ATTATATGTG TGCCCAATAG AAAGAGAACA
  181 ATTGACCCGG TTATTGCAAG GAAAATTTCA AGTCTTGTAA AAGCATATAA AAATAGTTCA
  241 GGCACTCCGA AATACTTGGT TGGCGTGTTT CGTAATCAAC CTAAGGAGGA TGTTTTGGCT
  301 CTGGTCAATG ATTACGGCAT TGATATCGTC CAACTGCATG GAGATGAGTC GTGGCAAGAA
  361 TACCAAGAGT TCCTCGGTTT GCCAGTTATT AAAAGACTCG TATTTCCAAA AGACTGCAAC
  421 ATACTACTCA GTGCAGCTTC ACAGAAACCT CATTCGTTTA TTCCCTTGTT TGATTCAGAA
  481 GCAGGTGGGA CAGGTGAACT TTTGGATTGG AACTCGATTT CTGACTGGGT TGGAAGGCAA
  541 GAGAGCCCCG AAAGCTTACA TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT
  601 GATGCGCTTA GATTAAATGG CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT
  661 GGTGTAAAAG ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT
  721 ACTGAGTAGT ATTTATTTAA GTATTGTTTG TGCACTTGCC GATCTATGCG GTGTGAAATA
  781 CCGCACAGAT GCGTAANGAG AAAATACCGC ATCANGAAAT TGTAAGCGTT AATATTTTGT
  841 TAAAATTCGC GTTAAATTTT TGTTAANTCA GCTCATTTTT TTAACCNANA GGNCNAANTC
  901 GGCAAANTCC NTTATAAATC AAAAGANTAG ANCGANATAG GGTTGANTGN TGNTNCNGTT
  961 TGNNNNNNGN NTNNNCTATT AAANAANGNN NNNTNNNNCN NCNANNNNNN AAAAANCNNN
1021 NNNTNNNNNN ANNNNNNNNN N
//
Primer: CYC1ter_R
Region: insert mid (CYC1 ter, 3xFLAG-GFP11, GPD pro)
Sequence file: RDB18575_B0J5d.seq check
>D07393A9_B0J5_1_CYC1Ter_R_D04_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CGGNNGTGGG GGGNNGGCGT GNNTGTAAGC GTGACATAAC
   61 TAATTACATG ACTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC TTATGTTATC
  121 CCTGCAGCAT TGACGTATTC ATGCAAAACC ATGTGATCTC TGGAAGTTGA ACCTCCACCA
  181 CTTCCGCCTT TATCGTCATC ATCCTTATAG TCAATGTCAT GATCCTTATA ATCGCCATCA
  241 TGGTCTTTGT AATCACCACC GGCAGAGGAT CCACTAGTTC TAGAGCGGCC GCATCCGTCG
  301 AAACTAAGTT CTGGTGTTTT AAAACTAAAA AAAAGACTAA CTATAAAAGT AGAATTTAAG
  361 AAGTTTAAGA AATAGATTTA CAGAATTACA ATCAATACCT ACCGTCTTTA TATACTTATT
  421 AGTCAAGTAG GGGAATAATT TCAGGGAACT GGTTTCAACC TTTTTTTTCA GCTTTTTCCA
  481 AATCAGAGAG AGCAGAAGGT AATAGAAGGT GTAAGAAAAT GAGATAGATA CATGCGTGGG
  541 TCAATTGCCT TGTGTCATCA TTTACTCCAG GCAGGTTGCA TCACTCCATT GAGGTTGTGC
  601 CCGTTTTTTG CCTGTTTGTG CCCCTGTTCT CTGTAGTTGC GCTAAGAGAA TGGACCTATG
  661 AACTGATGGT TGGTGAAGAA AACAATATTT TGGTGCTGGG ATTCTTTTTT TTTCTGGATG
  721 CCAGCTTAAA AAGCGGGCTC CATTATATTT AGTGGATGCC AGGAATAAAC TGTTCACCCA
  781 GACACCTACG ATGTTATATA TTCTGTGTAA CCCGCNCCCT ATTTTGGGCA TGTACGGGTT
  841 ACAGCANAAT TNAAANGNTA ATTTTTTTGA CTAAATAAAG NTAGGAAAAT CACNACTANT
  901 AATTATTTAN NNANTCTTTG AAANGGNNNN NANNGATANN GANAAACTGA NCTNNNNTTC
961 NNCCNATANN GANTCNNANN ANNNTNNNNN NGNNNNNCNN TTNNNNANNN NNNNNNN
//
Primer: Amp_R
Region: AmpR pro, CEN_ARS
Sequence file: RDB18575_B0J5e.seq check
>D07393A9_B0J5_1_Amp_R_E04_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNANN NNANNCNTTN ATNNGGGTTA TTGTCTCNTG AGCGGATACA
   61 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
  121 TGCCACCTGG GTCCTTTTCA TCACGTGCTA TAAAAATAAT TATAATTTAA ATTTTTTAAT
  181 ATAAATATAT AAATTAAAAA TAGAAAGTAA AAAAAGAAAT TAAAGAAAAA ATAGTTTTTG
  241 TTTTCCGAAG ATGTAAAAGA CTCTAGGGGG ATCGCCAACA AATACTACCT TTTATCTTGC
  301 TCTTCCTGCT CTCAGGTATT AATGCCGAAT TGTTTCATCT TGTCTGTGTA GAAGACCACA
  361 CACGAAAATC CTGTGATTTT ACATTTTACT TATCGTTAAT CGAATGTATA TCTATTTAAT
  421 CTGCTTTTCT TGTCTAATAA ATATATATGT AAAGTACGCT TTTTGTTGAA ATTTTTTAAA
  481 CCTTTGTTTA TTTTTTTTTC TTCATTCCGT AACTCTTCTA CCTTCTTTAT TTACTTTCTA
  541 AAATCCAAAT ACAAAACATA AAAATAAATA AACACAGAGT AAATTCCCAA ATTATTCCAT
  601 CATTAAAAGA TACGAGGCGC GTGTAAGTTA CAGGCAAGCG ATCCGTCTAA GAAACCATTA
  661 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT
  721 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC
  781 TGTAAGCGGA NGCCGGGAGC AGANAAGCCC GTCNGGGCGC GTCAGCGGGT GTTGGCGGGN
  841 GTCGGGGCTG GCTTAACTAT GCGGCNTCNN ANCANANNGN ACTGANAGTG NNNCATAGAT
  901 CACGANATTA CTATATATAT NATATAGGAA NNCATTTNAT AGAANNNCNT CGNAATATAT
  961 GTGTANTTTG CNGTTATGNN GCCNNANGGN NNTANTGNNN NANATTCTTT ANTGAAAAAN
1021 ANNNNGNNNN CNTNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150.

2021.01.21

GNP_filter3_RDBDEP_html_201222.pl